Job ID = 2589896 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,101,176 reads read : 3,101,176 reads written : 3,101,176 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947537.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 3101176 reads; of these: 3101176 (100.00%) were unpaired; of these: 699964 (22.57%) aligned 0 times 2141548 (69.06%) aligned exactly 1 time 259664 (8.37%) aligned >1 times 77.43% overall alignment rate Time searching: 00:00:30 Overall time: 00:00:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 357458 / 2401212 = 0.1489 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:43:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:43:02: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:43:02: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:43:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:43:03: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:43:03: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:43:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:43:05: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:43:05: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:43:09: 1000000 INFO @ Mon, 12 Aug 2019 18:43:10: 1000000 INFO @ Mon, 12 Aug 2019 18:43:12: 1000000 INFO @ Mon, 12 Aug 2019 18:43:16: 2000000 INFO @ Mon, 12 Aug 2019 18:43:16: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:43:16: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:43:16: #1 total tags in treatment: 2043754 INFO @ Mon, 12 Aug 2019 18:43:16: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:43:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:43:16: #1 tags after filtering in treatment: 2043754 INFO @ Mon, 12 Aug 2019 18:43:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:43:16: #1 finished! INFO @ Mon, 12 Aug 2019 18:43:16: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:43:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:43:17: #2 number of paired peaks: 3649 INFO @ Mon, 12 Aug 2019 18:43:17: start model_add_line... INFO @ Mon, 12 Aug 2019 18:43:17: start X-correlation... INFO @ Mon, 12 Aug 2019 18:43:17: end of X-cor INFO @ Mon, 12 Aug 2019 18:43:17: #2 finished! INFO @ Mon, 12 Aug 2019 18:43:17: #2 predicted fragment length is 170 bps INFO @ Mon, 12 Aug 2019 18:43:17: #2 alternative fragment length(s) may be 170 bps INFO @ Mon, 12 Aug 2019 18:43:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.05_model.r INFO @ Mon, 12 Aug 2019 18:43:17: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:43:17: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:43:17: 2000000 INFO @ Mon, 12 Aug 2019 18:43:17: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:43:17: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:43:17: #1 total tags in treatment: 2043754 INFO @ Mon, 12 Aug 2019 18:43:17: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:43:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:43:17: #1 tags after filtering in treatment: 2043754 INFO @ Mon, 12 Aug 2019 18:43:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:43:17: #1 finished! INFO @ Mon, 12 Aug 2019 18:43:17: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:43:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:43:18: #2 number of paired peaks: 3649 INFO @ Mon, 12 Aug 2019 18:43:18: start model_add_line... INFO @ Mon, 12 Aug 2019 18:43:18: start X-correlation... INFO @ Mon, 12 Aug 2019 18:43:18: end of X-cor INFO @ Mon, 12 Aug 2019 18:43:18: #2 finished! INFO @ Mon, 12 Aug 2019 18:43:18: #2 predicted fragment length is 170 bps INFO @ Mon, 12 Aug 2019 18:43:18: #2 alternative fragment length(s) may be 170 bps INFO @ Mon, 12 Aug 2019 18:43:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.10_model.r INFO @ Mon, 12 Aug 2019 18:43:18: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:43:18: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:43:18: 2000000 INFO @ Mon, 12 Aug 2019 18:43:18: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:43:18: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:43:18: #1 total tags in treatment: 2043754 INFO @ Mon, 12 Aug 2019 18:43:18: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:43:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:43:18: #1 tags after filtering in treatment: 2043754 INFO @ Mon, 12 Aug 2019 18:43:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:43:18: #1 finished! INFO @ Mon, 12 Aug 2019 18:43:18: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:43:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:43:19: #2 number of paired peaks: 3649 INFO @ Mon, 12 Aug 2019 18:43:19: start model_add_line... INFO @ Mon, 12 Aug 2019 18:43:19: start X-correlation... INFO @ Mon, 12 Aug 2019 18:43:19: end of X-cor INFO @ Mon, 12 Aug 2019 18:43:19: #2 finished! INFO @ Mon, 12 Aug 2019 18:43:19: #2 predicted fragment length is 170 bps INFO @ Mon, 12 Aug 2019 18:43:19: #2 alternative fragment length(s) may be 170 bps INFO @ Mon, 12 Aug 2019 18:43:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.20_model.r INFO @ Mon, 12 Aug 2019 18:43:19: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:43:19: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:43:24: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:43:25: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:43:26: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:43:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:43:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:43:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.05_summits.bed INFO @ Mon, 12 Aug 2019 18:43:27: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6068 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:43:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:43:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:43:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.10_summits.bed INFO @ Mon, 12 Aug 2019 18:43:28: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (3108 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:43:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:43:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:43:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331300/SRX331300.20_summits.bed INFO @ Mon, 12 Aug 2019 18:43:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1091 records, 4 fields): 7 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。