Job ID = 5720217 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:35:17 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:36:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:37:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:56:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:56:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:56:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:11:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:25:12 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:25:12 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.235' from '172.19.7.207' 2020-04-15T13:25:12 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.235) from '172.19.7.207' 2020-04-15T13:26:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 39,256,378 reads read : 78,512,756 reads written : 78,512,756 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:03:37 39256378 reads; of these: 39256378 (100.00%) were paired; of these: 15923986 (40.56%) aligned concordantly 0 times 19442848 (49.53%) aligned concordantly exactly 1 time 3889544 (9.91%) aligned concordantly >1 times ---- 15923986 pairs aligned concordantly 0 times; of these: 6461436 (40.58%) aligned discordantly 1 time ---- 9462550 pairs aligned 0 times concordantly or discordantly; of these: 18925100 mates make up the pairs; of these: 15941259 (84.23%) aligned 0 times 1006184 (5.32%) aligned exactly 1 time 1977657 (10.45%) aligned >1 times 79.70% overall alignment rate Time searching: 01:03:37 Overall time: 01:03:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4828858 / 29588196 = 0.1632 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:57:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:57:45: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:57:45: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:57:54: 1000000 INFO @ Wed, 15 Apr 2020 23:58:02: 2000000 INFO @ Wed, 15 Apr 2020 23:58:11: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:58:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:58:15: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:58:15: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:58:20: 4000000 INFO @ Wed, 15 Apr 2020 23:58:23: 1000000 INFO @ Wed, 15 Apr 2020 23:58:29: 5000000 INFO @ Wed, 15 Apr 2020 23:58:31: 2000000 INFO @ Wed, 15 Apr 2020 23:58:38: 6000000 INFO @ Wed, 15 Apr 2020 23:58:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:58:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:58:45: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:58:45: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:58:46: 4000000 INFO @ Wed, 15 Apr 2020 23:58:47: 7000000 INFO @ Wed, 15 Apr 2020 23:58:53: 1000000 INFO @ Wed, 15 Apr 2020 23:58:54: 5000000 INFO @ Wed, 15 Apr 2020 23:58:56: 8000000 INFO @ Wed, 15 Apr 2020 23:59:01: 2000000 INFO @ Wed, 15 Apr 2020 23:59:01: 6000000 INFO @ Wed, 15 Apr 2020 23:59:05: 9000000 INFO @ Wed, 15 Apr 2020 23:59:09: 3000000 INFO @ Wed, 15 Apr 2020 23:59:09: 7000000 INFO @ Wed, 15 Apr 2020 23:59:13: 10000000 INFO @ Wed, 15 Apr 2020 23:59:17: 4000000 INFO @ Wed, 15 Apr 2020 23:59:17: 8000000 INFO @ Wed, 15 Apr 2020 23:59:22: 11000000 INFO @ Wed, 15 Apr 2020 23:59:24: 5000000 INFO @ Wed, 15 Apr 2020 23:59:25: 9000000 INFO @ Wed, 15 Apr 2020 23:59:31: 12000000 INFO @ Wed, 15 Apr 2020 23:59:32: 6000000 INFO @ Wed, 15 Apr 2020 23:59:32: 10000000 INFO @ Wed, 15 Apr 2020 23:59:39: 13000000 INFO @ Wed, 15 Apr 2020 23:59:40: 7000000 INFO @ Wed, 15 Apr 2020 23:59:40: 11000000 INFO @ Wed, 15 Apr 2020 23:59:48: 8000000 INFO @ Wed, 15 Apr 2020 23:59:48: 12000000 INFO @ Wed, 15 Apr 2020 23:59:48: 14000000 INFO @ Wed, 15 Apr 2020 23:59:55: 9000000 INFO @ Wed, 15 Apr 2020 23:59:55: 13000000 INFO @ Wed, 15 Apr 2020 23:59:57: 15000000 INFO @ Thu, 16 Apr 2020 00:00:03: 10000000 INFO @ Thu, 16 Apr 2020 00:00:03: 14000000 INFO @ Thu, 16 Apr 2020 00:00:06: 16000000 INFO @ Thu, 16 Apr 2020 00:00:10: 11000000 INFO @ Thu, 16 Apr 2020 00:00:11: 15000000 INFO @ Thu, 16 Apr 2020 00:00:15: 17000000 INFO @ Thu, 16 Apr 2020 00:00:18: 12000000 INFO @ Thu, 16 Apr 2020 00:00:19: 16000000 INFO @ Thu, 16 Apr 2020 00:00:24: 18000000 INFO @ Thu, 16 Apr 2020 00:00:26: 13000000 INFO @ Thu, 16 Apr 2020 00:00:27: 17000000 INFO @ Thu, 16 Apr 2020 00:00:33: 19000000 INFO @ Thu, 16 Apr 2020 00:00:34: 14000000 INFO @ Thu, 16 Apr 2020 00:00:35: 18000000 INFO @ Thu, 16 Apr 2020 00:00:41: 20000000 INFO @ Thu, 16 Apr 2020 00:00:42: 15000000 INFO @ Thu, 16 Apr 2020 00:00:42: 19000000 INFO @ Thu, 16 Apr 2020 00:00:49: 16000000 INFO @ Thu, 16 Apr 2020 00:00:50: 20000000 INFO @ Thu, 16 Apr 2020 00:00:50: 21000000 INFO @ Thu, 16 Apr 2020 00:00:57: 17000000 INFO @ Thu, 16 Apr 2020 00:00:58: 21000000 INFO @ Thu, 16 Apr 2020 00:00:59: 22000000 INFO @ Thu, 16 Apr 2020 00:01:05: 18000000 INFO @ Thu, 16 Apr 2020 00:01:06: 22000000 INFO @ Thu, 16 Apr 2020 00:01:08: 23000000 INFO @ Thu, 16 Apr 2020 00:01:13: 19000000 INFO @ Thu, 16 Apr 2020 00:01:13: 23000000 INFO @ Thu, 16 Apr 2020 00:01:16: 24000000 INFO @ Thu, 16 Apr 2020 00:01:20: 20000000 INFO @ Thu, 16 Apr 2020 00:01:21: 24000000 INFO @ Thu, 16 Apr 2020 00:01:25: 25000000 INFO @ Thu, 16 Apr 2020 00:01:28: 21000000 INFO @ Thu, 16 Apr 2020 00:01:29: 25000000 INFO @ Thu, 16 Apr 2020 00:01:33: 26000000 INFO @ Thu, 16 Apr 2020 00:01:36: 22000000 INFO @ Thu, 16 Apr 2020 00:01:36: 26000000 INFO @ Thu, 16 Apr 2020 00:01:42: 27000000 INFO @ Thu, 16 Apr 2020 00:01:43: 23000000 INFO @ Thu, 16 Apr 2020 00:01:44: 27000000 INFO @ Thu, 16 Apr 2020 00:01:51: 28000000 INFO @ Thu, 16 Apr 2020 00:01:51: 24000000 INFO @ Thu, 16 Apr 2020 00:01:52: 28000000 INFO @ Thu, 16 Apr 2020 00:01:58: 25000000 INFO @ Thu, 16 Apr 2020 00:01:59: 29000000 INFO @ Thu, 16 Apr 2020 00:02:00: 29000000 INFO @ Thu, 16 Apr 2020 00:02:06: 26000000 INFO @ Thu, 16 Apr 2020 00:02:08: 30000000 INFO @ Thu, 16 Apr 2020 00:02:08: 30000000 INFO @ Thu, 16 Apr 2020 00:02:13: 27000000 INFO @ Thu, 16 Apr 2020 00:02:16: 31000000 INFO @ Thu, 16 Apr 2020 00:02:17: 31000000 INFO @ Thu, 16 Apr 2020 00:02:21: 28000000 INFO @ Thu, 16 Apr 2020 00:02:25: 32000000 INFO @ Thu, 16 Apr 2020 00:02:26: 32000000 INFO @ Thu, 16 Apr 2020 00:02:28: 29000000 INFO @ Thu, 16 Apr 2020 00:02:34: 33000000 INFO @ Thu, 16 Apr 2020 00:02:34: 33000000 INFO @ Thu, 16 Apr 2020 00:02:36: 30000000 INFO @ Thu, 16 Apr 2020 00:02:42: 34000000 INFO @ Thu, 16 Apr 2020 00:02:43: 34000000 INFO @ Thu, 16 Apr 2020 00:02:43: 31000000 INFO @ Thu, 16 Apr 2020 00:02:50: 32000000 INFO @ Thu, 16 Apr 2020 00:02:51: 35000000 INFO @ Thu, 16 Apr 2020 00:02:52: 35000000 INFO @ Thu, 16 Apr 2020 00:02:58: 33000000 INFO @ Thu, 16 Apr 2020 00:02:59: 36000000 INFO @ Thu, 16 Apr 2020 00:03:00: 36000000 INFO @ Thu, 16 Apr 2020 00:03:05: 34000000 INFO @ Thu, 16 Apr 2020 00:03:08: 37000000 INFO @ Thu, 16 Apr 2020 00:03:09: 37000000 INFO @ Thu, 16 Apr 2020 00:03:13: 35000000 INFO @ Thu, 16 Apr 2020 00:03:17: 38000000 INFO @ Thu, 16 Apr 2020 00:03:17: 38000000 INFO @ Thu, 16 Apr 2020 00:03:21: 36000000 INFO @ Thu, 16 Apr 2020 00:03:25: 39000000 INFO @ Thu, 16 Apr 2020 00:03:26: 39000000 INFO @ Thu, 16 Apr 2020 00:03:28: 37000000 INFO @ Thu, 16 Apr 2020 00:03:34: 40000000 INFO @ Thu, 16 Apr 2020 00:03:35: 40000000 INFO @ Thu, 16 Apr 2020 00:03:36: 38000000 INFO @ Thu, 16 Apr 2020 00:03:43: 41000000 INFO @ Thu, 16 Apr 2020 00:03:43: 39000000 INFO @ Thu, 16 Apr 2020 00:03:44: 41000000 INFO @ Thu, 16 Apr 2020 00:03:50: 40000000 INFO @ Thu, 16 Apr 2020 00:03:51: 42000000 INFO @ Thu, 16 Apr 2020 00:03:52: 42000000 INFO @ Thu, 16 Apr 2020 00:03:58: 41000000 