Job ID = 5720220 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T13:01:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.238' from '172.19.7.122' 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.238) from '172.19.7.122' 2020-04-15T13:07:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:10:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:16:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:17:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:17:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:19:41 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:21:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 29,210,179 reads read : 58,420,358 reads written : 58,420,358 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:45:01 29210179 reads; of these: 29210179 (100.00%) were paired; of these: 9832833 (33.66%) aligned concordantly 0 times 14063147 (48.14%) aligned concordantly exactly 1 time 5314199 (18.19%) aligned concordantly >1 times ---- 9832833 pairs aligned concordantly 0 times; of these: 4784333 (48.66%) aligned discordantly 1 time ---- 5048500 pairs aligned 0 times concordantly or discordantly; of these: 10097000 mates make up the pairs; of these: 7568606 (74.96%) aligned 0 times 790784 (7.83%) aligned exactly 1 time 1737610 (17.21%) aligned >1 times 87.04% overall alignment rate Time searching: 01:45:01 Overall time: 01:45:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3313901 / 23847869 = 0.1390 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:44:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:44:13: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:44:13: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:44:20: 1000000 INFO @ Thu, 16 Apr 2020 00:44:28: 2000000 INFO @ Thu, 16 Apr 2020 00:44:35: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:44:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:44:43: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:44:43: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:44:43: 4000000 INFO @ Thu, 16 Apr 2020 00:44:51: 1000000 INFO @ Thu, 16 Apr 2020 00:44:51: 5000000 INFO @ Thu, 16 Apr 2020 00:44:58: 2000000 INFO @ Thu, 16 Apr 2020 00:44:59: 6000000 INFO @ Thu, 16 Apr 2020 00:45:06: 3000000 INFO @ Thu, 16 Apr 2020 00:45:07: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:45:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:45:13: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:45:13: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:45:14: 4000000 INFO @ Thu, 16 Apr 2020 00:45:15: 8000000 INFO @ Thu, 16 Apr 2020 00:45:20: 1000000 INFO @ Thu, 16 Apr 2020 00:45:22: 5000000 INFO @ Thu, 16 Apr 2020 00:45:23: 9000000 INFO @ Thu, 16 Apr 2020 00:45:27: 2000000 INFO @ Thu, 16 Apr 2020 00:45:30: 6000000 INFO @ Thu, 16 Apr 2020 00:45:32: 10000000 INFO @ Thu, 16 Apr 2020 00:45:35: 3000000 INFO @ Thu, 16 Apr 2020 00:45:39: 7000000 INFO @ Thu, 16 Apr 2020 00:45:40: 11000000 INFO @ Thu, 16 Apr 2020 00:45:41: 4000000 INFO @ Thu, 16 Apr 2020 00:45:47: 8000000 INFO @ Thu, 16 Apr 2020 00:45:48: 5000000 INFO @ Thu, 16 Apr 2020 00:45:49: 12000000 INFO @ Thu, 16 Apr 2020 00:45:55: 6000000 INFO @ Thu, 16 Apr 2020 00:45:55: 9000000 INFO @ Thu, 16 Apr 2020 00:45:57: 13000000 INFO @ Thu, 16 Apr 2020 00:46:02: 7000000 INFO @ Thu, 16 Apr 2020 00:46:04: 10000000 INFO @ Thu, 16 Apr 2020 00:46:06: 14000000 INFO @ Thu, 16 Apr 2020 00:46:09: 8000000 INFO @ Thu, 16 Apr 2020 00:46:12: 