Job ID = 5720230 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T13:36:40 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:42:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:44:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:48:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:53:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:57:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 38,292,397 reads read : 76,584,794 reads written : 76,584,794 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:04:13 38292397 reads; of these: 38292397 (100.00%) were paired; of these: 16181559 (42.26%) aligned concordantly 0 times 18505671 (48.33%) aligned concordantly exactly 1 time 3605167 (9.41%) aligned concordantly >1 times ---- 16181559 pairs aligned concordantly 0 times; of these: 6466398 (39.96%) aligned discordantly 1 time ---- 9715161 pairs aligned 0 times concordantly or discordantly; of these: 19430322 mates make up the pairs; of these: 16693083 (85.91%) aligned 0 times 924739 (4.76%) aligned exactly 1 time 1812500 (9.33%) aligned >1 times 78.20% overall alignment rate Time searching: 01:04:13 Overall time: 01:04:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4425525 / 28352583 = 0.1561 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:28:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:28:41: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:28:41: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:28:48: 1000000 INFO @ Thu, 16 Apr 2020 00:28:55: 2000000 INFO @ Thu, 16 Apr 2020 00:29:02: 3000000 INFO @ Thu, 16 Apr 2020 00:29:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:29:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:29:11: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:29:11: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:29:15: 5000000 INFO @ Thu, 16 Apr 2020 00:29:19: 1000000 INFO @ Thu, 16 Apr 2020 00:29:23: 6000000 INFO @ Thu, 16 Apr 2020 00:29:27: 2000000 INFO @ Thu, 16 Apr 2020 00:29:29: 7000000 INFO @ Thu, 16 Apr 2020 00:29:35: 3000000 INFO @ Thu, 16 Apr 2020 00:29:36: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:29:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:29:41: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:29:41: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:29:43: 4000000 INFO @ Thu, 16 Apr 2020 00:29:43: 9000000 INFO @ Thu, 16 Apr 2020 00:29:49: 1000000 INFO @ Thu, 16 Apr 2020 00:29:50: 10000000 INFO @ Thu, 16 Apr 2020 00:29:51: 5000000 INFO @ Thu, 16 Apr 2020 00:29:57: 11000000 INFO @ Thu, 16 Apr 2020 00:29:58: 2000000 INFO @ Thu, 16 Apr 2020 00:29:59: 6000000 INFO @ Thu, 16 Apr 2020 00:30:05: 12000000 INFO @ Thu, 16 Apr 2020 00:30:06: 3000000 INFO @ Thu, 16 Apr 2020 00:30:08: 7000000 INFO @ Thu, 16 Apr 2020 00:30:12: 13000000 INFO @ Thu, 16 Apr 2020 00:30:14: 4000000 INFO @ Thu, 16 Apr 2020 00:30:16: 8000000 INFO @ Thu, 16 Apr 2020 00:30:19: 14000000 INFO @ Thu, 16 Apr 2020 00:30:22: 5000000 INFO @ Thu, 16 Apr 2020 00:30:24: 9000000 INFO @ Thu, 16 Apr 2020 00:30:26: 15000000 INFO @ Thu, 16 Apr 2020 00:30:30: 6000000 INFO @ Thu, 16 Apr 2020 00:30:31: 10000000 INFO @ Thu, 16 Apr 2020 00:30:33: 16000000 INFO @ Thu, 16 Apr 2020 00:30:38: 7000000 INFO @ Thu, 16 Apr 2020 00:30:40: 11000000 INFO @ Thu, 16 Apr 2020 00:30:40: 17000000 INFO @ Thu, 16 Apr 2020 00:30:46: 8000000 INFO @ Thu, 16 Apr 2020 00:30:47: 18000000 INFO @ Thu, 16 Apr 2020 00:30:48: 12000000 INFO @ Thu, 16 Apr 2020 00:30:54: 9000000 INFO @ Thu, 16 Apr 2020 00:30:55: 19000000 INFO @ Thu, 16 Apr 2020 00:30:56: 