Job ID = 11170849 sra ファイルのダウンロード中... Completed: 1061538K bytes transferred in 100 seconds (86125K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 39347896 spots for /home/okishinya/chipatlas/results/ce10/SRX3583349/SRR6494005.sra Written 39347896 spots for /home/okishinya/chipatlas/results/ce10/SRX3583349/SRR6494005.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:40 39347896 reads; of these: 39347896 (100.00%) were unpaired; of these: 746757 (1.90%) aligned 0 times 32263078 (81.99%) aligned exactly 1 time 6338061 (16.11%) aligned >1 times 98.10% overall alignment rate Time searching: 00:15:40 Overall time: 00:15:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6533772 / 38601139 = 0.1693 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 11:54:28: # Command line: callpeak -t SRX3583349.bam -f BAM -g ce -n SRX3583349.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3583349.05 # format = BAM # ChIP-seq file = ['SRX3583349.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 11:54:28: # Command line: callpeak -t SRX3583349.bam -f BAM -g ce -n SRX3583349.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3583349.20 # format = BAM # ChIP-seq file = ['SRX3583349.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 11:54:28: # Command line: callpeak -t SRX3583349.bam -f BAM -g ce -n SRX3583349.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3583349.10 # format = BAM # ChIP-seq file = ['SRX3583349.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 11:54:28: #1 read tag files... INFO @ Sat, 08 Sep 2018 11:54:28: #1 read tag files... INFO @ Sat, 08 Sep 2018 11:54:28: #1 read tag files... INFO @ Sat, 08 Sep 2018 11:54:28: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 11:54:28: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 11:54:28: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 11:54:35: 1000000 INFO @ Sat, 08 Sep 2018 11:54:35: 1000000 INFO @ Sat, 08 Sep 2018 11:54:35: 1000000 INFO @ Sat, 08 Sep 2018 11:54:43: 2000000 INFO @ Sat, 08 Sep 2018 11:54:43: 2000000 INFO @ Sat, 08 Sep 2018 11:54:43: 2000000 INFO @ Sat, 08 Sep 2018 11:54:50: 3000000 INFO @ Sat, 08 Sep 2018 11:54:50: 3000000 INFO @ Sat, 08 Sep 2018 11:54:50: 3000000 INFO @ Sat, 08 Sep 2018 11:54:57: 4000000 INFO @ Sat, 08 Sep 2018 11:54:57: 4000000 INFO @ Sat, 08 Sep 2018 11:54:57: 4000000 INFO @ Sat, 08 Sep 2018 11:55:04: 5000000 INFO @ Sat, 08 Sep 2018 11:55:04: 5000000 INFO @ Sat, 08 Sep 2018 11:55:04: 5000000 INFO @ Sat, 08 Sep 2018 11:55:11: 6000000 INFO @ Sat, 08 Sep 2018 11:55:12: 6000000 INFO @ Sat, 08 Sep 2018 11:55:12: 6000000 INFO @ Sat, 08 Sep 2018 11:55:18: 7000000 INFO @ Sat, 08 Sep 2018 11:55:19: 7000000 INFO @ Sat, 08 Sep 2018 11:55:19: 7000000 INFO @ Sat, 08 Sep 2018 11:55:25: 8000000 INFO @ Sat, 08 Sep 2018 11:55:26: 8000000 INFO @ Sat, 08 Sep 2018 11:55:26: 8000000 INFO @ Sat, 08 Sep 2018 11:55:32: 9000000 INFO @ Sat, 08 Sep 2018 11:55:33: 9000000 INFO @ Sat, 08 Sep 2018 11:55:33: 9000000 INFO @ Sat, 08 Sep 2018 11:55:39: 10000000 INFO @ Sat, 08 Sep 2018 11:55:40: 10000000 INFO @ Sat, 08 Sep 2018 11:55:40: 10000000 INFO @ Sat, 08 Sep 2018 11:55:46: 11000000 INFO @ Sat, 08 Sep 2018 11:55:47: 11000000 INFO @ Sat, 08 Sep 2018 11:55:47: 11000000 INFO @ Sat, 08 Sep 2018 11:55:53: 12000000 INFO @ Sat, 08 Sep 2018 11:55:54: 12000000 INFO @ Sat, 08 Sep 2018 11:55:54: 12000000 INFO @ Sat, 08 Sep 2018 11:56:00: 13000000 INFO @ Sat, 08 Sep 2018 11:56:02: 13000000 INFO @ Sat, 08 Sep 2018 11:56:02: 13000000 INFO @ Sat, 08 Sep 2018 11:56:07: 14000000 INFO @ Sat, 08 Sep 2018 11:56:09: 14000000 INFO @ Sat, 08 Sep 2018 11:56:09: 14000000 INFO @ Sat, 08 Sep 2018 11:56:13: 15000000 INFO @ Sat, 08 Sep 2018 11:56:16: 15000000 INFO @ Sat, 08 Sep 2018 11:56:16: 15000000 INFO @ Sat, 08 Sep 2018 11:56:20: 16000000 INFO @ Sat, 08 Sep 2018 11:56:23: 16000000 INFO @ Sat, 08 Sep 2018 11:56:23: 16000000 INFO @ Sat, 08 Sep 2018 11:56:27: 17000000 INFO @ Sat, 08 Sep 2018 11:56:30: 17000000 INFO @ Sat, 08 Sep 2018 11:56:30: 17000000 INFO @ Sat, 08 Sep 2018 11:56:34: 18000000 INFO @ Sat, 08 Sep 2018 11:56:37: 18000000 INFO @ Sat, 08 Sep 2018 11:56:37: 18000000 INFO @ Sat, 08 Sep 2018 11:56:41: 19000000 INFO @ Sat, 08 Sep 2018 11:56:45: 19000000 INFO @ Sat, 08 Sep 2018 11:56:45: 19000000 INFO @ Sat, 08 Sep 2018 11:56:48: 20000000 INFO @ Sat, 08 Sep 2018 11:56:52: 20000000 INFO @ Sat, 08 Sep 2018 11:56:52: 20000000 INFO @ Sat, 08 Sep 2018 11:56:55: 21000000 INFO @ Sat, 08 Sep 2018 11:56:59: 21000000 INFO @ Sat, 08 Sep 2018 11:56:59: 21000000 INFO @ Sat, 08 Sep 2018 11:57:02: 22000000 INFO @ Sat, 08 Sep 2018 11:57:06: 22000000 INFO @ Sat, 08 Sep 2018 11:57:06: 22000000 INFO @ Sat, 08 Sep 2018 11:57:09: 23000000 INFO @ Sat, 08 Sep 2018 11:57:13: 23000000 INFO @ Sat, 08 Sep 2018 11:57:13: 23000000 INFO @ Sat, 08 Sep 2018 11:57:16: 24000000 INFO @ Sat, 08 Sep 2018 11:57:20: 24000000 INFO @ Sat, 08 Sep 2018 11:57:20: 24000000 INFO @ Sat, 08 Sep 2018 11:57:22: 25000000 INFO @ Sat, 08 Sep 2018 11:57:27: 25000000 INFO @ Sat, 08 Sep 2018 11:57:27: 25000000 INFO @ Sat, 08 Sep 2018 11:57:29: 26000000 INFO @ Sat, 08 Sep 2018 11:57:34: 26000000 INFO @ Sat, 08 Sep 2018 11:57:34: 26000000 INFO @ Sat, 08 Sep 2018 11:57:36: 27000000 INFO @ Sat, 08 Sep 2018 11:57:41: 27000000 INFO @ Sat, 08 Sep 2018 11:57:41: 27000000 INFO @ Sat, 08 Sep 2018 11:57:43: 28000000 INFO @ Sat, 08 Sep 2018 11:57:48: 28000000 INFO @ Sat, 08 Sep 2018 11:57:48: 28000000 INFO @ Sat, 08 Sep 2018 11:57:50: 29000000 INFO @ Sat, 08 Sep 2018 11:57:55: 29000000 INFO @ Sat, 08 Sep 2018 11:57:55: 29000000 INFO @ Sat, 08 Sep 2018 11:57:57: 30000000 INFO @ Sat, 08 Sep 2018 11:58:02: 30000000 INFO @ Sat, 08 Sep 2018 11:58:02: 30000000 INFO @ Sat, 08 Sep 2018 11:58:04: 31000000 INFO @ Sat, 08 Sep 2018 11:58:09: 31000000 INFO @ Sat, 08 Sep 2018 11:58:09: 31000000 INFO @ Sat, 08 Sep 2018 11:58:11: 32000000 INFO @ Sat, 08 Sep 2018 11:58:12: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 11:58:12: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 11:58:12: #1 total tags in treatment: 32067367 INFO @ Sat, 08 Sep 2018 11:58:12: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 11:58:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 11:58:12: #1 tags after filtering in treatment: 32067367 INFO @ Sat, 08 Sep 2018 11:58:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 11:58:12: #1 finished! INFO @ Sat, 08 Sep 2018 11:58:12: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 11:58:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 11:58:14: #2 number of paired peaks: 30 WARNING @ Sat, 08 Sep 2018 11:58:14: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 11:58:14: Process for pairing-model is terminated! cat: SRX3583349.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3583349.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583349.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583349.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 11:58:16: 32000000 INFO @ Sat, 08 Sep 2018 11:58:16: 32000000 INFO @ Sat, 08 Sep 2018 11:58:17: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 11:58:17: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 11:58:17: #1 total tags in treatment: 32067367 INFO @ Sat, 08 Sep 2018 11:58:17: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 11:58:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 11:58:17: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 11:58:17: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 11:58:17: #1 total tags in treatment: 32067367 INFO @ Sat, 08 Sep 2018 11:58:17: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 11:58:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 11:58:18: #1 tags after filtering in treatment: 32067367 INFO @ Sat, 08 Sep 2018 11:58:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 11:58:18: #1 finished! INFO @ Sat, 08 Sep 2018 11:58:18: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 11:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 11:58:18: #1 tags after filtering in treatment: 32067367 INFO @ Sat, 08 Sep 2018 11:58:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 11:58:18: #1 finished! INFO @ Sat, 08 Sep 2018 11:58:18: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 11:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 11:58:20: #2 number of paired peaks: 30 WARNING @ Sat, 08 Sep 2018 11:58:20: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 11:58:20: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 11:58:20: #2 number of paired peaks: 30 WARNING @ Sat, 08 Sep 2018 11:58:20: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 11:58:20: Process for pairing-model is terminated! cat: SRX3583349.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3583349.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583349.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583349.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません cat: SRX3583349.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3583349.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583349.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583349.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。