Job ID = 5720187 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 41,642,076 reads read : 83,284,152 reads written : 83,284,152 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:07:31 41642076 reads; of these: 41642076 (100.00%) were paired; of these: 25442308 (61.10%) aligned concordantly 0 times 13340754 (32.04%) aligned concordantly exactly 1 time 2859014 (6.87%) aligned concordantly >1 times ---- 25442308 pairs aligned concordantly 0 times; of these: 13068881 (51.37%) aligned discordantly 1 time ---- 12373427 pairs aligned 0 times concordantly or discordantly; of these: 24746854 mates make up the pairs; of these: 21313639 (86.13%) aligned 0 times 1142311 (4.62%) aligned exactly 1 time 2290904 (9.26%) aligned >1 times 74.41% overall alignment rate Time searching: 01:07:31 Overall time: 01:07:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4266655 / 28689315 = 0.1487 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:06:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:06:34: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:06:34: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:06:41: 1000000 INFO @ Thu, 16 Apr 2020 00:06:48: 2000000 INFO @ Thu, 16 Apr 2020 00:06:56: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:07:03: 4000000 INFO @ Thu, 16 Apr 2020 00:07:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:07:05: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:07:05: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:07:10: 5000000 INFO @ Thu, 16 Apr 2020 00:07:13: 1000000 INFO @ Thu, 16 Apr 2020 00:07:18: 6000000 INFO @ Thu, 16 Apr 2020 00:07:21: 2000000 INFO @ Thu, 16 Apr 2020 00:07:26: 7000000 INFO @ Thu, 16 Apr 2020 00:07:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:07:34: 8000000 INFO @ Thu, 16 Apr 2020 00:07:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:07:34: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:07:34: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:07:37: 4000000 INFO @ Thu, 16 Apr 2020 00:07:42: 9000000 INFO @ Thu, 16 Apr 2020 00:07:43: 1000000 INFO @ Thu, 16 Apr 2020 00:07:45: 5000000 INFO @ Thu, 16 Apr 2020 00:07:51: 10000000 INFO @ Thu, 16 Apr 2020 00:07:51: 2000000 INFO @ Thu, 16 Apr 2020 00:07:53: 6000000 INFO @ Thu, 16 Apr 2020 00:07:59: 11000000 INFO @ Thu, 16 Apr 2020 00:07:59: 3000000 INFO @ Thu, 16 Apr 2020 00:08:01: 7000000 INFO @ Thu, 16 Apr 2020 00:08:07: 12000000 INFO @ Thu, 16 Apr 2020 00:08:07: 4000000 INFO @ Thu, 16 Apr 2020 00:08:09: 8000000 INFO @ Thu, 16 Apr 2020 00:08:16: 13000000 INFO @ Thu, 16 Apr 2020 00:08:16: 5000000 INFO @ Thu, 16 Apr 2020 00:08:17: 9000000 INFO @ Thu, 16 Apr 2020 00:08:24: 14000000 INFO @ Thu, 16 Apr 2020 00:08:24: 6000000 INFO @ Thu, 16 Apr 2020 00:08:25: 10000000 INFO @ Thu, 16 Apr 2020 00:08:32: 15000000 INFO @ Thu, 16 Apr 2020 00:08:33: 7000000 INFO @ Thu, 16 Apr 2020 00:08:33: 11000000 INFO @ Thu, 16 Apr 2020 00:08:41: 16000000 INFO @ Thu, 16 Apr 2020 00:08:41: 12000000 INFO @ Thu, 16 Apr 2020 00:08:41: 8000000 INFO @ Thu, 16 Apr 2020 00:08:49: 13000000 INFO @ Thu, 16 Apr 2020 00:08:49: 17000000 INFO @ Thu, 16 Apr 2020 00:08:49: 9000000 INFO @ Thu, 16 Apr 2020 00:08:57: 14000000 INFO @ Thu, 16 Apr 2020 00:08:57: 18000000 INFO @ Thu, 16 Apr 2020 00:08:57: 10000000 INFO @ Thu, 16 Apr 2020 00:09:05: 15000000 INFO @ Thu, 16 Apr 2020 00:09:06: 19000000 INFO @ Thu, 16 Apr 2020 00:09:06: 11000000 INFO @ Thu, 16 Apr 2020 00:09:13: 16000000 INFO @ Thu, 16 Apr 2020 