Job ID = 2590021 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198397.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:44 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139837 (6.55%) aligned 0 times 37484936 (78.17%) aligned exactly 1 time 7325842 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:44 Overall time: 00:12:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188836 / 44810778 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:34:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:34:08: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:34:08: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:34:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:34:09: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:34:09: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:34:10: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:34:10: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:34:18: 1000000 INFO @ Mon, 12 Aug 2019 19:34:19: 1000000 INFO @ Mon, 12 Aug 2019 19:34:20: 1000000 INFO @ Mon, 12 Aug 2019 19:34:25: 2000000 INFO @ Mon, 12 Aug 2019 19:34:29: 2000000 INFO @ Mon, 12 Aug 2019 19:34:29: 2000000 INFO @ Mon, 12 Aug 2019 19:34:32: 3000000 INFO @ Mon, 12 Aug 2019 19:34:38: 3000000 INFO @ Mon, 12 Aug 2019 19:34:39: 4000000 INFO @ Mon, 12 Aug 2019 19:34:39: 3000000 INFO @ Mon, 12 Aug 2019 19:34:46: 5000000 INFO @ Mon, 12 Aug 2019 19:34:48: 4000000 INFO @ Mon, 12 Aug 2019 19:34:49: 4000000 INFO @ Mon, 12 Aug 2019 19:34:54: 6000000 INFO @ Mon, 12 Aug 2019 19:34:58: 5000000 INFO @ Mon, 12 Aug 2019 19:34:59: 5000000 INFO @ Mon, 12 Aug 2019 19:35:01: 7000000 INFO @ Mon, 12 Aug 2019 19:35:07: 6000000 INFO @ Mon, 12 Aug 2019 19:35:09: 8000000 INFO @ Mon, 12 Aug 2019 19:35:09: 6000000 INFO @ Mon, 12 Aug 2019 19:35:16: 9000000 INFO @ Mon, 12 Aug 2019 19:35:17: 7000000 INFO @ Mon, 12 Aug 2019 19:35:20: 7000000 INFO @ Mon, 12 Aug 2019 19:35:23: 10000000 INFO @ Mon, 12 Aug 2019 19:35:27: 8000000 INFO @ Mon, 12 Aug 2019 19:35:29: 8000000 INFO @ Mon, 12 Aug 2019 19:35:30: 11000000 INFO @ Mon, 12 Aug 2019 19:35:37: 9000000 INFO @ Mon, 12 Aug 2019 19:35:37: 12000000 INFO @ Mon, 12 Aug 2019 19:35:39: 9000000 INFO @ Mon, 12 Aug 2019 19:35:44: 13000000 INFO @ Mon, 12 Aug 2019 19:35:46: 10000000 INFO @ Mon, 12 Aug 2019 19:35:49: 10000000 INFO @ Mon, 12 Aug 2019 19:35:51: 14000000 INFO @ Mon, 12 Aug 2019 19:35:56: 11000000 INFO @ Mon, 12 Aug 2019 19:35:58: 11000000 INFO @ Mon, 12 Aug 2019 19:35:58: 15000000 INFO @ Mon, 12 Aug 2019 19:36:05: 12000000 INFO @ Mon, 12 Aug 2019 19:36:05: 16000000 INFO @ Mon, 12 Aug 2019 19:36:08: 12000000 INFO @ Mon, 12 Aug 2019 19:36:12: 17000000 INFO @ Mon, 12 Aug 2019 19:36:14: 13000000 INFO @ Mon, 12 Aug 2019 19:36:17: 13000000 INFO @ Mon, 12 Aug 2019 19:36:19: 18000000 INFO @ Mon, 12 Aug 2019 19:36:23: 14000000 INFO @ Mon, 12 Aug 2019 19:36:25: 14000000 INFO @ Mon, 12 Aug 2019 19:36:26: 19000000 INFO @ Mon, 12 Aug 2019 19:36:31: 15000000 INFO @ Mon, 12 Aug 2019 19:36:33: 20000000 INFO @ Mon, 12 Aug 2019 19:36:34: 15000000 INFO @ Mon, 12 Aug 2019 19:36:39: 16000000 INFO @ Mon, 12 Aug 2019 19:36:40: 21000000 INFO @ Mon, 12 Aug 2019 19:36:43: 16000000 INFO @ Mon, 12 Aug 2019 19:36:47: 22000000 INFO @ Mon, 12 Aug 2019 19:36:48: 17000000 INFO @ Mon, 12 Aug 2019 19:36:52: 17000000 INFO @ Mon, 12 Aug 2019 19:36:54: 23000000 INFO @ Mon, 12 Aug 2019 19:36:57: 18000000 INFO @ Mon, 12 Aug 2019 19:37:01: 18000000 INFO @ Mon, 12 Aug 2019 19:37:01: 24000000 INFO @ Mon, 12 Aug 2019 19:37:06: 19000000 INFO @ Mon, 12 Aug 2019 19:37:08: 25000000 INFO @ Mon, 12 Aug 2019 19:37:10: 19000000 INFO @ Mon, 12 Aug 2019 19:37:15: 26000000 INFO @ Mon, 12 Aug 2019 19:37:15: 20000000 INFO @ Mon, 12 Aug 2019 19:37:20: 20000000 INFO @ Mon, 12 Aug 2019 19:37:22: 27000000 INFO @ Mon, 12 Aug 2019 19:37:25: 21000000 INFO @ Mon, 12 Aug 2019 19:37:29: 28000000 INFO @ Mon, 12 Aug 2019 19:37:29: 21000000 INFO @ Mon, 12 Aug 2019 19:37:35: 22000000 INFO @ Mon, 12 Aug 2019 19:37:36: 29000000 INFO @ Mon, 12 Aug 2019 19:37:39: 22000000 INFO @ Mon, 12 Aug 2019 19:37:43: 30000000 INFO @ Mon, 12 Aug 2019 19:37:44: 23000000 INFO @ Mon, 12 Aug 2019 19:37:48: 23000000 INFO @ Mon, 12 Aug 2019 19:37:50: 31000000 INFO @ Mon, 12 Aug 2019 19:37:54: 24000000 INFO @ Mon, 12 Aug 2019 19:37:57: 32000000 INFO @ Mon, 12 Aug 2019 19:37:58: 24000000 INFO @ Mon, 12 Aug 2019 19:38:04: 25000000 INFO @ Mon, 12 Aug 2019 19:38:04: 33000000 INFO @ Mon, 12 Aug 2019 19:38:08: 25000000 INFO @ Mon, 12 Aug 2019 19:38:11: 34000000 INFO @ Mon, 12 Aug 2019 19:38:13: 26000000 INFO @ Mon, 12 Aug 2019 19:38:17: 26000000 INFO @ Mon, 12 Aug 2019 19:38:17: 35000000 INFO @ Mon, 12 Aug 2019 19:38:23: 27000000 INFO @ Mon, 12 Aug 2019 19:38:24: 36000000 INFO @ Mon, 12 Aug 2019 19:38:27: 27000000 INFO @ Mon, 12 Aug 2019 19:38:29: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:38:29: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:38:29: #1 total tags in treatment: 36621942 INFO @ Mon, 12 Aug 2019 19:38:29: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:38:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:38:30: #1 tags after filtering in treatment: 36621942 INFO @ Mon, 12 Aug 2019 19:38:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:38:30: #1 finished! INFO @ Mon, 12 Aug 2019 19:38:30: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:38:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:38:33: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:38:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:38:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:38:33: 28000000 INFO @ Mon, 12 Aug 2019 19:38:37: 28000000 INFO @ Mon, 12 Aug 2019 19:38:42: 29000000 INFO @ Mon, 12 Aug 2019 19:38:46: 29000000 INFO @ Mon, 12 Aug 2019 19:38:52: 30000000 INFO @ Mon, 12 Aug 2019 19:38:56: 30000000 INFO @ Mon, 12 Aug 2019 19:39:01: 31000000 INFO @ Mon, 12 Aug 2019 19:39:05: 31000000 INFO @ Mon, 12 Aug 2019 19:39:11: 32000000 INFO @ Mon, 12 Aug 2019 19:39:15: 32000000 INFO @ Mon, 12 Aug 2019 19:39:21: 33000000 INFO @ Mon, 12 Aug 2019 19:39:25: 33000000 INFO @ Mon, 12 Aug 2019 19:39:30: 34000000 INFO @ Mon, 12 Aug 2019 19:39:34: 34000000 INFO @ Mon, 12 Aug 2019 19:39:39: 35000000 INFO @ Mon, 12 Aug 2019 19:39:43: 35000000 INFO @ Mon, 12 Aug 2019 19:39:49: 36000000 INFO @ Mon, 12 Aug 2019 19:39:53: 36000000 INFO @ Mon, 12 Aug 2019 19:39:55: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:39:55: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:39:55: #1 total tags in treatment: 36621942 INFO @ Mon, 12 Aug 2019 19:39:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:39:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:39:56: #1 tags after filtering in treatment: 36621942 INFO @ Mon, 12 Aug 2019 19:39:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:39:56: #1 finished! INFO @ Mon, 12 Aug 2019 19:39:56: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:39:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:39:58: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:39:58: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:39:58: #1 total tags in treatment: 36621942 INFO @ Mon, 12 Aug 2019 19:39:58: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:39:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:39:59: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:39:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:39:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:39:59: #1 tags after filtering in treatment: 36621942 INFO @ Mon, 12 Aug 2019 19:39:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:39:59: #1 finished! INFO @ Mon, 12 Aug 2019 19:39:59: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:39:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:40:02: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:40:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:40:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494865/SRX494865.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。