Job ID = 5720181 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:19:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:21:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:26:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:32:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 31,867,768 reads read : 63,735,536 reads written : 63,735,536 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:24:26 31867768 reads; of these: 31867768 (100.00%) were paired; of these: 15034123 (47.18%) aligned concordantly 0 times 13811921 (43.34%) aligned concordantly exactly 1 time 3021724 (9.48%) aligned concordantly >1 times ---- 15034123 pairs aligned concordantly 0 times; of these: 7464300 (49.65%) aligned discordantly 1 time ---- 7569823 pairs aligned 0 times concordantly or discordantly; of these: 15139646 mates make up the pairs; of these: 12348585 (81.56%) aligned 0 times 1077258 (7.12%) aligned exactly 1 time 1713803 (11.32%) aligned >1 times 80.63% overall alignment rate Time searching: 01:24:26 Overall time: 01:24:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3140740 / 23943225 = 0.1312 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:15:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:15:41: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:15:41: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:15:52: 1000000 INFO @ Thu, 16 Apr 2020 00:16:03: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:16:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:16:12: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:16:12: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:16:14: 3000000 INFO @ Thu, 16 Apr 2020 00:16:24: 4000000 INFO @ Thu, 16 Apr 2020 00:16:25: 1000000 INFO @ Thu, 16 Apr 2020 00:16:34: 5000000 INFO @ Thu, 16 Apr 2020 00:16:39: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:16:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:16:42: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:16:42: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:16:43: 6000000 INFO @ Thu, 16 Apr 2020 00:16:52: 3000000 INFO @ Thu, 16 Apr 2020 00:16:52: 7000000 INFO @ Thu, 16 Apr 2020 00:16:55: 1000000 INFO @ Thu, 16 Apr 2020 00:17:01: 8000000 INFO @ Thu, 16 Apr 2020 00:17:05: 4000000 INFO @ Thu, 16 Apr 2020 00:17:08: 2000000 INFO @ Thu, 16 Apr 2020 00:17:11: 9000000 INFO @ Thu, 16 Apr 2020 00:17:17: 5000000 INFO @ Thu, 16 Apr 2020 00:17:19: 3000000 INFO @ Thu, 16 Apr 2020 00:17:20: 10000000 INFO @ Thu, 16 Apr 2020 00:17:29: 6000000 INFO @ Thu, 16 Apr 2020 00:17:29: 11000000 INFO @ Thu, 16 Apr 2020 00:17:32: 4000000 INFO @ Thu, 16 Apr 2020 00:17:39: 12000000 INFO @ Thu, 16 Apr 2020 00:17:40: 7000000 INFO @ Thu, 16 Apr 2020 00:17:43: 5000000 INFO @ Thu, 16 Apr 2020 00:17:48: 13000000 INFO @ Thu, 16 Apr 2020 00:17:50: 8000000 INFO @ Thu, 16 Apr 2020 00:17:53: 6000000 INFO @ Thu, 16 Apr 2020 00:17:58: 14000000 INFO @ Thu, 16 Apr 2020 00:18:00: 9000000 INFO @ Thu, 16 Apr 2020 00:18:03: 7000000 INFO @ Thu, 16 Apr 2020 00:18:06: 15000000 INFO @ Thu, 16 Apr 2020 00:18:10: 10000000 INFO @ Thu, 16 Apr 2020 00:18:13: 8000000 INFO @ Thu, 16 Apr 2020 00:18:16: 16000000 INFO @ Thu, 16 Apr 2020 00:18:21: 11000000 INFO @ Thu, 16 Apr 2020 00:18:23: 9000000 INFO @ Thu, 16 Apr 2020 00:18:25: 17000000 INFO @ Thu, 16 Apr 2020 00:18:31: 12000000 INFO @ Thu, 16 Apr 2020 00:18:33: 10000000 