Job ID = 3785776 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:27:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:31:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:54 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:54 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:35 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 33,377,549 reads read : 66,755,098 reads written : 33,377,549 reads 0-length : 33,377,549 2019-11-01T05:52:35 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:33 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:58:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:02:40 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:04:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 52,862,300 reads read : 105,724,600 reads written : 52,862,300 reads 0-length : 52,862,300 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:38 86239849 reads; of these: 86239849 (100.00%) were unpaired; of these: 24394874 (28.29%) aligned 0 times 51261192 (59.44%) aligned exactly 1 time 10583783 (12.27%) aligned >1 times 71.71% overall alignment rate Time searching: 00:17:39 Overall time: 00:17:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 32897013 / 61844975 = 0.5319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:45:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:45:15: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:45:15: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:45:25: 1000000 INFO @ Fri, 01 Nov 2019 15:45:35: 2000000 INFO @ Fri, 01 Nov 2019 15:45:44: 3000000 INFO @ Fri, 01 Nov 2019 15:45:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:45:45: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:45:45: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:45:54: 1000000 INFO @ Fri, 01 Nov 2019 15:45:55: 4000000 INFO @ Fri, 01 Nov 2019 15:46:02: 2000000 INFO @ Fri, 01 Nov 2019 15:46:05: 5000000 INFO @ Fri, 01 Nov 2019 15:46:12: 3000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:46:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:46:15: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:46:15: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:46:16: 6000000 INFO @ Fri, 01 Nov 2019 15:46:23: 4000000 INFO @ Fri, 01 Nov 2019 15:46:23: 1000000 INFO @ Fri, 01 Nov 2019 15:46:26: 7000000 INFO @ Fri, 01 Nov 2019 15:46:32: 2000000 INFO @ Fri, 01 Nov 2019 15:46:33: 5000000 INFO @ Fri, 01 Nov 2019 15:46:37: 8000000 INFO @ Fri, 01 Nov 2019 15:46:40: 3000000 INFO @ Fri, 01 Nov 2019 15:46:44: 6000000 INFO @ Fri, 01 Nov 2019 15:46:48: 9000000 INFO @ Fri, 01 Nov 2019 15:46:49: 4000000 INFO @ Fri, 01 Nov 2019 15:46:55: 7000000 INFO @ Fri, 01 Nov 2019 15:46:57: 5000000 INFO @ Fri, 01 Nov 2019 15:46:58: 10000000 INFO @ Fri, 01 Nov 2019 15:47:06: 6000000 INFO @ Fri, 01 Nov 2019 15:47:06: 8000000 INFO @ Fri, 01 Nov 2019 15:47:09: 11000000 INFO @ Fri, 01 Nov 2019 15:47:15: 7000000 INFO @ Fri, 01 Nov 2019 15:47:17: 9000000 INFO @ Fri, 01 Nov 2019 15:47:19: 12000000 INFO @ Fri, 01 Nov 2019 15:47:23: 8000000 INFO @ Fri, 01 Nov 2019 15:47:27: 10000000 INFO @ Fri, 01 Nov 2019 15:47:29: 13000000 INFO @ Fri, 01 Nov 2019 15:47:31: 9000000 INFO @ Fri, 01 Nov 2019 15:47:38: 11000000 INFO @ Fri, 01 Nov 2019 15:47:39: 10000000 INFO @ Fri, 01 Nov 2019 15:47:40: 14000000 INFO @ Fri, 01 Nov 2019 15:47:48: 11000000 INFO @ Fri, 01 Nov 2019 15:47:48: 12000000 INFO @ Fri, 01 Nov 2019 15:47:50: 15000000 INFO @ Fri, 01 Nov 2019 15:47:56: 12000000 INFO @ Fri, 01 Nov 2019 15:47:59: 13000000 INFO @ Fri, 01 Nov 2019 15:48:01: 16000000 INFO @ Fri, 01 Nov 2019 15:48:04: 13000000 INFO @ Fri, 01 Nov 2019 15:48:10: 14000000 INFO @ Fri, 01 Nov 2019 15:48:12: 17000000 INFO @ Fri, 01 Nov 2019 15:48:12: 14000000 INFO @ Fri, 01 Nov 2019 15:48:20: 15000000 INFO @ Fri, 01 Nov 2019 15:48:21: 15000000 INFO @ Fri, 01 Nov 2019 15:48:22: 18000000 INFO @ Fri, 01 