Job ID = 6497430 SRX = SRX494928 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T21:57:38 prefetch.2.10.7: 1) Downloading 'SRR1198460'... 2020-06-25T21:57:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T22:03:26 prefetch.2.10.7: HTTPS download succeed 2020-06-25T22:03:26 prefetch.2.10.7: 1) 'SRR1198460' was downloaded successfully Read 47950615 spots for SRR1198460/SRR1198460.sra Written 47950615 spots for SRR1198460/SRR1198460.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:51 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139845 (6.55%) aligned 0 times 37484934 (78.17%) aligned exactly 1 time 7325836 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8187467 / 44810770 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:28:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:28:30: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:28:30: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:28:36: 1000000 INFO @ Fri, 26 Jun 2020 07:28:42: 2000000 INFO @ Fri, 26 Jun 2020 07:28:48: 3000000 INFO @ Fri, 26 Jun 2020 07:28:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:29:00: 5000000 INFO @ Fri, 26 Jun 2020 07:29:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:29:00: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:29:00: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:29:07: 6000000 INFO @ Fri, 26 Jun 2020 07:29:08: 1000000 INFO @ Fri, 26 Jun 2020 07:29:14: 7000000 INFO @ Fri, 26 Jun 2020 07:29:15: 2000000 INFO @ Fri, 26 Jun 2020 07:29:21: 8000000 INFO @ Fri, 26 Jun 2020 07:29:22: 3000000 INFO @ Fri, 26 Jun 2020 07:29:28: 9000000 BedGraph に変換中... INFO @ Fri, 26 Jun 2020 07:29:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:29:35: 10000000 INFO @ Fri, 26 Jun 2020 07:29:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:29:35: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:29:35: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:29:37: 5000000 INFO @ Fri, 26 Jun 2020 07:29:42: 11000000 INFO @ Fri, 26 Jun 2020 07:29:43: 1000000 INFO @ Fri, 26 Jun 2020 07:29:44: 6000000 INFO @ Fri, 26 Jun 2020 07:29:49: 12000000 INFO @ Fri, 26 Jun 2020 07:29:50: 2000000 INFO @ Fri, 26 Jun 2020 07:29:51: 7000000 INFO @ Fri, 26 Jun 2020 07:29:57: 13000000 INFO @ Fri, 26 Jun 2020 07:29:57: 3000000 INFO @ Fri, 26 Jun 2020 07:29:58: 8000000 INFO @ Fri, 26 Jun 2020 07:30:04: 14000000 INFO @ Fri, 26 Jun 2020 07:30:04: 4000000 INFO @ Fri, 26 Jun 2020 07:30:06: 9000000 INFO @ Fri, 26 Jun 2020 07:30:11: 15000000 INFO @ Fri, 26 Jun 2020 07:30:11: 5000000 INFO @ Fri, 26 Jun 2020 07:30:13: 10000000 INFO @ Fri, 26 Jun 2020 07:30:18: 16000000 INFO @ Fri, 26 Jun 2020 07:30:18: 6000000 INFO @ Fri, 26 Jun 2020 07:30:20: 11000000 INFO @ Fri, 26 Jun 2020 07:30:25: 17000000 INFO @ Fri, 26 Jun 2020 07:30:26: 7000000 INFO @ Fri, 26 Jun 2020 07:30:28: 12000000 INFO @ Fri, 26 Jun 2020 07:30:32: 18000000 INFO @ Fri, 26 Jun 2020 07:30:33: 8000000 INFO @ Fri, 26 Jun 2020 07:30:35: 13000000 INFO @ Fri, 26 Jun 2020 07:30:39: 19000000 INFO @ Fri, 26 Jun 2020 07:30:40: 9000000 INFO @ Fri, 26 Jun 2020 07:30:42: 14000000 INFO @ Fri, 26 Jun 2020 07:30:46: 20000000 INFO @ Fri, 26 Jun 2020 07:30:47: 10000000 INFO @ Fri, 26 Jun 2020 07:30:50: 15000000 INFO @ Fri, 26 Jun 2020 07:30:53: 21000000 INFO @ Fri, 26 Jun 2020 07:30:54: 11000000 INFO @ Fri, 26 Jun 2020 07:30:57: 16000000 INFO @ Fri, 26 Jun 2020 07:31:01: 22000000 INFO @ Fri, 26 Jun 2020 07:31:01: 12000000 INFO @ Fri, 26 Jun 2020 07:31:04: 17000000 INFO @ Fri, 26 Jun 2020 07:31:08: 23000000 INFO @ Fri, 26 Jun 2020 07:31:08: 13000000 INFO @ Fri, 26 Jun 2020 07:31:11: 18000000 INFO @ Fri, 26 Jun 2020 07:31:15: 24000000 INFO @ Fri, 26 Jun 2020 07:31:15: 14000000 INFO @ Fri, 26 Jun 2020 07:31:18: 19000000 INFO @ Fri, 26 Jun 2020 07:31:22: 25000000 INFO @ Fri, 26 Jun 2020 07:31:22: 15000000 INFO @ Fri, 26 Jun 2020 07:31:25: 20000000 INFO @ Fri, 26 Jun 2020 07:31:29: 26000000 INFO @ Fri, 26 Jun 2020 07:31:30: 16000000 INFO @ Fri, 26 Jun 2020 07:31:33: 21000000 INFO @ Fri, 26 Jun 2020 07:31:36: 27000000 INFO @ Fri, 26 Jun 2020 07:31:37: 17000000 INFO @ Fri, 26 Jun 2020 07:31:40: 22000000 INFO @ Fri, 26 Jun 2020 07:31:43: 28000000 INFO @ Fri, 26 Jun 2020 07:31:43: 18000000 INFO @ Fri, 26 