Job ID = 5720203 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 40,085,238 reads read : 80,170,476 reads written : 80,170,476 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:29:01 40085238 reads; of these: 40085238 (100.00%) were paired; of these: 26022981 (64.92%) aligned concordantly 0 times 11649699 (29.06%) aligned concordantly exactly 1 time 2412558 (6.02%) aligned concordantly >1 times ---- 26022981 pairs aligned concordantly 0 times; of these: 11338287 (43.57%) aligned discordantly 1 time ---- 14684694 pairs aligned 0 times concordantly or discordantly; of these: 29369388 mates make up the pairs; of these: 26465210 (90.11%) aligned 0 times 1037635 (3.53%) aligned exactly 1 time 1866543 (6.36%) aligned >1 times 66.99% overall alignment rate Time searching: 01:29:01 Overall time: 01:29:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3762297 / 24879899 = 0.1512 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:32:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:32:21: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:32:21: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:32:30: 1000000 INFO @ Thu, 16 Apr 2020 00:32:38: 2000000 INFO @ Thu, 16 Apr 2020 00:32:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:32:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:32:51: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:32:51: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:32:55: 4000000 INFO @ Thu, 16 Apr 2020 00:33:01: 1000000 INFO @ Thu, 16 Apr 2020 00:33:05: 5000000 INFO @ Thu, 16 Apr 2020 00:33:10: 2000000 INFO @ Thu, 16 Apr 2020 00:33:14: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:33:20: 3000000 INFO @ Thu, 16 Apr 2020 00:33:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:33:21: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:33:21: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:33:23: 7000000 INFO @ Thu, 16 Apr 2020 00:33:29: 4000000 INFO @ Thu, 16 Apr 2020 00:33:32: 1000000 INFO @ Thu, 16 Apr 2020 00:33:33: 8000000 INFO @ Thu, 16 Apr 2020 00:33:39: 5000000 INFO @ Thu, 16 Apr 2020 00:33:41: 2000000 INFO @ Thu, 16 Apr 2020 00:33:43: 9000000 INFO @ Thu, 16 Apr 2020 00:33:49: 6000000 INFO @ Thu, 16 Apr 2020 00:33:51: 3000000 INFO @ Thu, 16 Apr 2020 00:33:52: 10000000 INFO @ Thu, 16 Apr 2020 00:33:58: 7000000 INFO @ Thu, 16 Apr 2020 00:34:01: 4000000 INFO @ Thu, 16 Apr 2020 00:34:02: 11000000 INFO @ Thu, 16 Apr 2020 00:34:08: 8000000 INFO @ Thu, 16 Apr 2020 00:34:11: 5000000 INFO @ Thu, 16 Apr 2020 00:34:12: 12000000 INFO @ Thu, 16 Apr 2020 00:34:17: 9000000 INFO @ Thu, 16 Apr 2020 00:34:20: 6000000 INFO @ Thu, 16 Apr 2020 00:34:21: 13000000 INFO @ Thu, 16 Apr 2020 00:34:27: 10000000 INFO @ Thu, 16 Apr 2020 00:34:30: 7000000 INFO @ Thu, 16 Apr 2020 00:34:31: 14000000 INFO @ Thu, 16 Apr 2020 00:34:37: 11000000 INFO @ Thu, 16 Apr 2020 00:34:40: 8000000 INFO @ Thu, 16 Apr 2020 00:34:41: 15000000 INFO @ Thu, 16 Apr 2020 00:34:47: 12000000 INFO @ Thu, 16 Apr 2020 00:34:49: 9000000 INFO @ Thu, 16 Apr 2020 00:34:51: 16000000 INFO @ Thu, 16 Apr 2020 00:34:56: 13000000 INFO @ Thu, 16 Apr 2020 00:34:59: 10000000 INFO @ Thu, 16 Apr 2020 00:35:01: 17000000 INFO @ Thu, 16 Apr 2020 00:35:06: 14000000 INFO @ Thu, 16 Apr 2020 00:35:09: 11000000 INFO @ Thu, 16 Apr 2020 00:35:10: 