Job ID = 6366141 SRX = SRX065657 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:47:11 prefetch.2.10.7: 1) Downloading 'SRR217363'... 2020-06-15T22:47:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:47:34 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:47:35 prefetch.2.10.7: 'SRR217363' is valid 2020-06-15T22:47:35 prefetch.2.10.7: 1) 'SRR217363' was downloaded successfully Read 4582326 spots for SRR217363/SRR217363.sra Written 4582326 spots for SRR217363/SRR217363.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 4582326 reads; of these: 4582326 (100.00%) were unpaired; of these: 2525371 (55.11%) aligned 0 times 1815392 (39.62%) aligned exactly 1 time 241563 (5.27%) aligned >1 times 44.89% overall alignment rate Time searching: 00:00:30 Overall time: 00:00:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 340394 / 2056955 = 0.1655 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:49:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:49:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:49:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:49:16: 1000000 INFO @ Tue, 16 Jun 2020 07:49:20: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:49:20: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:49:20: #1 total tags in treatment: 1716561 INFO @ Tue, 16 Jun 2020 07:49:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:49:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:49:20: #1 tags after filtering in treatment: 1716561 INFO @ Tue, 16 Jun 2020 07:49:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:49:20: #1 finished! INFO @ Tue, 16 Jun 2020 07:49:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:49:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:49:20: #2 number of paired peaks: 3057 INFO @ Tue, 16 Jun 2020 07:49:20: start model_add_line... INFO @ Tue, 16 Jun 2020 07:49:20: start X-correlation... INFO @ Tue, 16 Jun 2020 07:49:20: end of X-cor INFO @ Tue, 16 Jun 2020 07:49:20: #2 finished! INFO @ Tue, 16 Jun 2020 07:49:20: #2 predicted fragment length is 156 bps INFO @ Tue, 16 Jun 2020 07:49:20: #2 alternative fragment length(s) may be 156 bps INFO @ Tue, 16 Jun 2020 07:49:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.05_model.r INFO @ Tue, 16 Jun 2020 07:49:20: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:49:20: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:49:24: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:49:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:49:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:49:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.05_summits.bed INFO @ Tue, 16 Jun 2020 07:49:26: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (5454 records, 4 fields): 7 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:49:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:49:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:49:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:49:47: 1000000 INFO @ Tue, 16 Jun 2020 07:49:52: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:49:52: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:49:52: #1 total tags in treatment: 1716561 INFO @ Tue, 16 Jun 2020 07:49:52: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:49:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:49:52: #1 tags after filtering in treatment: 1716561 INFO @ Tue, 16 Jun 2020 07:49:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:49:52: #1 finished! INFO @ Tue, 16 Jun 2020 07:49:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:49:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:49:52: #2 number of paired peaks: 3057 INFO @ Tue, 16 Jun 2020 07:49:52: start model_add_line... INFO @ Tue, 16 Jun 2020 07:49:52: start X-correlation... INFO @ Tue, 16 Jun 2020 07:49:52: end of X-cor INFO @ Tue, 16 Jun 2020 07:49:52: #2 finished! INFO @ Tue, 16 Jun 2020 07:49:52: #2 predicted fragment length is 156 bps INFO @ Tue, 16 Jun 2020 07:49:52: #2 alternative fragment length(s) may be 156 bps INFO @ Tue, 16 Jun 2020 07:49:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.10_model.r INFO @ Tue, 16 Jun 2020 07:49:52: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:49:52: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:49:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:49:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:49:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:49:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.10_summits.bed INFO @ Tue, 16 Jun 2020 07:49:58: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2894 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:50:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:50:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:50:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:50:16: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:50:20: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:50:20: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:50:20: #1 total tags in treatment: 1716561 INFO @ Tue, 16 Jun 2020 07:50:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:50:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:50:20: #1 tags after filtering in treatment: 1716561 INFO @ Tue, 16 Jun 2020 07:50:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:50:20: #1 finished! INFO @ Tue, 16 Jun 2020 07:50:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:50:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:50:21: #2 number of paired peaks: 3057 INFO @ Tue, 16 Jun 2020 07:50:21: start model_add_line... INFO @ Tue, 16 Jun 2020 07:50:21: start X-correlation... INFO @ Tue, 16 Jun 2020 07:50:21: end of X-cor INFO @ Tue, 16 Jun 2020 07:50:21: #2 finished! INFO @ Tue, 16 Jun 2020 07:50:21: #2 predicted fragment length is 156 bps INFO @ Tue, 16 Jun 2020 07:50:21: #2 alternative fragment length(s) may be 156 bps INFO @ Tue, 16 Jun 2020 07:50:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.20_model.r INFO @ Tue, 16 Jun 2020 07:50:21: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:50:21: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:50:25: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:50:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:50:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:50:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065657/SRX065657.20_summits.bed INFO @ Tue, 16 Jun 2020 07:50:27: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1144 records, 4 fields): 3 millis CompletedMACS2peakCalling