Job ID = 10165643 SRX = SRX7879251 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 41940504 spots for SRR11272866/SRR11272866.sra Written 41940504 spots for SRR11272866/SRR11272866.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165940 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:36 41940504 reads; of these: 41940504 (100.00%) were unpaired; of these: 612676 (1.46%) aligned 0 times 33842688 (80.69%) aligned exactly 1 time 7485140 (17.85%) aligned >1 times 98.54% overall alignment rate Time searching: 00:09:36 Overall time: 00:09:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7441533 / 41327828 = 0.1801 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:58: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:58: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:04: 1000000 INFO @ Thu, 08 Oct 2020 19:52:09: 2000000 INFO @ Thu, 08 Oct 2020 19:52:15: 3000000 INFO @ Thu, 08 Oct 2020 19:52:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:26: 5000000 INFO @ Thu, 08 Oct 2020 19:52:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:28: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:28: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:33: 6000000 INFO @ Thu, 08 Oct 2020 19:52:35: 1000000 INFO @ Thu, 08 Oct 2020 19:52:39: 7000000 INFO @ Thu, 08 Oct 2020 19:52:41: 2000000 INFO @ Thu, 08 Oct 2020 19:52:46: 8000000 INFO @ Thu, 08 Oct 2020 19:52:48: 3000000 INFO @ Thu, 08 Oct 2020 19:52:52: 9000000 INFO @ Thu, 08 Oct 2020 19:52:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:58: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:58: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:58: 10000000 INFO @ Thu, 08 Oct 2020 19:53:01: 5000000 INFO @ Thu, 08 Oct 2020 19:53:04: 1000000 INFO @ Thu, 08 Oct 2020 19:53:05: 11000000 INFO @ Thu, 08 Oct 2020 19:53:08: 6000000 INFO @ Thu, 08 Oct 2020 19:53:11: 2000000 INFO @ Thu, 08 Oct 2020 19:53:11: 12000000 INFO @ Thu, 08 Oct 2020 19:53:14: 7000000 INFO @ Thu, 08 Oct 2020 19:53:18: 3000000 INFO @ Thu, 08 Oct 2020 19:53:18: 13000000 INFO @ Thu, 08 Oct 2020 19:53:21: 8000000 INFO @ Thu, 08 Oct 2020 19:53:24: 14000000 INFO @ Thu, 08 Oct 2020 19:53:24: 4000000 INFO @ Thu, 08 Oct 2020 19:53:27: 9000000 INFO @ Thu, 08 Oct 2020 19:53:30: 15000000 INFO @ Thu, 08 Oct 2020 19:53:30: 5000000 INFO @ Thu, 08 Oct 2020 19:53:34: 10000000 INFO @ Thu, 08 Oct 2020 19:53:36: 16000000 INFO @ Thu, 08 Oct 2020 19:53:37: 6000000 INFO @ Thu, 08 Oct 2020 19:53:41: 11000000 INFO @ Thu, 08 Oct 2020 19:53:43: 17000000 INFO @ Thu, 08 Oct 2020 19:53:43: 7000000 INFO @ Thu, 08 Oct 2020 19:53:47: 12000000 INFO @ Thu, 08 Oct 2020 19:53:49: 18000000 INFO @ Thu, 08 Oct 2020 19:53:50: 8000000 INFO @ Thu, 08 Oct 2020 19:53:54: 13000000 INFO @ Thu, 08 Oct 2020 19:53:56: 19000000 INFO @ Thu, 08 Oct 2020 19:53:56: 9000000 INFO @ Thu, 08 Oct 2020 19:54:00: 14000000 INFO @ Thu, 08 Oct 2020 19:54:02: 20000000 INFO @ Thu, 08 Oct 2020 19:54:03: 10000000 INFO @ Thu, 08 Oct 2020 19:54:07: 15000000 INFO @ Thu, 08 Oct 2020 19:54:08: 21000000 INFO @ Thu, 08 Oct 2020 19:54:09: 11000000 INFO @ Thu, 08 Oct 2020 19:54:13: 16000000 INFO @ Thu, 08 Oct 2020 19:54:15: 22000000 INFO @ Thu, 08 Oct 2020 19:54:16: 12000000 INFO @ Thu, 08 Oct 2020 19:54:19: 17000000 INFO @ Thu, 08 Oct 2020 19:54:21: 23000000 INFO @ Thu, 08 Oct 2020 19:54:22: 13000000 INFO @ Thu, 08 Oct 2020 19:54:26: 18000000 INFO @ Thu, 08 Oct 2020 19:54:28: 24000000 INFO @ Thu, 08 Oct 2020 19:54:28: 14000000 INFO @ Thu, 08 Oct 2020 19:54:33: 19000000 INFO @ Thu, 08 Oct 2020 19:54:34: 25000000 INFO @ Thu, 08 Oct 2020 19:54:35: 15000000 INFO @ Thu, 08 Oct 2020 19:54:39: 20000000 INFO @ Thu, 