Job ID = 5720164 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:26:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 39,751,115 reads read : 79,502,230 reads written : 79,502,230 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:36 39751115 reads; of these: 39751115 (100.00%) were paired; of these: 24879585 (62.59%) aligned concordantly 0 times 12264385 (30.85%) aligned concordantly exactly 1 time 2607145 (6.56%) aligned concordantly >1 times ---- 24879585 pairs aligned concordantly 0 times; of these: 12310378 (49.48%) aligned discordantly 1 time ---- 12569207 pairs aligned 0 times concordantly or discordantly; of these: 25138414 mates make up the pairs; of these: 21858404 (86.95%) aligned 0 times 1138497 (4.53%) aligned exactly 1 time 2141513 (8.52%) aligned >1 times 72.51% overall alignment rate Time searching: 01:09:36 Overall time: 01:09:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4154003 / 26637523 = 0.1559 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:03:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:03:50: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:03:50: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:03:58: 1000000 INFO @ Thu, 16 Apr 2020 00:04:06: 2000000 INFO @ Thu, 16 Apr 2020 00:04:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:04:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:04:20: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:04:20: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:04:24: 4000000 INFO @ Thu, 16 Apr 2020 00:04:30: 1000000 INFO @ Thu, 16 Apr 2020 00:04:33: 5000000 INFO @ Thu, 16 Apr 2020 00:04:40: 2000000 INFO @ Thu, 16 Apr 2020 00:04:43: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:04:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:04:50: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:04:50: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:04:51: 3000000 INFO @ Thu, 16 Apr 2020 00:04:54: 7000000 INFO @ Thu, 16 Apr 2020 00:05:00: 1000000 INFO @ Thu, 16 Apr 2020 00:05:01: 4000000 INFO @ Thu, 16 Apr 2020 00:05:04: 8000000 INFO @ Thu, 16 Apr 2020 00:05:11: 2000000 INFO @ Thu, 16 Apr 2020 00:05:12: 5000000 INFO @ Thu, 16 Apr 2020 00:05:14: 9000000 INFO @ Thu, 16 Apr 2020 00:05:21: 3000000 INFO @ Thu, 16 Apr 2020 00:05:23: 6000000 INFO @ Thu, 16 Apr 2020 00:05:24: 10000000 INFO @ Thu, 16 Apr 2020 00:05:32: 4000000 INFO @ Thu, 16 Apr 2020 00:05:33: 7000000 INFO @ Thu, 16 Apr 2020 00:05:34: 11000000 INFO @ Thu, 16 Apr 2020 00:05:42: 5000000 INFO @ Thu, 16 Apr 2020 00:05:43: 8000000 INFO @ Thu, 16 Apr 2020 00:05:44: 12000000 INFO @ Thu, 16 Apr 2020 00:05:53: 6000000 INFO @ Thu, 16 Apr 2020 00:05:54: 9000000 INFO @ Thu, 16 Apr 2020 00:05:55: 13000000 INFO @ Thu, 16 Apr 2020 00:06:04: 7000000 INFO @ Thu, 16 Apr 2020 00:06:04: 10000000 INFO @ Thu, 16 Apr 2020 00:06:05: 14000000 INFO @ Thu, 16 Apr 2020 00:06:14: 8000000 INFO @ Thu, 16 Apr 2020 00:06:15: 11000000 INFO @ Thu, 16 Apr 2020 00:06:15: 15000000 INFO @ Thu, 16 Apr 2020 00:06:25: 12000000 INFO @ Thu, 16 Apr 2020 00:06:25: 9000000 INFO @ Thu, 16 Apr 2020 00:06:26: 16000000 INFO @ Thu, 16 Apr 2020 00:06:36: 13000000 INFO @ Thu, 16 Apr 2020 00:06:36: 17000000 INFO @ Thu, 16 Apr 2020 00:06:36: 10000000 INFO @ Thu, 16 Apr 2020 00:06:46: 18000000 INFO @ Thu, 16 Apr 2020 00:06:46: 14000000 INFO @ Thu, 16 Apr 2020 00:06:47: 11000000 INFO @ Thu, 16 Apr 2020 00:06:57: 19000000 INFO @ Thu, 16 Apr 2020 