Job ID = 1292038 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,637,986 reads read : 12,637,986 reads written : 12,637,986 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:47 12637986 reads; of these: 12637986 (100.00%) were unpaired; of these: 1382280 (10.94%) aligned 0 times 10074886 (79.72%) aligned exactly 1 time 1180820 (9.34%) aligned >1 times 89.06% overall alignment rate Time searching: 00:03:48 Overall time: 00:03:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3183312 / 11255706 = 0.2828 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:25:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:25:11: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:25:11: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:25:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:25:11: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:25:11: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:25:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:25:11: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:25:11: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:25:18: 1000000 INFO @ Sun, 02 Jun 2019 17:25:20: 1000000 INFO @ Sun, 02 Jun 2019 17:25:20: 1000000 INFO @ Sun, 02 Jun 2019 17:25:25: 2000000 INFO @ Sun, 02 Jun 2019 17:25:28: 2000000 INFO @ Sun, 02 Jun 2019 17:25:29: 2000000 INFO @ Sun, 02 Jun 2019 17:25:32: 3000000 INFO @ Sun, 02 Jun 2019 17:25:36: 3000000 INFO @ Sun, 02 Jun 2019 17:25:37: 3000000 INFO @ Sun, 02 Jun 2019 17:25:40: 4000000 INFO @ Sun, 02 Jun 2019 17:25:44: 4000000 INFO @ Sun, 02 Jun 2019 17:25:45: 4000000 INFO @ Sun, 02 Jun 2019 17:25:46: 5000000 INFO @ Sun, 02 Jun 2019 17:25:52: 5000000 INFO @ Sun, 02 Jun 2019 17:25:53: 6000000 INFO @ Sun, 02 Jun 2019 17:25:54: 5000000 INFO @ Sun, 02 Jun 2019 17:26:00: 7000000 INFO @ Sun, 02 Jun 2019 17:26:00: 6000000 INFO @ Sun, 02 Jun 2019 17:26:02: 6000000 INFO @ Sun, 02 Jun 2019 17:26:07: 8000000 INFO @ Sun, 02 Jun 2019 17:26:08: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:26:08: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:26:08: #1 total tags in treatment: 8072394 INFO @ Sun, 02 Jun 2019 17:26:08: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:26:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:26:08: #1 tags after filtering in treatment: 8072394 INFO @ Sun, 02 Jun 2019 17:26:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:26:08: #1 finished! INFO @ Sun, 02 Jun 2019 17:26:08: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:26:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:26:08: 7000000 INFO @ Sun, 02 Jun 2019 17:26:08: #2 number of paired peaks: 51 WARNING @ Sun, 02 Jun 2019 17:26:08: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:26:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:26:10: 7000000 INFO @ Sun, 02 Jun 2019 17:26:16: 8000000 INFO @ Sun, 02 Jun 2019 17:26:17: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:26:17: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:26:17: #1 total tags in treatment: 8072394 INFO @ Sun, 02 Jun 2019 17:26:17: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:26:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:26:17: #1 tags after filtering in treatment: 8072394 INFO @ Sun, 02 Jun 2019 17:26:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:26:17: #1 finished! INFO @ Sun, 02 Jun 2019 17:26:17: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:26:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:26:18: 8000000 INFO @ Sun, 02 Jun 2019 17:26:18: #2 number of paired peaks: 51 WARNING @ Sun, 02 Jun 2019 17:26:18: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:26:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:26:19: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:26:19: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:26:19: #1 total tags in treatment: 8072394 INFO @ Sun, 02 Jun 2019 17:26:19: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:26:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:26:19: #1 tags after filtering in treatment: 8072394 INFO @ Sun, 02 Jun 2019 17:26:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:26:19: #1 finished! INFO @ Sun, 02 Jun 2019 17:26:19: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:26:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:26:19: #2 number of paired peaks: 51 WARNING @ Sun, 02 Jun 2019 17:26:19: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:26:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323684/SRX323684.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。