Job ID = 6365901 SRX = SRX015099 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:35:51 prefetch.2.10.7: 1) Downloading 'SRR032445'... 2020-06-15T22:35:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:36:20 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:36:20 prefetch.2.10.7: 'SRR032445' is valid 2020-06-15T22:36:20 prefetch.2.10.7: 1) 'SRR032445' was downloaded successfully Read 3175883 spots for SRR032445/SRR032445.sra Written 3175883 spots for SRR032445/SRR032445.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:17 3175883 reads; of these: 3175883 (100.00%) were unpaired; of these: 2737512 (86.20%) aligned 0 times 387189 (12.19%) aligned exactly 1 time 51182 (1.61%) aligned >1 times 13.80% overall alignment rate Time searching: 00:00:17 Overall time: 00:00:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 12460 / 438371 = 0.0284 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:37:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:37:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:37:27: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:37:27: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:37:27: #1 total tags in treatment: 425911 INFO @ Tue, 16 Jun 2020 07:37:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:37:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:37:27: #1 tags after filtering in treatment: 425911 INFO @ Tue, 16 Jun 2020 07:37:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:37:27: #1 finished! INFO @ Tue, 16 Jun 2020 07:37:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:37:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:37:27: #2 number of paired peaks: 305 WARNING @ Tue, 16 Jun 2020 07:37:27: Fewer paired peaks (305) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 305 pairs to build model! INFO @ Tue, 16 Jun 2020 07:37:27: start model_add_line... INFO @ Tue, 16 Jun 2020 07:37:27: start X-correlation... INFO @ Tue, 16 Jun 2020 07:37:27: end of X-cor INFO @ Tue, 16 Jun 2020 07:37:27: #2 finished! INFO @ Tue, 16 Jun 2020 07:37:27: #2 predicted fragment length is 123 bps INFO @ Tue, 16 Jun 2020 07:37:27: #2 alternative fragment length(s) may be 44,123,176,277,298,523,594 bps INFO @ Tue, 16 Jun 2020 07:37:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.05_model.r INFO @ Tue, 16 Jun 2020 07:37:27: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:37:27: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:37:29: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:37:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:37:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:37:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.05_summits.bed INFO @ Tue, 16 Jun 2020 07:37:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (16 records, 4 fields): 0 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:37:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:37:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:37:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:37:57: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:37:57: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:37:57: #1 total tags in treatment: 425911 INFO @ Tue, 16 Jun 2020 07:37:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:37:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:37:57: #1 tags after filtering in treatment: 425911 INFO @ Tue, 16 Jun 2020 07:37:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:37:57: #1 finished! INFO @ Tue, 16 Jun 2020 07:37:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:37:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:37:57: #2 number of paired peaks: 305 WARNING @ Tue, 16 Jun 2020 07:37:57: Fewer paired peaks (305) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 305 pairs to build model! INFO @ Tue, 16 Jun 2020 07:37:57: start model_add_line... INFO @ Tue, 16 Jun 2020 07:37:57: start X-correlation... INFO @ Tue, 16 Jun 2020 07:37:57: end of X-cor INFO @ Tue, 16 Jun 2020 07:37:57: #2 finished! INFO @ Tue, 16 Jun 2020 07:37:57: #2 predicted fragment length is 123 bps INFO @ Tue, 16 Jun 2020 07:37:57: #2 alternative fragment length(s) may be 44,123,176,277,298,523,594 bps INFO @ Tue, 16 Jun 2020 07:37:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.10_model.r INFO @ Tue, 16 Jun 2020 07:37:57: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:37:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:37:58: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:37:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:37:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:37:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.10_summits.bed INFO @ Tue, 16 Jun 2020 07:37:59: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (9 records, 4 fields): 17 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:38:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:38:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:38:25: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:38:27: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:38:27: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:38:27: #1 total tags in treatment: 425911 INFO @ Tue, 16 Jun 2020 07:38:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:38:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:38:27: #1 tags after filtering in treatment: 425911 INFO @ Tue, 16 Jun 2020 07:38:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:38:27: #1 finished! INFO @ Tue, 16 Jun 2020 07:38:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:38:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:38:27: #2 number of paired peaks: 305 WARNING @ Tue, 16 Jun 2020 07:38:27: Fewer paired peaks (305) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 305 pairs to build model! INFO @ Tue, 16 Jun 2020 07:38:27: start model_add_line... INFO @ Tue, 16 Jun 2020 07:38:27: start X-correlation... INFO @ Tue, 16 Jun 2020 07:38:27: end of X-cor INFO @ Tue, 16 Jun 2020 07:38:27: #2 finished! INFO @ Tue, 16 Jun 2020 07:38:27: #2 predicted fragment length is 123 bps INFO @ Tue, 16 Jun 2020 07:38:27: #2 alternative fragment length(s) may be 44,123,176,277,298,523,594 bps INFO @ Tue, 16 Jun 2020 07:38:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.20_model.r INFO @ Tue, 16 Jun 2020 07:38:27: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:38:27: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:38:28: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:38:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:38:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:38:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX015099/SRX015099.20_summits.bed INFO @ Tue, 16 Jun 2020 07:38:29: Done! pass1 - making usageList (2 chroms): 0 millis pass2 - checking and writing primary data (3 records, 4 fields): 1 millis CompletedMACS2peakCalling