Job ID = 10165647 SRX = SRX7879254 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42721018 spots for SRR11272869/SRR11272869.sra Written 42721018 spots for SRR11272869/SRR11272869.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165930 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:09 42721018 reads; of these: 42721018 (100.00%) were unpaired; of these: 522639 (1.22%) aligned 0 times 34418961 (80.57%) aligned exactly 1 time 7779418 (18.21%) aligned >1 times 98.78% overall alignment rate Time searching: 00:09:09 Overall time: 00:09:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7899210 / 42198379 = 0.1872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:40: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:40: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:46: 1000000 INFO @ Thu, 08 Oct 2020 19:51:52: 2000000 INFO @ Thu, 08 Oct 2020 19:51:57: 3000000 INFO @ Thu, 08 Oct 2020 19:52:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:09: 5000000 INFO @ Thu, 08 Oct 2020 19:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:10: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:10: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:15: 6000000 INFO @ Thu, 08 Oct 2020 19:52:17: 1000000 INFO @ Thu, 08 Oct 2020 19:52:22: 7000000 INFO @ Thu, 08 Oct 2020 19:52:23: 2000000 INFO @ Thu, 08 Oct 2020 19:52:29: 8000000 INFO @ Thu, 08 Oct 2020 19:52:30: 3000000 INFO @ Thu, 08 Oct 2020 19:52:35: 9000000 INFO @ Thu, 08 Oct 2020 19:52:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:40: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:40: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:42: 10000000 INFO @ Thu, 08 Oct 2020 19:52:43: 5000000 INFO @ Thu, 08 Oct 2020 19:52:47: 1000000 INFO @ Thu, 08 Oct 2020 19:52:49: 11000000 INFO @ Thu, 08 Oct 2020 19:52:50: 6000000 INFO @ Thu, 08 Oct 2020 19:52:53: 2000000 INFO @ Thu, 08 Oct 2020 19:52:56: 12000000 INFO @ Thu, 08 Oct 2020 19:52:57: 7000000 INFO @ Thu, 08 Oct 2020 19:53:00: 3000000 INFO @ Thu, 08 Oct 2020 19:53:03: 13000000 INFO @ Thu, 08 Oct 2020 19:53:04: 8000000 INFO @ Thu, 08 Oct 2020 19:53:07: 4000000 INFO @ Thu, 08 Oct 2020 19:53:10: 14000000 INFO @ Thu, 08 Oct 2020 19:53:11: 9000000 INFO @ Thu, 08 Oct 2020 19:53:14: 5000000 INFO @ Thu, 08 Oct 2020 19:53:17: 15000000 INFO @ Thu, 08 Oct 2020 19:53:18: 10000000 INFO @ Thu, 08 Oct 2020 19:53:21: 6000000 INFO @ Thu, 08 Oct 2020 19:53:24: 16000000 INFO @ Thu, 08 Oct 2020 19:53:25: 11000000 INFO @ Thu, 08 Oct 2020 19:53:28: 7000000 INFO @ Thu, 08 Oct 2020 19:53:30: 17000000 INFO @ Thu, 08 Oct 2020 19:53:32: 12000000 INFO @ Thu, 08 Oct 2020 19:53:34: 8000000 INFO @ Thu, 08 Oct 2020 19:53:37: 18000000 INFO @ Thu, 08 Oct 2020 19:53:39: 13000000 INFO @ Thu, 08 Oct 2020 19:53:41: 9000000 INFO @ Thu, 08 Oct 2020 19:53:44: 19000000 INFO @ Thu, 08 Oct 2020 19:53:46: 14000000 INFO @ Thu, 08 Oct 2020 19:53:48: 10000000 INFO @ Thu, 08 Oct 2020 19:53:51: 20000000 INFO @ Thu, 08 Oct 2020 19:53:52: 15000000 INFO @ Thu, 08 Oct 2020 19:53:55: 11000000 INFO @ Thu, 08 Oct 2020 19:53:58: 21000000 INFO @ Thu, 08 Oct 2020 19:53:59: 16000000 INFO @ Thu, 08 Oct 2020 19:54:01: 12000000 INFO @ Thu, 08 Oct 2020 19:54:05: 22000000 INFO @ Thu, 08 Oct 2020 19:54:06: 17000000 INFO @ Thu, 08 Oct 2020 19:54:08: 13000000 INFO @ Thu, 08 Oct 2020 19:54:12: 23000000 INFO @ Thu, 08 Oct 2020 19:54:13: 18000000 INFO @ Thu, 08 Oct 2020 19:54:15: 14000000 INFO @ Thu, 08 Oct 2020 19:54:19: 24000000 INFO @ Thu, 08 Oct 2020 19:54:20: 19000000 INFO @ Thu, 08 Oct 2020 19:54:22: 15000000 INFO @ Thu, 08 Oct 2020 19:54:26: 25000000 INFO @ Thu, 08 Oct 2020 19:54:27: 20000000 INFO @ Thu, 08 Oct 2020 19:54:29: 16000000 INFO @ Thu, 08 Oct 2020 19:54:32: 26000000 INFO @ Thu, 08 Oct 2020 19:54:34: 21000000 INFO @ Thu, 