INFO @ Thu, 16 Apr 2020 00:18:34: 18000000 INFO @ Thu, 16 Apr 2020 00:18:41: 13000000 INFO @ Thu, 16 Apr 2020 00:18:43: 11000000 INFO @ Thu, 16 Apr 2020 00:18:49: 19000000 INFO @ Thu, 16 Apr 2020 00:18:50: 14000000 INFO @ Thu, 16 Apr 2020 00:18:53: 12000000 INFO @ Thu, 16 Apr 2020 00:18:58: 20000000 INFO @ Thu, 16 Apr 2020 00:19:00: 15000000 INFO @ Thu, 16 Apr 2020 00:19:04: 13000000 INFO @ Thu, 16 Apr 2020 00:19:07: 21000000 INFO @ Thu, 16 Apr 2020 00:19:11: 16000000 INFO @ Thu, 16 Apr 2020 00:19:14: 14000000 INFO @ Thu, 16 Apr 2020 00:19:16: 22000000 INFO @ Thu, 16 Apr 2020 00:19:21: 17000000 INFO @ Thu, 16 Apr 2020 00:19:24: 15000000 INFO @ Thu, 16 Apr 2020 00:19:25: 23000000 INFO @ Thu, 16 Apr 2020 00:19:31: 18000000 INFO @ Thu, 16 Apr 2020 00:19:33: 16000000 INFO @ Thu, 16 Apr 2020 00:19:34: 24000000 INFO @ Thu, 16 Apr 2020 00:19:41: 19000000 INFO @ Thu, 16 Apr 2020 00:19:43: 25000000 INFO @ Thu, 16 Apr 2020 00:19:44: 17000000 INFO @ Thu, 16 Apr 2020 00:19:51: 20000000 INFO @ Thu, 16 Apr 2020 00:19:52: 26000000 INFO @ Thu, 16 Apr 2020 00:19:55: 18000000 INFO @ Thu, 16 Apr 2020 00:20:01: 27000000 INFO @ Thu, 16 Apr 2020 00:20:02: 21000000 INFO @ Thu, 16 Apr 2020 00:20:05: 19000000 INFO @ Thu, 16 Apr 2020 00:20:10: 28000000 INFO @ Thu, 16 Apr 2020 00:20:12: 22000000 INFO @ Thu, 16 Apr 2020 00:20:15: 20000000 INFO @ Thu, 16 Apr 2020 00:20:19: 29000000 INFO @ Thu, 16 Apr 2020 00:20:21: 23000000 INFO @ Thu, 16 Apr 2020 00:20:25: 21000000 INFO @ Thu, 16 Apr 2020 00:20:29: 30000000 INFO @ Thu, 16 Apr 2020 00:20:31: 24000000 INFO @ Thu, 16 Apr 2020 00:20:34: 22000000 INFO @ Thu, 16 Apr 2020 00:20:38: 31000000 INFO @ Thu, 16 Apr 2020 00:20:41: 25000000 INFO @ Thu, 16 Apr 2020 00:20:44: 23000000 INFO @ Thu, 16 Apr 2020 00:20:47: 32000000 INFO @ Thu, 16 Apr 2020 00:20:51: 26000000 INFO @ Thu, 16 Apr 2020 00:20:54: 24000000 INFO @ Thu, 16 Apr 2020 00:20:57: 33000000 INFO @ Thu, 16 Apr 2020 00:21:01: 27000000 INFO @ Thu, 16 Apr 2020 00:21:04: 25000000 INFO @ Thu, 16 Apr 2020 00:21:06: 34000000 INFO @ Thu, 16 Apr 2020 00:21:11: 28000000 INFO @ Thu, 16 Apr 2020 00:21:14: 26000000 INFO @ Thu, 16 Apr 2020 00:21:15: 35000000 INFO @ Thu, 16 Apr 2020 00:21:22: 29000000 INFO @ Thu, 16 Apr 2020 00:21:25: 36000000 INFO @ Thu, 16 Apr 2020 00:21:25: 27000000 INFO @ Thu, 16 Apr 2020 00:21:33: 30000000 INFO @ Thu, 16 Apr 2020 00:21:34: 37000000 INFO @ Thu, 16 Apr 2020 00:21:36: 28000000 INFO @ Thu, 16 Apr 2020 00:21:42: 31000000 INFO @ Thu, 16 Apr 2020 00:21:43: 38000000 INFO @ Thu, 16 Apr 2020 00:21:45: 29000000 INFO @ Thu, 16 Apr 2020 00:21:52: 32000000 INFO @ Thu, 16 Apr 2020 00:21:52: 39000000 INFO @ Thu, 16 Apr 2020 00:21:55: 30000000 INFO @ Thu, 16 Apr 2020 00:22:02: 33000000 INFO @ Thu, 16 Apr 2020 00:22:05: 40000000 INFO @ Thu, 16 Apr 2020 00:22:06: 31000000 INFO @ Thu, 16 Apr 2020 00:22:12: 34000000 INFO @ Thu, 16 Apr 2020 00:22:16: 32000000 INFO @ Thu, 16 Apr 2020 00:22:17: 41000000 INFO @ Thu, 16 Apr 2020 00:22:22: 35000000 INFO @ Thu, 16 Apr 2020 00:22:26: 42000000 INFO @ Thu, 16 Apr 2020 00:22:27: 33000000 INFO @ Thu, 16 Apr 2020 00:22:34: 36000000 INFO @ Thu, 16 Apr 2020 00:22:35: 43000000 INFO @ Thu, 16 Apr 2020 00:22:37: 34000000 INFO @ Thu, 16 Apr 2020 00:22:44: 37000000 INFO @ Thu, 16 Apr 2020 00:22:44: 44000000 INFO @ Thu, 16 Apr 2020 00:22:47: 35000000 INFO @ Thu, 16 Apr 2020 00:22:53: 45000000 INFO @ Thu, 16 