Job ID = 12264819 SRX = SRX7246276 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7783246 spots for SRR10564693/SRR10564693.sra Written 7783246 spots for SRR10564693/SRR10564693.sra Read 7833114 spots for SRR10564694/SRR10564694.sra Written 7833114 spots for SRR10564694/SRR10564694.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265025 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:08 15616360 reads; of these: 15616360 (100.00%) were unpaired; of these: 978148 (6.26%) aligned 0 times 12849290 (82.28%) aligned exactly 1 time 1788922 (11.46%) aligned >1 times 93.74% overall alignment rate Time searching: 00:03:08 Overall time: 00:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9669535 / 14638212 = 0.6606 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:00:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:00:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:00:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:00:56: 1000000 INFO @ Sat, 03 Apr 2021 06:01:03: 2000000 INFO @ Sat, 03 Apr 2021 06:01:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:01:17: 4000000 INFO @ Sat, 03 Apr 2021 06:01:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:01:18: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:01:18: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:01:24: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 06:01:24: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 06:01:24: #1 total tags in treatment: 4968677 INFO @ Sat, 03 Apr 2021 06:01:24: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:01:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:01:24: #1 tags after filtering in treatment: 4968677 INFO @ Sat, 03 Apr 2021 06:01:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:01:24: #1 finished! INFO @ Sat, 03 Apr 2021 06:01:24: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:01:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:01:25: 1000000 INFO @ Sat, 03 Apr 2021 06:01:25: #2 number of paired peaks: 4172 INFO @ Sat, 03 Apr 2021 06:01:25: start model_add_line... INFO @ Sat, 03 Apr 2021 06:01:25: start X-correlation... INFO @ Sat, 03 Apr 2021 06:01:25: end of X-cor INFO @ Sat, 03 Apr 2021 06:01:25: #2 finished! INFO @ Sat, 03 Apr 2021 06:01:25: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 06:01:25: #2 alternative fragment length(s) may be 84 bps INFO @ Sat, 03 Apr 2021 06:01:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.05_model.r WARNING @ Sat, 03 Apr 2021 06:01:25: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:01:25: #2 You may need to consider one of the other alternative d(s): 84 WARNING @ Sat, 03 Apr 2021 06:01:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:01:25: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:01:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:01:31: 2000000 INFO @ Sat, 03 Apr 2021 06:01:36: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:01:37: 3000000 INFO @ Sat, 03 Apr 2021 06:01:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:01:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:01:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.05_summits.bed INFO @ Sat, 03 Apr 2021 06:01:43: Done! INFO @ Sat, 03 Apr 2021 06:01:43: 4000000 pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10462 records, 4 fields): 15 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:01:49: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 06:01:49: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 06:01:49: #1 total tags in treatment: 4968677 INFO @ Sat, 03 Apr 2021 06:01:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:01:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:01:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:01:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:01:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:01:49: #1 tags after filtering in treatment: 4968677 INFO @ Sat, 03 Apr 2021 06:01:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:01:49: #1 finished! INFO @ Sat, 03 Apr 2021 06:01:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:01:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:01:49: #2 number of paired peaks: 4172 INFO @ Sat, 03 Apr 2021 06:01:49: start model_add_line... INFO @ Sat, 03 Apr 2021 06:01:49: start X-correlation... INFO @ Sat, 03 Apr 2021 06:01:49: end of X-cor INFO @ Sat, 03 Apr 2021 06:01:49: #2 finished! INFO @ Sat, 03 Apr 2021 06:01:49: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 06:01:49: #2 alternative fragment length(s) may be 84 bps INFO @ Sat, 03 Apr 2021 06:01:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.10_model.r WARNING @ Sat, 03 Apr 2021 06:01:49: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:01:49: #2 You may need to consider one of the other alternative d(s): 84 WARNING @ Sat, 03 Apr 2021 06:01:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:01:49: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:01:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:01:56: 1000000 INFO @ Sat, 03 Apr 2021 06:02:00: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:02:03: 2000000 INFO @ Sat, 03 Apr 2021 06:02:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:02:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:02:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.10_summits.bed INFO @ Sat, 03 Apr 2021 06:02:06: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7916 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:02:10: 3000000 INFO @ Sat, 03 Apr 2021 06:02:17: 4000000 INFO @ Sat, 03 Apr 2021 06:02:24: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 06:02:24: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 06:02:24: #1 total tags in treatment: 4968677 INFO @ Sat, 03 Apr 2021 06:02:24: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:02:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:02:24: #1 tags after filtering in treatment: 4968677 INFO @ Sat, 03 Apr 2021 06:02:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:02:24: #1 finished! INFO @ Sat, 03 Apr 2021 06:02:24: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:02:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:02:25: #2 number of paired peaks: 4172 INFO @ Sat, 03 Apr 2021 06:02:25: start model_add_line... INFO @ Sat, 03 Apr 2021 06:02:25: start X-correlation... INFO @ Sat, 03 Apr 2021 06:02:25: end of X-cor INFO @ Sat, 03 Apr 2021 06:02:25: #2 finished! INFO @ Sat, 03 Apr 2021 06:02:25: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 06:02:25: #2 alternative fragment length(s) may be 84 bps INFO @ Sat, 03 Apr 2021 06:02:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.20_model.r WARNING @ Sat, 03 Apr 2021 06:02:25: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:02:25: #2 You may need to consider one of the other alternative d(s): 84 WARNING @ Sat, 03 Apr 2021 06:02:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:02:25: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:02:25: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:02:36: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:02:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:02:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:02:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246276/SRX7246276.20_summits.bed INFO @ Sat, 03 Apr 2021 06:02:42: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (5013 records, 4 fields): 8 millis CompletedMACS2peakCalling