Job ID = 10165640 SRX = SRX7879248 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 43398663 spots for SRR11272863/SRR11272863.sra Written 43398663 spots for SRR11272863/SRR11272863.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165942 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:50 43398663 reads; of these: 43398663 (100.00%) were unpaired; of these: 700587 (1.61%) aligned 0 times 35754396 (82.39%) aligned exactly 1 time 6943680 (16.00%) aligned >1 times 98.39% overall alignment rate Time searching: 00:09:50 Overall time: 00:09:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7318541 / 42698076 = 0.1714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:06: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:06: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:11: 1000000 INFO @ Thu, 08 Oct 2020 19:52:16: 2000000 INFO @ Thu, 08 Oct 2020 19:52:21: 3000000 INFO @ Thu, 08 Oct 2020 19:52:26: 4000000 INFO @ Thu, 08 Oct 2020 19:52:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:36: 6000000 INFO @ Thu, 08 Oct 2020 19:52:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:36: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:36: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:41: 7000000 INFO @ Thu, 08 Oct 2020 19:52:42: 1000000 INFO @ Thu, 08 Oct 2020 19:52:46: 8000000 INFO @ Thu, 08 Oct 2020 19:52:47: 2000000 INFO @ Thu, 08 Oct 2020 19:52:52: 9000000 INFO @ Thu, 08 Oct 2020 19:52:52: 3000000 INFO @ Thu, 08 Oct 2020 19:52:57: 10000000 INFO @ Thu, 08 Oct 2020 19:52:58: 4000000 INFO @ Thu, 08 Oct 2020 19:53:02: 11000000 INFO @ Thu, 08 Oct 2020 19:53:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:53:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:53:06: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:53:06: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:53:08: 12000000 INFO @ Thu, 08 Oct 2020 19:53:09: 6000000 INFO @ Thu, 08 Oct 2020 19:53:13: 1000000 INFO @ Thu, 08 Oct 2020 19:53:13: 13000000 INFO @ Thu, 08 Oct 2020 19:53:15: 7000000 INFO @ Thu, 08 Oct 2020 19:53:19: 14000000 INFO @ Thu, 08 Oct 2020 19:53:19: 2000000 INFO @ Thu, 08 Oct 2020 19:53:20: 8000000 INFO @ Thu, 08 Oct 2020 19:53:25: 15000000 INFO @ Thu, 08 Oct 2020 19:53:26: 3000000 INFO @ Thu, 08 Oct 2020 19:53:26: 9000000 INFO @ Thu, 08 Oct 2020 19:53:30: 16000000 INFO @ Thu, 08 Oct 2020 19:53:32: 10000000 INFO @ Thu, 08 Oct 2020 19:53:32: 4000000 INFO @ Thu, 08 Oct 2020 19:53:36: 17000000 INFO @ Thu, 08 Oct 2020 19:53:37: 11000000 INFO @ Thu, 08 Oct 2020 19:53:39: 5000000 INFO @ Thu, 08 Oct 2020 19:53:41: 18000000 INFO @ Thu, 08 Oct 2020 19:53:43: 12000000 INFO @ Thu, 08 Oct 2020 19:53:45: 6000000 INFO @ Thu, 08 Oct 2020 19:53:47: 19000000 INFO @ Thu, 08 Oct 2020 19:53:49: 13000000 INFO @ Thu, 08 Oct 2020 19:53:52: 7000000 INFO @ Thu, 08 Oct 2020 19:53:52: 20000000 INFO @ Thu, 08 Oct 2020 19:53:54: 14000000 INFO @ Thu, 08 Oct 2020 19:53:58: 21000000 INFO @ Thu, 08 Oct 2020 19:53:58: 8000000 INFO @ Thu, 08 Oct 2020 19:54:00: 15000000 INFO @ Thu, 08 Oct 2020 19:54:04: 22000000 INFO @ Thu, 08 Oct 2020 19:54:05: 9000000 INFO @ Thu, 08 Oct 2020 19:54:06: 16000000 INFO @ Thu, 08 Oct 2020 19:54:09: 23000000 INFO @ Thu, 08 Oct 2020 19:54:11: 10000000 INFO @ Thu, 08 Oct 2020 19:54:11: 17000000 INFO @ Thu, 08 Oct 2020 19:54:15: 24000000 INFO @ Thu, 08 Oct 2020 19:54:17: 18000000 INFO @ Thu, 08 Oct 2020 19:54:18: 11000000 INFO @ Thu, 08 Oct 2020 19:54:20: 25000000 INFO @ Thu, 08 Oct 2020 19:54:23: 19000000 INFO @ Thu, 08 Oct 2020 19:54:24: 12000000 INFO @ Thu, 08 Oct 2020 19:54:26: 26000000 INFO @ Thu, 08 Oct 2020 19:54:28: 20000000 INFO @ Thu, 08 Oct 2020 19:54:31: 13000000 INFO @ Thu, 08 Oct 2020 19:54:32: 27000000 INFO @ Thu, 08 Oct 2020 19:54:34: 21000000 INFO @ Thu, 08 Oct 2020 19:54:37: 14000000 INFO @ Thu, 08 Oct 2020 19:54:37: 28000000 INFO @ Thu, 08 Oct 2020 19:54:39: 