INFO @ Thu, 16 Apr 2020 00:04:00: 43000000 INFO @ Thu, 16 Apr 2020 00:04:01: 43000000 INFO @ Thu, 16 Apr 2020 00:04:05: 42000000 INFO @ Thu, 16 Apr 2020 00:04:09: 44000000 INFO @ Thu, 16 Apr 2020 00:04:09: 44000000 INFO @ Thu, 16 Apr 2020 00:04:13: 43000000 INFO @ Thu, 16 Apr 2020 00:04:17: 45000000 INFO @ Thu, 16 Apr 2020 00:04:18: 45000000 INFO @ Thu, 16 Apr 2020 00:04:20: 44000000 INFO @ Thu, 16 Apr 2020 00:04:26: 46000000 INFO @ Thu, 16 Apr 2020 00:04:27: 46000000 INFO @ Thu, 16 Apr 2020 00:04:27: 45000000 INFO @ Thu, 16 Apr 2020 00:04:34: 47000000 INFO @ Thu, 16 Apr 2020 00:04:35: 46000000 INFO @ Thu, 16 Apr 2020 00:04:35: 47000000 INFO @ Thu, 16 Apr 2020 00:04:42: 47000000 INFO @ Thu, 16 Apr 2020 00:04:43: 48000000 INFO @ Thu, 16 Apr 2020 00:04:44: 48000000 INFO @ Thu, 16 Apr 2020 00:04:50: 48000000 INFO @ Thu, 16 Apr 2020 00:04:52: 49000000 INFO @ Thu, 16 Apr 2020 00:04:52: 49000000 INFO @ Thu, 16 Apr 2020 00:04:57: 49000000 INFO @ Thu, 16 Apr 2020 00:05:00: 50000000 INFO @ Thu, 16 Apr 2020 00:05:01: 50000000 INFO @ Thu, 16 Apr 2020 00:05:04: 50000000 INFO @ Thu, 16 Apr 2020 00:05:09: 51000000 INFO @ Thu, 16 Apr 2020 00:05:09: 51000000 INFO @ Thu, 16 Apr 2020 00:05:12: 51000000 INFO @ Thu, 16 Apr 2020 00:05:17: 52000000 INFO @ Thu, 16 Apr 2020 00:05:18: 52000000 INFO @ Thu, 16 Apr 2020 00:05:19: 52000000 INFO @ Thu, 16 Apr 2020 00:05:25: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:05:25: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:05:25: #1 total tags in treatment: 19510659 INFO @ Thu, 16 Apr 2020 00:05:25: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:05:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:05:26: #1 tags after filtering in treatment: 16212750 INFO @ Thu, 16 Apr 2020 00:05:26: #1 Redundant rate of treatment: 0.17 INFO @ Thu, 16 Apr 2020 00:05:26: #1 finished! INFO @ Thu, 16 Apr 2020 00:05:26: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:05:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:05:26: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:05:26: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:05:26: #1 total tags in treatment: 19510659 INFO @ Thu, 16 Apr 2020 00:05:26: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:05:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:05:26: #1 tags after filtering in treatment: 16212750 INFO @ Thu, 16 Apr 2020 00:05:26: #1 Redundant rate of treatment: 0.17 INFO @ Thu, 16 Apr 2020 00:05:26: #1 finished! INFO @ Thu, 16 Apr 2020 00:05:26: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:05:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:05:27: #2 number of paired peaks: 304 WARNING @ Thu, 16 Apr 2020 00:05:27: Fewer paired peaks (304) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 304 pairs to build model! INFO @ Thu, 16 Apr 2020 00:05:27: start model_add_line... INFO @ Thu, 16 Apr 2020 00:05:27: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:05:27: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:05:27: #1 total tags in treatment: 19510659 INFO @ Thu, 16 Apr 2020 00:05:27: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:05:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:05:27: start X-correlation... INFO @ Thu, 16 Apr 2020 00:05:27: end of X-cor INFO @ Thu, 16 Apr 2020 00:05:27: #2 finished! INFO @ Thu, 16 Apr 2020 00:05:27: #2 predicted fragment length is 216 bps INFO @ Thu, 16 Apr 2020 00:05:27: #2 alternative fragment length(s) may be 216 bps INFO @ Thu, 16 Apr 2020 00:05:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.05_model.r WARNING @ Thu, 16 Apr 2020 00:05:27: #2 Since the d (216) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:05:27: #2 You may need to consider one of the other alternative d(s): 216 WARNING @ Thu, 16 Apr 2020 