Job ID = 10924591 sra ファイルのダウンロード中... Completed: 1208137K bytes transferred in 47 seconds (209861K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 30593783 spots for /home/okishinya/chipatlas/results/ce10/SRX4200538/SRR7298004.sra Written 30593783 spots for /home/okishinya/chipatlas/results/ce10/SRX4200538/SRR7298004.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:58 30593783 reads; of these: 30593783 (100.00%) were unpaired; of these: 733992 (2.40%) aligned 0 times 25781911 (84.27%) aligned exactly 1 time 4077880 (13.33%) aligned >1 times 97.60% overall alignment rate Time searching: 00:10:59 Overall time: 00:10:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15763986 / 29859791 = 0.5279 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 06 Aug 2018 10:45:56: # Command line: callpeak -t SRX4200538.bam -f BAM -g ce -n SRX4200538.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4200538.10 # format = BAM # ChIP-seq file = ['SRX4200538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 10:45:56: # Command line: callpeak -t SRX4200538.bam -f BAM -g ce -n SRX4200538.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4200538.05 # format = BAM # ChIP-seq file = ['SRX4200538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 10:45:56: # Command line: callpeak -t SRX4200538.bam -f BAM -g ce -n SRX4200538.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4200538.20 # format = BAM # ChIP-seq file = ['SRX4200538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 10:45:56: #1 read tag files... INFO @ Mon, 06 Aug 2018 10:45:56: #1 read tag files... INFO @ Mon, 06 Aug 2018 10:45:56: #1 read tag files... INFO @ Mon, 06 Aug 2018 10:45:56: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 10:45:56: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 10:45:56: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 10:46:04: 1000000 INFO @ Mon, 06 Aug 2018 10:46:04: 1000000 INFO @ Mon, 06 Aug 2018 10:46:04: 1000000 INFO @ Mon, 06 Aug 2018 10:46:13: 2000000 INFO @ Mon, 06 Aug 2018 10:46:13: 2000000 INFO @ Mon, 06 Aug 2018 10:46:13: 2000000 INFO @ Mon, 06 Aug 2018 10:46:21: 3000000 INFO @ Mon, 06 Aug 2018 10:46:21: 3000000 INFO @ Mon, 06 Aug 2018 10:46:21: 3000000 INFO @ Mon, 06 Aug 2018 10:46:29: 4000000 INFO @ Mon, 06 Aug 2018 10:46:29: 4000000 INFO @ Mon, 06 Aug 2018 10:46:29: 4000000 INFO @ Mon, 06 Aug 2018 10:46:38: 5000000 INFO @ Mon, 06 Aug 2018 10:46:38: 5000000 INFO @ Mon, 06 Aug 2018 10:46:38: 5000000 INFO @ Mon, 06 Aug 2018 10:46:46: 6000000 INFO @ Mon, 06 Aug 2018 10:46:46: 6000000 INFO @ Mon, 06 Aug 2018 10:46:46: 6000000 INFO @ Mon, 06 Aug 2018 10:46:54: 7000000 INFO @ Mon, 06 Aug 2018 10:46:54: 7000000 INFO @ Mon, 06 Aug 2018 10:46:54: 7000000 INFO @ Mon, 06 Aug 2018 10:47:03: 8000000 INFO @ Mon, 06 Aug 2018 10:47:03: 8000000 INFO @ Mon, 06 Aug 2018 10:47:03: 8000000 INFO @ Mon, 06 Aug 2018 10:47:11: 9000000 INFO @ Mon, 06 Aug 2018 10:47:11: 9000000 INFO @ Mon, 06 Aug 2018 10:47:11: 9000000 INFO @ Mon, 06 Aug 2018 10:47:19: 10000000 INFO @ Mon, 06 Aug 2018 10:47:19: 10000000 INFO @ Mon, 06 Aug 2018 10:47:19: 10000000 INFO @ Mon, 06 Aug 2018 10:47:28: 11000000 INFO @ Mon, 06 Aug 2018 10:47:28: 11000000 INFO @ Mon, 06 Aug 2018 10:47:28: 11000000 INFO @ Mon, 06 Aug 2018 10:47:36: 12000000 INFO @ Mon, 06 Aug 2018 10:47:36: 12000000 INFO @ Mon, 06 Aug 2018 10:47:36: 12000000 INFO @ Mon, 06 Aug 2018 10:47:45: 13000000 INFO @ Mon, 06 Aug 2018 10:47:45: 13000000 INFO @ Mon, 06 Aug 2018 10:47:45: 13000000 INFO @ Mon, 06 Aug 2018 10:47:53: 14000000 INFO @ Mon, 06 Aug 2018 10:47:53: 14000000 INFO @ Mon, 06 Aug 2018 10:47:53: 14000000 INFO @ Mon, 06 Aug 2018 10:47:54: #1 tag size is determined as 75 bps INFO @ Mon, 06 Aug 2018 10:47:54: #1 tag size = 75 INFO @ Mon, 06 Aug 2018 10:47:54: #1 total tags in treatment: 14095805 INFO @ Mon, 06 Aug 2018 10:47:54: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 10:47:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 10:47:54: #1 tag size is determined as 75 bps INFO @ Mon, 06 Aug 2018 10:47:54: #1 tag size = 75 INFO @ Mon, 06 Aug 2018 10:47:54: #1 total tags in treatment: 14095805 INFO @ Mon, 06 Aug 2018 10:47:54: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 10:47:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 10:47:54: #1 tag size is determined as 75 bps INFO @ Mon, 06 Aug 2018 10:47:54: #1 tag size = 75 INFO @ Mon, 06 Aug 2018 10:47:54: #1 total tags in treatment: 14095805 INFO @ Mon, 06 Aug 2018 10:47:54: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 10:47:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 10:47:54: #1 tags after filtering in treatment: 14095805 INFO @ Mon, 06 Aug 2018 10:47:54: #1 tags after filtering in treatment: 14095805 INFO @ Mon, 06 Aug 2018 10:47:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 10:47:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 10:47:54: #1 finished! INFO @ Mon, 06 Aug 2018 10:47:54: #1 finished! INFO @ Mon, 06 Aug 2018 10:47:54: #1 tags after filtering in treatment: 14095805 INFO @ Mon, 06 Aug 2018 10:47:54: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 10:47:54: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 10:47:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 10:47:54: #1 finished! INFO @ Mon, 06 Aug 2018 10:47:54: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 10:47:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 10:47:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 10:47:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 10:47:55: #2 number of paired peaks: 55 WARNING @ Mon, 06 Aug 2018 10:47:55: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Aug 2018 10:47:55: Process for pairing-model is terminated! INFO @ Mon, 06 Aug 2018 10:47:55: #2 number of paired peaks: 55 WARNING @ Mon, 06 Aug 2018 10:47:55: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Aug 2018 10:47:55: Process for pairing-model is terminated! cat: SRX4200538.20_peaks.narrowPeakcat: SRX4200538.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません INFO @ Mon, 06 Aug 2018 10:47:55: #2 number of paired peaks: 55 WARNING @ Mon, 06 Aug 2018 10:47:55: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Aug 2018 10:47:55: Process for pairing-model is terminated! cat: SRX4200538.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4200538.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4200538.20_model.r'rm: cannot remove `SRX4200538.10_*.xls': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX4200538.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4200538.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4200538.20_peaks.narrowPeak'CompletedMACS2peakCalling : そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4200538.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4200538.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4200538.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。