Job ID = 6365838 SRX = ERX1999208 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:42:22 prefetch.2.10.7: 1) Downloading 'ERR1938675'... 2020-06-15T22:42:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:42:36 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:42:36 prefetch.2.10.7: 'ERR1938675' is valid 2020-06-15T22:42:36 prefetch.2.10.7: 1) 'ERR1938675' was downloaded successfully Read 454553 spots for ERR1938675/ERR1938675.sra Written 454553 spots for ERR1938675/ERR1938675.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:05 454553 reads; of these: 454553 (100.00%) were unpaired; of these: 219571 (48.30%) aligned 0 times 170319 (37.47%) aligned exactly 1 time 64663 (14.23%) aligned >1 times 51.70% overall alignment rate Time searching: 00:00:05 Overall time: 00:00:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 20121 / 234982 = 0.0856 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:43:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:43:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:43:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:43:21: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:43:21: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:43:21: #1 total tags in treatment: 214861 INFO @ Tue, 16 Jun 2020 07:43:21: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:43:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:43:21: #1 tags after filtering in treatment: 214860 INFO @ Tue, 16 Jun 2020 07:43:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:43:21: #1 finished! INFO @ Tue, 16 Jun 2020 07:43:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:43:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:43:21: #2 number of paired peaks: 2160 INFO @ Tue, 16 Jun 2020 07:43:21: start model_add_line... INFO @ Tue, 16 Jun 2020 07:43:21: start X-correlation... INFO @ Tue, 16 Jun 2020 07:43:21: end of X-cor INFO @ Tue, 16 Jun 2020 07:43:21: #2 finished! INFO @ Tue, 16 Jun 2020 07:43:21: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 07:43:21: #2 alternative fragment length(s) may be 200 bps INFO @ Tue, 16 Jun 2020 07:43:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.05_model.r INFO @ Tue, 16 Jun 2020 07:43:21: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:43:21: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:43:21: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:43:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:43:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:43:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.05_summits.bed INFO @ Tue, 16 Jun 2020 07:43:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (102 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:43:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:43:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:43:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:43:51: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:43:51: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:43:51: #1 total tags in treatment: 214861 INFO @ Tue, 16 Jun 2020 07:43:51: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:43:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:43:51: #1 tags after filtering in treatment: 214860 INFO @ Tue, 16 Jun 2020 07:43:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:43:51: #1 finished! INFO @ Tue, 16 Jun 2020 07:43:51: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:43:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:43:51: #2 number of paired peaks: 2160 INFO @ Tue, 16 Jun 2020 07:43:51: start model_add_line... INFO @ Tue, 16 Jun 2020 07:43:51: start X-correlation... INFO @ Tue, 16 Jun 2020 07:43:51: end of X-cor INFO @ Tue, 16 Jun 2020 07:43:51: #2 finished! INFO @ Tue, 16 Jun 2020 07:43:51: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 07:43:51: #2 alternative fragment length(s) may be 200 bps INFO @ Tue, 16 Jun 2020 07:43:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.10_model.r INFO @ Tue, 16 Jun 2020 07:43:51: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:43:51: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:43:51: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:43:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:43:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:43:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.10_summits.bed INFO @ Tue, 16 Jun 2020 07:43:52: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (47 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:44:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:44:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:44:20: #1 read treatment tags... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:44:21: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:44:21: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:44:21: #1 total tags in treatment: 214861 INFO @ Tue, 16 Jun 2020 07:44:21: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:44:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:44:21: #1 tags after filtering in treatment: 214860 INFO @ Tue, 16 Jun 2020 07:44:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:44:21: #1 finished! INFO @ Tue, 16 Jun 2020 07:44:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:44:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:44:21: #2 number of paired peaks: 2160 INFO @ Tue, 16 Jun 2020 07:44:21: start model_add_line... INFO @ Tue, 16 Jun 2020 07:44:21: start X-correlation... INFO @ Tue, 16 Jun 2020 07:44:21: end of X-cor INFO @ Tue, 16 Jun 2020 07:44:21: #2 finished! INFO @ Tue, 16 Jun 2020 07:44:21: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 07:44:21: #2 alternative fragment length(s) may be 200 bps INFO @ Tue, 16 Jun 2020 07:44:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.20_model.r INFO @ Tue, 16 Jun 2020 07:44:21: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:44:21: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:44:21: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:44:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:44:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:44:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/ERX1999208/ERX1999208.20_summits.bed INFO @ Tue, 16 Jun 2020 07:44:22: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (16 records, 4 fields): 1 millis CompletedMACS2peakCalling