00:05:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:05:27: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:05:27: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:05:27: #1 tags after filtering in treatment: 16212750 INFO @ Thu, 16 Apr 2020 00:05:27: #1 Redundant rate of treatment: 0.17 INFO @ Thu, 16 Apr 2020 00:05:27: #1 finished! INFO @ Thu, 16 Apr 2020 00:05:27: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:05:27: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:05:27: #2 number of paired peaks: 304 WARNING @ Thu, 16 Apr 2020 00:05:27: Fewer paired peaks (304) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 304 pairs to build model! INFO @ Thu, 16 Apr 2020 00:05:27: start model_add_line... INFO @ Thu, 16 Apr 2020 00:05:27: start X-correlation... INFO @ Thu, 16 Apr 2020 00:05:27: end of X-cor INFO @ Thu, 16 Apr 2020 00:05:27: #2 finished! INFO @ Thu, 16 Apr 2020 00:05:27: #2 predicted fragment length is 216 bps INFO @ Thu, 16 Apr 2020 00:05:27: #2 alternative fragment length(s) may be 216 bps INFO @ Thu, 16 Apr 2020 00:05:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.10_model.r WARNING @ Thu, 16 Apr 2020 00:05:27: #2 Since the d (216) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:05:27: #2 You may need to consider one of the other alternative d(s): 216 WARNING @ Thu, 16 Apr 2020 00:05:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:05:27: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:05:27: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:05:28: #2 number of paired peaks: 304 WARNING @ Thu, 16 Apr 2020 00:05:28: Fewer paired peaks (304) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 304 pairs to build model! INFO @ Thu, 16 Apr 2020 00:05:28: start model_add_line... INFO @ Thu, 16 Apr 2020 00:05:28: start X-correlation... INFO @ Thu, 16 Apr 2020 00:05:28: end of X-cor INFO @ Thu, 16 Apr 2020 00:05:28: #2 finished! INFO @ Thu, 16 Apr 2020 00:05:28: #2 predicted fragment length is 216 bps INFO @ Thu, 16 Apr 2020 00:05:28: #2 alternative fragment length(s) may be 216 bps INFO @ Thu, 16 Apr 2020 00:05:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.20_model.r WARNING @ Thu, 16 Apr 2020 00:05:28: #2 Since the d (216) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:05:28: #2 You may need to consider one of the other alternative d(s): 216 WARNING @ Thu, 16 Apr 2020 00:05:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:05:28: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:05:28: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 00:05:59: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:06:00: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:06:01: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:06:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:06:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:06:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:06:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.20_summits.bed INFO @ Thu, 16 Apr 2020 00:06:15: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (1358 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:06:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:06:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.05_summits.bed INFO @ Thu, 16 Apr 2020 00:06:16: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (25538 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:06:18: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:06:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:06:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619589/SRX6619589.10_summits.bed INFO @ Thu, 16 Apr 2020 00:06:18: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (11521 records, 4 fields): 10 millis CompletedMACS2peakCalling BigWig に変換しました。