Job ID = 11158465 sra ファイルのダウンロード中... Completed: 3202492K bytes transferred in 333 seconds (78557K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 40493711 spots for /home/okishinya/chipatlas/results/ce10/SRX4012479/SRR7082720.sra Written 40493711 spots for /home/okishinya/chipatlas/results/ce10/SRX4012479/SRR7082720.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:01:58 40493711 reads; of these: 40493711 (100.00%) were paired; of these: 1524236 (3.76%) aligned concordantly 0 times 33596075 (82.97%) aligned concordantly exactly 1 time 5373400 (13.27%) aligned concordantly >1 times ---- 1524236 pairs aligned concordantly 0 times; of these: 599049 (39.30%) aligned discordantly 1 time ---- 925187 pairs aligned 0 times concordantly or discordantly; of these: 1850374 mates make up the pairs; of these: 780985 (42.21%) aligned 0 times 617282 (33.36%) aligned exactly 1 time 452107 (24.43%) aligned >1 times 99.04% overall alignment rate Time searching: 01:01:59 Overall time: 01:01:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 1599245 / 39090940 = 0.0409 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 04 Sep 2018 10:08:32: # Command line: callpeak -t SRX4012479.bam -f BAM -g ce -n SRX4012479.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4012479.20 # format = BAM # ChIP-seq file = ['SRX4012479.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:08:32: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:08:32: # Command line: callpeak -t SRX4012479.bam -f BAM -g ce -n SRX4012479.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4012479.10 # format = BAM # ChIP-seq file = ['SRX4012479.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:08:32: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:08:32: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:08:32: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:08:32: # Command line: callpeak -t SRX4012479.bam -f BAM -g ce -n SRX4012479.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4012479.05 # format = BAM # ChIP-seq file = ['SRX4012479.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:08:32: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:08:32: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:08:40: 1000000 INFO @ Tue, 04 Sep 2018 10:08:40: 1000000 INFO @ Tue, 04 Sep 2018 10:08:40: 1000000 INFO @ Tue, 04 Sep 2018 10:08:47: 2000000 INFO @ Tue, 04 Sep 2018 10:08:47: 2000000 INFO @ Tue, 04 Sep 2018 10:08:47: 2000000 INFO @ Tue, 04 Sep 2018 10:08:55: 3000000 INFO @ Tue, 04 Sep 2018 10:08:55: 3000000 INFO @ Tue, 04 Sep 2018 10:08:55: 3000000 INFO @ Tue, 04 Sep 2018 10:09:02: 4000000 INFO @ Tue, 04 Sep 2018 10:09:02: 4000000 INFO @ Tue, 04 Sep 2018 10:09:02: 4000000 INFO @ Tue, 04 Sep 2018 10:09:10: 5000000 INFO @ Tue, 04 Sep 2018 10:09:10: 5000000 INFO @ Tue, 04 Sep 2018 10:09:10: 5000000 INFO @ Tue, 04 Sep 2018 10:09:17: 6000000 INFO @ Tue, 04 Sep 2018 10:09:17: 6000000 INFO @ Tue, 04 Sep 2018 10:09:17: 6000000 INFO @ Tue, 04 Sep 2018 10:09:24: 7000000 INFO @ Tue, 04 Sep 2018 10:09:24: 7000000 INFO @ Tue, 04 Sep 2018 10:09:24: 7000000 INFO @ Tue, 04 Sep 2018 10:09:32: 8000000 INFO @ Tue, 04 Sep 2018 10:09:32: 8000000 INFO @ Tue, 04 Sep 2018 10:09:32: 8000000 INFO @ Tue, 04 Sep 2018 10:09:39: 9000000 INFO @ Tue, 04 Sep 2018 10:09:39: 9000000 INFO @ Tue, 04 Sep 2018 10:09:39: 9000000 INFO @ Tue, 04 Sep 2018 10:09:46: 10000000 INFO @ Tue, 04 Sep 2018 10:09:46: 10000000 INFO @ Tue, 04 Sep 2018 10:09:47: 10000000 INFO @ Tue, 04 Sep 2018 10:09:54: 11000000 INFO @ Tue, 04 Sep 2018 10:09:54: 11000000 INFO @ Tue, 04 Sep 2018 10:09:54: 11000000 INFO @ Tue, 04 Sep 2018 10:10:01: 12000000 INFO @ Tue, 04 Sep 2018 10:10:01: 12000000 INFO @ Tue, 04 Sep 2018 10:10:02: 12000000 INFO @ Tue, 04 Sep 2018 10:10:08: 13000000 INFO @ Tue, 04 Sep 2018 10:10:08: 13000000 INFO @ Tue, 04 Sep 2018 10:10:09: 13000000 INFO @ Tue, 04 Sep 2018 10:10:16: 14000000 INFO @ Tue, 04 Sep 2018 10:10:16: 14000000 INFO @ Tue, 04 Sep 2018 10:10:17: 14000000 INFO @ Tue, 04 Sep 2018 10:10:23: 15000000 INFO @ Tue, 04 Sep 2018 10:10:23: 15000000 INFO @ Tue, 04 Sep 2018 10:10:24: 15000000 INFO @ Tue, 04 Sep 2018 10:10:30: 16000000 INFO @ Tue, 04 Sep 2018 10:10:30: 16000000 INFO @ Tue, 04 Sep 2018 10:10:32: 16000000 INFO @ Tue, 04 Sep 2018 10:10:37: 17000000 INFO @ Tue, 04 Sep 2018 10:10:37: 17000000 INFO @ Tue, 04 Sep 2018 10:10:39: 17000000 INFO @ Tue, 04 Sep 2018 10:10:45: 18000000 INFO @ Tue, 04 Sep 2018 10:10:45: 18000000 INFO @ Tue, 04 Sep 2018 10:10:46: 18000000 INFO @ Tue, 04 Sep 2018 10:10:52: 19000000 INFO @ Tue, 04 Sep 2018 10:10:52: 19000000 INFO @ Tue, 04 Sep 2018 10:10:54: 19000000 INFO @ Tue, 04 Sep 2018 10:10:59: 20000000 INFO @ Tue, 04 Sep 2018 10:10:59: 20000000 INFO @ Tue, 04 Sep 2018 10:11:01: 20000000 INFO @ Tue, 04 Sep 2018 10:11:06: 21000000 INFO @ Tue, 04 Sep 2018 10:11:06: 21000000 INFO @ Tue, 04 Sep 2018 10:11:08: 21000000 INFO @ Tue, 04 Sep 2018 10:11:14: 22000000 INFO @ Tue, 04 Sep 2018 10:11:14: 22000000 INFO @ Tue, 04 Sep 2018 10:11:16: 22000000 INFO @ Tue, 04 Sep 2018 10:11:21: 23000000 INFO @ Tue, 04 Sep 2018 10:11:21: 23000000 INFO @ Tue, 04 Sep 2018 10:11:23: 23000000 INFO @ Tue, 04 Sep 2018 10:11:28: 24000000 INFO @ Tue, 04 Sep 2018 10:11:28: 24000000 INFO @ Tue, 04 Sep 2018 10:11:30: 24000000 INFO @ Tue, 04 Sep 2018 10:11:36: 25000000 INFO @ Tue, 04 Sep 2018 10:11:36: 25000000 INFO @ Tue, 04 Sep 2018 10:11:38: 25000000 INFO @ Tue, 04 Sep 2018 10:11:43: 26000000 INFO @ Tue, 04 Sep 2018 10:11:43: 26000000 INFO @ Tue, 04 Sep 2018 10:11:45: 26000000 INFO @ Tue, 04 Sep 2018 10:11:50: 27000000 INFO @ Tue, 04 Sep 2018 10:11:50: 27000000 INFO @ Tue, 04 Sep 2018 10:11:53: 27000000 INFO @ Tue, 04 Sep 2018 10:11:58: 28000000 INFO @ Tue, 04 Sep 2018 10:11:58: 28000000 INFO @ Tue, 04 Sep 2018 10:12:00: 28000000 INFO @ Tue, 04 Sep 2018 10:12:05: 29000000 INFO @ Tue, 04 Sep 2018 10:12:05: 29000000 INFO @ Tue, 04 Sep 2018 10:12:08: 29000000 INFO @ Tue, 04 Sep 2018 10:12:12: 30000000 INFO @ Tue, 04 Sep 2018 10:12:12: 30000000 INFO @ Tue, 04 Sep 2018 10:12:15: 30000000 INFO @ Tue, 04 Sep 2018 10:12:19: 31000000 INFO @ Tue, 04 Sep 2018 10:12:19: 31000000 INFO @ Tue, 04 Sep 2018 10:12:22: 31000000 INFO @ Tue, 04 Sep 2018 10:12:27: 32000000 INFO @ Tue, 04 Sep 2018 10:12:27: 32000000 INFO @ Tue, 04 Sep 2018 10:12:30: 32000000 INFO @ Tue, 04 Sep 2018 10:12:34: 33000000 INFO @ Tue, 04 Sep 2018 10:12:34: 33000000 INFO @ Tue, 04 Sep 2018 10:12:37: 33000000 INFO @ Tue, 04 Sep 2018 10:12:41: 34000000 INFO @ Tue, 04 Sep 2018 10:12:41: 34000000 INFO @ Tue, 04 Sep 2018 10:12:44: 34000000 INFO @ Tue, 04 Sep 2018 10:12:49: 35000000 INFO @ Tue, 04 Sep 2018 10:12:49: 35000000 INFO @ Tue, 04 Sep 2018 10:12:52: 35000000 INFO @ Tue, 04 Sep 2018 10:12:56: 36000000 INFO @ Tue, 04 Sep 2018 10:12:56: 36000000 INFO @ Tue, 04 Sep 2018 10:12:59: 36000000 INFO @ Tue, 04 Sep 2018 10:13:03: 37000000 INFO @ Tue, 04 Sep 2018 10:13:03: 37000000 INFO @ Tue, 04 Sep 2018 10:13:07: 37000000 INFO @ Tue, 04 Sep 2018 10:13:10: 38000000 INFO @ Tue, 04 Sep 2018 10:13:10: 38000000 INFO @ Tue, 04 Sep 2018 10:13:14: 38000000 INFO @ Tue, 04 Sep 2018 10:13:18: 39000000 INFO @ Tue, 04 Sep 2018 10:13:18: 39000000 INFO @ Tue, 04 Sep 2018 10:13:21: 39000000 INFO @ Tue, 04 Sep 2018 10:13:25: 40000000 INFO @ Tue, 04 Sep 2018 10:13:25: 40000000 INFO @ Tue, 04 Sep 2018 10:13:29: 40000000 INFO @ Tue, 04 Sep 2018 10:13:32: 41000000 INFO @ Tue, 04 Sep 2018 10:13:32: 41000000 INFO @ Tue, 04 Sep 2018 10:13:36: 41000000 INFO @ Tue, 04 Sep 2018 10:13:39: 42000000 INFO @ Tue, 04 Sep 2018 10:13:39: 42000000 INFO @ Tue, 04 Sep 2018 10:13:43: 42000000 INFO @ Tue, 04 Sep 2018 10:13:46: 43000000 INFO @ Tue, 04 Sep 2018 10:13:46: 43000000 INFO @ Tue, 04 Sep 2018 10:13:51: 43000000 INFO @ Tue, 04 Sep 2018 10:13:54: 44000000 INFO @ Tue, 04 Sep 2018 10:13:54: 44000000 INFO @ Tue, 04 Sep 2018 10:13:58: 44000000 INFO @ Tue, 04 Sep 2018 10:14:01: 45000000 INFO @ Tue, 04 Sep 2018 10:14:01: 45000000 INFO @ Tue, 04 Sep 2018 10:14:05: 45000000 INFO @ Tue, 04 Sep 2018 10:14:08: 46000000 INFO @ Tue, 04 Sep 2018 10:14:08: 46000000 INFO @ Tue, 04 Sep 2018 10:14:13: 46000000 INFO @ Tue, 04 Sep 2018 10:14:16: 47000000 INFO @ Tue, 04 Sep 2018 10:14:16: 47000000 INFO @ Tue, 04 Sep 2018 10:14:20: 47000000 INFO @ Tue, 04 Sep 2018 10:14:23: 48000000 INFO @ Tue, 04 Sep 2018 10:14:23: 48000000 INFO @ Tue, 04 Sep 2018 10:14:27: 48000000 INFO @ Tue, 04 Sep 2018 10:14:30: 49000000 INFO @ Tue, 04 Sep 2018 10:14:30: 49000000 INFO @ Tue, 04 Sep 2018 10:14:35: 49000000 INFO @ Tue, 04 Sep 2018 10:14:37: 50000000 INFO @ Tue, 04 Sep 2018 10:14:37: 50000000 INFO @ Tue, 04 Sep 2018 10:14:42: 50000000 INFO @ Tue, 04 Sep 2018 10:14:45: 51000000 INFO @ Tue, 04 Sep 2018 10:14:45: 51000000 INFO @ Tue, 04 Sep 2018 10:14:49: 51000000 INFO @ Tue, 04 Sep 2018 10:14:52: 52000000 INFO @ Tue, 04 Sep 2018 10:14:52: 52000000 INFO @ Tue, 04 Sep 2018 10:14:57: 52000000 INFO @ Tue, 04 Sep 2018 10:14:59: 53000000 INFO @ Tue, 04 Sep 2018 10:14:59: 53000000 INFO @ Tue, 04 Sep 2018 10:15:04: 53000000 INFO @ Tue, 04 Sep 2018 10:15:07: 54000000 INFO @ Tue, 04 Sep 2018 10:15:07: 54000000 INFO @ Tue, 04 Sep 2018 10:15:11: 54000000 INFO @ Tue, 04 Sep 2018 10:15:14: 55000000 INFO @ Tue, 04 Sep 2018 10:15:14: 55000000 INFO @ Tue, 04 Sep 2018 10:15:19: 55000000 INFO @ Tue, 04 Sep 2018 10:15:21: 56000000 INFO @ Tue, 04 Sep 2018 10:15:21: 56000000 INFO @ Tue, 04 Sep 2018 10:15:26: 56000000 INFO @ Tue, 04 Sep 2018 10:15:28: 57000000 INFO @ Tue, 04 Sep 2018 10:15:28: 57000000 INFO @ Tue, 04 Sep 2018 10:15:33: 57000000 INFO @ Tue, 04 Sep 2018 10:15:36: 58000000 INFO @ Tue, 04 Sep 2018 10:15:36: 58000000 INFO @ Tue, 04 Sep 2018 10:15:41: 58000000 INFO @ Tue, 04 Sep 2018 10:15:43: 59000000 INFO @ Tue, 04 Sep 2018 10:15:43: 59000000 INFO @ Tue, 04 Sep 2018 10:15:48: 59000000 INFO @ Tue, 04 Sep 2018 10:15:50: 60000000 INFO @ Tue, 04 Sep 2018 10:15:50: 60000000 INFO @ Tue, 04 Sep 2018 10:15:55: 60000000 INFO @ Tue, 04 Sep 2018 10:15:57: 61000000 INFO @ Tue, 04 Sep 2018 10:15:57: 61000000 INFO @ Tue, 04 Sep 2018 10:16:03: 61000000 INFO @ Tue, 04 Sep 2018 10:16:05: 62000000 INFO @ Tue, 04 Sep 2018 10:16:05: 62000000 INFO @ Tue, 04 Sep 2018 10:16:10: 62000000 INFO @ Tue, 04 Sep 2018 10:16:12: 63000000 INFO @ Tue, 04 Sep 2018 10:16:12: 63000000 INFO @ Tue, 04 Sep 2018 10:16:18: 63000000 INFO @ Tue, 04 Sep 2018 10:16:19: 64000000 INFO @ Tue, 04 Sep 2018 10:16:19: 64000000 INFO @ Tue, 04 Sep 2018 10:16:25: 64000000 INFO @ Tue, 04 Sep 2018 10:16:27: 65000000 INFO @ Tue, 04 Sep 2018 10:16:27: 65000000 INFO @ Tue, 04 Sep 2018 10:16:32: 65000000 INFO @ Tue, 04 Sep 2018 10:16:34: 66000000 INFO @ Tue, 04 Sep 2018 10:16:34: 66000000 INFO @ Tue, 04 Sep 2018 10:16:40: 66000000 INFO @ Tue, 04 Sep 2018 10:16:41: 67000000 INFO @ Tue, 04 Sep 2018 10:16:41: 67000000 INFO @ Tue, 04 Sep 2018 10:16:47: 67000000 INFO @ Tue, 04 Sep 2018 10:16:48: 68000000 INFO @ Tue, 04 Sep 2018 10:16:48: 68000000 INFO @ Tue, 04 Sep 2018 10:16:54: 68000000 INFO @ Tue, 04 Sep 2018 10:16:56: 69000000 INFO @ Tue, 04 Sep 2018 10:16:56: 69000000 INFO @ Tue, 04 Sep 2018 10:17:02: 69000000 INFO @ Tue, 04 Sep 2018 10:17:03: 70000000 INFO @ Tue, 04 Sep 2018 10:17:03: 70000000 INFO @ Tue, 04 Sep 2018 10:17:09: 70000000 INFO @ Tue, 04 Sep 2018 10:17:10: 71000000 INFO @ Tue, 04 Sep 2018 10:17:10: 71000000 INFO @ Tue, 04 Sep 2018 10:17:16: 71000000 INFO @ Tue, 04 Sep 2018 10:17:17: 72000000 INFO @ Tue, 04 Sep 2018 10:17:17: 72000000 INFO @ Tue, 04 Sep 2018 10:17:24: 72000000 INFO @ Tue, 04 Sep 2018 10:17:25: 73000000 INFO @ Tue, 04 Sep 2018 10:17:25: 73000000 INFO @ Tue, 04 Sep 2018 10:17:31: 73000000 INFO @ Tue, 04 Sep 2018 10:17:32: 74000000 INFO @ Tue, 04 Sep 2018 10:17:32: 74000000 INFO @ Tue, 04 Sep 2018 10:17:38: 74000000 INFO @ Tue, 04 Sep 2018 10:17:39: 75000000 INFO @ Tue, 04 Sep 2018 10:17:39: 75000000 INFO @ Tue, 04 Sep 2018 10:17:46: 75000000 INFO @ Tue, 04 Sep 2018 10:17:47: 76000000 INFO @ Tue, 04 Sep 2018 10:17:47: 76000000 INFO @ Tue, 04 Sep 2018 10:17:53: 76000000 INFO @ Tue, 04 Sep 2018 10:17:54: 77000000 INFO @ Tue, 04 Sep 2018 10:17:54: 77000000 INFO @ Tue, 04 Sep 2018 10:17:54: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:17:54: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:17:54: #1 total tags in treatment: 37374431 INFO @ Tue, 04 Sep 2018 10:17:54: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:17:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:17:54: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:17:54: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:17:54: #1 total tags in treatment: 37374431 INFO @ Tue, 04 Sep 2018 10:17:54: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:17:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:17:55: #1 tags after filtering in treatment: 32294062 INFO @ Tue, 04 Sep 2018 10:17:55: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 04 Sep 2018 10:17:55: #1 finished! INFO @ Tue, 04 Sep 2018 10:17:55: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:17:55: #1 tags after filtering in treatment: 32294062 INFO @ Tue, 04 Sep 2018 10:17:55: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 04 Sep 2018 10:17:55: #1 finished! INFO @ Tue, 04 Sep 2018 10:17:55: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:17:57: #2 number of paired peaks: 67 WARNING @ Tue, 04 Sep 2018 10:17:57: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:17:57: Process for pairing-model is terminated! cat: SRX4012479.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012479.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012479.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012479.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 04 Sep 2018 10:17:57: #2 number of paired peaks: 67 WARNING @ Tue, 04 Sep 2018 10:17:57: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:17:57: Process for pairing-model is terminated! cat: SRX4012479.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012479.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012479.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012479.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 04 Sep 2018 10:18:00: 77000000 INFO @ Tue, 04 Sep 2018 10:18:01: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:18:01: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:18:01: #1 total tags in treatment: 37374431 INFO @ Tue, 04 Sep 2018 10:18:01: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:18:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:18:01: #1 tags after filtering in treatment: 32294062 INFO @ Tue, 04 Sep 2018 10:18:01: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 04 Sep 2018 10:18:01: #1 finished! INFO @ Tue, 04 Sep 2018 10:18:01: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:18:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:18:03: #2 number of paired peaks: 67 WARNING @ Tue, 04 Sep 2018 10:18:03: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:18:03: Process for pairing-model is terminated! cat: SRX4012479.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012479.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012479.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012479.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。