Job ID = 12264818 SRX = SRX7246275 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 1455292 spots for SRR10564691/SRR10564691.sra Written 1455292 spots for SRR10564691/SRR10564691.sra Read 1500678 spots for SRR10564692/SRR10564692.sra Written 1500678 spots for SRR10564692/SRR10564692.sra fastq に変換しました。 bowtie でマッピング中... Your job 12264881 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:38 2955970 reads; of these: 2955970 (100.00%) were unpaired; of these: 472875 (16.00%) aligned 0 times 2207894 (74.69%) aligned exactly 1 time 275201 (9.31%) aligned >1 times 84.00% overall alignment rate Time searching: 00:00:38 Overall time: 00:00:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 895100 / 2483095 = 0.3605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 05:54:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:54:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:54:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 05:54:55: 1000000 INFO @ Sat, 03 Apr 2021 05:54:59: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 05:54:59: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 05:54:59: #1 total tags in treatment: 1587995 INFO @ Sat, 03 Apr 2021 05:54:59: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 05:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 05:54:59: #1 tags after filtering in treatment: 1587995 INFO @ Sat, 03 Apr 2021 05:54:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 05:54:59: #1 finished! INFO @ Sat, 03 Apr 2021 05:54:59: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 05:54:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 05:54:59: #2 number of paired peaks: 4449 INFO @ Sat, 03 Apr 2021 05:54:59: start model_add_line... INFO @ Sat, 03 Apr 2021 05:54:59: start X-correlation... INFO @ Sat, 03 Apr 2021 05:54:59: end of X-cor INFO @ Sat, 03 Apr 2021 05:54:59: #2 finished! INFO @ Sat, 03 Apr 2021 05:54:59: #2 predicted fragment length is 75 bps INFO @ Sat, 03 Apr 2021 05:54:59: #2 alternative fragment length(s) may be 75 bps INFO @ Sat, 03 Apr 2021 05:54:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.05_model.r WARNING @ Sat, 03 Apr 2021 05:54:59: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 05:54:59: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sat, 03 Apr 2021 05:54:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 05:54:59: #3 Call peaks... INFO @ Sat, 03 Apr 2021 05:54:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 05:55:02: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 05:55:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.05_peaks.xls INFO @ Sat, 03 Apr 2021 05:55:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 05:55:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.05_summits.bed INFO @ Sat, 03 Apr 2021 05:55:04: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6203 records, 4 fields): 12 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 05:55:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:55:17: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:55:17: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 05:55:23: 1000000 INFO @ Sat, 03 Apr 2021 05:55:27: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 05:55:27: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 05:55:27: #1 total tags in treatment: 1587995 INFO @ Sat, 03 Apr 2021 05:55:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 05:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 05:55:27: #1 tags after filtering in treatment: 1587995 INFO @ Sat, 03 Apr 2021 05:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 05:55:27: #1 finished! INFO @ Sat, 03 Apr 2021 05:55:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 05:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 05:55:27: #2 number of paired peaks: 4449 INFO @ Sat, 03 Apr 2021 05:55:27: start model_add_line... INFO @ Sat, 03 Apr 2021 05:55:27: start X-correlation... INFO @ Sat, 03 Apr 2021 05:55:27: end of X-cor INFO @ Sat, 03 Apr 2021 05:55:27: #2 finished! INFO @ Sat, 03 Apr 2021 05:55:27: #2 predicted fragment length is 75 bps INFO @ Sat, 03 Apr 2021 05:55:27: #2 alternative fragment length(s) may be 75 bps INFO @ Sat, 03 Apr 2021 05:55:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.10_model.r WARNING @ Sat, 03 Apr 2021 05:55:27: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 05:55:27: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sat, 03 Apr 2021 05:55:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 05:55:27: #3 Call peaks... INFO @ Sat, 03 Apr 2021 05:55:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 05:55:30: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 05:55:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.10_peaks.xls INFO @ Sat, 03 Apr 2021 05:55:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 05:55:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.10_summits.bed INFO @ Sat, 03 Apr 2021 05:55:32: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (4226 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 05:55:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:55:47: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:55:47: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 05:55:53: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 05:55:56: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 05:55:56: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 05:55:56: #1 total tags in treatment: 1587995 INFO @ Sat, 03 Apr 2021 05:55:56: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 05:55:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 05:55:56: #1 tags after filtering in treatment: 1587995 INFO @ Sat, 03 Apr 2021 05:55:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 05:55:56: #1 finished! INFO @ Sat, 03 Apr 2021 05:55:56: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 05:55:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 05:55:57: #2 number of paired peaks: 4449 INFO @ Sat, 03 Apr 2021 05:55:57: start model_add_line... INFO @ Sat, 03 Apr 2021 05:55:57: start X-correlation... INFO @ Sat, 03 Apr 2021 05:55:57: end of X-cor INFO @ Sat, 03 Apr 2021 05:55:57: #2 finished! INFO @ Sat, 03 Apr 2021 05:55:57: #2 predicted fragment length is 75 bps INFO @ Sat, 03 Apr 2021 05:55:57: #2 alternative fragment length(s) may be 75 bps INFO @ Sat, 03 Apr 2021 05:55:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.20_model.r WARNING @ Sat, 03 Apr 2021 05:55:57: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 05:55:57: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sat, 03 Apr 2021 05:55:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 05:55:57: #3 Call peaks... INFO @ Sat, 03 Apr 2021 05:55:57: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 05:56:00: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 05:56:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.20_peaks.xls INFO @ Sat, 03 Apr 2021 05:56:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 05:56:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246275/SRX7246275.20_summits.bed INFO @ Sat, 03 Apr 2021 05:56:02: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2260 records, 4 fields): 5 millis CompletedMACS2peakCalling