Nov 2019 15:48:28: 16000000 INFO @ Fri, 01 Nov 2019 15:48:32: 16000000 INFO @ Fri, 01 Nov 2019 15:48:33: 19000000 INFO @ Fri, 01 Nov 2019 15:48:36: 17000000 INFO @ Fri, 01 Nov 2019 15:48:43: 17000000 INFO @ Fri, 01 Nov 2019 15:48:43: 20000000 INFO @ Fri, 01 Nov 2019 15:48:44: 18000000 INFO @ Fri, 01 Nov 2019 15:48:51: 19000000 INFO @ Fri, 01 Nov 2019 15:48:53: 18000000 INFO @ Fri, 01 Nov 2019 15:48:54: 21000000 INFO @ Fri, 01 Nov 2019 15:48:59: 20000000 INFO @ Fri, 01 Nov 2019 15:49:04: 19000000 INFO @ Fri, 01 Nov 2019 15:49:04: 22000000 INFO @ Fri, 01 Nov 2019 15:49:07: 21000000 INFO @ Fri, 01 Nov 2019 15:49:14: 20000000 INFO @ Fri, 01 Nov 2019 15:49:15: 22000000 INFO @ Fri, 01 Nov 2019 15:49:15: 23000000 INFO @ Fri, 01 Nov 2019 15:49:22: 23000000 INFO @ Fri, 01 Nov 2019 15:49:25: 21000000 INFO @ Fri, 01 Nov 2019 15:49:26: 24000000 INFO @ Fri, 01 Nov 2019 15:49:30: 24000000 INFO @ Fri, 01 Nov 2019 15:49:35: 22000000 INFO @ Fri, 01 Nov 2019 15:49:38: 25000000 INFO @ Fri, 01 Nov 2019 15:49:38: 25000000 INFO @ Fri, 01 Nov 2019 15:49:46: 26000000 INFO @ Fri, 01 Nov 2019 15:49:46: 23000000 INFO @ Fri, 01 Nov 2019 15:49:48: 26000000 INFO @ Fri, 01 Nov 2019 15:49:53: 27000000 INFO @ Fri, 01 Nov 2019 15:49:56: 24000000 INFO @ Fri, 01 Nov 2019 15:49:59: 27000000 INFO @ Fri, 01 Nov 2019 15:50:01: 28000000 INFO @ Fri, 01 Nov 2019 15:50:07: 25000000 INFO @ Fri, 01 Nov 2019 15:50:08: #1 tag size is determined as 51 bps INFO @ Fri, 01 Nov 2019 15:50:08: #1 tag size = 51 INFO @ Fri, 01 Nov 2019 15:50:08: #1 total tags in treatment: 28947962 INFO @ Fri, 01 Nov 2019 15:50:08: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:50:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:50:08: #1 tags after filtering in treatment: 28947962 INFO @ Fri, 01 Nov 2019 15:50:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:50:08: #1 finished! INFO @ Fri, 01 Nov 2019 15:50:08: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:50:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:50:09: 28000000 INFO @ Fri, 01 Nov 2019 15:50:11: #2 number of paired peaks: 240 WARNING @ Fri, 01 Nov 2019 15:50:11: Fewer paired peaks (240) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 240 pairs to build model! INFO @ Fri, 01 Nov 2019 15:50:11: start model_add_line... INFO @ Fri, 01 Nov 2019 15:50:11: start X-correlation... INFO @ Fri, 01 Nov 2019 15:50:11: end of X-cor INFO @ Fri, 01 Nov 2019 15:50:11: #2 finished! INFO @ Fri, 01 Nov 2019 15:50:11: #2 predicted fragment length is 2 bps INFO @ Fri, 01 Nov 2019 15:50:11: #2 alternative fragment length(s) may be 2,47 bps INFO @ Fri, 01 Nov 2019 15:50:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.20_model.r WARNING @ Fri, 01 Nov 2019 15:50:11: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:50:11: #2 You may need to consider one of the other alternative d(s): 2,47 WARNING @ Fri, 01 Nov 2019 15:50:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:50:11: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:50:11: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:50:18: 26000000 INFO @ Fri, 01 Nov 2019 15:50:18: #1 tag size is determined as 51 bps INFO @ Fri, 01 Nov 2019 15:50:18: #1 tag size = 51 INFO @ Fri, 01 Nov 2019 15:50:18: #1 total tags in treatment: 28947962 INFO @ Fri, 01 Nov 2019 15:50:18: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:50:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:50:19: #1 tags after filtering in treatment: 28947962 INFO @ Fri, 01 Nov 2019 15:50:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:50:19: #1 finished! INFO @ Fri, 01 Nov 2019 15:50:19: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:50:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:50:21: #2 number of paired peaks: 240 WARNING @ Fri, 01 Nov 2019 15:50:21: Fewer paired peaks (240) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 240 pairs to build model! INFO @ Fri, 01 Nov 2019 15:50:21: start model_add_line... INFO @ Fri, 01 Nov 2019 15:50:22: start X-correlation... INFO @ Fri, 01 Nov 2019 15:50:22: end of X-cor INFO @ Fri, 01 Nov 2019 15:50:22: #2 finished! INFO @ Fri, 01 Nov 2019 15:50:22: #2 predicted fragment length is 2 bps INFO @ Fri, 01 Nov 2019 15:50:22: #2 alternative fragment length(s) may be 2,47 bps INFO @ Fri, 01 Nov 2019 15:50:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.05_model.r WARNING @ Fri, 01 Nov 2019 15:50:22: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:50:22: #2 You may need to consider one of the other alternative d(s): 2,47 WARNING @ Fri, 01 Nov 2019 15:50:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:50:22: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:50:22: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:50:28: 27000000 INFO @ Fri, 01 Nov 2019 15:50:38: 28000000 INFO @ Fri, 01 Nov 2019 15:50:47: #1 tag size is determined as 51 bps INFO @ Fri, 01 Nov 2019 15:50:47: #1 tag size = 51 INFO @ Fri, 01 Nov 2019 15:50:47: #1 total tags in treatment: 28947962 INFO @ Fri, 01 Nov 2019 15:50:47: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:50:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:50:48: #1 tags after filtering in treatment: 28947962 INFO @ Fri, 01 Nov 2019 15:50:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:50:48: #1 finished! INFO @ Fri, 01 Nov 2019 15:50:48: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:50:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:50:50: #2 number of paired peaks: 240 WARNING @ Fri, 01 Nov 2019 15:50:50: Fewer paired peaks (240) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 240 pairs to build model! INFO @ Fri, 01 Nov 2019 15:50:50: start model_add_line... INFO @ Fri, 01 Nov 2019 15:50:50: start X-correlation... INFO @ Fri, 01 Nov 2019 15:50:50: end of X-cor INFO @ Fri, 01 Nov 2019 15:50:50: #2 finished! INFO @ Fri, 01 Nov 2019 15:50:50: #2 predicted fragment length is 2 bps INFO @ Fri, 01 Nov 2019 15:50:50: #2 alternative fragment length(s) may be 2,47 bps INFO @ Fri, 01 Nov 2019 15:50:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.10_model.r WARNING @ Fri, 01 Nov 2019 15:50:50: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:50:50: #2 You may need to consider one of the other alternative d(s): 2,47 WARNING @ Fri, 01 Nov 2019 15:50:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:50:50: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:50:50: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:51:07: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:51:17: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:51:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.20_peaks.xls INFO @ Fri, 01 Nov 2019 15:51:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.20_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:51:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.20_summits.bed INFO @ Fri, 01 Nov 2019 15:51:33: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:51:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.05_peaks.xls INFO @ Fri, 01 Nov 2019 15:51:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.05_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:51:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.05_summits.bed INFO @ Fri, 01 Nov 2019 15:51:42: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:51:46: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:52:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.10_peaks.xls INFO @ Fri, 01 Nov 2019 15:52:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.10_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:52:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5545238/SRX5545238.10_summits.bed INFO @ Fri, 01 Nov 2019 15:52:11: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。