Job ID = 2589785 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,193,516 reads read : 8,193,516 reads written : 8,193,516 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947401.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:31 8193516 reads; of these: 8193516 (100.00%) were unpaired; of these: 41335 (0.50%) aligned 0 times 6952446 (84.85%) aligned exactly 1 time 1199735 (14.64%) aligned >1 times 99.50% overall alignment rate Time searching: 00:01:31 Overall time: 00:01:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 841844 / 8152181 = 0.1033 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:30:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:30:42: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:30:42: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:30:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:30:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:30:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:30:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:30:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:30:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:30:50: 1000000 INFO @ Mon, 12 Aug 2019 18:30:50: 1000000 INFO @ Mon, 12 Aug 2019 18:30:52: 1000000 INFO @ Mon, 12 Aug 2019 18:30:57: 2000000 INFO @ Mon, 12 Aug 2019 18:30:57: 2000000 INFO @ Mon, 12 Aug 2019 18:31:00: 2000000 INFO @ Mon, 12 Aug 2019 18:31:04: 3000000 INFO @ Mon, 12 Aug 2019 18:31:05: 3000000 INFO @ Mon, 12 Aug 2019 18:31:08: 3000000 INFO @ Mon, 12 Aug 2019 18:31:10: 4000000 INFO @ Mon, 12 Aug 2019 18:31:12: 4000000 INFO @ Mon, 12 Aug 2019 18:31:16: 4000000 INFO @ Mon, 12 Aug 2019 18:31:17: 5000000 INFO @ Mon, 12 Aug 2019 18:31:20: 5000000 INFO @ Mon, 12 Aug 2019 18:31:23: 6000000 INFO @ Mon, 12 Aug 2019 18:31:24: 5000000 INFO @ Mon, 12 Aug 2019 18:31:28: 6000000 INFO @ Mon, 12 Aug 2019 18:31:30: 7000000 INFO @ Mon, 12 Aug 2019 18:31:32: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:31:32: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:31:32: #1 total tags in treatment: 7310337 INFO @ Mon, 12 Aug 2019 18:31:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:31:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:31:32: #1 tags after filtering in treatment: 7310337 INFO @ Mon, 12 Aug 2019 18:31:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:31:32: #1 finished! INFO @ Mon, 12 Aug 2019 18:31:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:31:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:31:32: 6000000 INFO @ Mon, 12 Aug 2019 18:31:32: #2 number of paired peaks: 332 WARNING @ Mon, 12 Aug 2019 18:31:32: Fewer paired peaks (332) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 332 pairs to build model! INFO @ Mon, 12 Aug 2019 18:31:32: start model_add_line... INFO @ Mon, 12 Aug 2019 18:31:32: start X-correlation... INFO @ Mon, 12 Aug 2019 18:31:32: end of X-cor INFO @ Mon, 12 Aug 2019 18:31:32: #2 finished! INFO @ Mon, 12 Aug 2019 18:31:32: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:31:32: #2 alternative fragment length(s) may be 2,31,586 bps INFO @ Mon, 12 Aug 2019 18:31:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.10_model.r WARNING @ Mon, 12 Aug 2019 18:31:32: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:31:32: #2 You may need to consider one of the other alternative d(s): 2,31,586 WARNING @ Mon, 12 Aug 2019 18:31:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:31:32: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:31:32: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:31:35: 7000000 INFO @ Mon, 12 Aug 2019 18:31:37: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:31:37: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:31:37: #1 total tags in treatment: 7310337 INFO @ Mon, 12 Aug 2019 18:31:37: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:31:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:31:37: #1 tags after filtering in treatment: 7310337 INFO @ Mon, 12 Aug 2019 18:31:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:31:37: #1 finished! INFO @ Mon, 12 Aug 2019 18:31:37: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:31:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:31:38: #2 number of paired peaks: 332 WARNING @ Mon, 12 Aug 2019 18:31:38: Fewer paired peaks (332) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 332 pairs to build model! INFO @ Mon, 12 Aug 2019 18:31:38: start model_add_line... INFO @ Mon, 12 Aug 2019 18:31:38: start X-correlation... INFO @ Mon, 12 Aug 2019 18:31:38: end of X-cor INFO @ Mon, 12 Aug 2019 18:31:38: #2 finished! INFO @ Mon, 12 Aug 2019 18:31:38: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:31:38: #2 alternative fragment length(s) may be 2,31,586 bps INFO @ Mon, 12 Aug 2019 18:31:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.05_model.r WARNING @ Mon, 12 Aug 2019 18:31:38: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:31:38: #2 You may need to consider one of the other alternative d(s): 2,31,586 WARNING @ Mon, 12 Aug 2019 18:31:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:31:38: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:31:38: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:31:40: 7000000 INFO @ Mon, 12 Aug 2019 18:31:42: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:31:42: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:31:42: #1 total tags in treatment: 7310337 INFO @ Mon, 12 Aug 2019 18:31:42: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:31:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:31:42: #1 tags after filtering in treatment: 7310337 INFO @ Mon, 12 Aug 2019 18:31:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:31:42: #1 finished! INFO @ Mon, 12 Aug 2019 18:31:42: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:31:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:31:43: #2 number of paired peaks: 332 WARNING @ Mon, 12 Aug 2019 18:31:43: Fewer paired peaks (332) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 332 pairs to build model! INFO @ Mon, 12 Aug 2019 18:31:43: start model_add_line... INFO @ Mon, 12 Aug 2019 18:31:43: start X-correlation... INFO @ Mon, 12 Aug 2019 18:31:43: end of X-cor INFO @ Mon, 12 Aug 2019 18:31:43: #2 finished! INFO @ Mon, 12 Aug 2019 18:31:43: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:31:43: #2 alternative fragment length(s) may be 2,31,586 bps INFO @ Mon, 12 Aug 2019 18:31:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.20_model.r WARNING @ Mon, 12 Aug 2019 18:31:43: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:31:43: #2 You may need to consider one of the other alternative d(s): 2,31,586 WARNING @ Mon, 12 Aug 2019 18:31:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:31:43: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:31:43: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:31:53: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:31:58: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:32:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:32:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:32:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.10_summits.bed INFO @ Mon, 12 Aug 2019 18:32:02: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (246 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:32:03: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:32:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:32:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:32:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.05_summits.bed INFO @ Mon, 12 Aug 2019 18:32:08: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (548 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:32:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:32:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:32:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331169/SRX331169.20_summits.bed INFO @ Mon, 12 Aug 2019 18:32:13: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (58 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。