Job ID = 2589777 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,506,637 reads read : 2,506,637 reads written : 2,506,637 spots read : 1,020,157 reads read : 1,020,157 reads written : 1,020,157 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947389.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947390.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:24 3526794 reads; of these: 3526794 (100.00%) were unpaired; of these: 2153719 (61.07%) aligned 0 times 1173716 (33.28%) aligned exactly 1 time 199359 (5.65%) aligned >1 times 38.93% overall alignment rate Time searching: 00:00:24 Overall time: 00:00:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 297866 / 1373075 = 0.2169 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:27:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:27:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:27:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:27:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:27:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:27:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:27:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:27:34: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:27:34: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:27:38: 1000000 INFO @ Mon, 12 Aug 2019 18:27:39: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:27:39: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:27:39: #1 total tags in treatment: 1075209 INFO @ Mon, 12 Aug 2019 18:27:39: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:27:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:27:39: #1 tags after filtering in treatment: 1075209 INFO @ Mon, 12 Aug 2019 18:27:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:27:39: #1 finished! INFO @ Mon, 12 Aug 2019 18:27:39: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:27:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:27:39: #2 number of paired peaks: 735 WARNING @ Mon, 12 Aug 2019 18:27:39: Fewer paired peaks (735) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 735 pairs to build model! INFO @ Mon, 12 Aug 2019 18:27:39: start model_add_line... INFO @ Mon, 12 Aug 2019 18:27:39: start X-correlation... INFO @ Mon, 12 Aug 2019 18:27:39: end of X-cor INFO @ Mon, 12 Aug 2019 18:27:39: #2 finished! INFO @ Mon, 12 Aug 2019 18:27:39: #2 predicted fragment length is 120 bps INFO @ Mon, 12 Aug 2019 18:27:39: #2 alternative fragment length(s) may be 120 bps INFO @ Mon, 12 Aug 2019 18:27:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.05_model.r INFO @ Mon, 12 Aug 2019 18:27:39: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:27:39: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:27:39: 1000000 INFO @ Mon, 12 Aug 2019 18:27:40: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:27:40: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:27:40: #1 total tags in treatment: 1075209 INFO @ Mon, 12 Aug 2019 18:27:40: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:27:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:27:40: #1 tags after filtering in treatment: 1075209 INFO @ Mon, 12 Aug 2019 18:27:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:27:40: #1 finished! INFO @ Mon, 12 Aug 2019 18:27:40: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:27:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:27:40: #2 number of paired peaks: 735 WARNING @ Mon, 12 Aug 2019 18:27:40: Fewer paired peaks (735) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 735 pairs to build model! INFO @ Mon, 12 Aug 2019 18:27:40: start model_add_line... INFO @ Mon, 12 Aug 2019 18:27:40: start X-correlation... INFO @ Mon, 12 Aug 2019 18:27:40: end of X-cor INFO @ Mon, 12 Aug 2019 18:27:40: #2 finished! INFO @ Mon, 12 Aug 2019 18:27:40: #2 predicted fragment length is 120 bps INFO @ Mon, 12 Aug 2019 18:27:40: #2 alternative fragment length(s) may be 120 bps INFO @ Mon, 12 Aug 2019 18:27:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.10_model.r INFO @ Mon, 12 Aug 2019 18:27:40: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:27:40: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:27:41: 1000000 INFO @ Mon, 12 Aug 2019 18:27:41: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:27:41: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:27:41: #1 total tags in treatment: 1075209 INFO @ Mon, 12 Aug 2019 18:27:41: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:27:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:27:41: #1 tags after filtering in treatment: 1075209 INFO @ Mon, 12 Aug 2019 18:27:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:27:41: #1 finished! INFO @ Mon, 12 Aug 2019 18:27:41: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:27:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:27:42: #2 number of paired peaks: 735 WARNING @ Mon, 12 Aug 2019 18:27:42: Fewer paired peaks (735) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 735 pairs to build model! INFO @ Mon, 12 Aug 2019 18:27:42: start model_add_line... INFO @ Mon, 12 Aug 2019 18:27:42: start X-correlation... INFO @ Mon, 12 Aug 2019 18:27:42: end of X-cor INFO @ Mon, 12 Aug 2019 18:27:42: #2 finished! INFO @ Mon, 12 Aug 2019 18:27:42: #2 predicted fragment length is 120 bps INFO @ Mon, 12 Aug 2019 18:27:42: #2 alternative fragment length(s) may be 120 bps INFO @ Mon, 12 Aug 2019 18:27:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.20_model.r INFO @ Mon, 12 Aug 2019 18:27:42: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:27:42: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:27:42: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:27:43: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:27:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:27:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:27:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.05_summits.bed INFO @ Mon, 12 Aug 2019 18:27:44: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (576 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:27:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:27:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:27:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.10_summits.bed INFO @ Mon, 12 Aug 2019 18:27:45: Done! INFO @ Mon, 12 Aug 2019 18:27:45: #3 Call peaks for each chromosome... pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (198 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:27:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:27:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:27:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331158/SRX331158.20_summits.bed INFO @ Mon, 12 Aug 2019 18:27:47: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (45 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。