00:06:57: 15000000 INFO @ Thu, 16 Apr 2020 00:06:58: 12000000 INFO @ Thu, 16 Apr 2020 00:07:07: 20000000 INFO @ Thu, 16 Apr 2020 00:07:07: 16000000 INFO @ Thu, 16 Apr 2020 00:07:08: 13000000 INFO @ Thu, 16 Apr 2020 00:07:17: 21000000 INFO @ Thu, 16 Apr 2020 00:07:18: 17000000 INFO @ Thu, 16 Apr 2020 00:07:19: 14000000 INFO @ Thu, 16 Apr 2020 00:07:27: 22000000 INFO @ Thu, 16 Apr 2020 00:07:28: 18000000 INFO @ Thu, 16 Apr 2020 00:07:30: 15000000 INFO @ Thu, 16 Apr 2020 00:07:37: 23000000 INFO @ Thu, 16 Apr 2020 00:07:39: 19000000 INFO @ Thu, 16 Apr 2020 00:07:40: 16000000 INFO @ Thu, 16 Apr 2020 00:07:48: 24000000 INFO @ Thu, 16 Apr 2020 00:07:49: 20000000 INFO @ Thu, 16 Apr 2020 00:07:51: 17000000 INFO @ Thu, 16 Apr 2020 00:07:58: 25000000 INFO @ Thu, 16 Apr 2020 00:07:59: 21000000 INFO @ Thu, 16 Apr 2020 00:08:02: 18000000 INFO @ Thu, 16 Apr 2020 00:08:08: 26000000 INFO @ Thu, 16 Apr 2020 00:08:10: 22000000 INFO @ Thu, 16 Apr 2020 00:08:12: 19000000 INFO @ Thu, 16 Apr 2020 00:08:18: 27000000 INFO @ Thu, 16 Apr 2020 00:08:20: 23000000 INFO @ Thu, 16 Apr 2020 00:08:23: 20000000 INFO @ Thu, 16 Apr 2020 00:08:29: 28000000 INFO @ Thu, 16 Apr 2020 00:08:31: 24000000 INFO @ Thu, 16 Apr 2020 00:08:34: 21000000 INFO @ Thu, 16 Apr 2020 00:08:39: 29000000 INFO @ Thu, 16 Apr 2020 00:08:41: 25000000 INFO @ Thu, 16 Apr 2020 00:08:44: 22000000 INFO @ Thu, 16 Apr 2020 00:08:49: 30000000 INFO @ Thu, 16 Apr 2020 00:08:52: 26000000 INFO @ Thu, 16 Apr 2020 00:08:55: 23000000 INFO @ Thu, 16 Apr 2020 00:08:59: 31000000 INFO @ Thu, 16 Apr 2020 00:09:02: 27000000 INFO @ Thu, 16 Apr 2020 00:09:06: 24000000 INFO @ Thu, 16 Apr 2020 00:09:09: 32000000 INFO @ Thu, 16 Apr 2020 00:09:13: 28000000 INFO @ Thu, 16 Apr 2020 00:09:17: 25000000 INFO @ Thu, 16 Apr 2020 00:09:20: 33000000 INFO @ Thu, 16 Apr 2020 00:09:24: 29000000 INFO @ Thu, 16 Apr 2020 00:09:28: 26000000 INFO @ Thu, 16 Apr 2020 00:09:30: 34000000 INFO @ Thu, 16 Apr 2020 00:09:35: 30000000 INFO @ Thu, 16 Apr 2020 00:09:39: 27000000 INFO @ Thu, 16 Apr 2020 00:09:40: 35000000 INFO @ Thu, 16 Apr 2020 00:09:45: 31000000 INFO @ Thu, 16 Apr 2020 00:09:50: 28000000 INFO @ Thu, 16 Apr 2020 00:09:50: 36000000 INFO @ Thu, 16 Apr 2020 00:09:55: 32000000 INFO @ Thu, 16 Apr 2020 00:10:00: 37000000 INFO @ Thu, 16 Apr 2020 00:10:01: 29000000 INFO @ Thu, 16 Apr 2020 00:10:06: 33000000 INFO @ Thu, 16 Apr 2020 00:10:10: 38000000 INFO @ Thu, 16 Apr 2020 00:10:11: 30000000 INFO @ Thu, 16 Apr 2020 00:10:16: 34000000 INFO @ Thu, 16 Apr 2020 00:10:20: 39000000 INFO @ Thu, 16 Apr 2020 00:10:22: 31000000 INFO @ Thu, 16 Apr 2020 00:10:26: 35000000 INFO @ Thu, 16 Apr 2020 00:10:31: 40000000 INFO @ Thu, 16 Apr 2020 00:10:32: 32000000 INFO @ Thu, 16 Apr 2020 00:10:36: 36000000 INFO @ Thu, 16 Apr 2020 00:10:41: 41000000 INFO @ Thu, 16 Apr 2020 00:10:43: 33000000 INFO @ Thu, 16 Apr 2020 00:10:47: 37000000 INFO @ Thu, 16 Apr 2020 00:10:51: 42000000 INFO @ Thu, 16 Apr 2020 00:10:53: 34000000 INFO @ Thu, 16 Apr 2020 00:10:58: 38000000 INFO @ Thu, 16 Apr 2020 00:11:01: 43000000 INFO @ Thu, 16 Apr 2020 00:11:04: 35000000 INFO @ Thu, 16 Apr 2020 00:11:08: 39000000 INFO @ Thu, 16 Apr 2020 00:11:11: 44000000 INFO @ Thu, 16 Apr 2020 00:11:15: 36000000 INFO @ Thu, 16 Apr 2020 00:11:18: 40000000 INFO @ Thu, 16 Apr 2020 00:11:21: 45000000 INFO @ Thu, 16 Apr 2020 00:11:26: 37000000 INFO @ Thu, 16 Apr 2020 00:11:29: 41000000 INFO @ Thu, 16 Apr 2020 00:11:31: 46000000 INFO @ Thu, 16 Apr 2020 