11000000 INFO @ Thu, 16 Apr 2020 00:46:15: 15000000 INFO @ Thu, 16 Apr 2020 00:46:15: 9000000 INFO @ Thu, 16 Apr 2020 00:46:21: 12000000 INFO @ Thu, 16 Apr 2020 00:46:22: 10000000 INFO @ Thu, 16 Apr 2020 00:46:24: 16000000 INFO @ Thu, 16 Apr 2020 00:46:29: 13000000 INFO @ Thu, 16 Apr 2020 00:46:29: 11000000 INFO @ Thu, 16 Apr 2020 00:46:32: 17000000 INFO @ Thu, 16 Apr 2020 00:46:36: 12000000 INFO @ Thu, 16 Apr 2020 00:46:37: 14000000 INFO @ Thu, 16 Apr 2020 00:46:42: 18000000 INFO @ Thu, 16 Apr 2020 00:46:43: 13000000 INFO @ Thu, 16 Apr 2020 00:46:45: 15000000 INFO @ Thu, 16 Apr 2020 00:46:50: 14000000 INFO @ Thu, 16 Apr 2020 00:46:50: 19000000 INFO @ Thu, 16 Apr 2020 00:46:53: 16000000 INFO @ Thu, 16 Apr 2020 00:46:57: 15000000 INFO @ Thu, 16 Apr 2020 00:46:59: 20000000 INFO @ Thu, 16 Apr 2020 00:47:02: 17000000 INFO @ Thu, 16 Apr 2020 00:47:03: 16000000 INFO @ Thu, 16 Apr 2020 00:47:08: 21000000 INFO @ Thu, 16 Apr 2020 00:47:10: 17000000 INFO @ Thu, 16 Apr 2020 00:47:11: 18000000 INFO @ Thu, 16 Apr 2020 00:47:16: 22000000 INFO @ Thu, 16 Apr 2020 00:47:17: 18000000 INFO @ Thu, 16 Apr 2020 00:47:19: 19000000 INFO @ Thu, 16 Apr 2020 00:47:24: 19000000 INFO @ Thu, 16 Apr 2020 00:47:25: 23000000 INFO @ Thu, 16 Apr 2020 00:47:27: 20000000 INFO @ Thu, 16 Apr 2020 00:47:31: 20000000 INFO @ Thu, 16 Apr 2020 00:47:32: 24000000 INFO @ Thu, 16 Apr 2020 00:47:35: 21000000 INFO @ Thu, 16 Apr 2020 00:47:38: 21000000 INFO @ Thu, 16 Apr 2020 00:47:41: 25000000 INFO @ Thu, 16 Apr 2020 00:47:43: 22000000 INFO @ Thu, 16 Apr 2020 00:47:45: 22000000 INFO @ Thu, 16 Apr 2020 00:47:49: 26000000 INFO @ Thu, 16 Apr 2020 00:47:52: 23000000 INFO @ Thu, 16 Apr 2020 00:47:52: 23000000 INFO @ Thu, 16 Apr 2020 00:47:57: 27000000 INFO @ Thu, 16 Apr 2020 00:47:58: 24000000 INFO @ Thu, 16 Apr 2020 00:48:00: 24000000 INFO @ Thu, 16 Apr 2020 00:48:05: 25000000 INFO @ Thu, 16 Apr 2020 00:48:06: 28000000 INFO @ Thu, 16 Apr 2020 00:48:09: 25000000 INFO @ Thu, 16 Apr 2020 00:48:12: 26000000 INFO @ Thu, 16 Apr 2020 00:48:14: 29000000 INFO @ Thu, 16 Apr 2020 00:48:17: 26000000 INFO @ Thu, 16 Apr 2020 00:48:19: 27000000 INFO @ Thu, 16 Apr 2020 00:48:22: 30000000 INFO @ Thu, 16 Apr 2020 00:48:25: 27000000 INFO @ Thu, 16 Apr 2020 00:48:26: 28000000 INFO @ Thu, 16 Apr 2020 00:48:31: 31000000 INFO @ Thu, 16 Apr 2020 00:48:33: 29000000 INFO @ Thu, 16 Apr 2020 00:48:34: 28000000 INFO @ Thu, 16 Apr 2020 00:48:40: 32000000 INFO @ Thu, 16 Apr 2020 00:48:40: 30000000 INFO @ Thu, 16 Apr 2020 00:48:42: 29000000 INFO @ Thu, 16 Apr 2020 00:48:47: 31000000 INFO @ Thu, 16 Apr 2020 00:48:48: 33000000 INFO @ Thu, 16 Apr 2020 00:48:50: 30000000 INFO @ Thu, 16 Apr 2020 00:48:54: 32000000 INFO @ Thu, 16 Apr 2020 00:48:56: 34000000 INFO @ Thu, 16 Apr 2020 00:48:59: 31000000 INFO @ Thu, 16 Apr 2020 00:49:01: 33000000 INFO @ Thu, 16 Apr 2020 00:49:04: 35000000 INFO @ Thu, 16 Apr 2020 00:49:07: 32000000 INFO @ Thu, 16 Apr 2020 00:49:09: 34000000 INFO @ Thu, 16 Apr 2020 00:49:12: 36000000 INFO @ Thu, 16 Apr 2020 00:49:15: 33000000 INFO @ Thu, 16 Apr 2020 00:49:16: 35000000 INFO @ Thu, 16 Apr 2020 00:49:20: 37000000 INFO @ Thu, 16 Apr 2020 00:49:23: 36000000 INFO @ Thu, 16 Apr 2020 00:49:23: 34000000 INFO @ Thu, 16 Apr 2020 00:49:29: 38000000 INFO @ Thu, 16 Apr 2020 00:49:30: 37000000 INFO @ Thu, 16 Apr 2020 00:49:31: 35000000 INFO @ Thu, 16 Apr 2020 00:49:36: 38000000 INFO @ Thu, 16 Apr 2020 00:49:37: 39000000 INFO @ Thu, 16 Apr 2020 00:49:39: 36000000 INFO @ Thu, 16 Apr 2020 00:49:43: 39000000 INFO @ Thu, 16 Apr 2020 00:49:45: 40000000 INFO @ Thu, 16 Apr 2020 00:49:47: 37000000 INFO @ Thu, 16 Apr 2020 00:49:50: 40000000 INFO @ Thu, 16 Apr 2020 00:49:53: 41000000 INFO @ Thu, 16 Apr 2020 00:49:56: 38000000 INFO @ Thu, 16 Apr 2020 00:49:57: 41000000 INFO @ Thu, 16 Apr 2020 00:50:02: 42000000 INFO @ Thu, 16 Apr 2020 00:50:04: 42000000 INFO @ Thu, 16 Apr 2020 00:50:04: 39000000 INFO @ Thu, 16 Apr 2020 00:50:10: 43000000 INFO @ Thu, 16 Apr 2020 00:50:11: 43000000 INFO @ Thu, 16 Apr 2020 00:50:13: 40000000 INFO @ Thu, 16 Apr 2020 00:50:18: 44000000 INFO @ Thu, 16 Apr 2020 00:50:19: 44000000 INFO @ Thu, 16 Apr 2020 00:50:20: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:50:20: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:50:20: #1 total tags in treatment: 16502875 INFO @ Thu, 16 Apr 2020 00:50:20: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:50:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:50:20: #1 tags after filtering in treatment: 12865158 INFO @ Thu, 16 Apr 2020 00:50:20: #1 Redundant rate of treatment: 0.22 INFO @ Thu, 16 Apr 2020 00:50:20: #1 finished! INFO @ Thu, 16 Apr 2020 00:50:20: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:50:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:50:21: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:50:21: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:50:21: #1 total tags in treatment: 16502875 INFO @ Thu, 16 Apr 2020 00:50:21: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:50:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:50:21: #2 number of paired peaks: 535 WARNING @ Thu, 16 Apr 2020 00:50:21: Fewer paired peaks (535) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 535 pairs to build model! INFO @ Thu, 16 Apr 2020 00:50:21: start model_add_line... INFO @ Thu, 16 Apr 2020 00:50:21: start X-correlation... INFO @ Thu, 16 Apr 2020 00:50:21: end of X-cor INFO @ Thu, 16 Apr 2020 00:50:21: #2 finished! INFO @ Thu, 16 Apr 2020 00:50:21: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:50:21: #2 alternative fragment length(s) may be 4,201 bps INFO @ Thu, 16 Apr 2020 00:50:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.20_model.r WARNING @ Thu, 16 Apr 2020 00:50:21: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:50:21: #2 You may need to consider one of the other alternative d(s): 4,201 WARNING @ Thu, 16 Apr 2020 00:50:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:50:21: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:50:21: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:50:21: #1 tags after filtering in treatment: 12865158 INFO @ Thu, 16 Apr 2020 00:50:21: #1 Redundant rate of treatment: 0.22 INFO @ Thu, 16 Apr 2020 00:50:21: #1 finished! INFO @ Thu, 16 Apr 2020 00:50:21: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:50:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:50:22: 41000000 INFO @ Thu, 16 Apr 2020 00:50:22: #2 number of paired peaks: 535 WARNING @ Thu, 16 Apr 2020 00:50:22: Fewer paired peaks (535) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 535 pairs to build model! INFO @ Thu, 16 Apr 2020 00:50:22: start model_add_line... INFO @ Thu, 16 Apr 2020 00:50:22: start X-correlation... INFO @ Thu, 16 Apr 2020 00:50:22: end of X-cor INFO @ Thu, 16 Apr 2020 00:50:22: #2 finished! INFO @ Thu, 16 Apr 2020 00:50:22: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:50:22: #2 alternative