Job ID = 5720199 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:50:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:54:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:56:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:01:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.238' from '172.19.7.194' 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.238) from '172.19.7.194' spots read : 30,682,782 reads read : 61,365,564 reads written : 61,365,564 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:53:17 30682782 reads; of these: 30682782 (100.00%) were paired; of these: 10931634 (35.63%) aligned concordantly 0 times 16807203 (54.78%) aligned concordantly exactly 1 time 2943945 (9.59%) aligned concordantly >1 times ---- 10931634 pairs aligned concordantly 0 times; of these: 5599138 (51.22%) aligned discordantly 1 time ---- 5332496 pairs aligned 0 times concordantly or discordantly; of these: 10664992 mates make up the pairs; of these: 8950447 (83.92%) aligned 0 times 642239 (6.02%) aligned exactly 1 time 1072306 (10.05%) aligned >1 times 85.41% overall alignment rate Time searching: 00:53:17 Overall time: 00:53:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2738932 / 24959879 = 0.1097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:36:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:36:03: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:36:03: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:36:10: 1000000 INFO @ Wed, 15 Apr 2020 23:36:19: 2000000 INFO @ Wed, 15 Apr 2020 23:36:29: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:36:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:36:33: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:36:33: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:36:37: 4000000 INFO @ Wed, 15 Apr 2020 23:36:43: 1000000 INFO @ Wed, 15 Apr 2020 23:36:46: 5000000 INFO @ Wed, 15 Apr 2020 23:36:53: 2000000 INFO @ Wed, 15 Apr 2020 23:36:55: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:37:02: 3000000 INFO @ Wed, 15 Apr 2020 23:37:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:37:03: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:37:03: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:37:04: 7000000 INFO @ Wed, 15 Apr 2020 23:37:12: 1000000 INFO @ Wed, 15 Apr 2020 23:37:12: 4000000 INFO @ Wed, 15 Apr 2020 23:37:14: 8000000 INFO @ Wed, 15 Apr 2020 23:37:21: 2000000 INFO @ Wed, 15 Apr 2020 23:37:22: 5000000 INFO @ Wed, 15 Apr 2020 23:37:23: 9000000 INFO @ Wed, 15 Apr 2020 23:37:30: 3000000 INFO @ Wed, 15 Apr 2020 23:37:32: 10000000 INFO @ Wed, 15 Apr 2020 23:37:32: 6000000 INFO @ Wed, 15 Apr 2020 23:37:39: 4000000 INFO @ Wed, 15 Apr 2020 23:37:41: 11000000 INFO @ Wed, 15 Apr 2020 23:37:42: 7000000 INFO @ Wed, 15 Apr 2020 23:37:48: 5000000 INFO @ Wed, 15 Apr 2020 23:37:50: 12000000 INFO @ Wed, 15 Apr 2020 23:37:52: 8000000 INFO @ Wed, 15 Apr 2020 23:37:56: 6000000 INFO @ Wed, 15 Apr 2020 23:38:00: 13000000 INFO @ Wed, 15 Apr 2020 23:38:02: 9000000 INFO @ Wed, 15 Apr 2020 23:38:05: 7000000 INFO @ Wed, 15 Apr 2020 23:38:10: 14000000 INFO @ Wed, 15 Apr 2020 23:38:12: 10000000 INFO @ Wed, 15 Apr 2020 23:38:14: 8000000 INFO @ Wed, 15 Apr 2020 23:38:20: 15000000 INFO @ Wed, 15 Apr 2020 23:38:22: 11000000 INFO @ Wed, 15 Apr 2020 23:38:22: 9000000 INFO @ Wed, 15 Apr 2020 23:38:29: 16000000 INFO @ Wed, 15 Apr 2020 23:38:31: 10000000 INFO @ Wed, 15 Apr 2020 23:38:31: 12000000 INFO @ Wed, 15 Apr 2020 23:38:38: 17000000 INFO @ Wed, 15 Apr 2020 23:38:40: 11000000 INFO @ Wed, 15 Apr 2020 23:38:41: 13000000 INFO @ Wed, 15 Apr 2020 23:38:47: 18000000 INFO @ Wed, 15 Apr 2020 23:38:48: 12000000 INFO @ Wed, 15 Apr 2020 23:38:51: 14000000 INFO @ Wed, 15 Apr 2020 23:38:56: 19000000 INFO @ Wed, 15 Apr 2020 23:38:57: 13000000 INFO @ Wed, 15 Apr 2020 23:39:01: 15000000 INFO @ Wed, 15 Apr 2020 23:39:05: 20000000 INFO @ Wed, 15 Apr 2020 23:39:06: 14000000 INFO @ Wed, 15 Apr 2020 23:39:11: 16000000 INFO @ Wed, 15 Apr 2020 23:39:14: 21000000 INFO @ Wed, 15 Apr 2020 23:39:14: 15000000 INFO @ Wed, 15 Apr 2020 23:39:21: 17000000 INFO @ Wed, 15 Apr 2020 23:39:23: 22000000 INFO @ Wed, 15 Apr 2020 23:39:23: 16000000 INFO @ Wed, 15 Apr 2020 23:39:31: 18000000 INFO @ Wed, 15 Apr 2020 23:39:32: 23000000 INFO @ Wed, 15 Apr 2020 23:39:32: 17000000 INFO @ Wed, 15 Apr 2020 23:39:40: 24000000 INFO @ Wed, 15 Apr 2020 23:39:41: 18000000 INFO @ Wed, 15 Apr 2020 23:39:41: 19000000 INFO @ Wed, 15 Apr 2020 23:39:49: 19000000 INFO @ Wed, 15 Apr 2020 23:39:50: 25000000 INFO @ Wed, 15 Apr 2020 23:39:51: 20000000 INFO @ Wed, 15 Apr 2020 23:39:58: 20000000 INFO @ Wed, 15 Apr 2020 23:39:58: 26000000 INFO @ Wed, 15 Apr 2020 23:40:00: 21000000 INFO @ Wed, 15 Apr 2020 23:40:07: 27000000 INFO @ Wed, 15 Apr 2020 23:40:08: 21000000 INFO @ Wed, 15 Apr 2020 23:40:09: 22000000 INFO @ Wed, 15 Apr 2020 23:40:16: 28000000 INFO @ Wed, 15 Apr 2020 23:40:17: 22000000 INFO @ Wed, 15 Apr 2020 23:40:19: 23000000 INFO @ Wed, 15 Apr 2020 23:40:24: 29000000 INFO @ Wed, 15 Apr 2020 23:40:26: 23000000 INFO @ Wed, 15 Apr 2020 23:40:29: 24000000 INFO @ Wed, 15 Apr 2020 23:40:34: 30000000 INFO @ Wed, 15 Apr 2020 23:40:35: 24000000 INFO @ Wed, 15 Apr 2020 23:40:39: 25000000 INFO @ Wed, 15 Apr 2020 23:40:43: 25000000 INFO @ Wed, 15 Apr 2020 23:40:44: 31000000 INFO @ Wed, 15 Apr 2020 23:40:49: 26000000 INFO @ Wed, 15 Apr 2020 23:40:51: 26000000 INFO @ Wed, 15 Apr 2020 23:40:54: 32000000 INFO @ Wed, 15 Apr 2020 23:40:59: 27000000 INFO @ Wed, 15 Apr 2020 23:41:00: 27000000 INFO @ Wed, 15 Apr 2020 23:41:03: 33000000 INFO @ Wed, 15 Apr 2020 23:41:09: 28000000 INFO @ Wed, 15 Apr 2020 23:41:10: 28000000 INFO @ Wed, 15 Apr 2020 23:41:14: 34000000 INFO @ Wed, 15 Apr 2020 23:41:19: 29000000 INFO @ Wed, 15 Apr 2020 23:41:20: 29000000 INFO @ Wed, 15 Apr 2020 23:41:24: 35000000 INFO @ Wed, 15 Apr 2020 23:41:29: 30000000 INFO @ Wed, 15 Apr 2020 23:41:30: 30000000 INFO @ Wed, 15 Apr 2020 23:41:33: 36000000 INFO @ Wed, 15 Apr 2020 23:41:39: 31000000 INFO @ Wed, 15 Apr 2020 23:41:40: 31000000 INFO @ Wed, 15 Apr 2020 23:41:44: 37000000 INFO @ Wed, 15 Apr 2020 23:41:49: 32000000 INFO @ Wed, 15 Apr 2020 23:41:50: 32000000 INFO @ Wed, 15 Apr 2020 23:41:54: 38000000 INFO @ Wed, 15 Apr 2020 23:41:59: 33000000 INFO @ Wed, 15 Apr 2020 23:42:00: 33000000 INFO @ Wed, 15 Apr 2020 23:42:04: 39000000 INFO @ Wed, 15 Apr 2020 23:42:09: 34000000 INFO @ Wed, 15 Apr 2020 23:42:10: 34000000 INFO @ Wed, 15 Apr 2020 23:42:14: 40000000 INFO @ Wed, 15 Apr 2020 23:42:19: 35000000 INFO @ Wed, 15 Apr 2020 23:42:20: 35000000 INFO @ Wed, 15 Apr 2020 23:42:24: 41000000 INFO @ Wed, 15 Apr 2020 23:42:29: 36000000 INFO @ Wed, 15 Apr 2020 23:42:30: 36000000 INFO @ Wed, 15 Apr 2020 23:42:33: 42000000 INFO @ Wed, 15 Apr 2020 23:42:39: 37000000 INFO @ Wed, 15 Apr 2020 23:42:40: 37000000 INFO @ Wed, 15 Apr 2020 23:42:43: 43000000 INFO @ Wed, 15 Apr 2020 23:42:49: 38000000 INFO @ Wed, 15 Apr 2020 23:42:50: 38000000 INFO @ Wed, 15 Apr 2020 23:42:53: 44000000 INFO @ Wed, 15 Apr 2020 23:42:59: 39000000 INFO @ Wed, 15 Apr 2020 23:43:00: 39000000 INFO @ Wed, 15 Apr 2020 23:43:02: 45000000 INFO @ Wed, 15 Apr 2020 23:43:09: 40000000 INFO @ Wed, 15 Apr 2020 23:43:10: 