08 Oct 2020 19:54:40: 26000000 INFO @ Thu, 08 Oct 2020 19:54:41: 16000000 INFO @ Thu, 08 Oct 2020 19:54:46: 21000000 INFO @ Thu, 08 Oct 2020 19:54:46: 27000000 INFO @ Thu, 08 Oct 2020 19:54:48: 17000000 INFO @ Thu, 08 Oct 2020 19:54:52: 22000000 INFO @ Thu, 08 Oct 2020 19:54:53: 28000000 INFO @ Thu, 08 Oct 2020 19:54:54: 18000000 INFO @ Thu, 08 Oct 2020 19:54:59: 23000000 INFO @ Thu, 08 Oct 2020 19:54:59: 29000000 INFO @ Thu, 08 Oct 2020 19:55:00: 19000000 INFO @ Thu, 08 Oct 2020 19:55:05: 24000000 INFO @ Thu, 08 Oct 2020 19:55:05: 30000000 INFO @ Thu, 08 Oct 2020 19:55:07: 20000000 INFO @ Thu, 08 Oct 2020 19:55:12: 25000000 INFO @ Thu, 08 Oct 2020 19:55:12: 31000000 INFO @ Thu, 08 Oct 2020 19:55:13: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:55:18: 26000000 INFO @ Thu, 08 Oct 2020 19:55:18: 32000000 INFO @ Thu, 08 Oct 2020 19:55:20: 22000000 INFO @ Thu, 08 Oct 2020 19:55:25: 27000000 INFO @ Thu, 08 Oct 2020 19:55:25: 33000000 INFO @ Thu, 08 Oct 2020 19:55:26: 23000000 INFO @ Thu, 08 Oct 2020 19:55:31: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:31: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:31: #1 total tags in treatment: 33886295 INFO @ Thu, 08 Oct 2020 19:55:31: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:31: 28000000 INFO @ Thu, 08 Oct 2020 19:55:31: #1 tags after filtering in treatment: 33886295 INFO @ Thu, 08 Oct 2020 19:55:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:31: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:31: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:32: 24000000 INFO @ Thu, 08 Oct 2020 19:55:33: #2 number of paired peaks: 33 WARNING @ Thu, 08 Oct 2020 19:55:33: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:37: 29000000 INFO @ Thu, 08 Oct 2020 19:55:39: 25000000 INFO @ Thu, 08 Oct 2020 19:55:43: 30000000 INFO @ Thu, 08 Oct 2020 19:55:45: 26000000 INFO @ Thu, 08 Oct 2020 19:55:50: 31000000 INFO @ Thu, 08 Oct 2020 19:55:51: 27000000 INFO @ Thu, 08 Oct 2020 19:55:56: 32000000 INFO @ Thu, 08 Oct 2020 19:55:57: 28000000 INFO @ Thu, 08 Oct 2020 19:56:02: 33000000 INFO @ Thu, 08 Oct 2020 19:56:03: 29000000 INFO @ Thu, 08 Oct 2020 19:56:08: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:08: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:08: #1 total tags in treatment: 33886295 INFO @ Thu, 08 Oct 2020 19:56:08: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:08: #1 tags after filtering in treatment: 33886295 INFO @ Thu, 08 Oct 2020 19:56:08: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:08: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:08: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:08: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:09: 30000000 INFO @ Thu, 08 Oct 2020 19:56:10: #2 number of paired peaks: 33 WARNING @ Thu, 08 Oct 2020 19:56:10: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:56:15: 31000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:56:21: 32000000 INFO @ Thu, 08 Oct 2020 19:56:26: 33000000 INFO @ Thu, 08 Oct 2020 19:56:32: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:32: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:32: #1 total tags in treatment: 33886295 INFO @ Thu, 08 Oct 2020 19:56:32: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:32: #1 tags after filtering in treatment: 33886295 INFO @ Thu, 08 Oct 2020 19:56:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:32: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:32: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:34: #2 number of paired peaks: 33 WARNING @ Thu, 08 Oct 2020 19:56:34: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling