Job ID = 2590031 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:16 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139827 (6.55%) aligned 0 times 37484916 (78.17%) aligned exactly 1 time 7325872 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:16 Overall time: 00:12:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188952 / 44810788 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:59:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:59:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:59:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:59:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:59:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:59:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:59:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:59:45: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:59:45: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:59:53: 1000000 INFO @ Mon, 12 Aug 2019 19:59:53: 1000000 INFO @ Mon, 12 Aug 2019 19:59:54: 1000000 INFO @ Mon, 12 Aug 2019 20:00:01: 2000000 INFO @ Mon, 12 Aug 2019 20:00:02: 2000000 INFO @ Mon, 12 Aug 2019 20:00:03: 2000000 INFO @ Mon, 12 Aug 2019 20:00:09: 3000000 INFO @ Mon, 12 Aug 2019 20:00:10: 3000000 INFO @ Mon, 12 Aug 2019 20:00:12: 3000000 INFO @ Mon, 12 Aug 2019 20:00:17: 4000000 INFO @ Mon, 12 Aug 2019 20:00:18: 4000000 INFO @ Mon, 12 Aug 2019 20:00:22: 4000000 INFO @ Mon, 12 Aug 2019 20:00:25: 5000000 INFO @ Mon, 12 Aug 2019 20:00:25: 5000000 INFO @ Mon, 12 Aug 2019 20:00:31: 5000000 INFO @ Mon, 12 Aug 2019 20:00:32: 6000000 INFO @ Mon, 12 Aug 2019 20:00:33: 6000000 INFO @ Mon, 12 Aug 2019 20:00:40: 7000000 INFO @ Mon, 12 Aug 2019 20:00:40: 6000000 INFO @ Mon, 12 Aug 2019 20:00:41: 7000000 INFO @ Mon, 12 Aug 2019 20:00:47: 8000000 INFO @ Mon, 12 Aug 2019 20:00:48: 8000000 INFO @ Mon, 12 Aug 2019 20:00:49: 7000000 INFO @ Mon, 12 Aug 2019 20:00:54: 9000000 INFO @ Mon, 12 Aug 2019 20:00:56: 9000000 INFO @ Mon, 12 Aug 2019 20:00:58: 8000000 INFO @ Mon, 12 Aug 2019 20:01:02: 10000000 INFO @ Mon, 12 Aug 2019 20:01:06: 10000000 INFO @ Mon, 12 Aug 2019 20:01:08: 9000000 INFO @ Mon, 12 Aug 2019 20:01:09: 11000000 INFO @ Mon, 12 Aug 2019 20:01:14: 11000000 INFO @ Mon, 12 Aug 2019 20:01:16: 12000000 INFO @ Mon, 12 Aug 2019 20:01:17: 10000000 INFO @ Mon, 12 Aug 2019 20:01:21: 12000000 INFO @ Mon, 12 Aug 2019 20:01:23: 13000000 INFO @ Mon, 12 Aug 2019 20:01:26: 11000000 INFO @ Mon, 12 Aug 2019 20:01:29: 13000000 INFO @ Mon, 12 Aug 2019 20:01:30: 14000000 INFO @ Mon, 12 Aug 2019 20:01:35: 12000000 INFO @ Mon, 12 Aug 2019 20:01:37: 14000000 INFO @ Mon, 12 Aug 2019 20:01:37: 15000000 INFO @ Mon, 12 Aug 2019 20:01:44: 13000000 INFO @ Mon, 12 Aug 2019 20:01:44: 15000000 INFO @ Mon, 12 Aug 2019 20:01:44: 16000000 INFO @ Mon, 12 Aug 2019 20:01:52: 17000000 INFO @ Mon, 12 Aug 2019 20:01:52: 16000000 INFO @ Mon, 12 Aug 2019 20:01:53: 14000000 INFO @ Mon, 12 Aug 2019 20:01:59: 18000000 INFO @ Mon, 12 Aug 2019 20:02:00: 17000000 INFO @ Mon, 12 Aug 2019 20:02:02: 15000000 INFO @ Mon, 12 Aug 2019 20:02:06: 19000000 INFO @ Mon, 12 Aug 2019 20:02:07: 18000000 INFO @ Mon, 12 Aug 2019 20:02:11: 16000000 INFO @ Mon, 12 Aug 2019 20:02:13: 20000000 INFO @ Mon, 12 Aug 2019 20:02:15: 19000000 INFO @ Mon, 12 Aug 2019 20:02:20: 17000000 INFO @ Mon, 12 Aug 2019 20:02:20: 21000000 INFO @ Mon, 12 Aug 2019 20:02:23: 20000000 INFO @ Mon, 12 Aug 2019 20:02:27: 22000000 INFO @ Mon, 12 Aug 2019 20:02:29: 18000000 INFO @ Mon, 12 Aug 2019 20:02:31: 21000000 INFO @ Mon, 12 Aug 2019 20:02:35: 23000000 INFO @ Mon, 12 Aug 2019 20:02:37: 19000000 INFO @ Mon, 12 Aug 2019 20:02:38: 22000000 INFO @ Mon, 12 Aug 2019 20:02:42: 24000000 INFO @ Mon, 12 Aug 2019 20:02:45: 20000000 INFO @ Mon, 12 Aug 2019 20:02:46: 23000000 INFO @ Mon, 12 Aug 2019 20:02:49: 25000000 INFO @ Mon, 12 Aug 2019 20:02:52: 21000000 INFO @ Mon, 12 Aug 2019 20:02:54: 24000000 INFO @ Mon, 12 Aug 2019 20:02:56: 26000000 INFO @ Mon, 12 Aug 2019 20:02:59: 22000000 INFO @ Mon, 12 Aug 2019 20:03:02: 