Job ID = 5720177 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:52:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:55:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:58:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:07:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:08:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 31,790,244 reads read : 63,580,488 reads written : 63,580,488 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:33:48 31790244 reads; of these: 31790244 (100.00%) were paired; of these: 11416624 (35.91%) aligned concordantly 0 times 15815974 (49.75%) aligned concordantly exactly 1 time 4557646 (14.34%) aligned concordantly >1 times ---- 11416624 pairs aligned concordantly 0 times; of these: 6587314 (57.70%) aligned discordantly 1 time ---- 4829310 pairs aligned 0 times concordantly or discordantly; of these: 9658620 mates make up the pairs; of these: 6083233 (62.98%) aligned 0 times 1476448 (15.29%) aligned exactly 1 time 2098939 (21.73%) aligned >1 times 90.43% overall alignment rate Time searching: 01:33:48 Overall time: 01:33:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4558421 / 26363827 = 0.1729 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:25:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:25:26: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:25:26: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:25:36: 1000000 INFO @ Thu, 16 Apr 2020 00:25:46: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:25:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:25:56: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:25:56: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:25:56: 3000000 INFO @ Thu, 16 Apr 2020 00:26:06: 4000000 INFO @ Thu, 16 Apr 2020 00:26:07: 1000000 INFO @ Thu, 16 Apr 2020 00:26:17: 5000000 INFO @ Thu, 16 Apr 2020 00:26:18: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:26:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:26:26: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:26:26: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:26:29: 6000000 INFO @ Thu, 16 Apr 2020 00:26:30: 3000000 INFO @ Thu, 16 Apr 2020 00:26:36: 1000000 INFO @ Thu, 16 Apr 2020 00:26:40: 7000000 INFO @ Thu, 16 Apr 2020 00:26:42: 4000000 INFO @ Thu, 16 Apr 2020 00:26:45: 2000000 INFO @ Thu, 16 Apr 2020 00:26:51: 8000000 INFO @ Thu, 16 Apr 2020 00:26:54: 5000000 INFO @ Thu, 16 Apr 2020 00:26:55: 3000000 INFO @ Thu, 16 Apr 2020 00:27:02: 9000000 INFO @ Thu, 16 Apr 2020 00:27:05: 4000000 INFO @ Thu, 16 Apr 2020 00:27:05: 6000000 INFO @ Thu, 16 Apr 2020 00:27:14: 10000000 INFO @ Thu, 16 Apr 2020 00:27:15: 5000000 INFO @ Thu, 16 Apr 2020 00:27:17: 7000000 INFO @ Thu, 16 Apr 2020 00:27:24: 6000000 INFO @ Thu, 16 Apr 2020 00:27:25: 11000000 INFO @ Thu, 16 Apr 2020 00:27:28: 8000000 INFO @ Thu, 16 Apr 2020 00:27:34: 7000000 INFO @ Thu, 16 Apr 2020 00:27:36: 12000000 INFO @ Thu, 16 Apr 2020 00:27:40: 9000000 INFO @ Thu, 16 Apr 2020 00:27:44: 8000000 INFO @ Thu, 16 Apr 2020 00:27:47: 13000000 INFO @ Thu, 16 Apr 2020 00:27:51: 10000000 INFO @ Thu, 16 Apr 2020 00:27:55: 9000000 INFO @ Thu, 16 Apr 2020 00:27:59: 14000000 INFO @ Thu, 16 Apr 2020 00:28:03: 11000000 INFO @ Thu, 16 Apr 2020 00:28:05: 10000000 INFO @ Thu, 16 Apr 2020 00:28:10: 15000000 INFO @ Thu, 16 Apr 2020 00:28:14: 12000000 INFO @ Thu, 16 Apr 2020 00:28:15: 11000000 INFO @ Thu, 16 Apr 2020 00:28:21: 16000000 INFO @ Thu, 16 Apr 2020 00:28:24: 12000000 INFO @ Thu, 16 Apr 2020 00:28:26: 13000000 