00:09:14: 20000000 INFO @ Thu, 16 Apr 2020 00:09:14: 12000000 INFO @ Thu, 16 Apr 2020 00:09:21: 17000000 INFO @ Thu, 16 Apr 2020 00:09:22: 21000000 INFO @ Thu, 16 Apr 2020 00:09:22: 13000000 INFO @ Thu, 16 Apr 2020 00:09:29: 18000000 INFO @ Thu, 16 Apr 2020 00:09:30: 22000000 INFO @ Thu, 16 Apr 2020 00:09:30: 14000000 INFO @ Thu, 16 Apr 2020 00:09:37: 19000000 INFO @ Thu, 16 Apr 2020 00:09:39: 23000000 INFO @ Thu, 16 Apr 2020 00:09:39: 15000000 INFO @ Thu, 16 Apr 2020 00:09:46: 20000000 INFO @ Thu, 16 Apr 2020 00:09:47: 24000000 INFO @ Thu, 16 Apr 2020 00:09:47: 16000000 INFO @ Thu, 16 Apr 2020 00:09:54: 21000000 INFO @ Thu, 16 Apr 2020 00:09:55: 25000000 INFO @ Thu, 16 Apr 2020 00:09:55: 17000000 INFO @ Thu, 16 Apr 2020 00:10:02: 22000000 INFO @ Thu, 16 Apr 2020 00:10:04: 26000000 INFO @ Thu, 16 Apr 2020 00:10:04: 18000000 INFO @ Thu, 16 Apr 2020 00:10:10: 23000000 INFO @ Thu, 16 Apr 2020 00:10:12: 27000000 INFO @ Thu, 16 Apr 2020 00:10:12: 19000000 INFO @ Thu, 16 Apr 2020 00:10:18: 24000000 INFO @ Thu, 16 Apr 2020 00:10:20: 28000000 INFO @ Thu, 16 Apr 2020 00:10:20: 20000000 INFO @ Thu, 16 Apr 2020 00:10:26: 25000000 INFO @ Thu, 16 Apr 2020 00:10:29: 29000000 INFO @ Thu, 16 Apr 2020 00:10:29: 21000000 INFO @ Thu, 16 Apr 2020 00:10:34: 26000000 INFO @ Thu, 16 Apr 2020 00:10:37: 30000000 INFO @ Thu, 16 Apr 2020 00:10:37: 22000000 INFO @ Thu, 16 Apr 2020 00:10:42: 27000000 INFO @ Thu, 16 Apr 2020 00:10:45: 31000000 INFO @ Thu, 16 Apr 2020 00:10:45: 23000000 INFO @ Thu, 16 Apr 2020 00:10:50: 28000000 INFO @ Thu, 16 Apr 2020 00:10:53: 32000000 INFO @ Thu, 16 Apr 2020 00:10:53: 24000000 INFO @ Thu, 16 Apr 2020 00:10:58: 29000000 INFO @ Thu, 16 Apr 2020 00:11:01: 25000000 INFO @ Thu, 16 Apr 2020 00:11:01: 33000000 INFO @ Thu, 16 Apr 2020 00:11:06: 30000000 INFO @ Thu, 16 Apr 2020 00:11:09: 26000000 INFO @ Thu, 16 Apr 2020 00:11:09: 34000000 INFO @ Thu, 16 Apr 2020 00:11:15: 31000000 INFO @ Thu, 16 Apr 2020 00:11:17: 27000000 INFO @ Thu, 16 Apr 2020 00:11:18: 35000000 INFO @ Thu, 16 Apr 2020 00:11:23: 32000000 INFO @ Thu, 16 Apr 2020 00:11:26: 36000000 INFO @ Thu, 16 Apr 2020 00:11:26: 28000000 INFO @ Thu, 16 Apr 2020 00:11:31: 33000000 INFO @ Thu, 16 Apr 2020 00:11:33: 37000000 INFO @ Thu, 16 Apr 2020 00:11:34: 29000000 INFO @ Thu, 16 Apr 2020 00:11:39: 34000000 INFO @ Thu, 16 Apr 2020 00:11:41: 38000000 INFO @ Thu, 16 Apr 2020 00:11:42: 30000000 INFO @ Thu, 16 Apr 2020 00:11:47: 35000000 INFO @ Thu, 16 Apr 2020 00:11:49: 39000000 INFO @ Thu, 16 Apr 2020 00:11:50: 31000000 INFO @ Thu, 16 Apr 2020 00:11:55: 36000000 INFO @ Thu, 16 Apr 2020 00:11:57: 40000000 INFO @ Thu, 16 Apr 2020 00:11:58: 32000000 INFO @ Thu, 16 Apr 2020 00:12:03: 37000000 INFO @ Thu, 16 Apr 2020 00:12:05: 41000000 INFO @ Thu, 16 Apr 2020 00:12:06: 33000000 INFO @ Thu, 16 Apr 2020 00:12:11: 38000000 INFO @ Thu, 16 Apr 2020 00:12:13: 42000000 INFO @ Thu, 16 Apr 2020 00:12:15: 34000000 INFO @ Thu, 16 Apr 2020 00:12:19: 39000000 INFO @ Thu, 16 Apr 2020 00:12:21: 43000000 INFO @ Thu, 16 Apr 2020 00:12:23: 35000000 INFO @ Thu, 16 Apr 2020 00:12:26: 40000000 INFO @ Thu, 16 Apr 2020 00:12:29: 44000000 INFO @ Thu, 16 Apr 2020 00:12:31: 36000000 INFO @ Thu, 16 Apr 2020 00:12:34: 41000000 INFO @ Thu, 16 Apr 2020 00:12:36: 45000000 INFO @ Thu, 16 Apr 2020 00:12:39: 37000000 INFO @ Thu, 16 Apr 2020 00:12:42: 42000000 INFO @ Thu, 16 Apr 2020 00:12:44: 46000000 INFO @ Thu, 16 Apr 2020 00:12:47: 38000000 INFO @ Thu, 16 Apr 2020 00:12:50: 43000000 INFO @ Thu, 16 Apr 2020 00:12:52: 47000000 INFO @ Thu, 16 Apr 2020 00:12:55: 39000000 INFO @ Thu, 16 Apr 2020 00:12:58: 44000000 INFO @ Thu, 16 Apr 2020 00:13:00: 48000000 INFO @ Thu, 16 Apr 2020 00:13:03: 40000000 INFO @ Thu, 16 Apr 2020 00:13:06: 45000000 INFO @ Thu, 16 Apr 2020 00:13:08: 49000000 INFO @ Thu, 16 Apr 2020 00:13:11: 41000000 INFO @ Thu, 16 Apr 2020 00:13:14: 46000000 INFO @ Thu, 16 Apr 2020 00:13:16: 50000000 INFO @ Thu, 16 Apr 2020 00:13:19: 42000000 INFO @ Thu, 16 Apr 2020 00:13:22: 47000000 INFO @ Thu, 16 Apr 2020 00:13:24: 51000000 INFO @ Thu, 16 Apr 2020 00:13:27: 43000000 INFO @ Thu, 16 Apr 2020 00:13:29: 48000000 INFO @ Thu, 16 Apr 2020 00:13:31: 52000000 INFO @ Thu, 16 Apr 2020 00:13:35: 44000000 INFO @ Thu, 16 Apr 2020 00:13:37: 49000000 INFO @ Thu, 16 Apr 2020 00:13:39: 53000000 INFO @ Thu, 16 Apr 2020 00:13:43: 45000000 INFO @ Thu, 16 Apr 2020 00:13:43: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:13:43: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:13:43: #1 total tags in treatment: 13579575 INFO @ Thu, 16 Apr 2020 00:13:43: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:13:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:13:43: #1 tags after filtering in treatment: 11370108 INFO @ Thu, 16 Apr 2020 00:13:43: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:13:43: #1 finished! INFO @ Thu, 16 Apr 2020 00:13:43: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:13:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:13:44: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:13:44: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:13:44: start model_add_line... INFO @ Thu, 16 Apr 2020 00:13:44: start X-correlation... INFO @ Thu, 16 Apr 2020 00:13:44: end of X-cor INFO @ Thu, 16 Apr 2020 00:13:44: #2 finished! INFO @ Thu, 16 Apr 2020 00:13:44: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:13:44: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:13:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.05_model.r WARNING @ Thu, 16 Apr 2020 00:13:44: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:13:44: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:13:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:13:44: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:13:44: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:13:45: 50000000 INFO @ Thu, 16 Apr 2020 00:13:51: 46000000 INFO @ Thu, 16 Apr 2020 00:13:53: 51000000 INFO @ Thu, 16 Apr 2020 00:13:58: 47000000 INFO @ Thu, 16 Apr 2020 00:14:00: 52000000 INFO @ Thu, 16 Apr 2020 00:14:06: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:14:06: 48000000 INFO @ Thu, 16 Apr 2020 00:14:08: 53000000 INFO @ Thu, 16 Apr 2020 00:14:12: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:14:12: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:14:12: #1 total tags in treatment: 13579575 INFO @ Thu, 16 Apr 2020 00:14:12: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:14:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:14:12: #1 tags after filtering in treatment: 11370108 INFO @ Thu, 16 Apr 2020 00:14:12: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:14:12: #1 finished! INFO @ Thu, 16 Apr 2020 00:14:12: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:14:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:14:13: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:14:13: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:14:13: start model_add_line... INFO @ Thu, 16 Apr 2020 00:14:13: start X-correlation... INFO @ Thu, 16 Apr 2020 00:14:13: end of X-cor INFO @ Thu, 16 Apr 2020 00:14:13: #2 finished! INFO @ Thu, 16 Apr 2020 00:14:13: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:14:13: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:14:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.10_model.r WARNING @ Thu, 16 Apr 2020 00:14:13: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:14:13: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:14:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:14:13: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:14:13: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:14:14: 49000000 INFO @ Thu, 16 Apr 2020 00:14:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:14:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:14:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.05_summits.bed INFO @ Thu, 16 Apr 2020 00:14:16: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (523 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:14:22: 50000000 INFO @ Thu, 16 Apr 2020 00:14:29: 51000000 INFO @ Thu, 16 Apr 2020 00:14:36: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:14:37: 52000000 INFO @ Thu, 16 Apr 2020 00:14:44: 53000000 INFO @ Thu, 16 Apr 2020 00:14:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:14:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:14:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.10_summits.bed INFO @ Thu, 16 Apr 2020 00:14:46: Done! INFO @ Thu, 16 Apr 2020 00:14:47: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:14:47: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:14:47: #1 total tags in treatment: 13579575 INFO @ Thu, 16 Apr 2020 00:14:47: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:14:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:14:47: #1 tags after filtering in treatment: 11370108 INFO @ Thu, 16 Apr 2020 00:14:47: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:14:47: #1 finished! INFO @ Thu, 16 Apr 2020 00:14:47: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:14:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:14:48: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:14:48: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:14:48: start model_add_line... INFO @ Thu, 16 Apr 2020 00:14:48: start X-correlation... INFO @ Thu, 16 Apr 2020 00:14:48: end of X-cor INFO @ Thu, 16 Apr 2020 00:14:48: #2 finished! INFO @ Thu, 16 Apr 2020 00:14:48: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:14:48: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:14:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.20_model.r WARNING @ Thu, 16 Apr 2020 00:14:48: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:14:48: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:14:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:14:48: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:14:48: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (357 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:15:10: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:15:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:15:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:15:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619557/SRX6619557.20_summits.bed INFO @ Thu, 16 Apr 2020 00:15:20: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (221 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。