Jun 2020 07:31:47: 23000000 INFO @ Fri, 26 Jun 2020 07:31:50: 29000000 INFO @ Fri, 26 Jun 2020 07:31:51: 19000000 INFO @ Fri, 26 Jun 2020 07:31:55: 24000000 INFO @ Fri, 26 Jun 2020 07:31:57: 30000000 INFO @ Fri, 26 Jun 2020 07:31:57: 20000000 INFO @ Fri, 26 Jun 2020 07:32:02: 25000000 INFO @ Fri, 26 Jun 2020 07:32:04: 31000000 INFO @ Fri, 26 Jun 2020 07:32:04: 21000000 INFO @ Fri, 26 Jun 2020 07:32:09: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 07:32:12: 32000000 INFO @ Fri, 26 Jun 2020 07:32:12: 22000000 INFO @ Fri, 26 Jun 2020 07:32:16: 27000000 INFO @ Fri, 26 Jun 2020 07:32:19: 23000000 INFO @ Fri, 26 Jun 2020 07:32:19: 33000000 INFO @ Fri, 26 Jun 2020 07:32:24: 28000000 INFO @ Fri, 26 Jun 2020 07:32:26: 24000000 INFO @ Fri, 26 Jun 2020 07:32:26: 34000000 INFO @ Fri, 26 Jun 2020 07:32:31: 29000000 INFO @ Fri, 26 Jun 2020 07:32:33: 25000000 INFO @ Fri, 26 Jun 2020 07:32:33: 35000000 INFO @ Fri, 26 Jun 2020 07:32:38: 30000000 INFO @ Fri, 26 Jun 2020 07:32:40: 26000000 INFO @ Fri, 26 Jun 2020 07:32:40: 36000000 INFO @ Fri, 26 Jun 2020 07:32:45: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:32:45: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:32:45: #1 total tags in treatment: 36623303 INFO @ Fri, 26 Jun 2020 07:32:45: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:32:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:32:46: 31000000 INFO @ Fri, 26 Jun 2020 07:32:46: #1 tags after filtering in treatment: 36623303 INFO @ Fri, 26 Jun 2020 07:32:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:32:46: #1 finished! INFO @ Fri, 26 Jun 2020 07:32:46: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:32:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:32:47: 27000000 INFO @ Fri, 26 Jun 2020 07:32:48: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 07:32:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 07:32:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:32:53: 32000000 INFO @ Fri, 26 Jun 2020 07:32:53: 28000000 INFO @ Fri, 26 Jun 2020 07:33:00: 33000000 INFO @ Fri, 26 Jun 2020 07:33:00: 29000000 INFO @ Fri, 26 Jun 2020 07:33:07: 34000000 INFO @ Fri, 26 Jun 2020 07:33:07: 30000000 INFO @ Fri, 26 Jun 2020 07:33:14: 31000000 INFO @ Fri, 26 Jun 2020 07:33:15: 35000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 07:33:22: 32000000 INFO @ Fri, 26 Jun 2020 07:33:22: 36000000 INFO @ Fri, 26 Jun 2020 07:33:27: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:33:27: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:33:27: #1 total tags in treatment: 36623303 INFO @ Fri, 26 Jun 2020 07:33:27: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:33:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:33:27: #1 tags after filtering in treatment: 36623303 INFO @ Fri, 26 Jun 2020 07:33:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:33:27: #1 finished! INFO @ Fri, 26 Jun 2020 07:33:27: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:33:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:33:29: 33000000 INFO @ Fri, 26 Jun 2020 07:33:30: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 07:33:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 07:33:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:33:35: 34000000 INFO @ Fri, 26 Jun 2020 07:33:41: 35000000 INFO @ Fri, 26 Jun 2020 07:33:48: 36000000 INFO @ Fri, 26 Jun 2020 07:33:52: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:33:52: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:33:52: #1 total tags in treatment: 36623303 INFO @ Fri, 26 Jun 2020 07:33:52: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:33:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:33:53: #1 tags after filtering in treatment: 36623303 INFO @ Fri, 26 Jun 2020 07:33:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:33:53: #1 finished! INFO @ Fri, 26 Jun 2020 07:33:53: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:33:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:33:55: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 07:33:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 07:33:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494928/SRX494928.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling