Job ID = 10165645 SRX = SRX7879253 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 45228743 spots for SRR11272868/SRR11272868.sra Written 45228743 spots for SRR11272868/SRR11272868.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165952 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:32 45228743 reads; of these: 45228743 (100.00%) were unpaired; of these: 542379 (1.20%) aligned 0 times 36333549 (80.33%) aligned exactly 1 time 8352815 (18.47%) aligned >1 times 98.80% overall alignment rate Time searching: 00:10:32 Overall time: 00:10:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8805492 / 44686364 = 0.1971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:53:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:53:47: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:53:47: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:53:55: 1000000 INFO @ Thu, 08 Oct 2020 19:54:02: 2000000 INFO @ Thu, 08 Oct 2020 19:54:09: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:54:17: 4000000 INFO @ Thu, 08 Oct 2020 19:54:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:54:17: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:54:17: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:54:24: 5000000 INFO @ Thu, 08 Oct 2020 19:54:25: 1000000 INFO @ Thu, 08 Oct 2020 19:54:32: 6000000 INFO @ Thu, 08 Oct 2020 19:54:33: 2000000 INFO @ Thu, 08 Oct 2020 19:54:40: 7000000 INFO @ Thu, 08 Oct 2020 19:54:42: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:54:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:54:47: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:54:47: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:54:48: 8000000 INFO @ Thu, 08 Oct 2020 19:54:50: 4000000 INFO @ Thu, 08 Oct 2020 19:54:55: 1000000 INFO @ Thu, 08 Oct 2020 19:54:56: 9000000 INFO @ Thu, 08 Oct 2020 19:54:58: 5000000 INFO @ Thu, 08 Oct 2020 19:55:04: 2000000 INFO @ Thu, 08 Oct 2020 19:55:04: 10000000 INFO @ Thu, 08 Oct 2020 19:55:06: 6000000 INFO @ Thu, 08 Oct 2020 19:55:12: 11000000 INFO @ Thu, 08 Oct 2020 19:55:12: 3000000 INFO @ Thu, 08 Oct 2020 19:55:14: 7000000 INFO @ Thu, 08 Oct 2020 19:55:19: 12000000 INFO @ Thu, 08 Oct 2020 19:55:21: 4000000 INFO @ Thu, 08 Oct 2020 19:55:22: 8000000 INFO @ Thu, 08 Oct 2020 19:55:27: 13000000 INFO @ Thu, 08 Oct 2020 19:55:29: 5000000 INFO @ Thu, 08 Oct 2020 19:55:30: 9000000 INFO @ Thu, 08 Oct 2020 19:55:35: 14000000 INFO @ Thu, 08 Oct 2020 19:55:37: 6000000 INFO @ Thu, 08 Oct 2020 19:55:38: 10000000 INFO @ Thu, 08 Oct 2020 19:55:43: 15000000 INFO @ Thu, 08 Oct 2020 19:55:45: 7000000 INFO @ Thu, 08 Oct 2020 19:55:46: 11000000 INFO @ Thu, 08 Oct 2020 19:55:50: 16000000 INFO @ Thu, 08 Oct 2020 19:55:53: 8000000 INFO @ Thu, 08 Oct 2020 19:55:54: 12000000 INFO @ Thu, 08 Oct 2020 19:55:58: 17000000 INFO @ Thu, 08 Oct 2020 19:56:01: 9000000 INFO @ Thu, 08 Oct 2020 19:56:02: 13000000 INFO @ Thu, 08 Oct 2020 19:56:06: 18000000 INFO @ Thu, 08 Oct 2020 19:56:10: 10000000 INFO @ Thu, 08 Oct 2020 19:56:11: 14000000 INFO @ Thu, 08 Oct 2020 19:56:14: 19000000 INFO @ Thu, 08 Oct 2020 19:56:18: 11000000 INFO @ Thu, 08 Oct 2020 19:56:19: 15000000 INFO @ Thu, 08 Oct 2020 19:56:21: 20000000 INFO @ Thu, 08 Oct 2020 19:56:27: 16000000 INFO @ Thu, 08 Oct 2020 19:56:27: 12000000 INFO @ Thu, 08 Oct 2020 19:56:29: 21000000 INFO @ Thu, 08 Oct 2020 19:56:35: 17000000 INFO @ Thu, 08 Oct 2020 19:56:36: 13000000 INFO @ Thu, 08 Oct 2020 19:56:36: 22000000 INFO @ Thu, 08 Oct 2020 19:56:43: 18000000 INFO @ Thu, 08 Oct 2020 19:56:44: 23000000 INFO @ Thu, 08 Oct 2020 19:56:45: 14000000 INFO @ Thu, 08 Oct 2020 19:56:50: 19000000 INFO @ Thu, 08 Oct 2020 19:56:52: 24000000 INFO @ Thu, 08 Oct 2020 19:56:53: 15000000 INFO @ Thu, 08 Oct 2020 19:56:58: 20000000 INFO @ Thu, 08 Oct 2020 19:56:59: 25000000 INFO @ Thu, 08 Oct 2020 19:57:02: 16000000 INFO @ Thu, 08 Oct 2020 19:57:06: 21000000 INFO @ Thu, 08 Oct 2020 19:57:07: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:57:10: 17000000 INFO @ Thu, 08 Oct 2020 19:57:14: 22000000 INFO @ Thu, 08 Oct 2020 19:57:14: 27000000 INFO @ Thu, 08 Oct 2020 19:57:18: 18000000 INFO @ Thu, 08 