INFO @ Thu, 16 Apr 2020 00:28:33: 17000000 INFO @ Thu, 16 Apr 2020 00:28:34: 13000000 INFO @ Thu, 16 Apr 2020 00:28:37: 14000000 INFO @ Thu, 16 Apr 2020 00:28:43: 14000000 INFO @ Thu, 16 Apr 2020 00:28:44: 18000000 INFO @ Thu, 16 Apr 2020 00:28:50: 15000000 INFO @ Thu, 16 Apr 2020 00:28:54: 15000000 INFO @ Thu, 16 Apr 2020 00:28:57: 19000000 INFO @ Thu, 16 Apr 2020 00:29:01: 16000000 INFO @ Thu, 16 Apr 2020 00:29:04: 16000000 INFO @ Thu, 16 Apr 2020 00:29:07: 20000000 INFO @ Thu, 16 Apr 2020 00:29:13: 17000000 INFO @ Thu, 16 Apr 2020 00:29:15: 17000000 INFO @ Thu, 16 Apr 2020 00:29:19: 21000000 INFO @ Thu, 16 Apr 2020 00:29:25: 18000000 INFO @ Thu, 16 Apr 2020 00:29:25: 18000000 INFO @ Thu, 16 Apr 2020 00:29:31: 22000000 INFO @ Thu, 16 Apr 2020 00:29:34: 19000000 INFO @ Thu, 16 Apr 2020 00:29:37: 19000000 INFO @ Thu, 16 Apr 2020 00:29:43: 23000000 INFO @ Thu, 16 Apr 2020 00:29:43: 20000000 INFO @ Thu, 16 Apr 2020 00:29:49: 20000000 INFO @ Thu, 16 Apr 2020 00:29:53: 21000000 INFO @ Thu, 16 Apr 2020 00:29:55: 24000000 INFO @ Thu, 16 Apr 2020 00:30:01: 21000000 INFO @ Thu, 16 Apr 2020 00:30:02: 22000000 INFO @ Thu, 16 Apr 2020 00:30:06: 25000000 INFO @ Thu, 16 Apr 2020 00:30:11: 23000000 INFO @ Thu, 16 Apr 2020 00:30:12: 22000000 INFO @ Thu, 16 Apr 2020 00:30:18: 26000000 INFO @ Thu, 16 Apr 2020 00:30:21: 24000000 INFO @ Thu, 16 Apr 2020 00:30:25: 23000000 INFO @ Thu, 16 Apr 2020 00:30:29: 27000000 INFO @ Thu, 16 Apr 2020 00:30:30: 25000000 INFO @ Thu, 16 Apr 2020 00:30:37: 24000000 INFO @ Thu, 16 Apr 2020 00:30:39: 26000000 INFO @ Thu, 16 Apr 2020 00:30:41: 28000000 INFO @ Thu, 16 Apr 2020 00:30:48: 27000000 INFO @ Thu, 16 Apr 2020 00:30:49: 25000000 INFO @ Thu, 16 Apr 2020 00:30:53: 29000000 INFO @ Thu, 16 Apr 2020 00:30:57: 28000000 INFO @ Thu, 16 Apr 2020 00:31:01: 26000000 INFO @ Thu, 16 Apr 2020 00:31:05: 30000000 INFO @ Thu, 16 Apr 2020 00:31:07: 29000000 INFO @ Thu, 16 Apr 2020 00:31:13: 27000000 INFO @ Thu, 16 Apr 2020 00:31:16: 30000000 INFO @ Thu, 16 Apr 2020 00:31:16: 31000000 INFO @ Thu, 16 Apr 2020 00:31:24: 28000000 INFO @ Thu, 16 Apr 2020 00:31:26: 31000000 INFO @ Thu, 16 Apr 2020 00:31:28: 32000000 INFO @ Thu, 16 Apr 2020 00:31:35: 32000000 INFO @ Thu, 16 Apr 2020 00:31:36: 29000000 INFO @ Thu, 16 Apr 2020 00:31:40: 33000000 INFO @ Thu, 16 Apr 2020 00:31:44: 33000000 INFO @ Thu, 16 Apr 2020 00:31:49: 30000000 INFO @ Thu, 16 Apr 2020 00:31:52: 34000000 INFO @ Thu, 16 Apr 2020 00:31:54: 34000000 INFO @ Thu, 16 Apr 2020 00:32:01: 31000000 INFO @ Thu, 16 Apr 2020 00:32:03: 35000000 INFO @ Thu, 16 Apr 2020 00:32:04: 35000000 INFO @ Thu, 16 Apr 2020 00:32:12: 36000000 INFO @ Thu, 16 Apr 2020 00:32:14: 32000000 INFO @ Thu, 16 Apr 2020 00:32:16: 36000000 INFO @ Thu, 16 Apr 2020 00:32:22: 37000000 INFO @ Thu, 16 Apr 2020 00:32:26: 33000000 INFO @ Thu, 16 Apr 2020 00:32:29: 37000000 INFO @ Thu, 16 Apr 2020 00:32:31: 38000000 INFO @ Thu, 16 Apr 2020 00:32:38: 34000000 INFO @ Thu, 16 Apr 2020 00:32:41: 38000000 INFO @ Thu, 16 Apr 2020 00:32:42: 39000000 INFO @ Thu, 16 Apr 2020 00:32:50: 35000000 INFO @ Thu, 16 Apr 2020 00:32:52: 40000000 INFO @ Thu, 16 Apr 2020 00:32:52: 39000000 INFO @ Thu, 16 Apr 2020 00:33:02: 41000000 INFO @ Thu, 16 Apr 2020 00:33:02: 36000000 INFO @ Thu, 16 Apr 2020 00:33:05: 40000000 INFO @ Thu, 16 Apr 2020 00:33:11: 42000000 INFO @ Thu, 16 Apr 2020 00:33:14: 37000000 INFO @ Thu, 16 Apr 2020 00:33:16: 41000000 INFO @ Thu, 16 Apr 2020 00:33:20: 43000000 INFO @ Thu, 16 Apr 2020 00:33:26: 38000000 INFO @ Thu, 16 Apr 2020 