13000000 INFO @ Thu, 16 Apr 2020 00:31:02: 20000000 INFO @ Thu, 16 Apr 2020 00:31:02: 10000000 INFO @ Thu, 16 Apr 2020 00:31:04: 14000000 INFO @ Thu, 16 Apr 2020 00:31:09: 21000000 INFO @ Thu, 16 Apr 2020 00:31:10: 11000000 INFO @ Thu, 16 Apr 2020 00:31:12: 15000000 INFO @ Thu, 16 Apr 2020 00:31:16: 22000000 INFO @ Thu, 16 Apr 2020 00:31:19: 12000000 INFO @ Thu, 16 Apr 2020 00:31:20: 16000000 INFO @ Thu, 16 Apr 2020 00:31:23: 23000000 INFO @ Thu, 16 Apr 2020 00:31:27: 13000000 INFO @ Thu, 16 Apr 2020 00:31:28: 17000000 INFO @ Thu, 16 Apr 2020 00:31:30: 24000000 INFO @ Thu, 16 Apr 2020 00:31:35: 14000000 INFO @ Thu, 16 Apr 2020 00:31:37: 18000000 INFO @ Thu, 16 Apr 2020 00:31:37: 25000000 INFO @ Thu, 16 Apr 2020 00:31:43: 15000000 INFO @ Thu, 16 Apr 2020 00:31:45: 26000000 INFO @ Thu, 16 Apr 2020 00:31:45: 19000000 INFO @ Thu, 16 Apr 2020 00:31:51: 16000000 INFO @ Thu, 16 Apr 2020 00:31:52: 27000000 INFO @ Thu, 16 Apr 2020 00:31:53: 20000000 INFO @ Thu, 16 Apr 2020 00:31:59: 28000000 INFO @ Thu, 16 Apr 2020 00:32:00: 17000000 INFO @ Thu, 16 Apr 2020 00:32:01: 21000000 INFO @ Thu, 16 Apr 2020 00:32:06: 29000000 INFO @ Thu, 16 Apr 2020 00:32:08: 18000000 INFO @ Thu, 16 Apr 2020 00:32:10: 22000000 INFO @ Thu, 16 Apr 2020 00:32:13: 30000000 INFO @ Thu, 16 Apr 2020 00:32:16: 19000000 INFO @ Thu, 16 Apr 2020 00:32:19: 23000000 INFO @ Thu, 16 Apr 2020 00:32:20: 31000000 INFO @ Thu, 16 Apr 2020 00:32:24: 20000000 INFO @ Thu, 16 Apr 2020 00:32:28: 24000000 INFO @ Thu, 16 Apr 2020 00:32:30: 32000000 INFO @ Thu, 16 Apr 2020 00:32:32: 21000000 INFO @ Thu, 16 Apr 2020 00:32:38: 25000000 INFO @ Thu, 16 Apr 2020 00:32:40: 33000000 INFO @ Thu, 16 Apr 2020 00:32:41: 22000000 INFO @ Thu, 16 Apr 2020 00:32:49: 26000000 INFO @ Thu, 16 Apr 2020 00:32:51: 34000000 INFO @ Thu, 16 Apr 2020 00:32:51: 23000000 INFO @ Thu, 16 Apr 2020 00:32:59: 27000000 INFO @ Thu, 16 Apr 2020 00:33:00: 35000000 INFO @ Thu, 16 Apr 2020 00:33:01: 24000000 INFO @ Thu, 16 Apr 2020 00:33:09: 28000000 INFO @ Thu, 16 Apr 2020 00:33:11: 25000000 INFO @ Thu, 16 Apr 2020 00:33:11: 36000000 INFO @ Thu, 16 Apr 2020 00:33:20: 29000000 INFO @ Thu, 16 Apr 2020 00:33:21: 26000000 INFO @ Thu, 16 Apr 2020 00:33:22: 37000000 INFO @ Thu, 16 Apr 2020 00:33:30: 30000000 INFO @ Thu, 16 Apr 2020 00:33:33: 27000000 INFO @ Thu, 16 Apr 2020 00:33:34: 38000000 INFO @ Thu, 16 Apr 2020 00:33:42: 31000000 INFO @ Thu, 16 Apr 2020 00:33:43: 28000000 INFO @ Thu, 16 Apr 2020 00:33:45: 39000000 INFO @ Thu, 16 Apr 2020 00:33:51: 32000000 INFO @ Thu, 16 Apr 2020 00:33:54: 29000000 INFO @ Thu, 16 Apr 2020 00:33:56: 40000000 INFO @ Thu, 16 Apr 2020 00:34:03: 33000000 INFO @ Thu, 16 Apr 2020 00:34:05: 30000000 INFO @ Thu, 16 Apr 2020 00:34:06: 41000000 INFO @ Thu, 16 Apr 2020 00:34:15: 31000000 INFO @ Thu, 16 Apr 2020 00:34:16: 34000000 INFO @ Thu, 16 Apr 2020 00:34:16: 42000000 INFO @ Thu, 16 Apr 2020 00:34:25: 32000000 INFO @ Thu, 16 Apr 2020 00:34:27: 35000000 INFO @ Thu, 16 Apr 2020 00:34:27: 43000000 INFO @ Thu, 16 Apr 2020 00:34:37: 36000000 INFO @ Thu, 16 Apr 2020 00:34:37: 33000000 INFO @ Thu, 16 Apr 2020 00:34:38: 44000000 INFO @ Thu, 16 Apr 2020 00:34:46: 37000000 INFO @ Thu, 16 Apr 2020 00:34:48: 34000000 INFO @ Thu, 16 Apr 2020 00:34:50: 45000000 INFO @ Thu, 16 Apr 2020 00:34:58: 38000000 INFO @ Thu, 16 Apr 2020 00:34:59: 35000000 INFO @ Thu, 16 Apr 2020 00:35:00: 46000000 INFO @ Thu, 16 Apr 2020 00:35:09: 39000000 INFO @ Thu, 16 Apr 2020 00:35:10: 47000000 INFO @ Thu, 16 Apr 2020 00:35:11: 36000000 INFO @ Thu, 16 Apr 2020 00:35:19: 40000000 INFO @ Thu, 16 