Oct 2020 19:57:21: 23000000 INFO @ Thu, 08 Oct 2020 19:57:22: 28000000 INFO @ Thu, 08 Oct 2020 19:57:26: 19000000 INFO @ Thu, 08 Oct 2020 19:57:29: 24000000 INFO @ Thu, 08 Oct 2020 19:57:30: 29000000 INFO @ Thu, 08 Oct 2020 19:57:34: 20000000 INFO @ Thu, 08 Oct 2020 19:57:37: 25000000 INFO @ Thu, 08 Oct 2020 19:57:37: 30000000 INFO @ Thu, 08 Oct 2020 19:57:42: 21000000 INFO @ Thu, 08 Oct 2020 19:57:45: 26000000 INFO @ Thu, 08 Oct 2020 19:57:45: 31000000 INFO @ Thu, 08 Oct 2020 19:57:50: 22000000 INFO @ Thu, 08 Oct 2020 19:57:52: 32000000 INFO @ Thu, 08 Oct 2020 19:57:53: 27000000 INFO @ Thu, 08 Oct 2020 19:57:58: 23000000 INFO @ Thu, 08 Oct 2020 19:58:00: 33000000 INFO @ Thu, 08 Oct 2020 19:58:01: 28000000 INFO @ Thu, 08 Oct 2020 19:58:06: 24000000 INFO @ Thu, 08 Oct 2020 19:58:08: 34000000 INFO @ Thu, 08 Oct 2020 19:58:09: 29000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:58:14: 25000000 INFO @ Thu, 08 Oct 2020 19:58:15: 35000000 INFO @ Thu, 08 Oct 2020 19:58:17: 30000000 INFO @ Thu, 08 Oct 2020 19:58:22: 26000000 INFO @ Thu, 08 Oct 2020 19:58:22: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:58:22: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:58:22: #1 total tags in treatment: 35880872 INFO @ Thu, 08 Oct 2020 19:58:22: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:58:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:58:23: #1 tags after filtering in treatment: 35880872 INFO @ Thu, 08 Oct 2020 19:58:23: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:58:23: #1 finished! INFO @ Thu, 08 Oct 2020 19:58:23: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:58:23: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:58:25: 31000000 INFO @ Thu, 08 Oct 2020 19:58:25: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 19:58:25: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:58:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:58:30: 27000000 INFO @ Thu, 08 Oct 2020 19:58:32: 32000000 INFO @ Thu, 08 Oct 2020 19:58:37: 28000000 INFO @ Thu, 08 Oct 2020 19:58:40: 33000000 INFO @ Thu, 08 Oct 2020 19:58:45: 29000000 INFO @ Thu, 08 Oct 2020 19:58:47: 34000000 INFO @ Thu, 08 Oct 2020 19:58:52: 30000000 INFO @ Thu, 08 Oct 2020 19:58:55: 35000000 INFO @ Thu, 08 Oct 2020 19:59:00: 31000000 INFO @ Thu, 08 Oct 2020 19:59:02: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:59:02: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:59:02: #1 total tags in treatment: 35880872 INFO @ Thu, 08 Oct 2020 19:59:02: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:59:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:59:02: #1 tags after filtering in treatment: 35880872 INFO @ Thu, 08 Oct 2020 19:59:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:59:02: #1 finished! INFO @ Thu, 08 Oct 2020 19:59:02: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:59:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:59:04: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 19:59:04: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:59:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:59:07: 32000000 INFO @ Thu, 08 Oct 2020 19:59:14: 33000000 INFO @ Thu, 08 Oct 2020 19:59:20: 34000000 INFO @ Thu, 08 Oct 2020 19:59:27: 35000000 INFO @ Thu, 08 Oct 2020 19:59:33: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:59:33: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:59:33: #1 total tags in treatment: 35880872 INFO @ Thu, 08 Oct 2020 19:59:33: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:59:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:59:33: #1 tags after filtering in treatment: 35880872 INFO @ Thu, 08 Oct 2020 19:59:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:59:33: #1 finished! INFO @ Thu, 08 Oct 2020 19:59:33: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:59:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:59:35: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 19:59:35: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:59:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879253/SRX7879253.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling