Job ID = 1290655 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,002,611 reads read : 24,005,222 reads written : 12,002,611 reads 0-length : 12,002,611 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:49 12002611 reads; of these: 12002611 (100.00%) were unpaired; of these: 1494872 (12.45%) aligned 0 times 9294605 (77.44%) aligned exactly 1 time 1213134 (10.11%) aligned >1 times 87.55% overall alignment rate Time searching: 00:02:49 Overall time: 00:02:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1519280 / 10507739 = 0.1446 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 01 Jun 2019 22:00:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 01 Jun 2019 22:00:14: #1 read tag files... INFO @ Sat, 01 Jun 2019 22:00:14: #1 read treatment tags... INFO @ Sat, 01 Jun 2019 22:00:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 01 Jun 2019 22:00:14: #1 read tag files... INFO @ Sat, 01 Jun 2019 22:00:14: #1 read treatment tags... INFO @ Sat, 01 Jun 2019 22:00:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 01 Jun 2019 22:00:14: #1 read tag files... INFO @ Sat, 01 Jun 2019 22:00:14: #1 read treatment tags... INFO @ Sat, 01 Jun 2019 22:00:23: 1000000 INFO @ Sat, 01 Jun 2019 22:00:23: 1000000 INFO @ Sat, 01 Jun 2019 22:00:24: 1000000 INFO @ Sat, 01 Jun 2019 22:00:31: 2000000 INFO @ Sat, 01 Jun 2019 22:00:31: 2000000 INFO @ Sat, 01 Jun 2019 22:00:33: 2000000 INFO @ Sat, 01 Jun 2019 22:00:39: 3000000 INFO @ Sat, 01 Jun 2019 22:00:39: 3000000 INFO @ Sat, 01 Jun 2019 22:00:42: 3000000 INFO @ Sat, 01 Jun 2019 22:00:47: 4000000 INFO @ Sat, 01 Jun 2019 22:00:47: 4000000 INFO @ Sat, 01 Jun 2019 22:00:51: 4000000 INFO @ Sat, 01 Jun 2019 22:00:56: 5000000 INFO @ Sat, 01 Jun 2019 22:00:56: 5000000 INFO @ Sat, 01 Jun 2019 22:01:00: 5000000 INFO @ Sat, 01 Jun 2019 22:01:04: 6000000 INFO @ Sat, 01 Jun 2019 22:01:05: 6000000 INFO @ Sat, 01 Jun 2019 22:01:10: 6000000 INFO @ Sat, 01 Jun 2019 22:01:13: 7000000 INFO @ Sat, 01 Jun 2019 22:01:13: 7000000 INFO @ Sat, 01 Jun 2019 22:01:19: 7000000 INFO @ Sat, 01 Jun 2019 22:01:21: 8000000 INFO @ Sat, 01 Jun 2019 22:01:22: 8000000 INFO @ Sat, 01 Jun 2019 22:01:28: 8000000 INFO @ Sat, 01 Jun 2019 22:01:30: #1 tag size is determined as 50 bps INFO @ Sat, 01 Jun 2019 22:01:30: #1 tag size = 50 INFO @ Sat, 01 Jun 2019 22:01:30: #1 total tags in treatment: 8988459 INFO @ Sat, 01 Jun 2019 22:01:30: #1 user defined the maximum tags... INFO @ Sat, 01 Jun 2019 22:01:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 01 Jun 2019 22:01:30: #1 tag size is determined as 50 bps INFO @ Sat, 01 Jun 2019 22:01:30: #1 tag size = 50 INFO @ Sat, 01 Jun 2019 22:01:30: #1 total tags in treatment: 8988459 INFO @ Sat, 01 Jun 2019 22:01:30: #1 user defined the maximum tags... INFO @ Sat, 01 Jun 2019 22:01:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 01 Jun 2019 22:01:30: #1 tags after filtering in treatment: 8988459 INFO @ Sat, 01 Jun 2019 22:01:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 01 Jun 2019 22:01:30: #1 finished! INFO @ Sat, 01 Jun 2019 22:01:30: #2 Build Peak Model... INFO @ Sat, 01 Jun 2019 22:01:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 01 Jun 2019 22:01:30: #1 tags after filtering in treatment: 8988459 INFO @ Sat, 01 Jun 2019 22:01:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 01 Jun 2019 22:01:30: #1 finished! INFO @ Sat, 01 Jun 2019 22:01:30: #2 Build Peak Model... INFO @ Sat, 01 Jun 2019 22:01:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 01 Jun 2019 22:01:31: #2 number of paired peaks: 90 WARNING @ Sat, 01 Jun 2019 22:01:31: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 01 Jun 2019 22:01:31: Process for pairing-model is terminated! INFO @ Sat, 01 Jun 2019 22:01:31: #2 number of paired peaks: 90 WARNING @ Sat, 01 Jun 2019 22:01:31: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 01 Jun 2019 22:01:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 01 Jun 2019 22:01:37: #1 tag size is determined as 50 bps INFO @ Sat, 01 Jun 2019 22:01:37: #1 tag size = 50 INFO @ Sat, 01 Jun 2019 22:01:37: #1 total tags in treatment: 8988459 INFO @ Sat, 01 Jun 2019 22:01:37: #1 user defined the maximum tags... INFO @ Sat, 01 Jun 2019 22:01:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 01 Jun 2019 22:01:37: #1 tags after filtering in treatment: 8988459 INFO @ Sat, 01 Jun 2019 22:01:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 01 Jun 2019 22:01:37: #1 finished! INFO @ Sat, 01 Jun 2019 22:01:37: #2 Build Peak Model... INFO @ Sat, 01 Jun 2019 22:01:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 01 Jun 2019 22:01:38: #2 number of paired peaks: 90 WARNING @ Sat, 01 Jun 2019 22:01:38: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 01 Jun 2019 22:01:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX4082348/SRX4082348.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。