Job ID = 5720201 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:30:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading inform004C) ) 2020-04-15T12:30:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading inform004C) ) 2020-04-15T12:30:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading inform004C) ) 2020-04-15T12:30:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:07:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:08:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:17:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 40,085,238 reads read : 80,170,476 reads written : 80,170,476 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:01:01 40085238 reads; of these: 40085238 (100.00%) were paired; of these: 26022981 (64.92%) aligned concordantly 0 times 11649699 (29.06%) aligned concordantly exactly 1 time 2412558 (6.02%) aligned concordantly >1 times ---- 26022981 pairs aligned concordantly 0 times; of these: 11338287 (43.57%) aligned discordantly 1 time ---- 14684694 pairs aligned 0 times concordantly or discordantly; of these: 29369388 mates make up the pairs; of these: 26465204 (90.11%) aligned 0 times 1037639 (3.53%) aligned exactly 1 time 1866545 (6.36%) aligned >1 times 66.99% overall alignment rate Time searching: 01:01:01 Overall time: 01:01:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3762297 / 24879899 = 0.1512 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:59:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:59:35: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:59:35: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:59:42: 1000000 INFO @ Wed, 15 Apr 2020 23:59:49: 2000000 INFO @ Wed, 15 Apr 2020 23:59:55: 3000000 INFO @ Thu, 16 Apr 2020 00:00:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:00:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:00:05: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:00:05: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:00:07: 5000000 INFO @ Thu, 16 Apr 2020 00:00:11: 1000000 INFO @ Thu, 16 Apr 2020 00:00:13: 6000000 INFO @ Thu, 16 Apr 2020 00:00:17: 2000000 INFO @ Thu, 16 Apr 2020 00:00:19: 7000000 INFO @ Thu, 16 Apr 2020 00:00:23: 3000000 INFO @ Thu, 16 Apr 2020 00:00:26: 8000000 INFO @ Thu, 16 Apr 2020 00:00:29: 4000000 INFO @ Thu, 16 Apr 2020 00:00:32: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:00:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:00:35: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:00:35: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:00:35: 5000000 INFO @ Thu, 16 Apr 2020 00:00:38: 10000000 INFO @ Thu, 16 Apr 2020 00:00:41: 1000000 INFO @ Thu, 16 Apr 2020 00:00:41: 6000000 INFO @ Thu, 16 Apr 2020 00:00:44: 11000000 INFO @ Thu, 16 Apr 2020 00:00:47: 2000000 INFO @ Thu, 16 Apr 2020 00:00:47: 7000000 INFO @ Thu, 16 Apr 2020 00:00:50: 12000000 INFO @ Thu, 16 Apr 2020 00:00:53: 3000000 INFO @ Thu, 16 Apr 2020 00:00:53: 8000000 INFO @ Thu, 16 Apr 2020 00:00:56: 13000000 INFO @ Thu, 16 Apr 2020 00:00:59: 4000000 INFO @ Thu, 16 Apr 2020 00:00:59: 9000000 INFO @ Thu, 16 Apr 2020 00:01:02: 14000000 INFO @ Thu, 16 Apr 2020 00:01:05: 5000000 INFO @ Thu, 16 Apr 2020 00:01:06: 10000000 INFO @ Thu, 16 Apr 2020 00:01:09: 15000000 INFO @ Thu, 16 Apr 2020 00:01:11: 6000000 INFO @ Thu, 16 Apr 2020 00:01:12: 11000000 INFO @ Thu, 16 Apr 2020 00:01:15: 16000000 INFO @ Thu, 16 Apr 2020 00:01:18: 7000000 INFO @ Thu, 16 Apr 2020 00:01:18: 12000000 INFO @ Thu, 16 Apr 2020 00:01:21: 17000000 INFO @ Thu, 16 Apr 2020 00:01:24: 8000000 INFO @ Thu, 16 Apr 2020 00:01:24: 13000000 INFO @ Thu, 16 Apr 2020 00:01:27: 18000000 INFO @ Thu, 16 Apr 2020 00:01:30: 9000000 INFO @ Thu, 16 Apr 2020 00:01:30: 14000000 INFO @ Thu, 16 Apr 2020 00:01:33: 19000000 INFO @ Thu, 16 Apr 2020 00:01:36: 10000000 INFO @ Thu, 16 Apr 2020 00:01:36: 15000000 INFO @ Thu, 16 Apr 2020 00:01:39: 20000000 INFO @ Thu, 16 Apr 2020 00:01:42: 11000000 INFO @ Thu, 16 Apr 2020 00:01:42: 16000000 INFO @ Thu, 16 Apr 2020 00:01:46: 21000000 INFO @ Thu, 16 Apr 2020 00:01:48: 12000000 INFO @ Thu, 16 Apr 2020 00:01:49: 17000000 INFO @ Thu, 16 Apr 2020 00:01:52: 22000000 INFO @ Thu, 16 Apr 2020 00:01:55: 13000000 INFO @ Thu, 16 Apr 2020 00:01:55: 18000000 INFO @ Thu, 16 Apr 2020 00:01:58: 23000000 INFO @ Thu, 16 Apr 2020 00:02:01: 14000000 INFO @ Thu, 16 Apr 2020 00:02:01: 19000000 INFO @ Thu, 16 Apr 2020 00:02:04: 24000000 INFO @ Thu, 16 Apr 2020 00:02:07: 15000000 INFO @ Thu, 16 Apr 2020 00:02:07: 20000000 INFO @ Thu, 16 Apr 2020 00:02:10: 25000000 INFO @ Thu, 16 Apr 2020 00:02:13: 16000000 INFO @ Thu, 16 Apr 2020 00:02:13: 21000000 INFO @ Thu, 16 Apr 2020 00:02:16: 26000000 INFO @ Thu, 16 Apr 2020 00:02:19: 17000000 INFO @ Thu, 16 Apr 2020 00:02:19: 22000000 INFO @ Thu, 16 Apr 2020 00:02:22: 27000000 INFO @ Thu, 16 Apr 2020 00:02:25: 18000000 INFO @ Thu, 16 Apr 2020 00:02:25: 23000000 INFO @ Thu, 16 Apr 2020 00:02:28: 28000000 INFO @ Thu, 16 Apr 2020 00:02:31: 19000000 INFO @ Thu, 16 Apr 2020 00:02:31: 24000000 INFO @ Thu, 16 Apr 2020 00:02:35: 29000000 INFO @ Thu, 16 Apr 2020 00:02:37: 20000000 INFO @ Thu, 16 Apr 2020 00:02:37: 25000000 INFO @ Thu, 16 Apr 2020 00:02:41: 30000000 INFO @ Thu, 16 Apr 2020 00:02:43: 21000000 INFO @ Thu, 16 Apr 2020 00:02:43: 26000000 INFO @ Thu, 16 Apr 2020 00:02:47: 31000000 INFO @ Thu, 16 Apr 2020 00:02:49: 22000000 INFO @ Thu, 16 Apr 2020 00:02:49: 27000000 INFO @ Thu, 16 Apr 2020 00:02:52: 32000000 INFO @ Thu, 16 Apr 2020 00:02:55: 23000000 INFO @ Thu, 16 Apr 2020 00:02:55: 28000000 INFO @ Thu, 16 Apr 2020 00:02:58: 33000000 INFO @ Thu, 16 Apr 2020 00:03:01: 24000000 INFO @ Thu, 16 Apr 2020 00:03:01: 29000000 INFO @ Thu, 16 Apr 2020 00:03:04: 34000000 INFO @ Thu, 16 Apr 2020 00:03:07: 25000000 INFO @ Thu, 16 Apr 2020 00:03:07: 30000000 INFO @ Thu, 16 Apr 2020 00:03:10: 35000000 INFO @ Thu, 16 Apr 2020 00:03:13: 26000000 INFO @ Thu, 16 Apr 2020 00:03:13: 31000000 INFO @ Thu, 16 Apr 2020 00:03:16: 36000000 INFO @ Thu, 16 Apr 2020 00:03:19: 32000000 INFO @ Thu, 16 Apr 2020 00:03:19: 27000000 INFO @ Thu, 16 Apr 2020 00:03:22: 37000000 INFO @ Thu, 16 Apr 2020 00:03:25: 33000000 INFO @ Thu, 16 Apr 2020 00:03:25: 28000000 INFO @ Thu, 16 Apr 2020 00:03:28: 38000000 INFO @ Thu, 16 Apr 2020 00:03:31: 34000000 INFO @ Thu, 16 Apr 2020 00:03:31: 29000000 INFO @ Thu, 16 Apr 2020 00:03:34: 39000000 INFO @ Thu, 16 Apr 2020 00:03:36: 35000000 INFO @ Thu, 16 Apr 2020 00:03:37: 30000000 INFO @ Thu, 16 Apr 2020 00:03:40: 40000000 INFO @ Thu, 16 Apr 2020 00:03:42: 36000000 INFO @ Thu, 16 Apr 2020 00:03:43: 31000000 INFO @ Thu, 16 Apr 2020 00:03:46: 41000000 INFO @ Thu, 16 Apr 2020 00:03:48: 37000000 INFO @ Thu, 16 Apr 2020 00:03:49: 32000000 INFO @ Thu, 16 Apr 2020 00:03:52: 42000000 INFO @ Thu, 16 Apr 2020 00:03:54: 38000000 INFO @ Thu, 16 Apr 2020 00:03:55: 33000000 INFO @ Thu, 16 Apr 2020 00:03:58: 43000000 INFO @ Thu, 16 Apr 2020 00:04:01: 34000000 INFO @ Thu, 16 Apr 2020 00:04:01: 39000000 INFO @ Thu, 16 Apr 2020 00:04:04: 44000000 INFO @ Thu, 16 Apr 2020 00:04:06: 35000000 INFO @ Thu, 16 Apr 2020 00:04:07: 40000000 INFO @ Thu, 16 Apr 2020 00:04:10: 45000000 INFO @ Thu, 16 Apr 2020 00:04:12: 36000000 INFO @ Thu, 16 Apr 2020 00:04:12: 41000000 INFO @ Thu, 16 Apr 2020 00:04:17: 46000000 INFO @ Thu, 16 Apr 2020 00:04:18: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:04:18: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:04:18: #1 total tags in treatment: 11761712 INFO @ Thu, 16 Apr 2020 00:04:18: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:04:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:04:18: #1 tags after filtering in treatment: 10058363 INFO @ Thu, 16 Apr 2020 00:04:18: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:04:18: #1 finished! INFO @ Thu, 16 Apr 2020 00:04:18: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:04:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:04:18: 42000000 INFO @ Thu, 16 Apr 2020 00:04:19: 37000000 INFO @ Thu, 16 Apr 2020 00:04:19: #2 number of paired peaks: 300 WARNING @ Thu, 16 Apr 2020 00:04:19: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 16 Apr 2020 00:04:19: start model_add_line... INFO @ Thu, 16 Apr 2020 00:04:19: start X-correlation... INFO @ Thu, 16 Apr 2020 00:04:19: end of X-cor INFO @ Thu, 16 Apr 2020 00:04:19: #2 finished! INFO @ Thu, 16 Apr 2020 00:04:19: #2 predicted fragment length is 219 bps INFO @ Thu, 16 Apr 2020 00:04:19: #2 alternative fragment length(s) may be 219 bps INFO @ Thu, 16 Apr 2020 00:04:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.05_model.r WARNING @ Thu, 16 Apr 2020 00:04:19: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:04:19: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Thu, 16 Apr 2020 00:04:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:04:19: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:04:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:04:24: 43000000 INFO @ Thu, 16 Apr 2020 00:04:25: 38000000 INFO @ Thu, 16 Apr 2020 00:04:30: 44000000 INFO @ Thu, 16 Apr 2020 00:04:31: 39000000 INFO @ Thu, 16 Apr 2020 00:04:36: 45000000 INFO @ Thu, 16 Apr 2020 00:04:37: 40000000 INFO @ Thu, 16 Apr 2020 00:04:39: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:04:42: 46000000 INFO @ Thu, 16 Apr 2020 00:04:43: 41000000 INFO @ Thu, 16 Apr 2020 00:04:44: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:04:44: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:04:44: #1 total tags in treatment: 11761712 INFO @ Thu, 16 Apr 2020 00:04:44: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:04:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:04:44: #1 tags after filtering in treatment: 10058363 INFO @ Thu, 16 Apr 2020 00:04:44: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:04:44: #1 finished! INFO @ Thu, 16 Apr 2020 00:04:44: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:04:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:04:44: #2 number of paired peaks: 300 WARNING @ Thu, 16 Apr 2020 00:04:44: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 16 Apr 2020 00:04:44: start model_add_line... INFO @ Thu, 16 Apr 2020 00:04:44: start X-correlation... INFO @ Thu, 16 Apr 2020 00:04:44: end of X-cor INFO @ Thu, 16 Apr 2020 00:04:44: #2 finished! INFO @ Thu, 16 Apr 2020 00:04:44: #2 predicted fragment