Job ID = 2590216 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 749,391 reads read : 1,498,782 reads written : 749,391 reads 0-length : 749,391 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:53 749391 reads; of these: 749391 (100.00%) were unpaired; of these: 749153 (99.97%) aligned 0 times 112 (0.01%) aligned exactly 1 time 126 (0.02%) aligned >1 times 0.03% overall alignment rate Time searching: 00:00:53 Overall time: 00:00:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 202 / 238 = 0.8487 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Mon, 12 Aug 2019 20:05:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:05:23: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:05:23: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:05:23: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 20:05:23: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 20:05:23: #1 total tags in treatment: 36 INFO @ Mon, 12 Aug 2019 20:05:23: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:05:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:05:23: #1 tags after filtering in treatment: 34 INFO @ Mon, 12 Aug 2019 20:05:23: #1 Redundant rate of treatment: 0.06 INFO @ Mon, 12 Aug 2019 20:05:23: #1 finished! INFO @ Mon, 12 Aug 2019 20:05:23: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:05:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:05:23: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:05:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:05:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:05:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:05:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:05:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:05:24: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 20:05:24: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 20:05:24: #1 total tags in treatment: 36 INFO @ Mon, 12 Aug 2019 20:05:24: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:05:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:05:24: #1 tags after filtering in treatment: 34 INFO @ Mon, 12 Aug 2019 20:05:24: #1 Redundant rate of treatment: 0.06 INFO @ Mon, 12 Aug 2019 20:05:24: #1 finished! INFO @ Mon, 12 Aug 2019 20:05:24: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:05:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:05:24: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:05:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:05:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:05:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:05:25: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:05:25: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:05:25: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 20:05:25: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 20:05:25: #1 total tags in treatment: 36 INFO @ Mon, 12 Aug 2019 20:05:25: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:05:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:05:25: #1 tags after filtering in treatment: 34 INFO @ Mon, 12 Aug 2019 20:05:25: #1 Redundant rate of treatment: 0.06 INFO @ Mon, 12 Aug 2019 20:05:25: #1 finished! INFO @ Mon, 12 Aug 2019 20:05:25: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:05:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:05:25: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:05:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:05:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX560680/SRX560680.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling