Job ID = 1292039 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,531,086 reads read : 17,531,086 reads written : 17,531,086 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:34 17531086 reads; of these: 17531086 (100.00%) were unpaired; of these: 1547617 (8.83%) aligned 0 times 14044270 (80.11%) aligned exactly 1 time 1939199 (11.06%) aligned >1 times 91.17% overall alignment rate Time searching: 00:05:34 Overall time: 00:05:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4076477 / 15983469 = 0.2550 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:32:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:32:08: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:32:08: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:32:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:32:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:32:08: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:32:08: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:32:08: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:32:08: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:32:17: 1000000 INFO @ Sun, 02 Jun 2019 17:32:17: 1000000 INFO @ Sun, 02 Jun 2019 17:32:17: 1000000 INFO @ Sun, 02 Jun 2019 17:32:26: 2000000 INFO @ Sun, 02 Jun 2019 17:32:26: 2000000 INFO @ Sun, 02 Jun 2019 17:32:26: 2000000 INFO @ Sun, 02 Jun 2019 17:32:35: 3000000 INFO @ Sun, 02 Jun 2019 17:32:35: 3000000 INFO @ Sun, 02 Jun 2019 17:32:35: 3000000 INFO @ Sun, 02 Jun 2019 17:32:44: 4000000 INFO @ Sun, 02 Jun 2019 17:32:44: 4000000 INFO @ Sun, 02 Jun 2019 17:32:44: 4000000 INFO @ Sun, 02 Jun 2019 17:32:52: 5000000 INFO @ Sun, 02 Jun 2019 17:32:52: 5000000 INFO @ Sun, 02 Jun 2019 17:32:52: 5000000 INFO @ Sun, 02 Jun 2019 17:33:01: 6000000 INFO @ Sun, 02 Jun 2019 17:33:01: 6000000 INFO @ Sun, 02 Jun 2019 17:33:01: 6000000 INFO @ Sun, 02 Jun 2019 17:33:10: 7000000 INFO @ Sun, 02 Jun 2019 17:33:10: 7000000 INFO @ Sun, 02 Jun 2019 17:33:10: 7000000 INFO @ Sun, 02 Jun 2019 17:33:18: 8000000 INFO @ Sun, 02 Jun 2019 17:33:18: 8000000 INFO @ Sun, 02 Jun 2019 17:33:19: 8000000 INFO @ Sun, 02 Jun 2019 17:33:27: 9000000 INFO @ Sun, 02 Jun 2019 17:33:27: 9000000 INFO @ Sun, 02 Jun 2019 17:33:27: 9000000 INFO @ Sun, 02 Jun 2019 17:33:36: 10000000 INFO @ Sun, 02 Jun 2019 17:33:36: 10000000 INFO @ Sun, 02 Jun 2019 17:33:36: 10000000 INFO @ Sun, 02 Jun 2019 17:33:44: 11000000 INFO @ Sun, 02 Jun 2019 17:33:45: 11000000 INFO @ Sun, 02 Jun 2019 17:33:45: 11000000 INFO @ Sun, 02 Jun 2019 17:33:52: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:33:52: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:33:52: #1 total tags in treatment: 11906992 INFO @ Sun, 02 Jun 2019 17:33:52: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:33:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:33:52: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:33:52: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:33:52: #1 total tags in treatment: 11906992 INFO @ Sun, 02 Jun 2019 17:33:52: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:33:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:33:52: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:33:52: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:33:52: #1 total tags in treatment: 11906992 INFO @ Sun, 02 Jun 2019 17:33:52: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:33:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:33:53: #1 tags after filtering in treatment: 11906992 INFO @ Sun, 02 Jun 2019 17:33:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:33:53: #1 finished! INFO @ Sun, 02 Jun 2019 17:33:53: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:33:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:33:53: #1 tags after filtering in treatment: 11906992 INFO @ Sun, 02 Jun 2019 17:33:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:33:53: #1 finished! INFO @ Sun, 02 Jun 2019 17:33:53: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:33:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:33:53: #1 tags after filtering in treatment: 11906992 INFO @ Sun, 02 Jun 2019 17:33:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:33:53: #1 finished! INFO @ Sun, 02 Jun 2019 17:33:53: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:33:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:33:54: #2 number of paired peaks: 48 WARNING @ Sun, 02 Jun 2019 17:33:54: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:33:54: Process for pairing-model is terminated! INFO @ Sun, 02 Jun 2019 17:33:54: #2 number of paired peaks: 48 WARNING @ Sun, 02 Jun 2019 17:33:54: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:33:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) cut: /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.20_peaks.narrowPeak: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:33:54: #2 number of paired peaks: 48 WARNING @ Sun, 02 Jun 2019 17:33:54: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:33:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323685/SRX323685.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。