Job ID = 1292470 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,166,289 reads read : 27,166,289 reads written : 27,166,289 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:57 27166289 reads; of these: 27166289 (100.00%) were unpaired; of these: 525774 (1.94%) aligned 0 times 22841226 (84.08%) aligned exactly 1 time 3799289 (13.99%) aligned >1 times 98.06% overall alignment rate Time searching: 00:04:57 Overall time: 00:04:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3872405 / 26640515 = 0.1454 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 19:24:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:24:29: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:24:29: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:24:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:24:29: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:24:29: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:24:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:24:29: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:24:29: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:24:38: 1000000 INFO @ Sun, 02 Jun 2019 19:24:38: 1000000 INFO @ Sun, 02 Jun 2019 19:24:40: 1000000 INFO @ Sun, 02 Jun 2019 19:24:47: 2000000 INFO @ Sun, 02 Jun 2019 19:24:47: 2000000 INFO @ Sun, 02 Jun 2019 19:24:50: 2000000 INFO @ Sun, 02 Jun 2019 19:24:56: 3000000 INFO @ Sun, 02 Jun 2019 19:24:56: 3000000 INFO @ Sun, 02 Jun 2019 19:25:00: 3000000 INFO @ Sun, 02 Jun 2019 19:25:05: 4000000 INFO @ Sun, 02 Jun 2019 19:25:05: 4000000 INFO @ Sun, 02 Jun 2019 19:25:11: 4000000 INFO @ Sun, 02 Jun 2019 19:25:14: 5000000 INFO @ Sun, 02 Jun 2019 19:25:14: 5000000 INFO @ Sun, 02 Jun 2019 19:25:21: 5000000 INFO @ Sun, 02 Jun 2019 19:25:23: 6000000 INFO @ Sun, 02 Jun 2019 19:25:23: 6000000 INFO @ Sun, 02 Jun 2019 19:25:32: 6000000 INFO @ Sun, 02 Jun 2019 19:25:32: 7000000 INFO @ Sun, 02 Jun 2019 19:25:32: 7000000 INFO @ Sun, 02 Jun 2019 19:25:40: 8000000 INFO @ Sun, 02 Jun 2019 19:25:41: 8000000 INFO @ Sun, 02 Jun 2019 19:25:42: 7000000 INFO @ Sun, 02 Jun 2019 19:25:49: 9000000 INFO @ Sun, 02 Jun 2019 19:25:50: 9000000 INFO @ Sun, 02 Jun 2019 19:25:52: 8000000 INFO @ Sun, 02 Jun 2019 19:25:58: 10000000 INFO @ Sun, 02 Jun 2019 19:25:59: 10000000 INFO @ Sun, 02 Jun 2019 19:26:02: 9000000 INFO @ Sun, 02 Jun 2019 19:26:07: 11000000 INFO @ Sun, 02 Jun 2019 19:26:08: 11000000 INFO @ Sun, 02 Jun 2019 19:26:13: 10000000 INFO @ Sun, 02 Jun 2019 19:26:15: 12000000 INFO @ Sun, 02 Jun 2019 19:26:17: 12000000 INFO @ Sun, 02 Jun 2019 19:26:23: 11000000 INFO @ Sun, 02 Jun 2019 19:26:24: 13000000 INFO @ Sun, 02 Jun 2019 19:26:26: 13000000 INFO @ Sun, 02 Jun 2019 19:26:33: 14000000 INFO @ Sun, 02 Jun 2019 19:26:34: 12000000 INFO @ Sun, 02 Jun 2019 19:26:35: 14000000 INFO @ Sun, 02 Jun 2019 19:26:41: 15000000 INFO @ Sun, 02 Jun 2019 19:26:44: 15000000 INFO @ Sun, 02 Jun 2019 19:26:44: 13000000 INFO @ Sun, 02 Jun 2019 19:26:50: 16000000 INFO @ Sun, 02 Jun 2019 19:26:52: 16000000 INFO @ Sun, 02 Jun 2019 19:26:55: 14000000 INFO @ Sun, 02 Jun 2019 19:26:58: 17000000 INFO @ Sun, 02 Jun 2019 19:27:01: 17000000 INFO @ Sun, 02 Jun 2019 19:27:05: 15000000 INFO @ Sun, 02 Jun 2019 19:27:07: 18000000 INFO @ Sun, 02 Jun 2019 19:27:10: 18000000 INFO @ Sun, 02 Jun 2019 19:27:15: 16000000 INFO @ Sun, 02 Jun 2019 19:27:16: 19000000 INFO @ Sun, 02 Jun 2019 19:27:19: 19000000 INFO @ Sun, 02 Jun 2019 19:27:25: 20000000 INFO @ Sun, 02 Jun 2019 19:27:25: 17000000 INFO @ Sun, 02 Jun 2019 19:27:29: 20000000 INFO @ Sun, 02 Jun 2019 19:27:34: 21000000 INFO @ Sun, 02 Jun 2019 19:27:36: 18000000 INFO @ Sun, 02 Jun 2019 19:27:38: 21000000 INFO @ Sun, 02 Jun 2019 19:27:42: 22000000 INFO @ Sun, 02 Jun 2019 19:27:47: 19000000 INFO @ Sun, 02 Jun 2019 19:27:47: 22000000 INFO @ Sun, 02 Jun 2019 19:27:49: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 19:27:49: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 19:27:49: #1 total tags in treatment: 22768110 INFO @ Sun, 02 Jun 2019 19:27:49: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:27:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:27:50: #1 tags after filtering in treatment: 22768110 INFO @ Sun, 02 Jun 2019 19:27:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:27:50: #1 finished! INFO @ Sun, 02 Jun 2019 19:27:50: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:27:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:27:52: #2 number of paired peaks: 92 WARNING @ Sun, 02 Jun 2019 19:27:52: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 19:27:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:27:54: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 19:27:54: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 19:27:54: #1 total tags in treatment: 22768110 INFO @ Sun, 02 Jun 2019 19:27:54: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:27:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:27:54: #1 tags after filtering in treatment: 22768110 INFO @ Sun, 02 Jun 2019 19:27:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:27:54: #1 finished! INFO @ Sun, 02 Jun 2019 19:27:54: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:27:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:27:56: #2 number of paired peaks: 92 WARNING @ Sun, 02 Jun 2019 19:27:56: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 19:27:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:27:57: 20000000 INFO @ Sun, 02 Jun 2019 19:28:07: 21000000 INFO @ Sun, 02 Jun 2019 19:28:16: 22000000 INFO @ Sun, 02 Jun 2019 19:28:24: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 19:28:24: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 19:28:24: #1 total tags in treatment: 22768110 INFO @ Sun, 02 Jun 2019 19:28:24: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:28:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:28:24: #1 tags after filtering in treatment: 22768110 INFO @ Sun, 02 Jun 2019 19:28:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:28:24: #1 finished! INFO @ Sun, 02 Jun 2019 19:28:24: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:28:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:28:26: #2 number of paired peaks: 92 WARNING @ Sun, 02 Jun 2019 19:28:26: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 19:28:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX463083/SRX463083.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。