40000000 INFO @ Wed, 15 Apr 2020 23:43:12: 46000000 INFO @ Wed, 15 Apr 2020 23:43:19: 41000000 INFO @ Wed, 15 Apr 2020 23:43:20: 41000000 INFO @ Wed, 15 Apr 2020 23:43:20: #1 tag size is determined as 150 bps INFO @ Wed, 15 Apr 2020 23:43:20: #1 tag size = 150 INFO @ Wed, 15 Apr 2020 23:43:20: #1 total tags in treatment: 17509829 INFO @ Wed, 15 Apr 2020 23:43:20: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:43:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:43:21: #1 tags after filtering in treatment: 15103778 INFO @ Wed, 15 Apr 2020 23:43:21: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 15 Apr 2020 23:43:21: #1 finished! INFO @ Wed, 15 Apr 2020 23:43:21: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:43:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:43:22: #2 number of paired peaks: 241 WARNING @ Wed, 15 Apr 2020 23:43:22: Fewer paired peaks (241) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 241 pairs to build model! INFO @ Wed, 15 Apr 2020 23:43:22: start model_add_line... INFO @ Wed, 15 Apr 2020 23:43:22: start X-correlation... INFO @ Wed, 15 Apr 2020 23:43:22: end of X-cor INFO @ Wed, 15 Apr 2020 23:43:22: #2 finished! INFO @ Wed, 15 Apr 2020 23:43:22: #2 predicted fragment length is 203 bps INFO @ Wed, 15 Apr 2020 23:43:22: #2 alternative fragment length(s) may be 4,199,203 bps INFO @ Wed, 15 Apr 2020 23:43:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.05_model.r WARNING @ Wed, 15 Apr 2020 23:43:22: #2 Since the d (203) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 23:43:22: #2 You may need to consider one of the other alternative d(s): 4,199,203 WARNING @ Wed, 15 Apr 2020 23:43:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 23:43:22: #3 Call peaks... INFO @ Wed, 15 Apr 2020 23:43:22: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 15 Apr 2020 23:43:29: 42000000 INFO @ Wed, 15 Apr 2020 23:43:29: 42000000 INFO @ Wed, 15 Apr 2020 23:43:38: 43000000 INFO @ Wed, 15 Apr 2020 23:43:39: 43000000 INFO @ Wed, 15 Apr 2020 23:43:47: 44000000 INFO @ Wed, 15 Apr 2020 23:43:48: 44000000 INFO @ Wed, 15 Apr 2020 23:43:51: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 23:43:57: 45000000 INFO @ Wed, 15 Apr 2020 23:43:57: 45000000 INFO @ Wed, 15 Apr 2020 23:44:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.05_peaks.xls INFO @ Wed, 15 Apr 2020 23:44:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.05_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 23:44:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.05_summits.bed INFO @ Wed, 15 Apr 2020 23:44:04: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (442 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 23:44:06: 46000000 INFO @ Wed, 15 Apr 2020 23:44:07: 46000000 INFO @ Wed, 15 Apr 2020 23:44:15: #1 tag size is determined as 150 bps INFO @ Wed, 15 Apr 2020 23:44:15: #1 tag size = 150 INFO @ Wed, 15 Apr 2020 23:44:15: #1 total tags in treatment: 17509829 INFO @ Wed, 15 Apr 2020 23:44:15: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:44:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:44:16: #1 tags after filtering in treatment: 15103778 INFO @ Wed, 15 Apr 2020 23:44:16: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 15 Apr 2020 23:44:16: #1 finished! INFO @ Wed, 15 Apr 2020 23:44:16: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:44:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:44:16: #1 tag size is determined as 150 bps INFO @ Wed, 15 Apr 2020 23:44:16: #1 tag size = 150 INFO @ Wed, 15 Apr 2020 23:44:16: #1 total tags in treatment: 17509829 INFO @ Wed, 