Apr 2020 00:22:54: 38000000 INFO @ Thu, 16 Apr 2020 00:22:54: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:22:54: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:22:54: #1 total tags in treatment: 14482910 INFO @ Thu, 16 Apr 2020 00:22:54: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:22:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:22:54: #1 tags after filtering in treatment: 12188486 INFO @ Thu, 16 Apr 2020 00:22:54: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:22:54: #1 finished! INFO @ Thu, 16 Apr 2020 00:22:54: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:22:54: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:22:55: #2 number of paired peaks: 296 WARNING @ Thu, 16 Apr 2020 00:22:55: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Thu, 16 Apr 2020 00:22:55: start model_add_line... INFO @ Thu, 16 Apr 2020 00:22:55: start X-correlation... INFO @ Thu, 16 Apr 2020 00:22:55: end of X-cor INFO @ Thu, 16 Apr 2020 00:22:55: #2 finished! INFO @ Thu, 16 Apr 2020 00:22:55: #2 predicted fragment length is 207 bps INFO @ Thu, 16 Apr 2020 00:22:55: #2 alternative fragment length(s) may be 207 bps INFO @ Thu, 16 Apr 2020 00:22:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.05_model.r WARNING @ Thu, 16 Apr 2020 00:22:55: #2 Since the d (207) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:22:55: #2 You may need to consider one of the other alternative d(s): 207 WARNING @ Thu, 16 Apr 2020 00:22:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:22:55: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:22:55: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:22:57: 36000000 INFO @ Thu, 16 Apr 2020 00:23:03: 39000000 INFO @ Thu, 16 Apr 2020 00:23:07: 37000000 INFO @ Thu, 16 Apr 2020 00:23:13: 40000000 INFO @ Thu, 16 Apr 2020 00:23:18: 38000000 INFO @ Thu, 16 Apr 2020 00:23:20: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:23:23: 41000000 INFO @ Thu, 16 Apr 2020 00:23:27: 39000000 INFO @ Thu, 16 Apr 2020 00:23:32: 42000000 INFO @ Thu, 16 Apr 2020 00:23:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:23:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:23:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.05_summits.bed INFO @ Thu, 16 Apr 2020 00:23:33: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (561 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:23:36: 40000000 INFO @ Thu, 16 Apr 2020 00:23:42: 43000000 INFO @ Thu, 16 Apr 2020 00:23:45: 41000000 INFO @ Thu, 16 Apr 2020 00:23:51: 44000000 INFO @ Thu, 16 Apr 2020 00:23:55: 42000000 INFO @ Thu, 16 Apr 2020 00:24:00: 45000000 INFO @ Thu, 16 Apr 2020 00:24:01: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:24:01: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:24:01: #1 total tags in treatment: 14482910 INFO @ Thu, 16 Apr 2020 00:24:01: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:24:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:24:02: #1 tags after filtering in treatment: 12188486 INFO @ Thu, 16 Apr 2020 00:24:02: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:24:02: #1 finished! INFO @ Thu, 16 Apr 2020 00:24:02: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:24:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:24:02: #2 number of paired peaks: 296 WARNING @ Thu, 16 Apr 2020 00:24:02: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Thu, 