Job ID = 2589978 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1163630.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:07 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139827 (6.55%) aligned 0 times 37485065 (78.17%) aligned exactly 1 time 7325723 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:11:07 Overall time: 00:11:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188821 / 44810788 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:21:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:21:07: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:21:07: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:21:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:21:08: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:21:08: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:21:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:21:09: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:21:09: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:21:18: 1000000 INFO @ Mon, 12 Aug 2019 19:21:18: 1000000 INFO @ Mon, 12 Aug 2019 19:21:21: 1000000 INFO @ Mon, 12 Aug 2019 19:21:27: 2000000 INFO @ Mon, 12 Aug 2019 19:21:30: 2000000 INFO @ Mon, 12 Aug 2019 19:21:32: 2000000 INFO @ Mon, 12 Aug 2019 19:21:37: 3000000 INFO @ Mon, 12 Aug 2019 19:21:41: 3000000 INFO @ Mon, 12 Aug 2019 19:21:43: 3000000 INFO @ Mon, 12 Aug 2019 19:21:46: 4000000 INFO @ Mon, 12 Aug 2019 19:21:52: 4000000 INFO @ Mon, 12 Aug 2019 19:21:54: 4000000 INFO @ Mon, 12 Aug 2019 19:21:55: 5000000 INFO @ Mon, 12 Aug 2019 19:22:03: 5000000 INFO @ Mon, 12 Aug 2019 19:22:04: 6000000 INFO @ Mon, 12 Aug 2019 19:22:05: 5000000 INFO @ Mon, 12 Aug 2019 19:22:14: 6000000 INFO @ Mon, 12 Aug 2019 19:22:14: 7000000 INFO @ Mon, 12 Aug 2019 19:22:16: 6000000 INFO @ Mon, 12 Aug 2019 19:22:24: 8000000 INFO @ Mon, 12 Aug 2019 19:22:24: 7000000 INFO @ Mon, 12 Aug 2019 19:22:26: 7000000 INFO @ Mon, 12 Aug 2019 19:22:33: 9000000 INFO @ Mon, 12 Aug 2019 19:22:35: 8000000 INFO @ Mon, 12 Aug 2019 19:22:37: 8000000 INFO @ Mon, 12 Aug 2019 19:22:42: 10000000 INFO @ Mon, 12 Aug 2019 19:22:46: 9000000 INFO @ Mon, 12 Aug 2019 19:22:48: 9000000 INFO @ Mon, 12 Aug 2019 19:22:52: 11000000 INFO @ Mon, 12 Aug 2019 19:22:56: 10000000 INFO @ Mon, 12 Aug 2019 19:22:58: 10000000 INFO @ Mon, 12 Aug 2019 19:23:02: 12000000 INFO @ Mon, 12 Aug 2019 19:23:07: 11000000 INFO @ Mon, 12 Aug 2019 19:23:09: 11000000 INFO @ Mon, 12 Aug 2019 19:23:11: 13000000 INFO @ Mon, 12 Aug 2019 19:23:17: 12000000 INFO @ Mon, 12 Aug 2019 19:23:19: 12000000 INFO @ Mon, 12 Aug 2019 19:23:20: 14000000 INFO @ Mon, 12 Aug 2019 19:23:28: 13000000 INFO @ Mon, 12 Aug 2019 19:23:30: 13000000 INFO @ Mon, 12 Aug 2019 19:23:30: 15000000 INFO @ Mon, 12 Aug 2019 19:23:38: 14000000 INFO @ Mon, 12 Aug 2019 19:23:39: 16000000 INFO @ Mon, 12 Aug 2019 19:23:40: 14000000 INFO @ Mon, 12 Aug 2019 19:23:49: 15000000 INFO @ Mon, 12 Aug 2019 19:23:49: 17000000 INFO @ Mon, 12 Aug 2019 19:23:50: 15000000 INFO @ Mon, 12 Aug 2019 19:23:58: 18000000 INFO @ Mon, 12 Aug 2019 19:23:59: 16000000 INFO @ Mon, 12 Aug 2019 19:24:01: 16000000 INFO @ Mon, 12 Aug 2019 19:24:07: 19000000 INFO @ Mon, 12 Aug 2019 19:24:09: 17000000 INFO @ Mon, 12 Aug 2019 19:24:11: 17000000 INFO @ Mon, 12 Aug 2019 19:24:17: 20000000 INFO @ Mon, 12 Aug 2019 19:24:20: 18000000 INFO @ Mon, 12 Aug 2019 19:24:22: 18000000 INFO @ Mon, 12 Aug 2019 19:24:26: 21000000 INFO @ Mon, 12 Aug 2019 19:24:30: 19000000 INFO @ Mon, 12 Aug 2019 19:24:32: 19000000 INFO @ Mon, 12 Aug 2019 19:24:35: 22000000 INFO @ Mon, 12 Aug 2019 19:24:41: 20000000 INFO @ Mon, 12 Aug 2019 19:24:42: 20000000 INFO @ Mon, 12 Aug 2019 19:24:45: 23000000 INFO @ Mon, 12 Aug 2019 19:24:51: 21000000 INFO @ Mon, 12 Aug 2019 19:24:53: 21000000 INFO @ Mon, 12 Aug 2019 19:24:54: 24000000 INFO @ Mon, 12 Aug 2019 19:25:01: 22000000 INFO @ Mon, 12 Aug 2019 19:25:03: 22000000 INFO @ Mon, 12 Aug 2019 19:25:03: 25000000 INFO @ Mon, 12 Aug 2019 19:25:12: 23000000 INFO @ Mon, 12 Aug 2019 19:25:12: 26000000 INFO @ Mon, 