Job ID = 6366130 SRX = SRX065646 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:33:36 prefetch.2.10.7: 1) Downloading 'SRR217352'... 2020-06-15T22:33:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:34:04 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:34:04 prefetch.2.10.7: 'SRR217352' is valid 2020-06-15T22:34:04 prefetch.2.10.7: 1) 'SRR217352' was downloaded successfully Read 4792376 spots for SRR217352/SRR217352.sra Written 4792376 spots for SRR217352/SRR217352.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:35 4792376 reads; of these: 4792376 (100.00%) were unpaired; of these: 2403284 (50.15%) aligned 0 times 1991523 (41.56%) aligned exactly 1 time 397569 (8.30%) aligned >1 times 49.85% overall alignment rate Time searching: 00:00:35 Overall time: 00:00:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 74878 / 2389092 = 0.0313 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:35:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:35:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:35:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:36:00: 1000000 INFO @ Tue, 16 Jun 2020 07:36:05: 2000000 INFO @ Tue, 16 Jun 2020 07:36:07: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:36:07: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:36:07: #1 total tags in treatment: 2314214 INFO @ Tue, 16 Jun 2020 07:36:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:36:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:36:07: #1 tags after filtering in treatment: 2314214 INFO @ Tue, 16 Jun 2020 07:36:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:36:07: #1 finished! INFO @ Tue, 16 Jun 2020 07:36:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:36:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:36:07: #2 number of paired peaks: 387 WARNING @ Tue, 16 Jun 2020 07:36:07: Fewer paired peaks (387) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 387 pairs to build model! INFO @ Tue, 16 Jun 2020 07:36:07: start model_add_line... INFO @ Tue, 16 Jun 2020 07:36:07: start X-correlation... INFO @ Tue, 16 Jun 2020 07:36:07: end of X-cor INFO @ Tue, 16 Jun 2020 07:36:07: #2 finished! INFO @ Tue, 16 Jun 2020 07:36:07: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 07:36:07: #2 alternative fragment length(s) may be 4,34,440,525,541,544,563 bps INFO @ Tue, 16 Jun 2020 07:36:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.05_model.r WARNING @ Tue, 16 Jun 2020 07:36:07: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:36:07: #2 You may need to consider one of the other alternative d(s): 4,34,440,525,541,544,563 WARNING @ Tue, 16 Jun 2020 07:36:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:36:07: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:36:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:36:12: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:36:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:36:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:36:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.05_summits.bed INFO @ Tue, 16 Jun 2020 07:36:15: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (261 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:36:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:36:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:36:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:36:29: 1000000 INFO @ Tue, 16 Jun 2020 07:36:35: 2000000 INFO @ Tue, 16 Jun 2020 07:36:36: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:36:36: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:36:36: #1 total tags in treatment: 2314214 INFO @ Tue, 16 Jun 2020 07:36:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:36:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:36:36: #1 tags after filtering in treatment: 2314214 INFO @ Tue, 16 Jun 2020 07:36:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:36:36: #1 finished! INFO @ Tue, 16 Jun 2020 07:36:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:36:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:36:37: #2 number of paired peaks: 387 WARNING @ Tue, 16 Jun 2020 07:36:37: Fewer paired peaks (387) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 387 pairs to build model! INFO @ Tue, 16 Jun 2020 07:36:37: start model_add_line... INFO @ Tue, 16 Jun 2020 07:36:37: start X-correlation... INFO @ Tue, 16 Jun 2020 07:36:37: end of X-cor INFO @ Tue, 16 Jun 2020 07:36:37: #2 finished! INFO @ Tue, 16 Jun 2020 07:36:37: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 07:36:37: #2 alternative fragment length(s) may be 4,34,440,525,541,544,563 bps INFO @ Tue, 16 Jun 2020 07:36:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.10_model.r WARNING @ Tue, 16 Jun 2020 07:36:37: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:36:37: #2 You may need to consider one of the other alternative d(s): 4,34,440,525,541,544,563 WARNING @ Tue, 16 Jun 2020 07:36:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:36:37: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:36:37: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:36:42: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:36:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:36:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:36:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.10_summits.bed INFO @ Tue, 16 Jun 2020 07:36:45: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (116 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:36:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:36:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:36:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:36:59: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:37:05: 2000000 INFO @ Tue, 16 Jun 2020 07:37:06: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:37:06: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:37:06: #1 total tags in treatment: 2314214 INFO @ Tue, 16 Jun 2020 07:37:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:37:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:37:06: #1 tags after filtering in treatment: 2314214 INFO @ Tue, 16 Jun 2020 07:37:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:37:06: #1 finished! INFO @ Tue, 16 Jun 2020 07:37:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:37:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:37:07: #2 number of paired peaks: 387 WARNING @ Tue, 16 Jun 2020 07:37:07: Fewer paired peaks (387) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 387 pairs to build model! INFO @ Tue, 16 Jun 2020 07:37:07: start model_add_line... INFO @ Tue, 16 Jun 2020 07:37:07: start X-correlation... INFO @ Tue, 16 Jun 2020 07:37:07: end of X-cor INFO @ Tue, 16 Jun 2020 07:37:07: #2 finished! INFO @ Tue, 16 Jun 2020 07:37:07: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 07:37:07: #2 alternative fragment length(s) may be 4,34,440,525,541,544,563 bps INFO @ Tue, 16 Jun 2020 07:37:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.20_model.r WARNING @ Tue, 16 Jun 2020 07:37:07: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:37:07: #2 You may need to consider one of the other alternative d(s): 4,34,440,525,541,544,563 WARNING @ Tue, 16 Jun 2020 07:37:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:37:07: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:37:07: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:37:12: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:37:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:37:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:37:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065646/SRX065646.20_summits.bed INFO @ Tue, 16 Jun 2020 07:37:15: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (22 records, 4 fields): 1 millis CompletedMACS2peakCalling