Apr 2020 00:35:20: 48000000 INFO @ Thu, 16 Apr 2020 00:35:22: 37000000 INFO @ Thu, 16 Apr 2020 00:35:30: 49000000 INFO @ Thu, 16 Apr 2020 00:35:30: 41000000 INFO @ Thu, 16 Apr 2020 00:35:34: 38000000 INFO @ Thu, 16 Apr 2020 00:35:42: 50000000 INFO @ Thu, 16 Apr 2020 00:35:42: 42000000 INFO @ Thu, 16 Apr 2020 00:35:44: 39000000 INFO @ Thu, 16 Apr 2020 00:35:53: 43000000 INFO @ Thu, 16 Apr 2020 00:35:54: 51000000 INFO @ Thu, 16 Apr 2020 00:35:54: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:35:54: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:35:54: #1 total tags in treatment: 18579002 INFO @ Thu, 16 Apr 2020 00:35:54: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:35:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:35:54: 40000000 INFO @ Thu, 16 Apr 2020 00:35:55: #1 tags after filtering in treatment: 15685388 INFO @ Thu, 16 Apr 2020 00:35:55: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:35:55: #1 finished! INFO @ Thu, 16 Apr 2020 00:35:55: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:35:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:35:56: #2 number of paired peaks: 313 WARNING @ Thu, 16 Apr 2020 00:35:56: Fewer paired peaks (313) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 313 pairs to build model! INFO @ Thu, 16 Apr 2020 00:35:56: start model_add_line... INFO @ Thu, 16 Apr 2020 00:35:56: start X-correlation... INFO @ Thu, 16 Apr 2020 00:35:56: end of X-cor INFO @ Thu, 16 Apr 2020 00:35:56: #2 finished! INFO @ Thu, 16 Apr 2020 00:35:56: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:35:56: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:35:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.05_model.r WARNING @ Thu, 16 Apr 2020 00:35:56: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:35:56: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:35:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:35:56: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:35:56: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:36:04: 44000000 INFO @ Thu, 16 Apr 2020 00:36:05: 41000000 INFO @ Thu, 16 Apr 2020 00:36:13: 42000000 INFO @ Thu, 16 Apr 2020 00:36:14: 45000000 INFO @ Thu, 16 Apr 2020 00:36:23: 43000000 INFO @ Thu, 16 Apr 2020 00:36:24: 46000000 INFO @ Thu, 16 Apr 2020 00:36:33: 44000000 INFO @ Thu, 16 Apr 2020 00:36:35: 47000000 INFO @ Thu, 16 Apr 2020 00:36:39: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:36:43: 45000000 INFO @ Thu, 16 Apr 2020 00:36:46: 48000000 INFO @ Thu, 16 Apr 2020 00:36:52: 46000000 INFO @ Thu, 16 Apr 2020 00:36:56: 49000000 INFO @ Thu, 16 Apr 2020 00:37:01: 47000000 INFO @ Thu, 16 Apr 2020 00:37:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:37:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:37:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.05_summits.bed INFO @ Thu, 16 Apr 2020 00:37:04: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (24956 records, 4 fields): 23 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:37:07: 50000000 INFO @ Thu, 16 Apr 2020 00:37:10: 48000000 INFO @ Thu, 16 Apr 2020 00:37:17: 51000000 INFO @ Thu, 16 Apr 2020 00:37:18: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:37:18: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:37:18: #1 total tags in treatment: 18579002 INFO @ Thu, 16 Apr 2020 00:37:18: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:37:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:37:18: #1 tags after filtering in treatment: 15685388 INFO @ Thu, 16 Apr 2020 00:37:18: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:37:18: #1 finished! INFO @ Thu, 16 Apr 2020 00:37:18: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:37:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:37:19: #2 number of paired peaks: 313 WARNING @ Thu, 16 Apr 2020 00:37:19: Fewer paired peaks (313) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 313 pairs to build model! INFO @ Thu, 16 Apr 2020 00:37:19: start model_add_line... INFO @ Thu, 16 Apr 2020 00:37:19: start X-correlation... INFO @ Thu, 16 Apr 2020 00:37:19: end of X-cor INFO @ Thu, 16 Apr 2020 00:37:19: #2 finished! INFO @ Thu, 16 Apr 2020 00:37:19: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:37:19: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:37:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.10_model.r WARNING @ Thu, 16 Apr 2020 00:37:19: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:37:19: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:37:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:37:19: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:37:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:37:20: 49000000 INFO @ Thu, 16 Apr 2020 00:37:29: 50000000 INFO @ Thu, 16 Apr 2020 00:37:39: 51000000 INFO @ Thu, 16 Apr 2020 00:37:39: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:37:39: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:37:39: #1 total tags in treatment: 18579002 INFO @ Thu, 16 Apr 2020 00:37:39: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:37:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:37:40: #1 tags after filtering in treatment: 15685388 INFO @ Thu, 16 Apr 2020 00:37:40: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:37:40: #1 finished! INFO @ Thu, 16 Apr 2020 00:37:40: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:37:41: #2 number of paired peaks: 313 WARNING @ Thu, 16 Apr 2020 00:37:41: Fewer paired peaks (313) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 313 pairs to build model! INFO @ Thu, 16 Apr 2020 00:37:41: start model_add_line... INFO @ Thu, 16 Apr 2020 00:37:41: start X-correlation... INFO @ Thu, 16 Apr 2020 00:37:41: end of X-cor INFO @ Thu, 16 Apr 2020 00:37:41: #2 finished! INFO @ Thu, 16 Apr 2020 00:37:41: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:37:41: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:37:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.20_model.r WARNING @ Thu, 16 Apr 2020 00:37:41: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:37:41: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:37:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:37:41: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:37:41: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 00:38:01: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:38:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:38:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:38:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.10_summits.bed INFO @ Thu, 16 Apr 2020 00:38:19: Done! INFO @ Thu, 16 Apr 2020 00:38:20: #3 Call peaks for each chromosome... pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (11243 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:38:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:38:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:38:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619602/SRX6619602.20_summits.bed INFO @ Thu, 16 Apr 2020 00:38:40: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (1322 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。