Job ID = 12264803 SRX = SRX7246260 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6789079 spots for SRR10564657/SRR10564657.sra Written 6789079 spots for SRR10564657/SRR10564657.sra Read 6928498 spots for SRR10564658/SRR10564658.sra Written 6928498 spots for SRR10564658/SRR10564658.sra Read 10377943 spots for SRR10564659/SRR10564659.sra Written 10377943 spots for SRR10564659/SRR10564659.sra Read 10377943 spots for SRR10564660/SRR10564660.sra Written 10377943 spots for SRR10564660/SRR10564660.sra Read 10493425 spots for SRR10564661/SRR10564661.sra Written 10493425 spots for SRR10564661/SRR10564661.sra Read 10493425 spots for SRR10564662/SRR10564662.sra Written 10493425 spots for SRR10564662/SRR10564662.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265223 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:24 55460313 reads; of these: 55460313 (100.00%) were unpaired; of these: 2336658 (4.21%) aligned 0 times 42146985 (75.99%) aligned exactly 1 time 10976670 (19.79%) aligned >1 times 95.79% overall alignment rate Time searching: 00:12:24 Overall time: 00:12:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 30161661 / 53123655 = 0.5678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:21:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:21:40: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:21:40: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:21:48: 1000000 INFO @ Sat, 03 Apr 2021 06:21:56: 2000000 INFO @ Sat, 03 Apr 2021 06:22:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:22:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:22:10: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:22:10: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:22:12: 4000000 INFO @ Sat, 03 Apr 2021 06:22:17: 1000000 INFO @ Sat, 03 Apr 2021 06:22:20: 5000000 INFO @ Sat, 03 Apr 2021 06:22:24: 2000000 INFO @ Sat, 03 Apr 2021 06:22:28: 6000000 INFO @ Sat, 03 Apr 2021 06:22:30: 3000000 INFO @ Sat, 03 Apr 2021 06:22:36: 7000000 INFO @ Sat, 03 Apr 2021 06:22:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:22:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:22:41: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:22:41: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:22:43: 5000000 INFO @ Sat, 03 Apr 2021 06:22:45: 8000000 INFO @ Sat, 03 Apr 2021 06:22:50: 6000000 INFO @ Sat, 03 Apr 2021 06:22:50: 1000000 INFO @ Sat, 03 Apr 2021 06:22:54: 9000000 INFO @ Sat, 03 Apr 2021 06:22:58: 7000000 INFO @ Sat, 03 Apr 2021 06:23:00: 2000000 INFO @ Sat, 03 Apr 2021 06:23:03: 10000000 INFO @ Sat, 03 Apr 2021 06:23:08: 3000000 INFO @ Sat, 03 Apr 2021 06:23:08: 8000000 INFO @ Sat, 03 Apr 2021 06:23:13: 11000000 INFO @ Sat, 03 Apr 2021 06:23:16: 9000000 INFO @ Sat, 03 Apr 2021 06:23:18: 4000000 INFO @ Sat, 03 Apr 2021 06:23:22: 10000000 INFO @ Sat, 03 Apr 2021 06:23:24: 12000000 INFO @ Sat, 03 Apr 2021 06:23:27: 5000000 INFO @ Sat, 03 Apr 2021 06:23:30: 11000000 INFO @ Sat, 03 Apr 2021 06:23:33: 13000000 INFO @ Sat, 03 Apr 2021 06:23:36: 6000000 INFO @ Sat, 03 Apr 2021 06:23:38: 12000000 INFO @ Sat, 03 Apr 2021 06:23:42: 14000000 INFO @ Sat, 03 Apr 2021 06:23:45: 7000000 INFO @ Sat, 03 Apr 2021 06:23:47: 13000000 INFO @ Sat, 03 Apr 2021 06:23:51: 15000000 INFO @ Sat, 03 Apr 2021 06:23:54: 8000000 INFO @ Sat, 03 Apr 2021 06:23:55: 14000000 INFO @ Sat, 03 Apr 2021 06:24:00: 16000000 INFO @ Sat, 03 Apr 2021 06:24:02: 15000000 INFO @ Sat, 03 Apr 2021 06:24:03: 9000000 INFO @ Sat, 03 Apr 2021 06:24:08: 17000000 INFO @ Sat, 03 Apr 2021 06:24:10: 16000000 INFO @ Sat, 03 Apr 2021 06:24:11: 10000000 INFO @ Sat, 03 Apr 2021 06:24:16: 18000000 INFO @ Sat, 03 Apr 2021 06:24:17: 17000000 INFO @ Sat, 03 Apr 2021 06:24:19: 11000000 INFO @ Sat, 03 Apr 2021 06:24:24: 18000000 INFO @ Sat, 03 Apr 2021 06:24:24: 19000000 INFO @ Sat, 03 Apr 2021 06:24:26: 12000000 INFO @ Sat, 03 Apr 2021 06:24:31: 19000000 INFO @ Sat, 03 Apr 2021 06:24:32: 20000000 INFO @ Sat, 03 Apr 2021 06:24:34: 13000000 INFO @ Sat, 03 Apr 2021 06:24:39: 20000000 INFO @ Sat, 03 Apr 2021 06:24:40: 21000000 INFO @ Sat, 03 Apr 2021 06:24:42: 14000000 INFO @ Sat, 03 Apr 2021 06:24:47: 21000000 INFO @ Sat, 03 Apr 2021 06:24:49: 22000000 INFO @ Sat, 03 Apr 2021 06:24:50: 15000000 INFO @ Sat, 03 Apr 2021 06:24:54: 22000000 INFO @ Sat, 03 Apr 2021 06:24:56: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:24:56: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:24:56: #1 total tags in treatment: 22961994 INFO @ Sat, 03 Apr 2021 06:24:56: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:24:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:24:57: #1 tags after filtering in treatment: 22961994 INFO @ Sat, 03 Apr 2021 06:24:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:24:57: #1 finished! INFO @ Sat, 03 Apr 2021 06:24:57: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:24:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:24:58: 16000000 INFO @ Sat, 03 Apr 2021 06:24:58: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 06:24:58: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:24:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:25:02: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:25:02: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:25:02: #1 total tags in treatment: 22961994 INFO @ Sat, 03 Apr 2021 06:25:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:25:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:25:02: #1 tags after filtering in treatment: 22961994 INFO @ Sat, 03 Apr 2021 06:25:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:25:02: #1 finished! INFO @ Sat, 03 Apr 2021 06:25:02: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:25:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:25:04: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 06:25:04: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:25:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:25:05: 17000000 INFO @ Sat, 03 Apr 2021 06:25:13: 18000000 INFO @ Sat, 03 Apr 2021 06:25:19: 19000000 INFO @ Sat, 03 Apr 2021 06:25:26: 20000000 INFO @ Sat, 03 Apr 2021 06:25:33: 21000000 INFO @ Sat, 03 Apr 2021 06:25:39: 22000000 INFO @ Sat, 03 Apr 2021 06:25:45: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:25:45: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:25:45: #1 total tags in treatment: 22961994 INFO @ Sat, 03 Apr 2021 06:25:45: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:25:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:25:46: #1 tags after filtering in treatment: 22961994 INFO @ Sat, 03 Apr 2021 06:25:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:25:46: #1 finished! INFO @ Sat, 03 Apr 2021 06:25:46: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:25:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:25:47: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 06:25:47: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:25:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7246260/SRX7246260.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。