Job ID = 2589983 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 51,067,685 reads read : 51,067,685 reads written : 51,067,685 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1163635.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:20 51067685 reads; of these: 51067685 (100.00%) were unpaired; of these: 4586378 (8.98%) aligned 0 times 37934060 (74.28%) aligned exactly 1 time 8547247 (16.74%) aligned >1 times 91.02% overall alignment rate Time searching: 00:12:20 Overall time: 00:12:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9745319 / 46481307 = 0.2097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:24:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:24:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:24:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:24:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:24:25: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:24:25: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:24:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:24:26: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:24:26: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:24:35: 1000000 INFO @ Mon, 12 Aug 2019 19:24:35: 1000000 INFO @ Mon, 12 Aug 2019 19:24:36: 1000000 INFO @ Mon, 12 Aug 2019 19:24:44: 2000000 INFO @ Mon, 12 Aug 2019 19:24:45: 2000000 INFO @ Mon, 12 Aug 2019 19:24:45: 2000000 INFO @ Mon, 12 Aug 2019 19:24:54: 3000000 INFO @ Mon, 12 Aug 2019 19:24:55: 3000000 INFO @ Mon, 12 Aug 2019 19:24:56: 3000000 INFO @ Mon, 12 Aug 2019 19:25:03: 4000000 INFO @ Mon, 12 Aug 2019 19:25:04: 4000000 INFO @ Mon, 12 Aug 2019 19:25:06: 4000000 INFO @ Mon, 12 Aug 2019 19:25:13: 5000000 INFO @ Mon, 12 Aug 2019 19:25:14: 5000000 INFO @ Mon, 12 Aug 2019 19:25:16: 5000000 INFO @ Mon, 12 Aug 2019 19:25:22: 6000000 INFO @ Mon, 12 Aug 2019 19:25:23: 6000000 INFO @ Mon, 12 Aug 2019 19:25:25: 6000000 INFO @ Mon, 12 Aug 2019 19:25:32: 7000000 INFO @ Mon, 12 Aug 2019 19:25:33: 7000000 INFO @ Mon, 12 Aug 2019 19:25:35: 7000000 INFO @ Mon, 12 Aug 2019 19:25:41: 8000000 INFO @ Mon, 12 Aug 2019 19:25:42: 8000000 INFO @ Mon, 12 Aug 2019 19:25:45: 8000000 INFO @ Mon, 12 Aug 2019 19:25:51: 9000000 INFO @ Mon, 12 Aug 2019 19:25:52: 9000000 INFO @ Mon, 12 Aug 2019 19:25:55: 9000000 INFO @ Mon, 12 Aug 2019 19:26:00: 10000000 INFO @ Mon, 12 Aug 2019 19:26:01: 10000000 INFO @ Mon, 12 Aug 2019 19:26:05: 10000000 INFO @ Mon, 12 Aug 2019 19:26:10: 11000000 INFO @ Mon, 12 Aug 2019 19:26:11: 11000000 INFO @ Mon, 12 Aug 2019 19:26:15: 11000000 INFO @ Mon, 12 Aug 2019 19:26:19: 12000000 INFO @ Mon, 12 Aug 2019 19:26:20: 12000000 INFO @ Mon, 12 Aug 2019 19:26:24: 12000000 INFO @ Mon, 12 Aug 2019 19:26:29: 13000000 INFO @ Mon, 12 Aug 2019 19:26:30: 13000000 INFO @ Mon, 12 Aug 2019 19:26:34: 13000000 INFO @ Mon, 12 Aug 2019 19:26:38: 14000000 INFO @ Mon, 12 Aug 2019 19:26:39: 14000000 INFO @ Mon, 12 Aug 2019 19:26:43: 14000000 INFO @ Mon, 12 Aug 2019 19:26:47: 15000000 INFO @ Mon, 12 Aug 2019 19:26:48: 15000000 INFO @ Mon, 12 Aug 2019 19:26:51: 15000000 INFO @ Mon, 12 Aug 2019 19:26:56: 16000000 INFO @ Mon, 12 Aug 2019 19:26:57: 16000000 INFO @ Mon, 12 Aug 2019 19:26:59: 16000000 INFO @ Mon, 12 Aug 2019 19:27:06: 17000000 INFO @ Mon, 12 Aug 2019 19:27:07: 17000000 INFO @ Mon, 12 Aug 2019 19:27:08: 17000000 INFO @ Mon, 12 Aug 2019 19:27:15: 18000000 INFO @ Mon, 12 Aug 2019 19:27:16: 18000000 INFO @ Mon, 12 Aug 2019 19:27:17: 18000000 INFO @ Mon, 12 Aug 2019 19:27:24: 19000000 INFO @ Mon, 12 Aug 2019 19:27:25: 19000000 INFO @ Mon, 12 Aug 2019 19:27:25: 19000000 INFO @ Mon, 12 Aug 2019 19:27:33: 20000000 INFO @ Mon, 12 Aug 2019 19:27:33: 20000000 INFO @ Mon, 12 Aug 2019 19:27:34: 20000000 INFO @ Mon, 12 Aug 2019 19:27:41: 21000000 INFO @ Mon, 12 Aug 2019 19:27:42: 21000000 INFO @ Mon, 12 Aug 2019 19:27:44: 21000000 INFO @ Mon, 12 Aug 2019 19:27:49: 22000000 INFO @ Mon, 12 Aug 2019 19:27:52: 22000000 INFO @ Mon, 12 Aug 2019 19:27:53: 22000000 INFO @ Mon, 12 Aug 2019 19:27:58: 23000000 INFO @ Mon, 12 Aug 2019 19:28:01: 23000000 INFO @ Mon, 