00:33:28: 42000000 INFO @ Thu, 16 Apr 2020 00:33:30: 44000000 INFO @ Thu, 16 Apr 2020 00:33:38: 39000000 INFO @ Thu, 16 Apr 2020 00:33:39: 43000000 INFO @ Thu, 16 Apr 2020 00:33:39: 45000000 INFO @ Thu, 16 Apr 2020 00:33:49: 40000000 INFO @ Thu, 16 Apr 2020 00:33:50: 44000000 INFO @ Thu, 16 Apr 2020 00:33:50: 46000000 INFO @ Thu, 16 Apr 2020 00:34:01: 47000000 INFO @ Thu, 16 Apr 2020 00:34:02: 41000000 INFO @ Thu, 16 Apr 2020 00:34:02: 45000000 INFO @ Thu, 16 Apr 2020 00:34:13: 48000000 INFO @ Thu, 16 Apr 2020 00:34:14: 46000000 INFO @ Thu, 16 Apr 2020 00:34:14: 42000000 INFO @ Thu, 16 Apr 2020 00:34:18: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:34:18: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:34:18: #1 total tags in treatment: 16701996 INFO @ Thu, 16 Apr 2020 00:34:18: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:34:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:34:18: #1 tags after filtering in treatment: 13303801 INFO @ Thu, 16 Apr 2020 00:34:18: #1 Redundant rate of treatment: 0.20 INFO @ Thu, 16 Apr 2020 00:34:18: #1 finished! INFO @ Thu, 16 Apr 2020 00:34:18: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:34:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:34:19: #2 number of paired peaks: 314 WARNING @ Thu, 16 Apr 2020 00:34:19: Fewer paired peaks (314) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 314 pairs to build model! INFO @ Thu, 16 Apr 2020 00:34:19: start model_add_line... INFO @ Thu, 16 Apr 2020 00:34:19: start X-correlation... INFO @ Thu, 16 Apr 2020 00:34:19: end of X-cor INFO @ Thu, 16 Apr 2020 00:34:19: #2 finished! INFO @ Thu, 16 Apr 2020 00:34:19: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:34:19: #2 alternative fragment length(s) may be 4,201 bps INFO @ Thu, 16 Apr 2020 00:34:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.20_model.r WARNING @ Thu, 16 Apr 2020 00:34:19: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:34:19: #2 You may need to consider one of the other alternative d(s): 4,201 WARNING @ Thu, 16 Apr 2020 00:34:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:34:19: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:34:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:34:26: 47000000 INFO @ Thu, 16 Apr 2020 00:34:26: 43000000 INFO @ Thu, 16 Apr 2020 00:34:38: 48000000 INFO @ Thu, 16 Apr 2020 00:34:39: 44000000 INFO @ Thu, 16 Apr 2020 00:34:43: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:34:43: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:34:43: #1 total tags in treatment: 16701996 INFO @ Thu, 16 Apr 2020 00:34:43: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:34:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:34:43: #1 tags after filtering in treatment: 13303801 INFO @ Thu, 16 Apr 2020 00:34:43: #1 Redundant rate of treatment: 0.20 INFO @ Thu, 16 Apr 2020 00:34:43: #1 finished! INFO @ Thu, 16 Apr 2020 00:34:43: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:34:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:34:44: #2 number of paired peaks: 314 WARNING @ Thu, 16 Apr 2020 00:34:44: Fewer paired peaks (314) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 314 pairs to build model! INFO @ Thu, 16 Apr 2020 00:34:44: start model_add_line... INFO @ Thu, 16 Apr 2020 00:34:44: start X-correlation... INFO @ Thu, 16 Apr 2020 00:34:44: end of X-cor INFO @ Thu, 16 Apr 2020 00:34:44: #2 finished! INFO @ Thu, 16 Apr 2020 00:34:44: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:34:44: #2 alternative fragment length(s) may be 4,201 bps INFO @ Thu, 16 Apr 2020 00:34:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.05_model.r WARNING @ Thu, 16 Apr 2020 00:34:44: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:34:44: #2 You may need to consider one of the other alternative d(s): 4,201 WARNING @ Thu, 16 Apr 2020 00:34:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:34:44: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:34:44: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:34:47: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:34:50: 45000000 INFO @ Thu, 16 Apr 2020 00:35:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:35:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:35:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.20_summits.bed INFO @ Thu, 16 Apr 2020 00:35:02: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (209 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:35:03: 46000000 INFO @ Thu, 16 Apr 2020 00:35:12: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:35:17: 47000000 INFO @ Thu, 16 Apr 2020 00:35:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:35:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:35:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.05_summits.bed INFO @ Thu, 16 Apr 2020 00:35:28: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (512 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:35:30: 48000000 INFO @ Thu, 16 Apr 2020 00:35:34: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:35:34: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:35:34: #1 total tags in treatment: 16701996 INFO @ Thu, 16 Apr 2020 00:35:34: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:35:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:35:35: #1 tags after filtering in treatment: 13303801 INFO @ Thu, 16 Apr 2020 00:35:35: #1 Redundant rate of treatment: 0.20 INFO @ Thu, 16 Apr 2020 00:35:35: #1 finished! INFO @ Thu, 16 Apr 2020 00:35:35: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:35:35: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:35:36: #2 number of paired peaks: 314 WARNING @ Thu, 16 Apr 2020 00:35:36: Fewer paired peaks (314) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 314 pairs to build model! INFO @ Thu, 16 Apr 2020 00:35:36: start model_add_line... INFO @ Thu, 16 Apr 2020 00:35:36: start X-correlation... INFO @ Thu, 16 Apr 2020 00:35:36: end of X-cor INFO @ Thu, 16 Apr 2020 00:35:36: #2 finished! INFO @ Thu, 16 Apr 2020 00:35:36: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:35:36: #2 alternative fragment length(s) may be 4,201 bps INFO @ Thu, 16 Apr 2020 00:35:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.10_model.r WARNING @ Thu, 16 Apr 2020 00:35:36: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:35:36: #2 You may need to consider one of the other alternative d(s): 4,201 WARNING @ Thu, 16 Apr 2020 00:35:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:35:36: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:35:36: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:36:04: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:36:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:36:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:36:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619548/SRX6619548.10_summits.bed INFO @ Thu, 16 Apr 2020 00:36:19: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (341 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。