Job ID = 6626385 SRX = SRX7262222 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8270184 spots for SRR10581845/SRR10581845.sra Written 8270184 spots for SRR10581845/SRR10581845.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626470 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:45 8270184 reads; of these: 8270184 (100.00%) were unpaired; of these: 2059628 (24.90%) aligned 0 times 4445167 (53.75%) aligned exactly 1 time 1765389 (21.35%) aligned >1 times 75.10% overall alignment rate Time searching: 00:01:45 Overall time: 00:01:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1309939 / 6210556 = 0.2109 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:01:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:01:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:01:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:01:17: 1000000 INFO @ Tue, 14 Jul 2020 07:01:23: 2000000 INFO @ Tue, 14 Jul 2020 07:01:28: 3000000 INFO @ Tue, 14 Jul 2020 07:01:33: 4000000 INFO @ Tue, 14 Jul 2020 07:01:38: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:01:38: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:01:38: #1 total tags in treatment: 4900617 INFO @ Tue, 14 Jul 2020 07:01:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:01:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:01:38: #1 tags after filtering in treatment: 4900617 INFO @ Tue, 14 Jul 2020 07:01:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:01:38: #1 finished! INFO @ Tue, 14 Jul 2020 07:01:38: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:01:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:01:38: #2 number of paired peaks: 2803 INFO @ Tue, 14 Jul 2020 07:01:38: start model_add_line... INFO @ Tue, 14 Jul 2020 07:01:38: start X-correlation... INFO @ Tue, 14 Jul 2020 07:01:38: end of X-cor INFO @ Tue, 14 Jul 2020 07:01:38: #2 finished! INFO @ Tue, 14 Jul 2020 07:01:38: #2 predicted fragment length is 154 bps INFO @ Tue, 14 Jul 2020 07:01:38: #2 alternative fragment length(s) may be 3,134,154 bps INFO @ Tue, 14 Jul 2020 07:01:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.05_model.r INFO @ Tue, 14 Jul 2020 07:01:38: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:01:38: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:01:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:01:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:01:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:01:48: 1000000 INFO @ Tue, 14 Jul 2020 07:01:51: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:01:54: 2000000 INFO @ Tue, 14 Jul 2020 07:01:56: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.05_peaks.xls INFO @ Tue, 14 Jul 2020 07:01:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:01:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.05_summits.bed INFO @ Tue, 14 Jul 2020 07:01:56: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1339 records, 4 fields): 24 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:02:00: 3000000 INFO @ Tue, 14 Jul 2020 07:02:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:02:11: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:02:11: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:02:11: #1 total tags in treatment: 4900617 INFO @ Tue, 14 Jul 2020 07:02:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:02:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:02:11: #1 tags after filtering in treatment: 4900617 INFO @ Tue, 14 Jul 2020 07:02:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:02:11: #1 finished! INFO @ Tue, 14 Jul 2020 07:02:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:02:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:02:12: #2 number of paired peaks: 2803 INFO @ Tue, 14 Jul 2020 07:02:12: start model_add_line... INFO @ Tue, 14 Jul 2020 07:02:12: start X-correlation... INFO @ Tue, 14 Jul 2020 07:02:12: end of X-cor INFO @ Tue, 14 Jul 2020 07:02:12: #2 finished! INFO @ Tue, 14 Jul 2020 07:02:12: #2 predicted fragment length is 154 bps INFO @ Tue, 14 Jul 2020 07:02:12: #2 alternative fragment length(s) may be 3,134,154 bps INFO @ Tue, 14 Jul 2020 07:02:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.10_model.r INFO @ Tue, 14 Jul 2020 07:02:12: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:02:12: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:02:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:02:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:02:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:02:18: 1000000 INFO @ Tue, 14 Jul 2020 07:02:24: 2000000 INFO @ Tue, 14 Jul 2020 07:02:24: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:02:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.10_peaks.xls INFO @ Tue, 14 Jul 2020 07:02:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:02:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.10_summits.bed INFO @ Tue, 14 Jul 2020 07:02:30: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (441 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:02:30: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:02:36: 4000000 INFO @ Tue, 14 Jul 2020 07:02:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:02:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:02:41: #1 total tags in treatment: 4900617 INFO @ Tue, 14 Jul 2020 07:02:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:02:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:02:41: #1 tags after filtering in treatment: 4900617 INFO @ Tue, 14 Jul 2020 07:02:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:02:41: #1 finished! INFO @ Tue, 14 Jul 2020 07:02:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:02:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:02:42: #2 number of paired peaks: 2803 INFO @ Tue, 14 Jul 2020 07:02:42: start model_add_line... INFO @ Tue, 14 Jul 2020 07:02:42: start X-correlation... INFO @ Tue, 14 Jul 2020 07:02:42: end of X-cor INFO @ Tue, 14 Jul 2020 07:02:42: #2 finished! INFO @ Tue, 14 Jul 2020 07:02:42: #2 predicted fragment length is 154 bps INFO @ Tue, 14 Jul 2020 07:02:42: #2 alternative fragment length(s) may be 3,134,154 bps INFO @ Tue, 14 Jul 2020 07:02:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.20_model.r INFO @ Tue, 14 Jul 2020 07:02:42: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:02:42: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:02:54: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:02:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.20_peaks.xls INFO @ Tue, 14 Jul 2020 07:02:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:02:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262222/SRX7262222.20_summits.bed INFO @ Tue, 14 Jul 2020 07:02:59: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (188 records, 4 fields): 16 millis CompletedMACS2peakCalling