15 Apr 2020 23:44:16: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:44:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:44:16: #1 tags after filtering in treatment: 15103778 INFO @ Wed, 15 Apr 2020 23:44:16: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 15 Apr 2020 23:44:16: #1 finished! INFO @ Wed, 15 Apr 2020 23:44:16: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:44:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:44:17: #2 number of paired peaks: 241 WARNING @ Wed, 15 Apr 2020 23:44:17: Fewer paired peaks (241) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 241 pairs to build model! INFO @ Wed, 15 Apr 2020 23:44:17: start model_add_line... INFO @ Wed, 15 Apr 2020 23:44:17: start X-correlation... INFO @ Wed, 15 Apr 2020 23:44:17: end of X-cor INFO @ Wed, 15 Apr 2020 23:44:17: #2 finished! INFO @ Wed, 15 Apr 2020 23:44:17: #2 predicted fragment length is 203 bps INFO @ Wed, 15 Apr 2020 23:44:17: #2 alternative fragment length(s) may be 4,199,203 bps INFO @ Wed, 15 Apr 2020 23:44:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.10_model.r WARNING @ Wed, 15 Apr 2020 23:44:17: #2 Since the d (203) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 23:44:17: #2 You may need to consider one of the other alternative d(s): 4,199,203 WARNING @ Wed, 15 Apr 2020 23:44:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 23:44:17: #3 Call peaks... INFO @ Wed, 15 Apr 2020 23:44:17: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 15 Apr 2020 23:44:17: #2 number of paired peaks: 241 WARNING @ Wed, 15 Apr 2020 23:44:17: Fewer paired peaks (241) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 241 pairs to build model! INFO @ Wed, 15 Apr 2020 23:44:17: start model_add_line... INFO @ Wed, 15 Apr 2020 23:44:17: start X-correlation... INFO @ Wed, 15 Apr 2020 23:44:17: end of X-cor INFO @ Wed, 15 Apr 2020 23:44:17: #2 finished! INFO @ Wed, 15 Apr 2020 23:44:17: #2 predicted fragment length is 203 bps INFO @ Wed, 15 Apr 2020 23:44:17: #2 alternative fragment length(s) may be 4,199,203 bps INFO @ Wed, 15 Apr 2020 23:44:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.20_model.r WARNING @ Wed, 15 Apr 2020 23:44:17: #2 Since the d (203) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 23:44:17: #2 You may need to consider one of the other alternative d(s): 4,199,203 WARNING @ Wed, 15 Apr 2020 23:44:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 23:44:17: #3 Call peaks... INFO @ Wed, 15 Apr 2020 23:44:17: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 15 Apr 2020 23:44:46: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 23:44:46: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 23:44:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.10_peaks.xls INFO @ Wed, 15 Apr 2020 23:44:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.10_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 23:44:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.10_summits.bed INFO @ Wed, 15 Apr 2020 23:44:58: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (332 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 23:44:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.20_peaks.xls INFO @ Wed, 15 Apr 2020 23:44:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.20_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 23:44:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619568/SRX6619568.20_summits.bed INFO @ Wed, 15 Apr 2020 23:44:59: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (223 records, 4 fields): 1 millis CompletedMACS2peakCalling BigWig に変換しました。