18000000 INFO @ Thu, 16 Apr 2020 00:35:16: 15000000 INFO @ Thu, 16 Apr 2020 00:35:18: 12000000 INFO @ Thu, 16 Apr 2020 00:35:20: 19000000 INFO @ Thu, 16 Apr 2020 00:35:25: 16000000 INFO @ Thu, 16 Apr 2020 00:35:28: 13000000 INFO @ Thu, 16 Apr 2020 00:35:30: 20000000 INFO @ Thu, 16 Apr 2020 00:35:35: 17000000 INFO @ Thu, 16 Apr 2020 00:35:38: 14000000 INFO @ Thu, 16 Apr 2020 00:35:40: 21000000 INFO @ Thu, 16 Apr 2020 00:35:45: 18000000 INFO @ Thu, 16 Apr 2020 00:35:48: 15000000 INFO @ Thu, 16 Apr 2020 00:35:50: 22000000 INFO @ Thu, 16 Apr 2020 00:35:55: 19000000 INFO @ Thu, 16 Apr 2020 00:35:58: 16000000 INFO @ Thu, 16 Apr 2020 00:35:59: 23000000 INFO @ Thu, 16 Apr 2020 00:36:05: 20000000 INFO @ Thu, 16 Apr 2020 00:36:07: 17000000 INFO @ Thu, 16 Apr 2020 00:36:09: 24000000 INFO @ Thu, 16 Apr 2020 00:36:15: 21000000 INFO @ Thu, 16 Apr 2020 00:36:17: 18000000 INFO @ Thu, 16 Apr 2020 00:36:19: 25000000 INFO @ Thu, 16 Apr 2020 00:36:25: 22000000 INFO @ Thu, 16 Apr 2020 00:36:27: 19000000 INFO @ Thu, 16 Apr 2020 00:36:29: 26000000 INFO @ Thu, 16 Apr 2020 00:36:34: 23000000 INFO @ Thu, 16 Apr 2020 00:36:37: 20000000 INFO @ Thu, 16 Apr 2020 00:36:39: 27000000 INFO @ Thu, 16 Apr 2020 00:36:44: 24000000 INFO @ Thu, 16 Apr 2020 00:36:46: 21000000 INFO @ Thu, 16 Apr 2020 00:36:49: 28000000 INFO @ Thu, 16 Apr 2020 00:36:54: 25000000 INFO @ Thu, 16 Apr 2020 00:36:56: 22000000 INFO @ Thu, 16 Apr 2020 00:36:59: 29000000 INFO @ Thu, 16 Apr 2020 00:37:04: 26000000 INFO @ Thu, 16 Apr 2020 00:37:06: 23000000 INFO @ Thu, 16 Apr 2020 00:37:08: 30000000 INFO @ Thu, 16 Apr 2020 00:37:14: 27000000 INFO @ Thu, 16 Apr 2020 00:37:16: 24000000 INFO @ Thu, 16 Apr 2020 00:37:18: 31000000 INFO @ Thu, 16 Apr 2020 00:37:24: 28000000 INFO @ Thu, 16 Apr 2020 00:37:25: 25000000 INFO @ Thu, 16 Apr 2020 00:37:27: 32000000 INFO @ Thu, 16 Apr 2020 00:37:33: 29000000 INFO @ Thu, 16 Apr 2020 00:37:35: 26000000 INFO @ Thu, 16 Apr 2020 00:37:37: 33000000 INFO @ Thu, 16 Apr 2020 00:37:43: 30000000 INFO @ Thu, 16 Apr 2020 00:37:45: 27000000 INFO @ Thu, 16 Apr 2020 00:37:47: 34000000 INFO @ Thu, 16 Apr 2020 00:37:53: 31000000 INFO @ Thu, 16 Apr 2020 00:37:55: 28000000 INFO @ Thu, 16 Apr 2020 00:37:56: 35000000 INFO @ Thu, 16 Apr 2020 00:38:02: 32000000 INFO @ Thu, 16 Apr 2020 00:38:05: 29000000 INFO @ Thu, 16 Apr 2020 00:38:06: 36000000 INFO @ Thu, 16 Apr 2020 00:38:12: 33000000 INFO @ Thu, 16 Apr 2020 00:38:14: 30000000 INFO @ Thu, 16 Apr 2020 00:38:16: 37000000 INFO @ Thu, 16 Apr 2020 00:38:22: 34000000 INFO @ Thu, 16 Apr 2020 00:38:24: 31000000 INFO @ Thu, 16 Apr 2020 00:38:25: 38000000 INFO @ Thu, 16 Apr 2020 00:38:31: 35000000 INFO @ Thu, 16 Apr 2020 00:38:34: 32000000 INFO @ Thu, 16 Apr 2020 00:38:35: 39000000 INFO @ Thu, 16 Apr 2020 00:38:41: 36000000 INFO @ Thu, 16 Apr 2020 00:38:43: 33000000 INFO @ Thu, 16 Apr 2020 00:38:45: 40000000 INFO @ Thu, 16 Apr 2020 00:38:50: 37000000 INFO @ Thu, 16 Apr 2020 00:38:52: 34000000 INFO @ Thu, 16 Apr 2020 00:38:54: 41000000 INFO @ Thu, 16 Apr 2020 00:39:00: 38000000 INFO @ Thu, 16 Apr 2020 00:39:02: 35000000 INFO @ Thu, 16 Apr 2020 00:39:04: 42000000 INFO @ Thu, 16 Apr 2020 00:39:10: 39000000 INFO @ Thu, 16 Apr 2020 00:39:12: 36000000 INFO @ Thu, 16 Apr 2020 00:39:14: 43000000 INFO @ Thu, 16 Apr 2020 00:39:20: 40000000 INFO @ Thu, 16 Apr 2020 00:39:21: 37000000 INFO @ Thu, 16 Apr 2020 00:39:23: 44000000 INFO @ Thu, 16 Apr 2020 00:39:29: 41000000 INFO @ Thu, 16 Apr 2020 00:39:31: 38000000 INFO @ Thu, 16 Apr 2020 00:39:33: 45000000 INFO @ Thu, 16 Apr 2020 00:39:39: 42000000 INFO @ Thu, 16 Apr 2020 00:39:41: 39000000 INFO @ Thu, 16 Apr 2020 00:39:43: 46000000 INFO @ Thu, 16 Apr 2020 00:39:44: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:39:44: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:39:44: #1 total tags in treatment: 11761712 INFO @ Thu, 16 Apr 2020 00:39:44: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:39:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:39:45: #1 tags after filtering in treatment: 10058363 INFO @ Thu, 16 Apr 2020 00:39:45: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:39:45: #1 finished! INFO @ Thu, 16 Apr 2020 00:39:45: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:39:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:39:45: #2 number of paired peaks: 300 WARNING @ Thu, 16 Apr 2020 00:39:45: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 16 Apr 2020 00:39:45: start model_add_line... INFO @ Thu, 16 Apr 2020 00:39:45: start X-correlation... INFO @ Thu, 16 Apr 2020 00:39:46: end of X-cor INFO @ Thu, 16 Apr 2020 00:39:46: #2 finished! INFO @ Thu, 16 Apr 2020 00:39:46: #2 predicted fragment length is 219 bps INFO @ Thu, 16 Apr 2020 00:39:46: #2 alternative fragment length(s) may be 219 bps INFO @ Thu, 16 Apr 2020 00:39:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.05_model.r WARNING @ Thu, 16 Apr 2020 00:39:46: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:39:46: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Thu, 16 Apr 2020 00:39:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:39:46: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:39:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:39:48: 43000000 INFO @ Thu, 16 Apr 2020 00:39:50: 40000000 INFO @ Thu, 16 Apr 2020 00:39:57: 44000000 INFO @ Thu, 16 Apr 2020 00:39:59: 41000000 INFO @ Thu, 16 Apr 2020 00:40:06: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:40:06: 45000000 INFO @ Thu, 16 Apr 2020 00:40:08: 42000000 INFO @ Thu, 16 Apr 2020 00:40:15: 46000000 INFO @ Thu, 16 Apr 2020 00:40:16: 43000000 INFO @ Thu, 16 Apr 2020 00:40:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:40:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:40:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.05_summits.bed INFO @ Thu, 16 Apr 2020 00:40:16: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (489 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:40:17: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:40:17: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:40:17: #1 total tags in treatment: 11761712 INFO @ Thu, 16 Apr 2020 00:40:17: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:40:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:40:17: #1 tags after filtering in treatment: 10058363 INFO @ Thu, 16 Apr 2020 00:40:17: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:40:17: #1 finished! INFO @ Thu, 16 Apr 2020 00:40:17: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:40:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:40:18: #2 number of paired peaks: 300 WARNING @ Thu, 16 Apr 2020 