Job ID = 6366127 SRX = SRX065643 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:28:15 prefetch.2.10.7: 1) Downloading 'SRR217349'... 2020-06-15T22:28:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:28:38 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:28:38 prefetch.2.10.7: 'SRR217349' is valid 2020-06-15T22:28:38 prefetch.2.10.7: 1) 'SRR217349' was downloaded successfully Read 3033149 spots for SRR217349/SRR217349.sra Written 3033149 spots for SRR217349/SRR217349.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:24 3033149 reads; of these: 3033149 (100.00%) were unpaired; of these: 847925 (27.96%) aligned 0 times 1816659 (59.89%) aligned exactly 1 time 368565 (12.15%) aligned >1 times 72.04% overall alignment rate Time searching: 00:00:24 Overall time: 00:00:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 51114 / 2185224 = 0.0234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:30:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:30:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:30:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:30:12: 1000000 INFO @ Tue, 16 Jun 2020 07:30:17: 2000000 INFO @ Tue, 16 Jun 2020 07:30:18: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:30:18: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:30:18: #1 total tags in treatment: 2134110 INFO @ Tue, 16 Jun 2020 07:30:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:30:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:30:18: #1 tags after filtering in treatment: 2134110 INFO @ Tue, 16 Jun 2020 07:30:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:30:18: #1 finished! INFO @ Tue, 16 Jun 2020 07:30:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:30:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:30:18: #2 number of paired peaks: 444 WARNING @ Tue, 16 Jun 2020 07:30:18: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Tue, 16 Jun 2020 07:30:18: start model_add_line... INFO @ Tue, 16 Jun 2020 07:30:18: start X-correlation... INFO @ Tue, 16 Jun 2020 07:30:18: end of X-cor INFO @ Tue, 16 Jun 2020 07:30:18: #2 finished! INFO @ Tue, 16 Jun 2020 07:30:18: #2 predicted fragment length is 35 bps INFO @ Tue, 16 Jun 2020 07:30:18: #2 alternative fragment length(s) may be 35 bps INFO @ Tue, 16 Jun 2020 07:30:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.05_model.r WARNING @ Tue, 16 Jun 2020 07:30:18: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:30:18: #2 You may need to consider one of the other alternative d(s): 35 WARNING @ Tue, 16 Jun 2020 07:30:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:30:18: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:30:18: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:30:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:30:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:30:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:30:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.05_summits.bed INFO @ Tue, 16 Jun 2020 07:30:25: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (263 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:30:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:30:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:30:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:30:42: 1000000 INFO @ Tue, 16 Jun 2020 07:30:49: 2000000 INFO @ Tue, 16 Jun 2020 07:30:50: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:30:50: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:30:50: #1 total tags in treatment: 2134110 INFO @ Tue, 16 Jun 2020 07:30:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:30:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:30:50: #1 tags after filtering in treatment: 2134110 INFO @ Tue, 16 Jun 2020 07:30:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:30:50: #1 finished! INFO @ Tue, 16 Jun 2020 07:30:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:30:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:30:50: #2 number of paired peaks: 444 WARNING @ Tue, 16 Jun 2020 07:30:50: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Tue, 16 Jun 2020 07:30:50: start model_add_line... INFO @ Tue, 16 Jun 2020 07:30:50: start X-correlation... INFO @ Tue, 16 Jun 2020 07:30:50: end of X-cor INFO @ Tue, 16 Jun 2020 07:30:50: #2 finished! INFO @ Tue, 16 Jun 2020 07:30:50: #2 predicted fragment length is 35 bps INFO @ Tue, 16 Jun 2020 07:30:50: #2 alternative fragment length(s) may be 35 bps INFO @ Tue, 16 Jun 2020 07:30:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.10_model.r WARNING @ Tue, 16 Jun 2020 07:30:50: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:30:50: #2 You may need to consider one of the other alternative d(s): 35 WARNING @ Tue, 16 Jun 2020 07:30:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:30:50: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:30:50: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:30:55: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:30:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:30:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:30:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.10_summits.bed INFO @ Tue, 16 Jun 2020 07:30:57: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (100 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:31:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:31:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:31:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:31:12: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:31:19: 2000000 INFO @ Tue, 16 Jun 2020 07:31:20: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:31:20: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:31:20: #1 total tags in treatment: 2134110 INFO @ Tue, 16 Jun 2020 07:31:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:31:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:31:20: #1 tags after filtering in treatment: 2134110 INFO @ Tue, 16 Jun 2020 07:31:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:31:20: #1 finished! INFO @ Tue, 16 Jun 2020 07:31:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:31:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:31:20: #2 number of paired peaks: 444 WARNING @ Tue, 16 Jun 2020 07:31:20: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Tue, 16 Jun 2020 07:31:20: start model_add_line... INFO @ Tue, 16 Jun 2020 07:31:20: start X-correlation... INFO @ Tue, 16 Jun 2020 07:31:20: end of X-cor INFO @ Tue, 16 Jun 2020 07:31:20: #2 finished! INFO @ Tue, 16 Jun 2020 07:31:20: #2 predicted fragment length is 35 bps INFO @ Tue, 16 Jun 2020 07:31:20: #2 alternative fragment length(s) may be 35 bps INFO @ Tue, 16 Jun 2020 07:31:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.20_model.r WARNING @ Tue, 16 Jun 2020 07:31:20: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:31:20: #2 You may need to consider one of the other alternative d(s): 35 WARNING @ Tue, 16 Jun 2020 07:31:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:31:20: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:31:20: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:31:25: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:31:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:31:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:31:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065643/SRX065643.20_summits.bed INFO @ Tue, 16 Jun 2020 07:31:27: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (23 records, 4 fields): 1 millis CompletedMACS2peakCalling