Job ID = 5720151 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,723,506 reads read : 47,447,012 reads written : 23,723,506 reads 0-length : 23,723,506 spots read : 20,111,351 reads read : 40,222,702 reads written : 20,111,351 reads 0-length : 20,111,351 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:42 43834857 reads; of these: 43834857 (100.00%) were unpaired; of these: 807972 (1.84%) aligned 0 times 36090108 (82.33%) aligned exactly 1 time 6936777 (15.82%) aligned >1 times 98.16% overall alignment rate Time searching: 00:08:42 Overall time: 00:08:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12085906 / 43026885 = 0.2809 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 21:42:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 21:42:06: #1 read tag files... INFO @ Wed, 15 Apr 2020 21:42:06: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 21:42:11: 1000000 INFO @ Wed, 15 Apr 2020 21:42:16: 2000000 INFO @ Wed, 15 Apr 2020 21:42:20: 3000000 INFO @ Wed, 15 Apr 2020 21:42:25: 4000000 INFO @ Wed, 15 Apr 2020 21:42:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 21:42:34: 6000000 INFO @ Wed, 15 Apr 2020 21:42:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 21:42:35: #1 read tag files... INFO @ Wed, 15 Apr 2020 21:42:35: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 21:42:39: 7000000 INFO @ Wed, 15 Apr 2020 21:42:40: 1000000 INFO @ Wed, 15 Apr 2020 21:42:44: 8000000 INFO @ Wed, 15 Apr 2020 21:42:45: 2000000 INFO @ Wed, 15 Apr 2020 21:42:49: 9000000 INFO @ Wed, 15 Apr 2020 21:42:50: 3000000 INFO @ Wed, 15 Apr 2020 21:42:54: 10000000 INFO @ Wed, 15 Apr 2020 21:42:55: 4000000 INFO @ Wed, 15 Apr 2020 21:42:59: 11000000 INFO @ Wed, 15 Apr 2020 21:43:00: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 21:43:03: 12000000 INFO @ Wed, 15 Apr 2020 21:43:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 21:43:04: #1 read tag files... INFO @ Wed, 15 Apr 2020 21:43:04: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 21:43:05: 6000000 INFO @ Wed, 15 Apr 2020 21:43:08: 13000000 INFO @ Wed, 15 Apr 2020 21:43:09: 1000000 INFO @ Wed, 15 Apr 2020 21:43:10: 7000000 INFO @ Wed, 15 Apr 2020 21:43:13: 14000000 INFO @ Wed, 15 Apr 2020 21:43:14: 2000000 INFO @ Wed, 15 Apr 2020 21:43:15: 8000000 INFO @ Wed, 15 Apr 2020 21:43:18: 15000000 INFO @ Wed, 15 Apr 2020 21:43:19: 3000000 INFO @ Wed, 15 Apr 2020 21:43:20: 9000000 INFO @ Wed, 15 Apr 2020 21:43:23: 16000000 INFO @ Wed, 15 Apr 2020 21:43:24: 4000000 INFO @ Wed, 15 Apr 2020 21:43:25: 10000000 INFO @ Wed, 15 Apr 2020 21:43:28: 17000000 INFO @ Wed, 15 Apr 2020 21:43:29: 5000000 INFO @ Wed, 15 Apr 2020 21:43:30: 11000000 INFO @ Wed, 15 Apr 2020 21:43:33: 18000000 INFO @ Wed, 15 Apr 2020 21:43:34: 6000000 INFO @ Wed, 15 Apr 2020 21:43:35: 12000000 INFO @ Wed, 15 Apr 2020 21:43:38: 7000000 INFO @ Wed, 15 Apr 2020 21:43:39: 19000000 INFO @ Wed, 15 Apr 2020 21:43:40: 13000000 INFO @ Wed, 15 Apr 2020 21:43:43: 8000000 INFO @ Wed, 15 Apr 2020 21:43:44: 20000000 INFO @ Wed, 15 Apr 2020 21:43:45: 14000000 INFO @ Wed, 15 Apr 2020 21:43:48: 9000000 INFO @ Wed, 15 Apr 2020 21:43:48: 21000000 INFO @ Wed, 15 Apr 2020 21:43:50: 15000000 INFO @ Wed, 15 Apr 2020 21:43:53: 10000000 INFO @ Wed, 15 Apr 2020 21:43:53: 22000000 INFO @ Wed, 15 Apr 2020 21:43:55: 16000000 INFO @ Wed, 15 Apr 2020 21:43:58: 11000000 INFO @ Wed, 15 Apr 2020 21:43:58: 23000000 INFO @ Wed, 15 Apr 2020 21:44:00: 17000000 INFO @ Wed, 15 Apr 2020 21:44:03: 12000000 INFO @ Wed, 15 Apr 2020 21:44:03: 24000000 INFO @ Wed, 15 Apr 2020 21:44:05: 18000000 INFO @ Wed, 15 Apr 2020 21:44:08: 13000000 INFO @ Wed, 15 Apr 2020 21:44:08: 25000000 INFO @ Wed, 15 Apr 2020 21:44:10: 19000000 INFO @ Wed, 15 Apr 2020 21:44:12: 14000000 INFO @ Wed, 15 Apr 2020 21:44:13: 26000000 INFO @ Wed, 15 Apr 2020 21:44:15: 20000000 INFO @ Wed, 15 Apr 2020 21:44:17: 15000000 INFO @ Wed, 15 Apr 2020 21:44:19: 27000000 INFO @ Wed, 15 Apr 2020 21:44:20: 21000000 INFO @ Wed, 15 Apr 2020 21:44:23: 16000000 INFO @ Wed, 15 Apr 2020 21:44:25: 22000000 INFO @ Wed, 15 Apr 2020 21:44:26: 28000000 INFO @ Wed, 15 Apr 2020 21:44:28: 17000000 INFO @ Wed, 15 Apr 2020 21:44:30: 23000000 INFO @ Wed, 15 Apr 2020 21:44:31: 29000000 INFO @ Wed, 15 Apr 2020 21:44:33: 18000000 INFO @ Wed, 15 Apr 2020 21:44:35: 24000000 INFO @ Wed, 15 Apr 2020 21:44:36: 30000000 INFO @ Wed, 15 Apr 2020 21:44:38: 19000000 INFO @ Wed, 15 Apr 2020 21:44:41: 25000000 INFO @ Wed, 15 Apr 2020 21:44:41: #1 tag size is determined as 50 bps INFO @ Wed, 15 Apr 2020 21:44:41: #1 tag size = 50 INFO @ Wed, 15 Apr 2020 21:44:41: #1 total tags in treatment: 30940979 INFO @ Wed, 15 Apr 2020 21:44:41: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 21:44:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 21:44:41: #1 tags after filtering in treatment: 30940979 INFO @ Wed, 15 Apr 2020 21:44:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 21:44:41: #1 finished! INFO @ Wed, 15 Apr 2020 21:44:41: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 21:44:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 21:44:43: #2 number of paired peaks: 46 WARNING @ Wed, 15 Apr 2020 21:44:43: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 21:44:43: Process for pairing-model is terminated! INFO @ Wed, 15 Apr 2020 21:44:43: 20000000 INFO @ Wed, 15 Apr 2020 21:44:46: 26000000 cut: /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 21:44:48: 21000000 INFO @ Wed, 15 Apr 2020 21:44:51: 27000000 INFO @ Wed, 15 Apr 2020 21:44:53: 22000000 INFO @ Wed, 15 Apr 2020 21:44:56: 28000000 INFO @ Wed, 15 Apr 2020 21:44:58: 23000000 INFO @ Wed, 15 Apr 2020 21:45:01: 29000000 INFO @ Wed, 15 Apr 2020 21:45:03: 24000000 INFO @ Wed, 15 Apr 2020 21:45:06: 30000000 INFO @ Wed, 15 Apr 2020 21:45:08: 25000000 INFO @ Wed, 15 Apr 2020 21:45:11: #1 tag size is determined as 50 bps INFO @ Wed, 15 Apr 2020 21:45:11: #1 tag size = 50 INFO @ Wed, 15 Apr 2020 21:45:11: #1 total tags in treatment: 30940979 INFO @ Wed, 15 Apr 2020 21:45:11: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 21:45:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 21:45:11: #1 tags after filtering in treatment: 30940979 INFO @ Wed, 15 Apr 2020 21:45:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 21:45:11: #1 finished! INFO @ Wed, 15 Apr 2020 21:45:11: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 21:45:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 21:45:13: 26000000 INFO @ Wed, 15 Apr 2020 21:45:13: #2 number of paired peaks: 46 WARNING @ Wed, 15 Apr 2020 21:45:13: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 21:45:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 21:45:18: 27000000 INFO @ Wed, 15 Apr 2020 21:45:22: 28000000 INFO @ Wed, 15 Apr 2020 21:45:27: 29000000 INFO @ Wed, 15 Apr 2020 21:45:32: 30000000 INFO @ Wed, 15 Apr 2020 21:45:37: #1 tag size is determined as 50 bps INFO @ Wed, 15 Apr 2020 21:45:37: #1 tag size = 50 INFO @ Wed, 15 Apr 2020 21:45:37: #1 total tags in treatment: 30940979 INFO @ Wed, 15 Apr 2020 21:45:37: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 21:45:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 21:45:37: #1 tags after filtering in treatment: 30940979 INFO @ Wed, 15 Apr 2020 21:45:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 21:45:37: #1 finished! INFO @ Wed, 15 Apr 2020 21:45:37: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 21:45:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 21:45:39: #2 number of paired peaks: 46 WARNING @ Wed, 15 Apr 2020 21:45:39: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 21:45:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5702594/SRX5702594.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。