Job ID = 12264800 SRX = SRX7246257 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 3415204 spots for SRR10564641/SRR10564641.sra Written 3415204 spots for SRR10564641/SRR10564641.sra Read 3524477 spots for SRR10564642/SRR10564642.sra Written 3524477 spots for SRR10564642/SRR10564642.sra Read 3614729 spots for SRR10564643/SRR10564643.sra Written 3614729 spots for SRR10564643/SRR10564643.sra Read 3812738 spots for SRR10564644/SRR10564644.sra Written 3812738 spots for SRR10564644/SRR10564644.sra Read 6477281 spots for SRR10564645/SRR10564645.sra Written 6477281 spots for SRR10564645/SRR10564645.sra Read 6477281 spots for SRR10564646/SRR10564646.sra Written 6477281 spots for SRR10564646/SRR10564646.sra Read 6468419 spots for SRR10564647/SRR10564647.sra Written 6468419 spots for SRR10564647/SRR10564647.sra Read 6468419 spots for SRR10564648/SRR10564648.sra Written 6468419 spots for SRR10564648/SRR10564648.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265139 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:35 40258548 reads; of these: 40258548 (100.00%) were unpaired; of these: 1394676 (3.46%) aligned 0 times 34395631 (85.44%) aligned exactly 1 time 4468241 (11.10%) aligned >1 times 96.54% overall alignment rate Time searching: 00:07:35 Overall time: 00:07:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 27685085 / 38863872 = 0.7124 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:11:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:11:40: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:11:40: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:11:46: 1000000 INFO @ Sat, 03 Apr 2021 06:11:54: 2000000 INFO @ Sat, 03 Apr 2021 06:12:02: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:12:10: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:12:10: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:12:10: 4000000 INFO @ Sat, 03 Apr 2021 06:12:18: 1000000 INFO @ Sat, 03 Apr 2021 06:12:18: 5000000 INFO @ Sat, 03 Apr 2021 06:12:25: 2000000 INFO @ Sat, 03 Apr 2021 06:12:25: 6000000 INFO @ Sat, 03 Apr 2021 06:12:31: 3000000 INFO @ Sat, 03 Apr 2021 06:12:31: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:12:39: 4000000 INFO @ Sat, 03 Apr 2021 06:12:39: 8000000 INFO @ Sat, 03 Apr 2021 06:12:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:12:40: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:12:40: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:12:46: 5000000 INFO @ Sat, 03 Apr 2021 06:12:46: 9000000 INFO @ Sat, 03 Apr 2021 06:12:47: 1000000 INFO @ Sat, 03 Apr 2021 06:12:53: 6000000 INFO @ Sat, 03 Apr 2021 06:12:54: 10000000 INFO @ Sat, 03 Apr 2021 06:12:55: 2000000 INFO @ Sat, 03 Apr 2021 06:13:01: 7000000 INFO @ Sat, 03 Apr 2021 06:13:01: 11000000 INFO @ Sat, 03 Apr 2021 06:13:02: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:13:02: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:13:02: #1 total tags in treatment: 11178787 INFO @ Sat, 03 Apr 2021 06:13:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:13:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:13:02: 3000000 INFO @ Sat, 03 Apr 2021 06:13:02: #1 tags after filtering in treatment: 11178787 INFO @ Sat, 03 Apr 2021 06:13:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:13:02: #1 finished! INFO @ Sat, 03 Apr 2021 06:13:02: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:13:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:13:03: #2 number of paired peaks: 1649 INFO @ Sat, 03 Apr 2021 06:13:03: start model_add_line... INFO @ Sat, 03 Apr 2021 06:13:03: start X-correlation... INFO @ Sat, 03 Apr 2021 06:13:03: end of X-cor INFO @ Sat, 03 Apr 2021 06:13:03: #2 finished! INFO @ Sat, 03 Apr 2021 06:13:03: #2 predicted fragment length is 97 bps INFO @ Sat, 03 Apr 2021 06:13:03: #2 alternative fragment length(s) may be 97 bps INFO @ Sat, 03 Apr 2021 06:13:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.05_model.r WARNING @ Sat, 03 Apr 2021 06:13:03: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:13:03: #2 You may need to consider one of the other alternative d(s): 97 WARNING @ Sat, 03 Apr 2021 06:13:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:13:03: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:13:03: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:13:08: 8000000 INFO @ Sat, 03 Apr 2021 06:13:09: 4000000 INFO @ Sat, 03 Apr 2021 06:13:14: 9000000 INFO @ Sat, 03 Apr 2021 06:13:16: 5000000 INFO @ Sat, 03 Apr 2021 06:13:21: 10000000 INFO @ Sat, 