00:40:18: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 16 Apr 2020 00:40:18: start model_add_line... INFO @ Thu, 16 Apr 2020 00:40:18: start X-correlation... INFO @ Thu, 16 Apr 2020 00:40:18: end of X-cor INFO @ Thu, 16 Apr 2020 00:40:18: #2 finished! INFO @ Thu, 16 Apr 2020 00:40:18: #2 predicted fragment length is 219 bps INFO @ Thu, 16 Apr 2020 00:40:18: #2 alternative fragment length(s) may be 219 bps INFO @ Thu, 16 Apr 2020 00:40:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.10_model.r WARNING @ Thu, 16 Apr 2020 00:40:18: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:40:18: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Thu, 16 Apr 2020 00:40:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:40:18: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:40:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:40:24: 44000000 INFO @ Thu, 16 Apr 2020 00:40:33: 45000000 INFO @ Thu, 16 Apr 2020 00:40:38: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:40:41: 46000000 INFO @ Thu, 16 Apr 2020 00:40:42: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:40:42: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:40:42: #1 total tags in treatment: 11761712 INFO @ Thu, 16 Apr 2020 00:40:42: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:40:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:40:43: #1 tags after filtering in treatment: 10058363 INFO @ Thu, 16 Apr 2020 00:40:43: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:40:43: #1 finished! INFO @ Thu, 16 Apr 2020 00:40:43: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:40:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:40:43: #2 number of paired peaks: 300 WARNING @ Thu, 16 Apr 2020 00:40:43: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 16 Apr 2020 00:40:43: start model_add_line... INFO @ Thu, 16 Apr 2020 00:40:43: start X-correlation... INFO @ Thu, 16 Apr 2020 00:40:43: end of X-cor INFO @ Thu, 16 Apr 2020 00:40:43: #2 finished! INFO @ Thu, 16 Apr 2020 00:40:43: #2 predicted fragment length is 219 bps INFO @ Thu, 16 Apr 2020 00:40:43: #2 alternative fragment length(s) may be 219 bps INFO @ Thu, 16 Apr 2020 00:40:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.20_model.r WARNING @ Thu, 16 Apr 2020 00:40:43: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:40:43: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Thu, 16 Apr 2020 00:40:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:40:43: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:40:43: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:40:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:40:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:40:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.10_summits.bed INFO @ Thu, 16 Apr 2020 00:40:49: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (335 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:41:04: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:41:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:41:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:41:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619572/SRX6619572.20_summits.bed INFO @ Thu, 16 Apr 2020 00:41:14: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (222 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。