Job ID = 6365870 SRX = SRX005627 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:28:44 prefetch.2.10.7: 1) Downloading 'SRR017596'... 2020-06-15T22:28:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:29:14 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:29:14 prefetch.2.10.7: 'SRR017596' is valid 2020-06-15T22:29:14 prefetch.2.10.7: 1) 'SRR017596' was downloaded successfully Read 7292446 spots for SRR017596/SRR017596.sra Written 7292446 spots for SRR017596/SRR017596.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:05 7292446 reads; of these: 7292446 (100.00%) were unpaired; of these: 826788 (11.34%) aligned 0 times 5260039 (72.13%) aligned exactly 1 time 1205619 (16.53%) aligned >1 times 88.66% overall alignment rate Time searching: 00:01:05 Overall time: 00:01:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 550609 / 6465658 = 0.0852 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:32:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:32:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:32:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:32:32: 1000000 INFO @ Tue, 16 Jun 2020 07:32:37: 2000000 INFO @ Tue, 16 Jun 2020 07:32:42: 3000000 INFO @ Tue, 16 Jun 2020 07:32:47: 4000000 INFO @ Tue, 16 Jun 2020 07:32:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:32:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:32:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:32:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:32:57: #1 tag size is determined as 27 bps INFO @ Tue, 16 Jun 2020 07:32:57: #1 tag size = 27 INFO @ Tue, 16 Jun 2020 07:32:57: #1 total tags in treatment: 5915049 INFO @ Tue, 16 Jun 2020 07:32:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:32:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:32:57: #1 tags after filtering in treatment: 5915049 INFO @ Tue, 16 Jun 2020 07:32:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:32:57: #1 finished! INFO @ Tue, 16 Jun 2020 07:32:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:32:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:32:58: #2 number of paired peaks: 241 WARNING @ Tue, 16 Jun 2020 07:32:58: Fewer paired peaks (241) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 241 pairs to build model! INFO @ Tue, 16 Jun 2020 07:32:58: start model_add_line... INFO @ Tue, 16 Jun 2020 07:32:58: start X-correlation... INFO @ Tue, 16 Jun 2020 07:32:58: end of X-cor INFO @ Tue, 16 Jun 2020 07:32:58: #2 finished! INFO @ Tue, 16 Jun 2020 07:32:58: #2 predicted fragment length is 30 bps INFO @ Tue, 16 Jun 2020 07:32:58: #2 alternative fragment length(s) may be 3,30,527 bps INFO @ Tue, 16 Jun 2020 07:32:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.05_model.r WARNING @ Tue, 16 Jun 2020 07:32:58: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:32:58: #2 You may need to consider one of the other alternative d(s): 3,30,527 WARNING @ Tue, 16 Jun 2020 07:32:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:32:58: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:32:58: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:33:02: 1000000 INFO @ Tue, 16 Jun 2020 07:33:08: 2000000 INFO @ Tue, 16 Jun 2020 07:33:12: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:33:14: 3000000 INFO @ Tue, 16 Jun 2020 07:33:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:33:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:33:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.05_summits.bed INFO @ Tue, 16 Jun 2020 07:33:19: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (561 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:33:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:33:25: 5000000 INFO @ Tue, 16 Jun 2020 07:33:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:33:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:33:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:33:31: #1 tag size is determined as 27 bps INFO @ Tue, 16 Jun 2020 07:33:31: #1 tag size = 27 INFO @ Tue, 16 Jun 2020 07:33:31: #1 total tags in treatment: 5915049 INFO @ Tue, 16 Jun 2020 07:33:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:33:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:33:31: #1 tags after filtering in treatment: 5915049 INFO @ Tue, 16 Jun 2020 07:33:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:33:31: #1 finished! INFO @ Tue, 16 Jun 2020 07:33:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:33:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:33:31: #2 number of paired peaks: 241 WARNING @ Tue, 16 Jun 2020 07:33:31: Fewer paired peaks (241) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 241 pairs to build model! INFO @ Tue, 16 Jun 2020 07:33:31: start model_add_line... INFO @ Tue, 16 Jun 2020 07:33:31: start X-correlation... INFO @ Tue, 16 Jun 2020 07:33:31: end of X-cor INFO @ Tue, 16 Jun 2020 07:33:31: #2 finished! INFO @ Tue, 16 Jun 2020 07:33:31: #2 predicted fragment length is 30 bps INFO @ Tue, 16 Jun 2020 07:33:31: #2 alternative fragment length(s) may be 3,30,527 bps INFO @ Tue, 16 Jun 2020 07:33:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.10_model.r WARNING @ Tue, 16 Jun 2020 07:33:31: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:33:31: #2 You may need to consider one of the other alternative d(s): 3,30,527 WARNING @ Tue, 16 Jun 2020 07:33:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:33:31: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:33:31: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:33:32: 1000000 INFO @ Tue, 16 Jun 2020 07:33:37: 2000000 INFO @ Tue, 16 Jun 2020 07:33:42: 3000000 INFO @ Tue, 16 Jun 2020 07:33:45: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:33:47: 4000000 INFO @ Tue, 16 Jun 2020 07:33:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:33:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:33:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.10_summits.bed INFO @ Tue, 16 Jun 2020 07:33:52: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (243 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:33:52: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:33:57: #1 tag size is determined as 27 bps INFO @ Tue, 16 Jun 2020 07:33:57: #1 tag size = 27 INFO @ Tue, 16 Jun 2020 07:33:57: #1 total tags in treatment: 5915049 INFO @ Tue, 16 Jun 2020 07:33:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:33:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:33:57: #1 tags after filtering in treatment: 5915049 INFO @ Tue, 16 Jun 2020 07:33:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:33:57: #1 finished! INFO @ Tue, 16 Jun 2020 07:33:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:33:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:33:57: #2 number of paired peaks: 241 WARNING @ Tue, 16 Jun 2020 07:33:57: Fewer paired peaks (241) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 241 pairs to build model! INFO @ Tue, 16 Jun 2020 07:33:57: start model_add_line... INFO @ Tue, 16 Jun 2020 07:33:58: start X-correlation... INFO @ Tue, 16 Jun 2020 07:33:58: end of X-cor INFO @ Tue, 16 Jun 2020 07:33:58: #2 finished! INFO @ Tue, 16 Jun 2020 07:33:58: #2 predicted fragment length is 30 bps INFO @ Tue, 16 Jun 2020 07:33:58: #2 alternative fragment length(s) may be 3,30,527 bps INFO @ Tue, 16 Jun 2020 07:33:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.20_model.r WARNING @ Tue, 16 Jun 2020 07:33:58: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:33:58: #2 You may need to consider one of the other alternative d(s): 3,30,527 WARNING @ Tue, 16 Jun 2020 07:33:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:33:58: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:33:58: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:34:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:34:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:34:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:34:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX005627/SRX005627.20_summits.bed INFO @ Tue, 16 Jun 2020 07:34:17: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (83 records, 4 fields): 1 millis CompletedMACS2peakCalling