Job ID = 12264784 SRX = SRX4082390 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29195480 spots for SRR7164213/SRR7164213.sra Written 29195480 spots for SRR7164213/SRR7164213.sra Read 38848505 spots for SRR7164214/SRR7164214.sra Written 38848505 spots for SRR7164214/SRR7164214.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265516 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:53:33 68043985 reads; of these: 68043985 (100.00%) were paired; of these: 6415915 (9.43%) aligned concordantly 0 times 55487689 (81.55%) aligned concordantly exactly 1 time 6140381 (9.02%) aligned concordantly >1 times ---- 6415915 pairs aligned concordantly 0 times; of these: 3025883 (47.16%) aligned discordantly 1 time ---- 3390032 pairs aligned 0 times concordantly or discordantly; of these: 6780064 mates make up the pairs; of these: 4880909 (71.99%) aligned 0 times 1152475 (17.00%) aligned exactly 1 time 746680 (11.01%) aligned >1 times 96.41% overall alignment rate Time searching: 00:53:33 Overall time: 00:53:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 26696071 / 62054743 = 0.4302 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:26:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:26:04: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:26:04: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:26:10: 1000000 INFO @ Sat, 03 Apr 2021 07:26:17: 2000000 INFO @ Sat, 03 Apr 2021 07:26:23: 3000000 INFO @ Sat, 03 Apr 2021 07:26:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:26:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:26:33: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:26:33: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:26:35: 5000000 INFO @ Sat, 03 Apr 2021 07:26:39: 1000000 INFO @ Sat, 03 Apr 2021 07:26:42: 6000000 INFO @ Sat, 03 Apr 2021 07:26:46: 2000000 INFO @ Sat, 03 Apr 2021 07:26:49: 7000000 INFO @ Sat, 03 Apr 2021 07:26:52: 3000000 INFO @ Sat, 03 Apr 2021 07:26:55: 8000000 INFO @ Sat, 03 Apr 2021 07:26:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:27:01: 9000000 INFO @ Sat, 03 Apr 2021 07:27:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:27:03: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:27:03: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:27:06: 5000000 INFO @ Sat, 03 Apr 2021 07:27:07: 10000000 INFO @ Sat, 03 Apr 2021 07:27:10: 1000000 INFO @ Sat, 03 Apr 2021 07:27:13: 6000000 INFO @ Sat, 03 Apr 2021 07:27:14: 11000000 INFO @ Sat, 03 Apr 2021 07:27:17: 2000000 INFO @ Sat, 03 Apr 2021 07:27:20: 7000000 INFO @ Sat, 03 Apr 2021 07:27:21: 12000000 INFO @ Sat, 03 Apr 2021 07:27:24: 3000000 INFO @ Sat, 03 Apr 2021 07:27:27: 8000000 INFO @ Sat, 03 Apr 2021 07:27:27: 13000000 INFO @ Sat, 03 Apr 2021 07:27:30: 4000000 INFO @ Sat, 03 Apr 2021 07:27:34: 9000000 INFO @ Sat, 03 Apr 2021 07:27:34: 14000000 INFO @ Sat, 03 Apr 2021 07:27:38: 5000000 INFO @ Sat, 03 Apr 2021 07:27:40: 15000000 INFO @ Sat, 03 Apr 2021 07:27:41: 10000000 INFO @ Sat, 03 Apr 2021 07:27:45: 6000000 INFO @ Sat, 03 Apr 2021 07:27:47: 16000000 INFO @ Sat, 03 Apr 2021 07:27:48: 11000000 INFO @ Sat, 03 Apr 2021 07:27:51: 7000000 INFO @ Sat, 03 Apr 2021 07:27:54: 17000000 INFO @ Sat, 03 Apr 2021 07:27:54: 12000000 INFO @ Sat, 03 Apr 2021 07:27:58: 8000000 INFO @ Sat, 03 Apr 2021 07:28:00: 18000000 INFO @ Sat, 03 Apr 2021 07:28:01: 13000000 INFO @ Sat, 03 Apr 2021 07:28:05: 9000000 INFO @ Sat, 03 Apr 2021 07:28:07: 19000000 INFO @ Sat, 03 Apr 2021 07:28:07: 14000000 INFO @ Sat, 03 Apr 2021 07:28:11: 10000000 INFO @ Sat, 03 Apr 2021 07:28:14: 20000000 INFO @ Sat, 03 Apr 2021 07:28:14: 15000000 INFO @ Sat, 03 Apr 2021 07:28:18: 11000000 INFO @ Sat, 03 Apr 2021 07:28:21: 16000000 INFO @ Sat, 03 Apr 2021 07:28:21: 21000000 INFO @ Sat, 03 Apr 2021 07:28:24: 12000000 INFO @ Sat, 03 Apr 2021 07:28:27: 17000000 INFO @ Sat, 03 Apr 2021 07:28:27: 22000000 INFO @ Sat, 03 Apr 2021 07:28:31: 13000000 INFO @ Sat, 03 Apr 2021 07:28:34: 23000000 INFO @ Sat, 03 Apr 2021 07:28:35: 18000000 INFO @ Sat, 03 Apr 2021 07:28:38: 14000000 INFO @ Sat, 03 Apr 2021 07:28:41: 24000000 INFO @ Sat, 03 Apr 2021 07:28:42: 19000000 INFO @ Sat, 03 Apr 2021 07:28:45: 15000000 INFO @ Sat, 03 Apr 2021 07:28:47: 25000000 INFO @ Sat, 03 Apr 2021 07:28:49: 20000000 INFO @ Sat, 03 Apr 2021 07:28:53: 16000000 INFO @ Sat, 03 Apr 2021 07:28:54: 26000000 INFO @ Sat, 03 Apr 2021 07:28:55: 21000000 INFO @ Sat, 03 Apr 2021 07:28:59: 17000000 INFO @ Sat, 03 Apr 2021 07:29:00: 27000000 INFO @ Sat, 03 Apr 2021 07:29:02: 22000000 INFO @ Sat, 03 Apr 2021 07:29:06: 18000000 INFO @ Sat, 03 Apr 2021 07:29:06: 28000000 INFO @ Sat, 03 Apr 2021 07:29:08: 23000000 INFO @ Sat, 03 Apr 2021 07:29:12: 29000000 INFO @ Sat, 03 Apr 2021 07:29:13: 19000000 INFO @ Sat, 03 Apr 2021 07:29:15: 24000000 INFO @ Sat, 03 Apr 2021 07:29:18: 30000000 INFO @ Sat, 03 Apr 2021 07:29:20: 20000000 INFO @ Sat, 03 Apr 2021 07:29:22: 25000000 INFO @ Sat, 03 Apr 2021 07:29:25: 31000000 INFO @ Sat, 03 Apr 2021 07:29:27: 21000000 INFO @ Sat, 03 Apr 2021 07:29:29: 26000000 INFO @ Sat, 03 Apr 2021 07:29:32: 32000000 INFO @ Sat, 03 Apr 2021 07:29:33: 22000000 INFO @ Sat, 03 Apr 2021 07:29:35: 27000000 INFO @ Sat, 03 Apr 2021 07:29:38: 33000000 INFO @ Sat, 03 Apr 2021 07:29:40: 23000000 INFO @ Sat, 03 Apr 2021 07:29:42: 28000000 INFO @ Sat, 03 Apr 2021 07:29:44: 34000000 INFO @ Sat, 03 Apr 2021 07:29:46: 24000000 INFO @ Sat, 03 Apr 2021 07:29:49: 29000000 INFO @ Sat, 03 Apr 2021 07:29:51: 35000000 INFO @ Sat, 03 Apr 2021 07:29:53: 25000000 INFO @ Sat, 03 Apr 2021 07:29:55: 30000000 INFO @ Sat, 03 Apr 2021 07:29:58: 36000000 INFO @ Sat, 03 Apr 2021 07:30:00: 26000000 INFO @ Sat, 03 Apr 2021 07:30:02: 31000000 INFO @ Sat, 03 Apr 2021 07:30:04: 37000000 INFO @ Sat, 03 Apr 2021 07:30:06: 27000000 INFO @ Sat, 03 Apr 2021 07:30:09: 32000000 INFO @ Sat, 03 Apr 2021 07:30:10: 38000000 INFO @ Sat, 03 Apr 2021 07:30:14: 28000000 INFO @ Sat, 03 Apr 2021 07:30:16: 33000000 INFO @ Sat, 03 Apr 2021 07:30:17: 39000000 INFO @ Sat, 03 Apr 2021 07:30:21: 29000000 INFO @ Sat, 03 Apr 2021 07:30:23: 34000000 INFO @ Sat, 03 Apr 2021 07:30:24: 40000000 INFO @ Sat, 03 Apr 2021 07:30:28: 30000000 INFO @ Sat, 03 Apr 2021 07:30:30: 35000000 INFO @ Sat, 03 Apr 2021 07:30:30: 41000000 INFO @ Sat, 03 Apr 2021 07:30:35: 31000000 INFO @ Sat, 03 Apr 2021 07:30:37: 36000000 INFO @ Sat, 03 Apr 2021 07:30:37: 42000000 INFO @ Sat, 03 Apr 2021 07:30:41: 32000000 INFO @ Sat, 03 Apr 2021 07:30:43: 43000000 INFO @ Sat, 03 Apr 2021 07:30:43: 37000000 INFO @ Sat, 03 Apr 2021 07:30:49: 33000000 INFO @ Sat, 03 Apr 2021 07:30:49: 44000000 INFO @ Sat, 03 Apr 2021 07:30:51: 38000000 INFO @ Sat, 03 Apr 2021 07:30:55: 45000000 INFO @ Sat, 03 Apr 2021 07:30:56: 34000000 INFO @ Sat, 03 Apr 2021 07:30:58: 39000000 INFO @ Sat, 03 Apr 2021 07:31:02: 46000000 INFO @ Sat, 03 Apr 2021 07:31:02: 35000000 INFO @ Sat, 03 Apr 2021 07:31:04: 40000000 INFO @ Sat, 03 Apr 2021 07:31:09: 47000000 INFO @ Sat, 03 Apr 2021 07:31:09: 36000000 INFO @ Sat, 03 Apr 2021 07:31:11: 41000000 INFO @ Sat, 03 Apr 2021 07:31:15: 48000000 INFO @ Sat, 03 Apr 2021 07:31:16: 37000000 INFO @ Sat, 03 Apr 2021 07:31:18: 42000000 INFO @ Sat, 03 Apr 2021 07:31:22: 49000000 INFO @ Sat, 03 Apr 2021 07:31:22: 38000000 INFO @ Sat, 03 Apr 2021 07:31:24: 43000000 INFO @ Sat, 03 Apr 2021 07:31:29: 50000000 INFO @ Sat, 03 Apr 2021 07:31:29: 39000000 INFO @ Sat, 03 Apr 2021 07:31:31: 44000000 INFO @ Sat, 03 Apr 2021 07:31:36: 40000000 INFO @ Sat, 03 Apr 2021 07:31:37: 51000000 INFO @ Sat, 03 Apr 2021 07:31:38: 45000000 INFO @ Sat, 03 Apr 2021 07:31:43: 41000000 INFO @ Sat, 03 Apr 2021 07:31:45: 52000000 INFO @ Sat, 03 Apr 2021 07:31:45: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:31:50: 42000000 INFO @ Sat, 03 Apr 2021 07:31:52: 47000000 INFO @ Sat, 03 Apr 2021 07:31:53: 53000000 INFO @ Sat, 03 Apr 2021 07:31:58: 43000000 INFO @ Sat, 03 Apr 2021 07:32:00: 48000000 INFO @ Sat, 03 Apr 2021 07:32:00: 54000000 INFO @ Sat, 03 Apr 2021 07:32:06: 44000000 INFO @ Sat, 03 Apr 2021 07:32:08: 49000000 INFO @ Sat, 03 Apr 2021 07:32:08: 55000000 INFO @ Sat, 03 Apr 2021 07:32:13: 45000000 INFO @ Sat, 03 Apr 2021 07:32:15: 50000000 INFO @ Sat, 03 Apr 2021 07:32:16: 56000000 INFO @ Sat, 03 Apr 2021 07:32:21: 46000000 INFO @ Sat, 03 Apr 2021 07:32:23: 51000000 INFO @ Sat, 03 Apr 2021 07:32:24: 57000000 INFO @ Sat, 03 Apr 2021 07:32:29: 47000000 INFO @ Sat, 03 Apr 2021 07:32:30: 52000000 INFO @ Sat, 03 Apr 2021 07:32:33: 58000000 INFO @ Sat, 03 Apr 2021 07:32:36: 48000000 INFO @ Sat, 03 Apr 2021 07:32:38: 53000000 INFO @ Sat, 03 Apr 2021 07:32:42: 59000000 INFO @ Sat, 03 Apr 2021 07:32:44: 49000000 INFO @ Sat, 03 Apr 2021 07:32:45: 54000000 INFO @ Sat, 03 Apr 2021 07:32:50: 60000000 INFO @ Sat, 03 Apr 2021 07:32:51: 50000000 INFO @ Sat, 03 Apr 2021 07:32:52: 55000000 INFO @ Sat, 03 Apr 2021 07:32:58: 51000000 INFO @ Sat, 03 Apr 2021 07:32:59: 61000000 INFO @ Sat, 03 Apr 2021 07:32:59: 56000000 INFO @ Sat, 03 Apr 2021 07:33:06: 52000000 INFO @ Sat, 03 Apr 2021 07:33:06: 57000000 INFO @ Sat, 03 Apr 2021 07:33:08: 62000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:33:13: 53000000 INFO @ Sat, 03 Apr 2021 07:33:14: 58000000 INFO @ Sat, 03 Apr 2021 07:33:16: 63000000 INFO @ Sat, 03 Apr 2021 07:33:21: 54000000 INFO @ Sat, 03 Apr 2021 07:33:22: 59000000 INFO @ Sat, 03 Apr 2021 07:33:24: 64000000 INFO @ Sat, 03 Apr 2021 07:33:29: 55000000 INFO @ Sat, 03 Apr 2021 07:33:30: 60000000 INFO @ Sat, 03 Apr 2021 07:33:33: 65000000 INFO @ Sat, 03 Apr 2021 07:33:38: 56000000 INFO @ Sat, 03 Apr 2021 07:33:38: 61000000 INFO @ Sat, 03 Apr 2021 07:33:42: 66000000 INFO @ Sat, 03 Apr 2021 07:33:45: 57000000 INFO @ Sat, 03 Apr 2021 07:33:46: 62000000 INFO @ Sat, 03 Apr 2021 07:33:52: 67000000 INFO @ Sat, 03 Apr 2021 07:33:53: 58000000 INFO @ Sat, 03 Apr 2021 07:33:54: 63000000 INFO @ Sat, 03 Apr 2021 07:34:01: 68000000 INFO @ Sat, 03 Apr 2021 07:34:01: 59000000 INFO @ Sat, 03 Apr 2021 07:34:01: 64000000 INFO @ Sat, 03 Apr 2021 07:34:09: 60000000 INFO @ Sat, 03 Apr 2021 07:34:09: 65000000 INFO @ Sat, 03 Apr 2021 07:34:10: 69000000 INFO @ Sat, 03 Apr 2021 07:34:17: 61000000 INFO @ Sat, 03 Apr 2021 07:34:17: 66000000 INFO @ Sat, 03 Apr 2021 07:34:19: 70000000 INFO @ Sat, 03 Apr 2021 07:34:25: 62000000 INFO @ Sat, 03 Apr 2021 07:34:25: 67000000 INFO @ Sat, 03 Apr 2021 07:34:28: 71000000 INFO @ Sat, 03 Apr 2021 07:34:33: 63000000 INFO @ Sat, 03 Apr 2021 07:34:33: 68000000 INFO @ Sat, 03 Apr 2021 07:34:37: 72000000 INFO @ Sat, 03 Apr 2021 07:34:40: 64000000 INFO @ Sat, 03 Apr 2021 07:34:40: 69000000 INFO @ Sat, 03 Apr 2021 07:34:45: 73000000 INFO @ Sat, 03 Apr 2021 07:34:48: 65000000 INFO @ Sat, 03 Apr 2021 07:34:48: 70000000 INFO @ Sat, 03 Apr 2021 07:34:54: 74000000 INFO @ Sat, 03 Apr 2021 07:34:56: 71000000 INFO @ Sat, 03 Apr 2021 07:34:56: 66000000 INFO @ Sat, 03 Apr 2021 07:35:03: 75000000 INFO @ Sat, 03 Apr 2021 07:35:04: 72000000 INFO @ Sat, 03 Apr 2021 07:35:04: 67000000 INFO @ Sat, 03 Apr 2021 07:35:11: 73000000 INFO @ Sat, 03 Apr 2021 07:35:12: 76000000 INFO @ Sat, 03 Apr 2021 07:35:12: 68000000 INFO @ Sat, 03 Apr 2021 07:35:19: 74000000 INFO @ Sat, 03 Apr 2021 07:35:20: 69000000 INFO @ Sat, 03 Apr 2021 07:35:21: 77000000 INFO @ Sat, 03 Apr 2021 07:35:26: 75000000 INFO @ Sat, 03 Apr 2021 07:35:27: 70000000 INFO @ Sat, 03 Apr 2021 07:35:28: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:35:28: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:35:28: #1 total tags in treatment: 35390622 INFO @ Sat, 03 Apr 2021 07:35:28: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:35:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:35:29: #1 tags after filtering in treatment: 18245608 INFO @ Sat, 03 Apr 2021 07:35:29: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 03 Apr 2021 07:35:29: #1 finished! INFO @ Sat, 03 Apr 2021 07:35:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:35:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:35:30: #2 number of paired peaks: 253 WARNING @ Sat, 03 Apr 2021 07:35:30: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Sat, 03 Apr 2021 07:35:30: start model_add_line... INFO @ Sat, 03 Apr 2021 07:35:30: start X-correlation... INFO @ Sat, 03 Apr 2021 07:35:30: end of X-cor INFO @ Sat, 03 Apr 2021 07:35:30: #2 finished! INFO @ Sat, 03 Apr 2021 07:35:30: #2 predicted fragment length is 70 bps INFO @ Sat, 03 Apr 2021 07:35:30: #2 alternative fragment length(s) may be 70 bps INFO @ Sat, 03 Apr 2021 07:35:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.05_model.r WARNING @ Sat, 03 Apr 2021 07:35:30: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:35:30: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Sat, 03 Apr 2021 07:35:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:35:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:35:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:35:33: 76000000 INFO @ Sat, 03 Apr 2021 07:35:35: 71000000 INFO @ Sat, 03 Apr 2021 07:35:41: 77000000 INFO @ Sat, 03 Apr 2021 07:35:42: 72000000 INFO @ Sat, 03 Apr 2021 07:35:47: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:35:47: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:35:47: #1 total tags in treatment: 35390622 INFO @ Sat, 03 Apr 2021 07:35:47: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:35:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:35:48: #1 tags after filtering in treatment: 18245608 INFO @ Sat, 03 Apr 2021 07:35:48: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 03 Apr 2021 07:35:48: #1 finished! INFO @ Sat, 03 Apr 2021 07:35:48: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:35:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:35:49: #2 number of paired peaks: 253 WARNING @ Sat, 03 Apr 2021 07:35:49: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Sat, 03 Apr 2021 07:35:49: start model_add_line... INFO @ Sat, 03 Apr 2021 07:35:49: start X-correlation... INFO @ Sat, 03 Apr 2021 07:35:49: end of X-cor INFO @ Sat, 03 Apr 2021 07:35:49: #2 finished! INFO @ Sat, 03 Apr 2021 07:35:49: #2 predicted fragment length is 70 bps INFO @ Sat, 03 Apr 2021 07:35:49: #2 alternative fragment length(s) may be 70 bps INFO @ Sat, 03 Apr 2021 07:35:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.10_model.r WARNING @ Sat, 03 Apr 2021 07:35:49: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:35:49: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Sat, 03 Apr 2021 07:35:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:35:49: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:35:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:35:49: 73000000 INFO @ Sat, 03 Apr 2021 07:35:56: 74000000 INFO @ Sat, 03 Apr 2021 07:36:03: 75000000 INFO @ Sat, 03 Apr 2021 07:36:03: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:36:10: 76000000 INFO @ Sat, 03 Apr 2021 07:36:16: 77000000 INFO @ Sat, 03 Apr 2021 07:36:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:36:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:36:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.05_summits.bed INFO @ Sat, 03 Apr 2021 07:36:22: Done! INFO @ Sat, 03 Apr 2021 07:36:22: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:36:22: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:36:22: #1 total tags in treatment: 35390622 INFO @ Sat, 03 Apr 2021 07:36:22: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:36:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (14677 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:36:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:36:23: #1 tags after filtering in treatment: 18245608 INFO @ Sat, 03 Apr 2021 07:36:23: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 03 Apr 2021 07:36:23: #1 finished! INFO @ Sat, 03 Apr 2021 07:36:23: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:36:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:36:24: #2 number of paired peaks: 253 WARNING @ Sat, 03 Apr 2021 07:36:24: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Sat, 03 Apr 2021 07:36:24: start model_add_line... INFO @ Sat, 03 Apr 2021 07:36:25: start X-correlation... INFO @ Sat, 03 Apr 2021 07:36:25: end of X-cor INFO @ Sat, 03 Apr 2021 07:36:25: #2 finished! INFO @ Sat, 03 Apr 2021 07:36:25: #2 predicted fragment length is 70 bps INFO @ Sat, 03 Apr 2021 07:36:25: #2 alternative fragment length(s) may be 70 bps INFO @ Sat, 03 Apr 2021 07:36:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.20_model.r WARNING @ Sat, 03 Apr 2021 07:36:25: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:36:25: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Sat, 03 Apr 2021 07:36:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:36:25: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:36:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:36:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:36:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:36:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.10_summits.bed INFO @ Sat, 03 Apr 2021 07:36:42: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (8759 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:36:58: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:37:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:37:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:37:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082390/SRX4082390.20_summits.bed INFO @ Sat, 03 Apr 2021 07:37:17: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (3334 records, 4 fields): 5 millis CompletedMACS2peakCalling