03 Apr 2021 06:13:22: 6000000 INFO @ Sat, 03 Apr 2021 06:13:27: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:13:28: 11000000 INFO @ Sat, 03 Apr 2021 06:13:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:13:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:13:29: #1 total tags in treatment: 11178787 INFO @ Sat, 03 Apr 2021 06:13:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:13:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:13:29: #1 tags after filtering in treatment: 11178787 INFO @ Sat, 03 Apr 2021 06:13:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:13:29: #1 finished! INFO @ Sat, 03 Apr 2021 06:13:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:13:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:13:29: 7000000 INFO @ Sat, 03 Apr 2021 06:13:30: #2 number of paired peaks: 1649 INFO @ Sat, 03 Apr 2021 06:13:30: start model_add_line... INFO @ Sat, 03 Apr 2021 06:13:30: start X-correlation... INFO @ Sat, 03 Apr 2021 06:13:30: end of X-cor INFO @ Sat, 03 Apr 2021 06:13:30: #2 finished! INFO @ Sat, 03 Apr 2021 06:13:30: #2 predicted fragment length is 97 bps INFO @ Sat, 03 Apr 2021 06:13:30: #2 alternative fragment length(s) may be 97 bps INFO @ Sat, 03 Apr 2021 06:13:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.10_model.r WARNING @ Sat, 03 Apr 2021 06:13:30: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:13:30: #2 You may need to consider one of the other alternative d(s): 97 WARNING @ Sat, 03 Apr 2021 06:13:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:13:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:13:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:13:35: 8000000 INFO @ Sat, 03 Apr 2021 06:13:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:13:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:13:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.05_summits.bed INFO @ Sat, 03 Apr 2021 06:13:38: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10985 records, 4 fields): 14 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:13:42: 9000000 INFO @ Sat, 03 Apr 2021 06:13:49: 10000000 INFO @ Sat, 03 Apr 2021 06:13:53: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:13:56: 11000000 INFO @ Sat, 03 Apr 2021 06:13:57: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:13:57: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:13:57: #1 total tags in treatment: 11178787 INFO @ Sat, 03 Apr 2021 06:13:57: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:13:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:13:57: #1 tags after filtering in treatment: 11178787 INFO @ Sat, 03 Apr 2021 06:13:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:13:57: #1 finished! INFO @ Sat, 03 Apr 2021 06:13:57: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:13:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:13:58: #2 number of paired peaks: 1649 INFO @ Sat, 03 Apr 2021 06:13:58: start model_add_line... INFO @ Sat, 03 Apr 2021 06:13:58: start X-correlation... INFO @ Sat, 03 Apr 2021 06:13:58: end of X-cor INFO @ Sat, 03 Apr 2021 06:13:58: #2 finished! INFO @ Sat, 03 Apr 2021 06:13:58: #2 predicted fragment length is 97 bps INFO @ Sat, 03 Apr 2021 06:13:58: #2 alternative fragment length(s) may be 97 bps INFO @ Sat, 03 Apr 2021 06:13:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.20_model.r WARNING @ Sat, 03 Apr 2021 06:13:58: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:13:58: #2 You may need to consider one of the other alternative d(s): 97 WARNING @ Sat, 03 Apr 2021 06:13:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:13:58: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:13:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:14:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:14:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:14:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.10_summits.bed INFO @ Sat, 03 Apr 2021 06:14:06: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8014 records, 4 fields): 11 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:14:22: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:14:34: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:14:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:14:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7246257/SRX7246257.20_summits.bed INFO @ Sat, 03 Apr 2021 06:14:34: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (4828 records, 4 fields): 8 millis CompletedMACS2peakCalling