12 Aug 2019 19:28:02: 23000000 INFO @ Mon, 12 Aug 2019 19:28:06: 24000000 INFO @ Mon, 12 Aug 2019 19:28:10: 24000000 INFO @ Mon, 12 Aug 2019 19:28:11: 24000000 INFO @ Mon, 12 Aug 2019 19:28:14: 25000000 INFO @ Mon, 12 Aug 2019 19:28:19: 25000000 INFO @ Mon, 12 Aug 2019 19:28:20: 25000000 INFO @ Mon, 12 Aug 2019 19:28:22: 26000000 INFO @ Mon, 12 Aug 2019 19:28:28: 26000000 INFO @ Mon, 12 Aug 2019 19:28:29: 26000000 INFO @ Mon, 12 Aug 2019 19:28:30: 27000000 INFO @ Mon, 12 Aug 2019 19:28:37: 27000000 INFO @ Mon, 12 Aug 2019 19:28:38: 28000000 INFO @ Mon, 12 Aug 2019 19:28:38: 27000000 INFO @ Mon, 12 Aug 2019 19:28:46: 29000000 INFO @ Mon, 12 Aug 2019 19:28:46: 28000000 INFO @ Mon, 12 Aug 2019 19:28:48: 28000000 INFO @ Mon, 12 Aug 2019 19:28:54: 30000000 INFO @ Mon, 12 Aug 2019 19:28:55: 29000000 INFO @ Mon, 12 Aug 2019 19:28:56: 29000000 INFO @ Mon, 12 Aug 2019 19:29:02: 31000000 INFO @ Mon, 12 Aug 2019 19:29:04: 30000000 INFO @ Mon, 12 Aug 2019 19:29:05: 30000000 INFO @ Mon, 12 Aug 2019 19:29:09: 32000000 INFO @ Mon, 12 Aug 2019 19:29:13: 31000000 INFO @ Mon, 12 Aug 2019 19:29:14: 31000000 INFO @ Mon, 12 Aug 2019 19:29:17: 33000000 INFO @ Mon, 12 Aug 2019 19:29:21: 32000000 INFO @ Mon, 12 Aug 2019 19:29:23: 32000000 INFO @ Mon, 12 Aug 2019 19:29:25: 34000000 INFO @ Mon, 12 Aug 2019 19:29:30: 33000000 INFO @ Mon, 12 Aug 2019 19:29:31: 33000000 INFO @ Mon, 12 Aug 2019 19:29:33: 35000000 INFO @ Mon, 12 Aug 2019 19:29:39: 34000000 INFO @ Mon, 12 Aug 2019 19:29:40: 34000000 INFO @ Mon, 12 Aug 2019 19:29:40: 36000000 INFO @ Mon, 12 Aug 2019 19:29:47: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:29:47: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:29:47: #1 total tags in treatment: 36735988 INFO @ Mon, 12 Aug 2019 19:29:47: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:29:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:29:47: #1 tags after filtering in treatment: 36735988 INFO @ Mon, 12 Aug 2019 19:29:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:29:47: #1 finished! INFO @ Mon, 12 Aug 2019 19:29:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:29:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:29:47: 35000000 INFO @ Mon, 12 Aug 2019 19:29:49: 35000000 INFO @ Mon, 12 Aug 2019 19:29:50: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:29:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:29:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:29:56: 36000000 INFO @ Mon, 12 Aug 2019 19:29:57: 36000000 INFO @ Mon, 12 Aug 2019 19:30:03: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:30:03: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:30:03: #1 total tags in treatment: 36735988 INFO @ Mon, 12 Aug 2019 19:30:03: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:30:03: #1 tags after filtering in treatment: 36735988 INFO @ Mon, 12 Aug 2019 19:30:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:30:03: #1 finished! INFO @ Mon, 12 Aug 2019 19:30:03: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:30:04: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:30:04: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:30:04: #1 total tags in treatment: 36735988 INFO @ Mon, 12 Aug 2019 19:30:04: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:30:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:30:04: #1 tags after filtering in treatment: 36735988 INFO @ Mon, 12 Aug 2019 19:30:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:30:04: #1 finished! INFO @ Mon, 12 Aug 2019 19:30:04: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:30:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:30:06: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:30:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:30:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:30:07: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:30:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:30:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466569/SRX466569.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。