length is 219 bps INFO @ Thu, 16 Apr 2020 00:04:44: #2 alternative fragment length(s) may be 219 bps INFO @ Thu, 16 Apr 2020 00:04:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.10_model.r WARNING @ Thu, 16 Apr 2020 00:04:44: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:04:44: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Thu, 16 Apr 2020 00:04:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:04:44: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:04:44: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:04:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:04:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:04:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.05_summits.bed INFO @ Thu, 16 Apr 2020 00:04:49: Done! INFO @ Thu, 16 Apr 2020 00:04:49: 42000000 pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (489 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:04:55: 43000000 INFO @ Thu, 16 Apr 2020 00:05:00: 44000000 INFO @ Thu, 16 Apr 2020 00:05:05: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:05:06: 45000000 INFO @ Thu, 16 Apr 2020 00:05:13: 46000000 INFO @ Thu, 16 Apr 2020 00:05:14: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:05:14: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:05:14: #1 total tags in treatment: 11761712 INFO @ Thu, 16 Apr 2020 00:05:14: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:05:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:05:14: #1 tags after filtering in treatment: 10058363 INFO @ Thu, 16 Apr 2020 00:05:14: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:05:14: #1 finished! INFO @ Thu, 16 Apr 2020 00:05:14: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:05:14: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:05:15: #2 number of paired peaks: 300 WARNING @ Thu, 16 Apr 2020 00:05:15: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 16 Apr 2020 00:05:15: start model_add_line... INFO @ Thu, 16 Apr 2020 00:05:15: start X-correlation... INFO @ Thu, 16 Apr 2020 00:05:15: end of X-cor INFO @ Thu, 16 Apr 2020 00:05:15: #2 finished! INFO @ Thu, 16 Apr 2020 00:05:15: #2 predicted fragment length is 219 bps INFO @ Thu, 16 Apr 2020 00:05:15: #2 alternative fragment length(s) may be 219 bps INFO @ Thu, 16 Apr 2020 00:05:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.20_model.r WARNING @ Thu, 16 Apr 2020 00:05:15: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:05:15: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Thu, 16 Apr 2020 00:05:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:05:15: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:05:15: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:05:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:05:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:05:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.10_summits.bed INFO @ Thu, 16 Apr 2020 00:05:15: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (335 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:05:35: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:05:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:05:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:05:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619570/SRX6619570.20_summits.bed INFO @ Thu, 16 Apr 2020 00:05:45: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (222 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。