fragment length(s) may be 4,201 bps INFO @ Thu, 16 Apr 2020 00:50:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.05_model.r WARNING @ Thu, 16 Apr 2020 00:50:22: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:50:22: #2 You may need to consider one of the other alternative d(s): 4,201 WARNING @ Thu, 16 Apr 2020 00:50:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:50:22: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:50:22: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:50:29: 42000000 INFO @ Thu, 16 Apr 2020 00:50:37: 43000000 INFO @ Thu, 16 Apr 2020 00:50:45: 44000000 INFO @ Thu, 16 Apr 2020 00:50:47: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:50:47: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:50:47: #1 total tags in treatment: 16502875 INFO @ Thu, 16 Apr 2020 00:50:47: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:50:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:50:47: #1 tags after filtering in treatment: 12865158 INFO @ Thu, 16 Apr 2020 00:50:47: #1 Redundant rate of treatment: 0.22 INFO @ Thu, 16 Apr 2020 00:50:47: #1 finished! INFO @ Thu, 16 Apr 2020 00:50:47: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:50:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:50:48: #2 number of paired peaks: 535 WARNING @ Thu, 16 Apr 2020 00:50:48: Fewer paired peaks (535) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 535 pairs to build model! INFO @ Thu, 16 Apr 2020 00:50:48: start model_add_line... INFO @ Thu, 16 Apr 2020 00:50:48: start X-correlation... INFO @ Thu, 16 Apr 2020 00:50:48: end of X-cor INFO @ Thu, 16 Apr 2020 00:50:48: #2 finished! INFO @ Thu, 16 Apr 2020 00:50:48: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:50:48: #2 alternative fragment length(s) may be 4,201 bps INFO @ Thu, 16 Apr 2020 00:50:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.10_model.r WARNING @ Thu, 16 Apr 2020 00:50:48: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:50:48: #2 You may need to consider one of the other alternative d(s): 4,201 WARNING @ Thu, 16 Apr 2020 00:50:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:50:48: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:50:48: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:50:50: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:50:52: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:51:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:51:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:51:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.20_summits.bed INFO @ Thu, 16 Apr 2020 00:51:03: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (320 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:51:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:51:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:51:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.05_summits.bed INFO @ Thu, 16 Apr 2020 00:51:06: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (850 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:51:18: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:51:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:51:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:51:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619592/SRX6619592.10_summits.bed INFO @ Thu, 16 Apr 2020 00:51:31: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (500 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。