00:11:36: 38000000 INFO @ Thu, 16 Apr 2020 00:11:39: 42000000 INFO @ Thu, 16 Apr 2020 00:11:42: 47000000 INFO @ Thu, 16 Apr 2020 00:11:47: 39000000 INFO @ Thu, 16 Apr 2020 00:11:49: 43000000 INFO @ Thu, 16 Apr 2020 00:11:52: 48000000 INFO @ Thu, 16 Apr 2020 00:11:58: 40000000 INFO @ Thu, 16 Apr 2020 00:11:59: 44000000 INFO @ Thu, 16 Apr 2020 00:12:02: 49000000 INFO @ Thu, 16 Apr 2020 00:12:06: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:12:06: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:12:06: #1 total tags in treatment: 12357873 INFO @ Thu, 16 Apr 2020 00:12:06: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:12:06: #1 tags after filtering in treatment: 10443135 INFO @ Thu, 16 Apr 2020 00:12:06: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:12:06: #1 finished! INFO @ Thu, 16 Apr 2020 00:12:06: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:12:07: #2 number of paired peaks: 308 WARNING @ Thu, 16 Apr 2020 00:12:07: Fewer paired peaks (308) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 308 pairs to build model! INFO @ Thu, 16 Apr 2020 00:12:07: start model_add_line... INFO @ Thu, 16 Apr 2020 00:12:07: start X-correlation... INFO @ Thu, 16 Apr 2020 00:12:07: end of X-cor INFO @ Thu, 16 Apr 2020 00:12:07: #2 finished! INFO @ Thu, 16 Apr 2020 00:12:07: #2 predicted fragment length is 218 bps INFO @ Thu, 16 Apr 2020 00:12:07: #2 alternative fragment length(s) may be 218 bps INFO @ Thu, 16 Apr 2020 00:12:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.05_model.r WARNING @ Thu, 16 Apr 2020 00:12:07: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:12:07: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Thu, 16 Apr 2020 00:12:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:12:07: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:12:07: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:12:08: 41000000 INFO @ Thu, 16 Apr 2020 00:12:10: 45000000 INFO @ Thu, 16 Apr 2020 00:12:19: 42000000 INFO @ Thu, 16 Apr 2020 00:12:20: 46000000 INFO @ Thu, 16 Apr 2020 00:12:29: 43000000 INFO @ Thu, 16 Apr 2020 00:12:30: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:12:31: 47000000 INFO @ Thu, 16 Apr 2020 00:12:40: 44000000 INFO @ Thu, 16 Apr 2020 00:12:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:12:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:12:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.05_summits.bed INFO @ Thu, 16 Apr 2020 00:12:41: Done! INFO @ Thu, 16 Apr 2020 00:12:41: 48000000 pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (513 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:12:50: 45000000 INFO @ Thu, 16 Apr 2020 00:12:51: 49000000 INFO @ Thu, 16 Apr 2020 00:12:54: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:12:54: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:12:54: #1 total tags in treatment: 12357873 INFO @ Thu, 16 Apr 2020 00:12:54: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:12:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:12:55: #1 tags after filtering in treatment: 10443135 INFO @ Thu, 16 Apr 2020 00:12:55: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:12:55: #1 finished! INFO @ Thu, 16 Apr 2020 00:12:55: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:12:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:12:55: #2 number of paired peaks: 308 WARNING @ Thu, 16 Apr 2020 00:12:55: Fewer paired peaks (308) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 308 pairs to build model! INFO @ Thu, 16 Apr 2020 00:12:55: start model_add_line... INFO @ Thu, 16 Apr 2020 00:12:56: start X-correlation... INFO @ Thu, 16 Apr 2020 00:12:56: end of X-cor INFO @ Thu, 16 Apr 2020 00:12:56: #2 finished! INFO @ Thu, 16 Apr 2020 00:12:56: #2 predicted fragment length is 218 bps INFO @ Thu, 16 Apr 2020 00:12:56: #2 alternative fragment length(s) may be 218 bps INFO @ Thu, 16 Apr 2020 00:12:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.10_model.r WARNING @ Thu, 16 Apr 2020 00:12:56: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:12:56: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Thu, 16 Apr 2020 00:12:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:12:56: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:12:56: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:13:00: 46000000 INFO @ Thu, 16 Apr 2020 00:13:09: 47000000 INFO @ Thu, 16 Apr 2020 00:13:18: 48000000 INFO @ Thu, 16 Apr 2020 00:13:19: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:13:27: 49000000 INFO @ Thu, 16 Apr 2020 00:13:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:13:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:13:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.10_summits.bed INFO @ Thu, 16 Apr 2020 00:13:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (343 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:13:31: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:13:31: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:13:31: #1 total tags in treatment: 12357873 INFO @ Thu, 16 Apr 2020 00:13:31: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:13:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:13:31: #1 tags after filtering in treatment: 10443135 INFO @ Thu, 16 Apr 2020 00:13:31: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:13:31: #1 finished! INFO @ Thu, 16 Apr 2020 00:13:31: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:13:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:13:32: #2 number of paired peaks: 308 WARNING @ Thu, 16 Apr 2020 00:13:32: Fewer paired peaks (308) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 308 pairs to build model! INFO @ Thu, 16 Apr 2020 00:13:32: start model_add_line... INFO @ Thu, 16 Apr 2020 00:13:32: start X-correlation... INFO @ Thu, 16 Apr 2020 00:13:32: end of X-cor INFO @ Thu, 16 Apr 2020 00:13:32: #2 finished! INFO @ Thu, 16 Apr 2020 00:13:32: #2 predicted fragment length is 218 bps INFO @ Thu, 16 Apr 2020 00:13:32: #2 alternative fragment length(s) may be 218 bps INFO @ Thu, 16 Apr 2020 00:13:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.20_model.r WARNING @ Thu, 16 Apr 2020 00:13:32: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:13:32: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Thu, 16 Apr 2020 00:13:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:13:32: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:13:32: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:13:55: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:14:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:14:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:14:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619535/SRX6619535.20_summits.bed INFO @ Thu, 16 Apr 2020 00:14:05: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (214 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。