16 Apr 2020 00:24:02: start model_add_line... INFO @ Thu, 16 Apr 2020 00:24:03: start X-correlation... INFO @ Thu, 16 Apr 2020 00:24:03: end of X-cor INFO @ Thu, 16 Apr 2020 00:24:03: #2 finished! INFO @ Thu, 16 Apr 2020 00:24:03: #2 predicted fragment length is 207 bps INFO @ Thu, 16 Apr 2020 00:24:03: #2 alternative fragment length(s) may be 207 bps INFO @ Thu, 16 Apr 2020 00:24:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.10_model.r WARNING @ Thu, 16 Apr 2020 00:24:03: #2 Since the d (207) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:24:03: #2 You may need to consider one of the other alternative d(s): 207 WARNING @ Thu, 16 Apr 2020 00:24:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:24:03: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:24:03: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:24:04: 43000000 INFO @ Thu, 16 Apr 2020 00:24:12: 44000000 INFO @ Thu, 16 Apr 2020 00:24:21: 45000000 INFO @ Thu, 16 Apr 2020 00:24:22: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:24:22: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:24:22: #1 total tags in treatment: 14482910 INFO @ Thu, 16 Apr 2020 00:24:22: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:24:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:24:22: #1 tags after filtering in treatment: 12188486 INFO @ Thu, 16 Apr 2020 00:24:22: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:24:22: #1 finished! INFO @ Thu, 16 Apr 2020 00:24:22: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:24:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:24:23: #2 number of paired peaks: 296 WARNING @ Thu, 16 Apr 2020 00:24:23: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Thu, 16 Apr 2020 00:24:23: start model_add_line... INFO @ Thu, 16 Apr 2020 00:24:23: start X-correlation... INFO @ Thu, 16 Apr 2020 00:24:23: end of X-cor INFO @ Thu, 16 Apr 2020 00:24:23: #2 finished! INFO @ Thu, 16 Apr 2020 00:24:23: #2 predicted fragment length is 207 bps INFO @ Thu, 16 Apr 2020 00:24:23: #2 alternative fragment length(s) may be 207 bps INFO @ Thu, 16 Apr 2020 00:24:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.20_model.r WARNING @ Thu, 16 Apr 2020 00:24:23: #2 Since the d (207) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:24:23: #2 You may need to consider one of the other alternative d(s): 207 WARNING @ Thu, 16 Apr 2020 00:24:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:24:23: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:24:23: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:24:27: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:24:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:24:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:24:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.10_summits.bed INFO @ Thu, 16 Apr 2020 00:24:40: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (375 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:24:47: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:25:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:25:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:25:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619552/SRX6619552.20_summits.bed INFO @ Thu, 16 Apr 2020 00:25:00: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (236 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。