12 Aug 2019 19:25:13: 23000000 INFO @ Mon, 12 Aug 2019 19:25:22: 27000000 INFO @ Mon, 12 Aug 2019 19:25:22: 24000000 INFO @ Mon, 12 Aug 2019 19:25:24: 24000000 INFO @ Mon, 12 Aug 2019 19:25:31: 28000000 INFO @ Mon, 12 Aug 2019 19:25:33: 25000000 INFO @ Mon, 12 Aug 2019 19:25:34: 25000000 INFO @ Mon, 12 Aug 2019 19:25:40: 29000000 INFO @ Mon, 12 Aug 2019 19:25:43: 26000000 INFO @ Mon, 12 Aug 2019 19:25:44: 26000000 INFO @ Mon, 12 Aug 2019 19:25:49: 30000000 INFO @ Mon, 12 Aug 2019 19:25:53: 27000000 INFO @ Mon, 12 Aug 2019 19:25:55: 27000000 INFO @ Mon, 12 Aug 2019 19:25:59: 31000000 INFO @ Mon, 12 Aug 2019 19:26:04: 28000000 INFO @ Mon, 12 Aug 2019 19:26:05: 28000000 INFO @ Mon, 12 Aug 2019 19:26:08: 32000000 INFO @ Mon, 12 Aug 2019 19:26:13: 29000000 INFO @ Mon, 12 Aug 2019 19:26:15: 29000000 INFO @ Mon, 12 Aug 2019 19:26:17: 33000000 INFO @ Mon, 12 Aug 2019 19:26:24: 30000000 INFO @ Mon, 12 Aug 2019 19:26:25: 30000000 INFO @ Mon, 12 Aug 2019 19:26:26: 34000000 INFO @ Mon, 12 Aug 2019 19:26:34: 31000000 INFO @ Mon, 12 Aug 2019 19:26:35: 35000000 INFO @ Mon, 12 Aug 2019 19:26:36: 31000000 INFO @ Mon, 12 Aug 2019 19:26:45: 32000000 INFO @ Mon, 12 Aug 2019 19:26:45: 36000000 INFO @ Mon, 12 Aug 2019 19:26:46: 32000000 INFO @ Mon, 12 Aug 2019 19:26:52: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:26:52: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:26:52: #1 total tags in treatment: 36621967 INFO @ Mon, 12 Aug 2019 19:26:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:26:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:26:52: #1 tags after filtering in treatment: 36621967 INFO @ Mon, 12 Aug 2019 19:26:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:26:52: #1 finished! INFO @ Mon, 12 Aug 2019 19:26:52: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:26:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:26:55: 33000000 INFO @ Mon, 12 Aug 2019 19:26:55: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:26:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:26:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:26:56: 33000000 INFO @ Mon, 12 Aug 2019 19:27:05: 34000000 INFO @ Mon, 12 Aug 2019 19:27:06: 34000000 INFO @ Mon, 12 Aug 2019 19:27:15: 35000000 INFO @ Mon, 12 Aug 2019 19:27:16: 35000000 INFO @ Mon, 12 Aug 2019 19:27:25: 36000000 INFO @ Mon, 12 Aug 2019 19:27:26: 36000000 INFO @ Mon, 12 Aug 2019 19:27:32: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:27:32: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:27:32: #1 total tags in treatment: 36621967 INFO @ Mon, 12 Aug 2019 19:27:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:27:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:27:32: #1 tags after filtering in treatment: 36621967 INFO @ Mon, 12 Aug 2019 19:27:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:27:32: #1 finished! INFO @ Mon, 12 Aug 2019 19:27:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:27:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:27:33: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:27:33: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:27:33: #1 total tags in treatment: 36621967 INFO @ Mon, 12 Aug 2019 19:27:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:27:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:27:34: #1 tags after filtering in treatment: 36621967 INFO @ Mon, 12 Aug 2019 19:27:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:27:34: #1 finished! INFO @ Mon, 12 Aug 2019 19:27:34: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:27:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:27:35: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:27:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:27:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:27:36: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:27:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:27:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX466564/SRX466564.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。