Job ID = 6626356 SRX = SRX7262196 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 1872207 spots for SRR10581819/SRR10581819.sra Written 1872207 spots for SRR10581819/SRR10581819.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626373 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:25 1872207 reads; of these: 1872207 (100.00%) were unpaired; of these: 611619 (32.67%) aligned 0 times 958653 (51.20%) aligned exactly 1 time 301935 (16.13%) aligned >1 times 67.33% overall alignment rate Time searching: 00:00:25 Overall time: 00:00:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 212835 / 1260588 = 0.1688 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:56:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:56:59: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:56:59: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:57:05: 1000000 INFO @ Tue, 14 Jul 2020 06:57:05: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:57:05: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:57:05: #1 total tags in treatment: 1047753 INFO @ Tue, 14 Jul 2020 06:57:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:57:05: #1 tags after filtering in treatment: 1047753 INFO @ Tue, 14 Jul 2020 06:57:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:57:05: #1 finished! INFO @ Tue, 14 Jul 2020 06:57:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:57:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:57:05: #2 number of paired peaks: 427 WARNING @ Tue, 14 Jul 2020 06:57:05: Fewer paired peaks (427) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 427 pairs to build model! INFO @ Tue, 14 Jul 2020 06:57:05: start model_add_line... INFO @ Tue, 14 Jul 2020 06:57:05: start X-correlation... INFO @ Tue, 14 Jul 2020 06:57:06: end of X-cor INFO @ Tue, 14 Jul 2020 06:57:06: #2 finished! INFO @ Tue, 14 Jul 2020 06:57:06: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:57:06: #2 alternative fragment length(s) may be 48,140,237,423,506 bps INFO @ Tue, 14 Jul 2020 06:57:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.05_model.r WARNING @ Tue, 14 Jul 2020 06:57:06: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:57:06: #2 You may need to consider one of the other alternative d(s): 48,140,237,423,506 WARNING @ Tue, 14 Jul 2020 06:57:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:57:06: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:57:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 06:57:08: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 06:57:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.05_peaks.xls INFO @ Tue, 14 Jul 2020 06:57:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 06:57:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.05_summits.bed INFO @ Tue, 14 Jul 2020 06:57:09: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (210 records, 4 fields): 20 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:57:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:57:28: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:57:28: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:57:33: 1000000 INFO @ Tue, 14 Jul 2020 06:57:34: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:57:34: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:57:34: #1 total tags in treatment: 1047753 INFO @ Tue, 14 Jul 2020 06:57:34: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:57:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:57:34: #1 tags after filtering in treatment: 1047753 INFO @ Tue, 14 Jul 2020 06:57:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:57:34: #1 finished! INFO @ Tue, 14 Jul 2020 06:57:34: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:57:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:57:34: #2 number of paired peaks: 427 WARNING @ Tue, 14 Jul 2020 06:57:34: Fewer paired peaks (427) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 427 pairs to build model! INFO @ Tue, 14 Jul 2020 06:57:34: start model_add_line... INFO @ Tue, 14 Jul 2020 06:57:34: start X-correlation... INFO @ Tue, 14 Jul 2020 06:57:34: end of X-cor INFO @ Tue, 14 Jul 2020 06:57:34: #2 finished! INFO @ Tue, 14 Jul 2020 06:57:34: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:57:34: #2 alternative fragment length(s) may be 48,140,237,423,506 bps INFO @ Tue, 14 Jul 2020 06:57:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.10_model.r WARNING @ Tue, 14 Jul 2020 06:57:34: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:57:34: #2 You may need to consider one of the other alternative d(s): 48,140,237,423,506 WARNING @ Tue, 14 Jul 2020 06:57:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:57:34: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:57:34: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 06:57:36: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 06:57:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.10_peaks.xls INFO @ Tue, 14 Jul 2020 06:57:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 06:57:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.10_summits.bed INFO @ Tue, 14 Jul 2020 06:57:38: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (132 records, 4 fields): 24 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:57:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:57:58: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:57:58: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 06:58:04: 1000000 INFO @ Tue, 14 Jul 2020 06:58:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:58:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:58:04: #1 total tags in treatment: 1047753 INFO @ Tue, 14 Jul 2020 06:58:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:58:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:58:04: #1 tags after filtering in treatment: 1047753 INFO @ Tue, 14 Jul 2020 06:58:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:58:04: #1 finished! INFO @ Tue, 14 Jul 2020 06:58:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:58:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:58:04: #2 number of paired peaks: 427 WARNING @ Tue, 14 Jul 2020 06:58:04: Fewer paired peaks (427) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 427 pairs to build model! INFO @ Tue, 14 Jul 2020 06:58:04: start model_add_line... INFO @ Tue, 14 Jul 2020 06:58:04: start X-correlation... INFO @ Tue, 14 Jul 2020 06:58:04: end of X-cor INFO @ Tue, 14 Jul 2020 06:58:04: #2 finished! INFO @ Tue, 14 Jul 2020 06:58:04: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:58:04: #2 alternative fragment length(s) may be 48,140,237,423,506 bps INFO @ Tue, 14 Jul 2020 06:58:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.20_model.r WARNING @ Tue, 14 Jul 2020 06:58:04: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:58:04: #2 You may need to consider one of the other alternative d(s): 48,140,237,423,506 WARNING @ Tue, 14 Jul 2020 06:58:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:58:04: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:58:04: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 06:58:07: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 06:58:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.20_peaks.xls INFO @ Tue, 14 Jul 2020 06:58:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 06:58:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262196/SRX7262196.20_summits.bed INFO @ Tue, 14 Jul 2020 06:58:08: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (62 records, 4 fields): 25 millis CompletedMACS2peakCalling