Job ID = 2589851 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,767,192 reads read : 5,767,192 reads written : 5,767,192 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947484.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:48 5767192 reads; of these: 5767192 (100.00%) were unpaired; of these: 2091026 (36.26%) aligned 0 times 3064276 (53.13%) aligned exactly 1 time 611890 (10.61%) aligned >1 times 63.74% overall alignment rate Time searching: 00:00:48 Overall time: 00:00:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 397117 / 3676166 = 0.1080 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:37:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:37:42: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:37:42: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:37:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:37:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:37:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:37:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:37:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:37:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:37:49: 1000000 INFO @ Mon, 12 Aug 2019 18:37:52: 1000000 INFO @ Mon, 12 Aug 2019 18:37:54: 1000000 INFO @ Mon, 12 Aug 2019 18:37:57: 2000000 INFO @ Mon, 12 Aug 2019 18:37:59: 2000000 INFO @ Mon, 12 Aug 2019 18:38:03: 2000000 INFO @ Mon, 12 Aug 2019 18:38:05: 3000000 INFO @ Mon, 12 Aug 2019 18:38:07: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:38:07: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:38:07: #1 total tags in treatment: 3279049 INFO @ Mon, 12 Aug 2019 18:38:07: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:38:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:38:07: #1 tags after filtering in treatment: 3279049 INFO @ Mon, 12 Aug 2019 18:38:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:38:07: #1 finished! INFO @ Mon, 12 Aug 2019 18:38:07: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:38:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:38:07: 3000000 INFO @ Mon, 12 Aug 2019 18:38:07: #2 number of paired peaks: 651 WARNING @ Mon, 12 Aug 2019 18:38:07: Fewer paired peaks (651) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 651 pairs to build model! INFO @ Mon, 12 Aug 2019 18:38:07: start model_add_line... INFO @ Mon, 12 Aug 2019 18:38:08: start X-correlation... INFO @ Mon, 12 Aug 2019 18:38:08: end of X-cor INFO @ Mon, 12 Aug 2019 18:38:08: #2 finished! INFO @ Mon, 12 Aug 2019 18:38:08: #2 predicted fragment length is 85 bps INFO @ Mon, 12 Aug 2019 18:38:08: #2 alternative fragment length(s) may be 4,53,85 bps INFO @ Mon, 12 Aug 2019 18:38:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.05_model.r INFO @ Mon, 12 Aug 2019 18:38:08: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:38:08: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:38:09: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:38:09: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:38:09: #1 total tags in treatment: 3279049 INFO @ Mon, 12 Aug 2019 18:38:09: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:38:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:38:09: #1 tags after filtering in treatment: 3279049 INFO @ Mon, 12 Aug 2019 18:38:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:38:09: #1 finished! INFO @ Mon, 12 Aug 2019 18:38:09: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:38:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:38:09: #2 number of paired peaks: 651 WARNING @ Mon, 12 Aug 2019 18:38:09: Fewer paired peaks (651) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 651 pairs to build model! INFO @ Mon, 12 Aug 2019 18:38:09: start model_add_line... INFO @ Mon, 12 Aug 2019 18:38:09: start X-correlation... INFO @ Mon, 12 Aug 2019 18:38:10: end of X-cor INFO @ Mon, 12 Aug 2019 18:38:10: #2 finished! INFO @ Mon, 12 Aug 2019 18:38:10: #2 predicted fragment length is 85 bps INFO @ Mon, 12 Aug 2019 18:38:10: #2 alternative fragment length(s) may be 4,53,85 bps INFO @ Mon, 12 Aug 2019 18:38:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.10_model.r INFO @ Mon, 12 Aug 2019 18:38:10: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:38:10: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:38:12: 3000000 INFO @ Mon, 12 Aug 2019 18:38:15: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:38:15: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:38:15: #1 total tags in treatment: 3279049 INFO @ Mon, 12 Aug 2019 18:38:15: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:38:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:38:15: #1 tags after filtering in treatment: 3279049 INFO @ Mon, 12 Aug 2019 18:38:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:38:15: #1 finished! INFO @ Mon, 12 Aug 2019 18:38:15: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:38:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:38:15: #2 number of paired peaks: 651 WARNING @ Mon, 12 Aug 2019 18:38:15: Fewer paired peaks (651) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 651 pairs to build model! INFO @ Mon, 12 Aug 2019 18:38:15: start model_add_line... INFO @ Mon, 12 Aug 2019 18:38:15: start X-correlation... INFO @ Mon, 12 Aug 2019 18:38:15: end of X-cor INFO @ Mon, 12 Aug 2019 18:38:15: #2 finished! INFO @ Mon, 12 Aug 2019 18:38:15: #2 predicted fragment length is 85 bps INFO @ Mon, 12 Aug 2019 18:38:15: #2 alternative fragment length(s) may be 4,53,85 bps INFO @ Mon, 12 Aug 2019 18:38:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.20_model.r INFO @ Mon, 12 Aug 2019 18:38:15: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:38:15: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:38:18: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:38:20: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:38:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:38:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:38:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.05_summits.bed INFO @ Mon, 12 Aug 2019 18:38:23: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1271 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:38:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:38:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:38:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.10_summits.bed INFO @ Mon, 12 Aug 2019 18:38:25: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (395 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:38:25: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:38:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:38:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:38:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331252/SRX331252.20_summits.bed INFO @ Mon, 12 Aug 2019 18:38:30: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (124 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。