25000000 INFO @ Mon, 12 Aug 2019 20:03:03: 27000000 INFO @ Mon, 12 Aug 2019 20:03:07: 23000000 INFO @ Mon, 12 Aug 2019 20:03:10: 26000000 INFO @ Mon, 12 Aug 2019 20:03:11: 28000000 INFO @ Mon, 12 Aug 2019 20:03:15: 24000000 INFO @ Mon, 12 Aug 2019 20:03:18: 27000000 INFO @ Mon, 12 Aug 2019 20:03:19: 29000000 INFO @ Mon, 12 Aug 2019 20:03:24: 25000000 INFO @ Mon, 12 Aug 2019 20:03:26: 28000000 INFO @ Mon, 12 Aug 2019 20:03:27: 30000000 INFO @ Mon, 12 Aug 2019 20:03:32: 26000000 INFO @ Mon, 12 Aug 2019 20:03:35: 29000000 INFO @ Mon, 12 Aug 2019 20:03:35: 31000000 INFO @ Mon, 12 Aug 2019 20:03:40: 27000000 INFO @ Mon, 12 Aug 2019 20:03:42: 32000000 INFO @ Mon, 12 Aug 2019 20:03:43: 30000000 INFO @ Mon, 12 Aug 2019 20:03:47: 28000000 INFO @ Mon, 12 Aug 2019 20:03:51: 33000000 INFO @ Mon, 12 Aug 2019 20:03:52: 31000000 INFO @ Mon, 12 Aug 2019 20:03:55: 29000000 INFO @ Mon, 12 Aug 2019 20:03:58: 34000000 INFO @ Mon, 12 Aug 2019 20:04:00: 32000000 INFO @ Mon, 12 Aug 2019 20:04:02: 30000000 INFO @ Mon, 12 Aug 2019 20:04:06: 35000000 INFO @ Mon, 12 Aug 2019 20:04:08: 33000000 INFO @ Mon, 12 Aug 2019 20:04:10: 31000000 INFO @ Mon, 12 Aug 2019 20:04:15: 36000000 INFO @ Mon, 12 Aug 2019 20:04:16: 34000000 INFO @ Mon, 12 Aug 2019 20:04:17: 32000000 INFO @ Mon, 12 Aug 2019 20:04:20: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:04:20: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:04:20: #1 total tags in treatment: 36621836 INFO @ Mon, 12 Aug 2019 20:04:20: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:04:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:04:21: #1 tags after filtering in treatment: 36621836 INFO @ Mon, 12 Aug 2019 20:04:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:04:21: #1 finished! INFO @ Mon, 12 Aug 2019 20:04:21: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:04:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:04:24: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:04:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:04:24: Process for pairing-model is terminated! INFO @ Mon, 12 Aug 2019 20:04:24: 35000000 cut: /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:04:25: 33000000 INFO @ Mon, 12 Aug 2019 20:04:32: 34000000 INFO @ Mon, 12 Aug 2019 20:04:33: 36000000 INFO @ Mon, 12 Aug 2019 20:04:38: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:04:38: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:04:38: #1 total tags in treatment: 36621836 INFO @ Mon, 12 Aug 2019 20:04:38: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:04:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:04:39: #1 tags after filtering in treatment: 36621836 INFO @ Mon, 12 Aug 2019 20:04:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:04:39: #1 finished! INFO @ Mon, 12 Aug 2019 20:04:39: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:04:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:04:39: 35000000 INFO @ Mon, 12 Aug 2019 20:04:42: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:04:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:04:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:04:47: 36000000 INFO @ Mon, 12 Aug 2019 20:04:52: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:04:52: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:04:52: #1 total tags in treatment: 36621836 INFO @ Mon, 12 Aug 2019 20:04:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:04:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:04:52: #1 tags after filtering in treatment: 36621836 INFO @ Mon, 12 Aug 2019 20:04:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:04:52: #1 finished! INFO @ Mon, 12 Aug 2019 20:04:52: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:04:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:04:55: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:04:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:04:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494875/SRX494875.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。