08 Oct 2020 19:54:35: 17000000 INFO @ Thu, 08 Oct 2020 19:54:39: 27000000 INFO @ Thu, 08 Oct 2020 19:54:41: 22000000 INFO @ Thu, 08 Oct 2020 19:54:42: 18000000 INFO @ Thu, 08 Oct 2020 19:54:46: 28000000 INFO @ Thu, 08 Oct 2020 19:54:48: 23000000 INFO @ Thu, 08 Oct 2020 19:54:49: 19000000 INFO @ Thu, 08 Oct 2020 19:54:53: 29000000 INFO @ Thu, 08 Oct 2020 19:54:54: 24000000 INFO @ Thu, 08 Oct 2020 19:54:56: 20000000 INFO @ Thu, 08 Oct 2020 19:55:00: 30000000 INFO @ Thu, 08 Oct 2020 19:55:01: 25000000 INFO @ Thu, 08 Oct 2020 19:55:03: 21000000 INFO @ Thu, 08 Oct 2020 19:55:08: 31000000 INFO @ Thu, 08 Oct 2020 19:55:09: 26000000 INFO @ Thu, 08 Oct 2020 19:55:10: 22000000 INFO @ Thu, 08 Oct 2020 19:55:15: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:55:16: 27000000 INFO @ Thu, 08 Oct 2020 19:55:17: 23000000 INFO @ Thu, 08 Oct 2020 19:55:22: 33000000 INFO @ Thu, 08 Oct 2020 19:55:23: 28000000 INFO @ Thu, 08 Oct 2020 19:55:24: 24000000 INFO @ Thu, 08 Oct 2020 19:55:29: 34000000 INFO @ Thu, 08 Oct 2020 19:55:30: 29000000 INFO @ Thu, 08 Oct 2020 19:55:31: 25000000 INFO @ Thu, 08 Oct 2020 19:55:32: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:32: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:32: #1 total tags in treatment: 34299169 INFO @ Thu, 08 Oct 2020 19:55:32: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:32: #1 tags after filtering in treatment: 34299169 INFO @ Thu, 08 Oct 2020 19:55:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:32: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:32: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:34: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 19:55:34: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:38: 30000000 INFO @ Thu, 08 Oct 2020 19:55:38: 26000000 INFO @ Thu, 08 Oct 2020 19:55:45: 31000000 INFO @ Thu, 08 Oct 2020 19:55:45: 27000000 INFO @ Thu, 08 Oct 2020 19:55:52: 32000000 INFO @ Thu, 08 Oct 2020 19:55:52: 28000000 INFO @ Thu, 08 Oct 2020 19:55:59: 33000000 INFO @ Thu, 08 Oct 2020 19:55:59: 29000000 INFO @ Thu, 08 Oct 2020 19:56:06: 34000000 INFO @ Thu, 08 Oct 2020 19:56:06: 30000000 INFO @ Thu, 08 Oct 2020 19:56:08: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:08: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:08: #1 total tags in treatment: 34299169 INFO @ Thu, 08 Oct 2020 19:56:08: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:08: #1 tags after filtering in treatment: 34299169 INFO @ Thu, 08 Oct 2020 19:56:08: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:08: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:08: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:08: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:10: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 19:56:10: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:56:13: 31000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:56:19: 32000000 INFO @ Thu, 08 Oct 2020 19:56:26: 33000000 INFO @ Thu, 08 Oct 2020 19:56:32: 34000000 INFO @ Thu, 08 Oct 2020 19:56:34: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:34: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:34: #1 total tags in treatment: 34299169 INFO @ Thu, 08 Oct 2020 19:56:34: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:35: #1 tags after filtering in treatment: 34299169 INFO @ Thu, 08 Oct 2020 19:56:35: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:35: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:35: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:35: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:37: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 19:56:37: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879254/SRX7879254.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling