Job ID = 6366033 SRX = SRX054261 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:30:59 prefetch.2.10.7: 1) Downloading 'SRR164268'... 2020-06-15T22:30:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:31:47 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:31:47 prefetch.2.10.7: 'SRR164268' is valid 2020-06-15T22:31:47 prefetch.2.10.7: 1) 'SRR164268' was downloaded successfully Read 7119810 spots for SRR164268/SRR164268.sra Written 7119810 spots for SRR164268/SRR164268.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:33 7119810 reads; of these: 7119810 (100.00%) were unpaired; of these: 5842431 (82.06%) aligned 0 times 1127929 (15.84%) aligned exactly 1 time 149450 (2.10%) aligned >1 times 17.94% overall alignment rate Time searching: 00:00:33 Overall time: 00:00:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 56246 / 1277379 = 0.0440 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:33:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:33:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:33:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:33:37: 1000000 INFO @ Tue, 16 Jun 2020 07:33:38: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:33:38: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:33:38: #1 total tags in treatment: 1221133 INFO @ Tue, 16 Jun 2020 07:33:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:33:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:33:38: #1 tags after filtering in treatment: 1221133 INFO @ Tue, 16 Jun 2020 07:33:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:33:38: #1 finished! INFO @ Tue, 16 Jun 2020 07:33:38: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:33:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:33:38: #2 number of paired peaks: 209 WARNING @ Tue, 16 Jun 2020 07:33:38: Fewer paired peaks (209) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 209 pairs to build model! INFO @ Tue, 16 Jun 2020 07:33:38: start model_add_line... INFO @ Tue, 16 Jun 2020 07:33:38: start X-correlation... INFO @ Tue, 16 Jun 2020 07:33:38: end of X-cor INFO @ Tue, 16 Jun 2020 07:33:38: #2 finished! INFO @ Tue, 16 Jun 2020 07:33:38: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 07:33:38: #2 alternative fragment length(s) may be 36,130,167,342,469,503,552,570 bps INFO @ Tue, 16 Jun 2020 07:33:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.05_model.r WARNING @ Tue, 16 Jun 2020 07:33:38: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:33:38: #2 You may need to consider one of the other alternative d(s): 36,130,167,342,469,503,552,570 WARNING @ Tue, 16 Jun 2020 07:33:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:33:38: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:33:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:33:41: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:33:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:33:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:33:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.05_summits.bed INFO @ Tue, 16 Jun 2020 07:33:42: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (65 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:34:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:34:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:34:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:34:06: 1000000 INFO @ Tue, 16 Jun 2020 07:34:08: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:34:08: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:34:08: #1 total tags in treatment: 1221133 INFO @ Tue, 16 Jun 2020 07:34:08: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:34:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:34:08: #1 tags after filtering in treatment: 1221133 INFO @ Tue, 16 Jun 2020 07:34:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:34:08: #1 finished! INFO @ Tue, 16 Jun 2020 07:34:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:34:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:34:08: #2 number of paired peaks: 209 WARNING @ Tue, 16 Jun 2020 07:34:08: Fewer paired peaks (209) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 209 pairs to build model! INFO @ Tue, 16 Jun 2020 07:34:08: start model_add_line... INFO @ Tue, 16 Jun 2020 07:34:08: start X-correlation... INFO @ Tue, 16 Jun 2020 07:34:08: end of X-cor INFO @ Tue, 16 Jun 2020 07:34:08: #2 finished! INFO @ Tue, 16 Jun 2020 07:34:08: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 07:34:08: #2 alternative fragment length(s) may be 36,130,167,342,469,503,552,570 bps INFO @ Tue, 16 Jun 2020 07:34:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.10_model.r WARNING @ Tue, 16 Jun 2020 07:34:08: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:34:08: #2 You may need to consider one of the other alternative d(s): 36,130,167,342,469,503,552,570 WARNING @ Tue, 16 Jun 2020 07:34:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:34:08: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:34:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:34:10: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:34:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:34:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:34:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.10_summits.bed INFO @ Tue, 16 Jun 2020 07:34:12: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (19 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:34:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:34:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:34:31: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:34:36: 1000000 INFO @ Tue, 16 Jun 2020 07:34:37: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:34:37: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:34:37: #1 total tags in treatment: 1221133 INFO @ Tue, 16 Jun 2020 07:34:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:34:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:34:37: #1 tags after filtering in treatment: 1221133 INFO @ Tue, 16 Jun 2020 07:34:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:34:37: #1 finished! INFO @ Tue, 16 Jun 2020 07:34:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:34:37: #2 number of paired peaks: 209 WARNING @ Tue, 16 Jun 2020 07:34:37: Fewer paired peaks (209) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 209 pairs to build model! INFO @ Tue, 16 Jun 2020 07:34:37: start model_add_line... INFO @ Tue, 16 Jun 2020 07:34:37: start X-correlation... INFO @ Tue, 16 Jun 2020 07:34:37: end of X-cor INFO @ Tue, 16 Jun 2020 07:34:37: #2 finished! INFO @ Tue, 16 Jun 2020 07:34:37: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 07:34:37: #2 alternative fragment length(s) may be 36,130,167,342,469,503,552,570 bps INFO @ Tue, 16 Jun 2020 07:34:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.20_model.r WARNING @ Tue, 16 Jun 2020 07:34:37: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:34:37: #2 You may need to consider one of the other alternative d(s): 36,130,167,342,469,503,552,570 WARNING @ Tue, 16 Jun 2020 07:34:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:34:37: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:34:37: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:34:40: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:34:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:34:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:34:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX054261/SRX054261.20_summits.bed INFO @ Tue, 16 Jun 2020 07:34:42: Done! pass1 - making usageList (2 chroms): 1 millis pass2 - checking and writing primary data (2 records, 4 fields): 0 millis CompletedMACS2peakCalling