Job ID = 5720154 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,940,672 reads read : 37,881,344 reads written : 18,940,672 reads 0-length : 18,940,672 spots read : 50,398,183 reads read : 100,796,366 reads written : 50,398,183 reads 0-length : 50,398,183 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:28 69338855 reads; of these: 69338855 (100.00%) were unpaired; of these: 24277072 (35.01%) aligned 0 times 36850014 (53.14%) aligned exactly 1 time 8211769 (11.84%) aligned >1 times 64.99% overall alignment rate Time searching: 00:18:28 Overall time: 00:18:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 21594653 / 45061783 = 0.4792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 22:14:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 22:14:31: #1 read tag files... INFO @ Wed, 15 Apr 2020 22:14:31: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 22:14:38: 1000000 INFO @ Wed, 15 Apr 2020 22:14:45: 2000000 INFO @ Wed, 15 Apr 2020 22:14:51: 3000000 INFO @ Wed, 15 Apr 2020 22:14:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 22:15:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 22:15:00: #1 read tag files... INFO @ Wed, 15 Apr 2020 22:15:00: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 22:15:03: 5000000 INFO @ Wed, 15 Apr 2020 22:15:07: 1000000 INFO @ Wed, 15 Apr 2020 22:15:09: 6000000 INFO @ Wed, 15 Apr 2020 22:15:13: 2000000 INFO @ Wed, 15 Apr 2020 22:15:16: 7000000 INFO @ Wed, 15 Apr 2020 22:15:20: 3000000 INFO @ Wed, 15 Apr 2020 22:15:23: 8000000 INFO @ Wed, 15 Apr 2020 22:15:27: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 22:15:28: 9000000 INFO @ Wed, 15 Apr 2020 22:15:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 22:15:30: #1 read tag files... INFO @ Wed, 15 Apr 2020 22:15:30: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 22:15:34: 10000000 INFO @ Wed, 15 Apr 2020 22:15:34: 5000000 INFO @ Wed, 15 Apr 2020 22:15:38: 1000000 INFO @ Wed, 15 Apr 2020 22:15:40: 11000000 INFO @ Wed, 15 Apr 2020 22:15:41: 6000000 INFO @ Wed, 15 Apr 2020 22:15:45: 2000000 INFO @ Wed, 15 Apr 2020 22:15:46: 12000000 INFO @ Wed, 15 Apr 2020 22:15:50: 7000000 INFO @ Wed, 15 Apr 2020 22:15:52: 13000000 INFO @ Wed, 15 Apr 2020 22:15:53: 3000000 INFO @ Wed, 15 Apr 2020 22:15:57: 8000000 INFO @ Wed, 15 Apr 2020 22:15:58: 14000000 INFO @ Wed, 15 Apr 2020 22:16:01: 4000000 INFO @ Wed, 15 Apr 2020 22:16:03: 15000000 INFO @ Wed, 15 Apr 2020 22:16:04: 9000000 INFO @ Wed, 15 Apr 2020 22:16:08: 5000000 INFO @ Wed, 15 Apr 2020 22:16:10: 16000000 INFO @ Wed, 15 Apr 2020 22:16:12: 10000000 INFO @ Wed, 15 Apr 2020 22:16:15: 6000000 INFO @ Wed, 15 Apr 2020 22:16:16: 17000000 INFO @ Wed, 15 Apr 2020 22:16:19: 11000000 INFO @ Wed, 15 Apr 2020 22:16:22: 18000000 INFO @ Wed, 15 Apr 2020 22:16:23: 7000000 INFO @ Wed, 15 Apr 2020 22:16:27: 12000000 INFO @ Wed, 15 Apr 2020 22:16:27: 19000000 INFO @ Wed, 15 Apr 2020 22:16:31: 8000000 INFO @ Wed, 15 Apr 2020 22:16:33: 20000000 INFO @ Wed, 15 Apr 2020 22:16:34: 13000000 INFO @ Wed, 15 Apr 2020 22:16:38: 9000000 INFO @ Wed, 15 Apr 2020 22:16:39: 21000000 INFO @ Wed, 15 Apr 2020 22:16:41: 14000000 INFO @ Wed, 15 Apr 2020 22:16:46: 22000000 INFO @ Wed, 15 Apr 2020 22:16:46: 10000000 INFO @ Wed, 15 Apr 2020 22:16:48: 15000000 INFO @ Wed, 15 Apr 2020 22:16:52: 23000000 INFO @ Wed, 15 Apr 2020 22:16:54: 11000000 INFO @ Wed, 15 Apr 2020 22:16:55: #1 tag size is determined as 55 bps INFO @ Wed, 15 Apr 2020 22:16:55: #1 tag size = 55 INFO @ Wed, 15 Apr 2020 22:16:55: #1 total tags in treatment: 23467130 INFO @ Wed, 15 Apr 2020 22:16:55: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 22:16:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 22:16:55: #1 tags after filtering in treatment: 23467130 INFO @ Wed, 15 Apr 2020 22:16:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 22:16:55: #1 finished! INFO @ Wed, 15 Apr 2020 22:16:55: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 22:16:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 22:16:56: 16000000 INFO @ Wed, 15 Apr 2020 22:16:57: #2 number of paired peaks: 96 WARNING @ Wed, 15 Apr 2020 22:16:57: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 22:16:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 22:17:02: 12000000 INFO @ Wed, 15 Apr 2020 22:17:03: 17000000 INFO @ Wed, 15 Apr 2020 22:17:09: 13000000 INFO @ Wed, 15 Apr 2020 22:17:10: 18000000 INFO @ Wed, 15 Apr 2020 22:17:16: 14000000 INFO @ Wed, 15 Apr 2020 22:17:17: 19000000 INFO @ Wed, 15 Apr 2020 22:17:24: 15000000 INFO @ Wed, 15 Apr 2020 22:17:24: 20000000 INFO @ Wed, 15 Apr 2020 22:17:31: 16000000 INFO @ Wed, 15 Apr 2020 22:17:31: 21000000 INFO @ Wed, 15 Apr 2020 22:17:38: 22000000 INFO @ Wed, 15 Apr 2020 22:17:38: 17000000 INFO @ Wed, 15 Apr 2020 22:17:45: 23000000 INFO @ Wed, 15 Apr 2020 22:17:46: 18000000 INFO @ Wed, 15 Apr 2020 22:17:49: #1 tag size is determined as 55 bps INFO @ Wed, 15 Apr 2020 22:17:49: #1 tag size = 55 INFO @ Wed, 15 Apr 2020 22:17:49: #1 total tags in treatment: 23467130 INFO @ Wed, 15 Apr 2020 22:17:49: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 22:17:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 22:17:50: #1 tags after filtering in treatment: 23467130 INFO @ Wed, 15 Apr 2020 22:17:50: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 22:17:50: #1 finished! INFO @ Wed, 15 Apr 2020 22:17:50: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 22:17:50: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 22:17:51: #2 number of paired peaks: 96 WARNING @ Wed, 15 Apr 2020 22:17:51: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 22:17:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 22:17:53: 19000000 INFO @ Wed, 15 Apr 2020 22:17:59: 20000000 INFO @ Wed, 15 Apr 2020 22:18:05: 21000000 INFO @ Wed, 15 Apr 2020 22:18:11: 22000000 INFO @ Wed, 15 Apr 2020 22:18:19: 23000000 INFO @ Wed, 15 Apr 2020 22:18:22: #1 tag size is determined as 55 bps INFO @ Wed, 15 Apr 2020 22:18:22: #1 tag size = 55 INFO @ Wed, 15 Apr 2020 22:18:22: #1 total tags in treatment: 23467130 INFO @ Wed, 15 Apr 2020 22:18:22: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 22:18:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 22:18:22: #1 tags after filtering in treatment: 23467130 INFO @ Wed, 15 Apr 2020 22:18:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 22:18:22: #1 finished! INFO @ Wed, 15 Apr 2020 22:18:22: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 22:18:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 22:18:24: #2 number of paired peaks: 96 WARNING @ Wed, 15 Apr 2020 22:18:24: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 22:18:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702597/SRX5702597.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。