22000000 INFO @ Thu, 08 Oct 2020 19:54:43: 29000000 INFO @ Thu, 08 Oct 2020 19:54:44: 15000000 INFO @ Thu, 08 Oct 2020 19:54:45: 23000000 INFO @ Thu, 08 Oct 2020 19:54:48: 30000000 INFO @ Thu, 08 Oct 2020 19:54:50: 16000000 INFO @ Thu, 08 Oct 2020 19:54:51: 24000000 INFO @ Thu, 08 Oct 2020 19:54:54: 31000000 INFO @ Thu, 08 Oct 2020 19:54:56: 25000000 INFO @ Thu, 08 Oct 2020 19:54:56: 17000000 INFO @ Thu, 08 Oct 2020 19:55:00: 32000000 INFO @ Thu, 08 Oct 2020 19:55:02: 26000000 INFO @ Thu, 08 Oct 2020 19:55:03: 18000000 INFO @ Thu, 08 Oct 2020 19:55:05: 33000000 INFO @ Thu, 08 Oct 2020 19:55:08: 27000000 INFO @ Thu, 08 Oct 2020 19:55:09: 19000000 INFO @ Thu, 08 Oct 2020 19:55:11: 34000000 INFO @ Thu, 08 Oct 2020 19:55:13: 28000000 INFO @ Thu, 08 Oct 2020 19:55:16: 20000000 INFO @ Thu, 08 Oct 2020 19:55:16: 35000000 INFO @ Thu, 08 Oct 2020 19:55:19: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:19: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:19: #1 total tags in treatment: 35379535 INFO @ Thu, 08 Oct 2020 19:55:19: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:19: 29000000 INFO @ Thu, 08 Oct 2020 19:55:19: #1 tags after filtering in treatment: 35379535 INFO @ Thu, 08 Oct 2020 19:55:19: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:19: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:19: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:19: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:21: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 19:55:21: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:22: 21000000 INFO @ Thu, 08 Oct 2020 19:55:24: 30000000 INFO @ Thu, 08 Oct 2020 19:55:28: 22000000 INFO @ Thu, 08 Oct 2020 19:55:30: 31000000 INFO @ Thu, 08 Oct 2020 19:55:35: 23000000 INFO @ Thu, 08 Oct 2020 19:55:36: 32000000 INFO @ Thu, 08 Oct 2020 19:55:41: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:55:41: 33000000 INFO @ Thu, 08 Oct 2020 19:55:47: 34000000 INFO @ Thu, 08 Oct 2020 19:55:48: 25000000 INFO @ Thu, 08 Oct 2020 19:55:53: 35000000 INFO @ Thu, 08 Oct 2020 19:55:54: 26000000 INFO @ Thu, 08 Oct 2020 19:55:55: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:55: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:55: #1 total tags in treatment: 35379535 INFO @ Thu, 08 Oct 2020 19:55:55: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:56: #1 tags after filtering in treatment: 35379535 INFO @ Thu, 08 Oct 2020 19:55:56: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:56: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:56: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:56: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:58: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 19:55:58: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:56:00: 27000000 INFO @ Thu, 08 Oct 2020 19:56:07: 28000000 INFO @ Thu, 08 Oct 2020 19:56:13: 29000000 INFO @ Thu, 08 Oct 2020 19:56:19: 30000000 INFO @ Thu, 08 Oct 2020 19:56:25: 31000000 INFO @ Thu, 08 Oct 2020 19:56:32: 32000000 INFO @ Thu, 08 Oct 2020 19:56:38: 33000000 INFO @ Thu, 08 Oct 2020 19:56:44: 34000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:56:50: 35000000 INFO @ Thu, 08 Oct 2020 19:56:53: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:53: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:53: #1 total tags in treatment: 35379535 INFO @ Thu, 08 Oct 2020 19:56:53: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:53: #1 tags after filtering in treatment: 35379535 INFO @ Thu, 08 Oct 2020 19:56:53: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:53: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:53: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:53: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:55: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 19:56:55: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling