Job ID = 12264771 SRX = SRX3141082 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 86746540 spots for SRR5985088/SRR5985088.sra Written 86746540 spots for SRR5985088/SRR5985088.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265666 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:10:12 86746540 reads; of these: 86746540 (100.00%) were paired; of these: 53480285 (61.65%) aligned concordantly 0 times 26915669 (31.03%) aligned concordantly exactly 1 time 6350586 (7.32%) aligned concordantly >1 times ---- 53480285 pairs aligned concordantly 0 times; of these: 6089447 (11.39%) aligned discordantly 1 time ---- 47390838 pairs aligned 0 times concordantly or discordantly; of these: 94781676 mates make up the pairs; of these: 91020423 (96.03%) aligned 0 times 1439620 (1.52%) aligned exactly 1 time 2321633 (2.45%) aligned >1 times 47.54% overall alignment rate Time searching: 01:10:12 Overall time: 01:10:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 11193004 / 38911470 = 0.2877 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:06:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:06:47: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:06:47: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:06:53: 1000000 INFO @ Sat, 03 Apr 2021 08:06:59: 2000000 INFO @ Sat, 03 Apr 2021 08:07:05: 3000000 INFO @ Sat, 03 Apr 2021 08:07:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:07:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:07:17: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:07:17: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:07:17: 5000000 INFO @ Sat, 03 Apr 2021 08:07:24: 6000000 INFO @ Sat, 03 Apr 2021 08:07:24: 1000000 INFO @ Sat, 03 Apr 2021 08:07:30: 7000000 INFO @ Sat, 03 Apr 2021 08:07:31: 2000000 INFO @ Sat, 03 Apr 2021 08:07:36: 8000000 INFO @ Sat, 03 Apr 2021 08:07:38: 3000000 INFO @ Sat, 03 Apr 2021 08:07:43: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:07:45: 4000000 INFO @ Sat, 03 Apr 2021 08:07:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:07:47: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:07:47: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:07:49: 10000000 INFO @ Sat, 03 Apr 2021 08:07:53: 5000000 INFO @ Sat, 03 Apr 2021 08:07:55: 11000000 INFO @ Sat, 03 Apr 2021 08:07:55: 1000000 INFO @ Sat, 03 Apr 2021 08:08:01: 12000000 INFO @ Sat, 03 Apr 2021 08:08:02: 6000000 INFO @ Sat, 03 Apr 2021 08:08:03: 2000000 INFO @ Sat, 03 Apr 2021 08:08:07: 13000000 INFO @ Sat, 03 Apr 2021 08:08:10: 7000000 INFO @ Sat, 03 Apr 2021 08:08:12: 3000000 INFO @ Sat, 03 Apr 2021 08:08:14: 14000000 INFO @ Sat, 03 Apr 2021 08:08:19: 8000000 INFO @ Sat, 03 Apr 2021 08:08:20: 15000000 INFO @ Sat, 03 Apr 2021 08:08:21: 4000000 INFO @ Sat, 03 Apr 2021 08:08:26: 16000000 INFO @ Sat, 03 Apr 2021 08:08:27: 9000000 INFO @ Sat, 03 Apr 2021 08:08:29: 5000000 INFO @ Sat, 03 Apr 2021 08:08:33: 17000000 INFO @ Sat, 03 Apr 2021 08:08:35: 10000000 INFO @ Sat, 03 Apr 2021 08:08:37: 6000000 INFO @ Sat, 03 Apr 2021 08:08:39: 18000000 INFO @ Sat, 03 Apr 2021 08:08:43: 11000000 INFO @ Sat, 03 Apr 2021 08:08:45: 7000000 INFO @ Sat, 03 Apr 2021 08:08:46: 19000000 INFO @ Sat, 03 Apr 2021 08:08:51: 12000000 INFO @ Sat, 03 Apr 2021 08:08:52: 20000000 INFO @ Sat, 03 Apr 2021 08:08:53: 8000000 INFO @ Sat, 03 Apr 2021 08:08:58: 21000000 INFO @ Sat, 03 Apr 2021 08:08:59: 13000000 INFO @ Sat, 03 Apr 2021 08:09:01: 9000000 INFO @ Sat, 03 Apr 2021 08:09:05: 22000000 INFO @ Sat, 03 Apr 2021 08:09:08: 14000000 INFO @ Sat, 03 Apr 2021 08:09:09: 10000000 INFO @ Sat, 03 Apr 2021 08:09:11: 23000000 INFO @ Sat, 03 Apr 2021 08:09:16: 15000000 INFO @ Sat, 03 Apr 2021 08:09:16: 11000000 INFO @ Sat, 03 Apr 2021 08:09:18: 24000000 INFO @ Sat, 03 Apr 2021 08:09:24: 16000000 INFO @ Sat, 03 Apr 2021 08:09:24: 12000000 INFO @ Sat, 03 Apr 2021 08:09:24: 25000000 INFO @ Sat, 03 Apr 2021 08:09:31: 26000000 INFO @ Sat, 03 Apr 2021 08:09:32: 17000000 INFO @ Sat, 03 Apr 2021 08:09:32: 13000000 INFO @ Sat, 03 Apr 2021 08:09:37: 27000000 INFO @ Sat, 03 Apr 2021 08:09:39: 14000000 INFO @ Sat, 03 Apr 2021 08:09:39: 18000000 INFO @ Sat, 03 Apr 2021 08:09:43: 28000000 INFO @ Sat, 03 Apr 2021 08:09:47: 15000000 INFO @ Sat, 03 Apr 2021 08:09:48: 19000000 INFO @ Sat, 03 Apr 2021 08:09:50: 29000000 INFO @ Sat, 03 Apr 2021 08:09:55: 16000000 INFO @ Sat, 03 Apr 2021 08:09:55: 20000000 INFO @ Sat, 03 Apr 2021 08:09:57: 30000000 INFO @ Sat, 03 Apr 2021 08:10:03: 17000000 INFO @ Sat, 03 Apr 2021 08:10:03: 31000000 INFO @ Sat, 03 Apr 2021 08:10:04: 21000000 INFO @ Sat, 03 Apr 2021 08:10:10: 32000000 INFO @ Sat, 03 Apr 2021 08:10:11: 18000000 INFO @ Sat, 03 Apr 2021 08:10:12: 22000000 INFO @ Sat, 03 Apr 2021 08:10:16: 33000000 INFO @ Sat, 03 Apr 2021 08:10:18: 19000000 INFO @ Sat, 03 Apr 2021 08:10:20: 23000000 INFO @ Sat, 03 Apr 2021 08:10:22: 34000000 INFO @ Sat, 03 Apr 2021 08:10:26: 20000000 INFO @ Sat, 03 Apr 2021 08:10:28: 35000000 INFO @ Sat, 03 Apr 2021 08:10:29: 24000000 INFO @ Sat, 03 Apr 2021 08:10:34: 21000000 INFO @ Sat, 03 Apr 2021 08:10:35: 36000000 INFO @ Sat, 03 Apr 2021 08:10:38: 25000000 INFO @ Sat, 03 Apr 2021 08:10:41: 37000000 INFO @ Sat, 03 Apr 2021 08:10:43: 22000000 INFO @ Sat, 03 Apr 2021 08:10:46: 26000000 INFO @ Sat, 03 Apr 2021 08:10:48: 38000000 INFO @ Sat, 03 Apr 2021 08:10:51: 23000000 INFO @ Sat, 03 Apr 2021 08:10:54: 27000000 INFO @ Sat, 03 Apr 2021 08:10:54: 39000000 INFO @ Sat, 03 Apr 2021 08:10:59: 24000000 INFO @ Sat, 03 Apr 2021 08:11:00: 40000000 INFO @ Sat, 03 Apr 2021 08:11:02: 28000000 INFO @ Sat, 03 Apr 2021 08:11:07: 41000000 INFO @ Sat, 03 Apr 2021 08:11:07: 25000000 INFO @ Sat, 03 Apr 2021 08:11:10: 29000000 INFO @ Sat, 03 Apr 2021 08:11:13: 42000000 INFO @ Sat, 03 Apr 2021 08:11:15: 26000000 INFO @ Sat, 03 Apr 2021 08:11:18: 30000000 INFO @ Sat, 03 Apr 2021 08:11:20: 43000000 INFO @ Sat, 03 Apr 2021 08:11:23: 27000000 INFO @ Sat, 03 Apr 2021 08:11:26: 31000000 INFO @ Sat, 03 Apr 2021 08:11:26: 44000000 INFO @ Sat, 03 Apr 2021 08:11:31: 28000000 INFO @ Sat, 03 Apr 2021 08:11:32: 45000000 INFO @ Sat, 03 Apr 2021 08:11:34: 32000000 INFO @ Sat, 03 Apr 2021 08:11:39: 46000000 INFO @ Sat, 03 Apr 2021 08:11:40: 29000000 INFO @ Sat, 03 Apr 2021 08:11:43: 33000000 INFO @ Sat, 03 Apr 2021 08:11:45: 47000000 INFO @ Sat, 03 Apr 2021 08:11:48: 30000000 INFO @ Sat, 03 Apr 2021 08:11:51: 48000000 INFO @ Sat, 03 Apr 2021 08:11:51: 34000000 INFO @ Sat, 03 Apr 2021 08:11:56: 31000000 INFO @ Sat, 03 Apr 2021 08:11:58: 49000000 INFO @ Sat, 03 Apr 2021 08:12:00: 35000000 INFO @ Sat, 03 Apr 2021 08:12:04: 50000000 INFO @ Sat, 03 Apr 2021 08:12:05: 32000000 INFO @ Sat, 03 Apr 2021 08:12:09: 36000000 INFO @ Sat, 03 Apr 2021 08:12:11: 51000000 INFO @ Sat, 03 Apr 2021 08:12:13: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:12:17: 52000000 INFO @ Sat, 03 Apr 2021 08:12:17: 37000000 INFO @ Sat, 03 Apr 2021 08:12:22: 34000000 INFO @ Sat, 03 Apr 2021 08:12:23: 53000000 INFO @ Sat, 03 Apr 2021 08:12:25: 38000000 INFO @ Sat, 03 Apr 2021 08:12:29: 35000000 INFO @ Sat, 03 Apr 2021 08:12:30: 54000000 INFO @ Sat, 03 Apr 2021 08:12:33: 39000000 INFO @ Sat, 03 Apr 2021 08:12:36: 55000000 INFO @ Sat, 03 Apr 2021 08:12:37: 36000000 INFO @ Sat, 03 Apr 2021 08:12:41: 40000000 INFO @ Sat, 03 Apr 2021 08:12:43: 56000000 INFO @ Sat, 03 Apr 2021 08:12:45: 37000000 INFO @ Sat, 03 Apr 2021 08:12:49: 57000000 INFO @ Sat, 03 Apr 2021 08:12:50: 41000000 INFO @ Sat, 03 Apr 2021 08:12:53: 38000000 INFO @ Sat, 03 Apr 2021 08:12:56: 58000000 INFO @ Sat, 03 Apr 2021 08:12:58: 42000000 INFO @ Sat, 03 Apr 2021 08:13:01: 39000000 INFO @ Sat, 03 Apr 2021 08:13:03: 59000000 INFO @ Sat, 03 Apr 2021 08:13:06: 43000000 INFO @ Sat, 03 Apr 2021 08:13:09: 60000000 INFO @ Sat, 03 Apr 2021 08:13:09: 40000000 INFO @ Sat, 03 Apr 2021 08:13:10: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:13:10: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:13:10: #1 total tags in treatment: 23920612 INFO @ Sat, 03 Apr 2021 08:13:10: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:13:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:13:10: #1 tags after filtering in treatment: 15369773 INFO @ Sat, 03 Apr 2021 08:13:10: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 03 Apr 2021 08:13:10: #1 finished! INFO @ Sat, 03 Apr 2021 08:13:10: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:13:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:13:11: #2 number of paired peaks: 295 WARNING @ Sat, 03 Apr 2021 08:13:11: Fewer paired peaks (295) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 295 pairs to build model! INFO @ Sat, 03 Apr 2021 08:13:11: start model_add_line... INFO @ Sat, 03 Apr 2021 08:13:12: start X-correlation... INFO @ Sat, 03 Apr 2021 08:13:12: end of X-cor INFO @ Sat, 03 Apr 2021 08:13:12: #2 finished! INFO @ Sat, 03 Apr 2021 08:13:12: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 08:13:12: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 08:13:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.05_model.r WARNING @ Sat, 03 Apr 2021 08:13:12: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:13:12: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 08:13:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:13:12: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:13:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:13:14: 44000000 INFO @ Sat, 03 Apr 2021 08:13:18: 41000000 INFO @ Sat, 03 Apr 2021 08:13:23: 45000000 INFO @ Sat, 03 Apr 2021 08:13:26: 42000000 INFO @ Sat, 03 Apr 2021 08:13:31: 46000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:13:35: 43000000 INFO @ Sat, 03 Apr 2021 08:13:39: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:13:40: 47000000 INFO @ Sat, 03 Apr 2021 08:13:43: 44000000 INFO @ Sat, 03 Apr 2021 08:13:48: 48000000 INFO @ Sat, 03 Apr 2021 08:13:52: 45000000 INFO @ Sat, 03 Apr 2021 08:13:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:13:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:13:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.05_summits.bed INFO @ Sat, 03 Apr 2021 08:13:54: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10401 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:13:57: 49000000 INFO @ Sat, 03 Apr 2021 08:14:00: 46000000 INFO @ Sat, 03 Apr 2021 08:14:05: 50000000 INFO @ Sat, 03 Apr 2021 08:14:08: 47000000 INFO @ Sat, 03 Apr 2021 08:14:14: 51000000 INFO @ Sat, 03 Apr 2021 08:14:17: 48000000 INFO @ Sat, 03 Apr 2021 08:14:23: 52000000 INFO @ Sat, 03 Apr 2021 08:14:25: 49000000 INFO @ Sat, 03 Apr 2021 08:14:31: 53000000 INFO @ Sat, 03 Apr 2021 08:14:34: 50000000 INFO @ Sat, 03 Apr 2021 08:14:39: 54000000 INFO @ Sat, 03 Apr 2021 08:14:42: 51000000 INFO @ Sat, 03 Apr 2021 08:14:47: 55000000 INFO @ Sat, 03 Apr 2021 08:14:49: 52000000 INFO @ Sat, 03 Apr 2021 08:14:54: 56000000 INFO @ Sat, 03 Apr 2021 08:14:56: 53000000 INFO @ Sat, 03 Apr 2021 08:15:02: 57000000 INFO @ Sat, 03 Apr 2021 08:15:04: 54000000 INFO @ Sat, 03 Apr 2021 08:15:09: 58000000 INFO @ Sat, 03 Apr 2021 08:15:11: 55000000 INFO @ Sat, 03 Apr 2021 08:15:17: 59000000 INFO @ Sat, 03 Apr 2021 08:15:18: 56000000 INFO @ Sat, 03 Apr 2021 08:15:24: 60000000 INFO @ Sat, 03 Apr 2021 08:15:25: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:15:25: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:15:25: #1 total tags in treatment: 23920612 INFO @ Sat, 03 Apr 2021 08:15:25: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:15:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:15:25: 57000000 INFO @ Sat, 03 Apr 2021 08:15:25: #1 tags after filtering in treatment: 15369773 INFO @ Sat, 03 Apr 2021 08:15:25: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 03 Apr 2021 08:15:25: #1 finished! INFO @ Sat, 03 Apr 2021 08:15:25: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:15:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:15:27: #2 number of paired peaks: 295 WARNING @ Sat, 03 Apr 2021 08:15:27: Fewer paired peaks (295) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 295 pairs to build model! INFO @ Sat, 03 Apr 2021 08:15:27: start model_add_line... INFO @ Sat, 03 Apr 2021 08:15:27: start X-correlation... INFO @ Sat, 03 Apr 2021 08:15:27: end of X-cor INFO @ Sat, 03 Apr 2021 08:15:27: #2 finished! INFO @ Sat, 03 Apr 2021 08:15:27: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 08:15:27: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 08:15:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.10_model.r WARNING @ Sat, 03 Apr 2021 08:15:27: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:15:27: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 08:15:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:15:27: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:15:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:15:32: 58000000 INFO @ Sat, 03 Apr 2021 08:15:39: 59000000 INFO @ Sat, 03 Apr 2021 08:15:46: 60000000 INFO @ Sat, 03 Apr 2021 08:15:47: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:15:47: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:15:47: #1 total tags in treatment: 23920612 INFO @ Sat, 03 Apr 2021 08:15:47: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:15:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:15:47: #1 tags after filtering in treatment: 15369773 INFO @ Sat, 03 Apr 2021 08:15:47: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 03 Apr 2021 08:15:47: #1 finished! INFO @ Sat, 03 Apr 2021 08:15:47: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:15:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:15:48: #2 number of paired peaks: 295 WARNING @ Sat, 03 Apr 2021 08:15:48: Fewer paired peaks (295) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 295 pairs to build model! INFO @ Sat, 03 Apr 2021 08:15:48: start model_add_line... INFO @ Sat, 03 Apr 2021 08:15:49: start X-correlation... INFO @ Sat, 03 Apr 2021 08:15:49: end of X-cor INFO @ Sat, 03 Apr 2021 08:15:49: #2 finished! INFO @ Sat, 03 Apr 2021 08:15:49: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 08:15:49: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 08:15:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.20_model.r WARNING @ Sat, 03 Apr 2021 08:15:49: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:15:49: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 08:15:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:15:49: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:15:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:15:55: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:16:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:16:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:16:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.10_summits.bed INFO @ Sat, 03 Apr 2021 08:16:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (5536 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:16:17: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:16:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:16:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:16:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3141082/SRX3141082.20_summits.bed INFO @ Sat, 03 Apr 2021 08:16:33: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2164 records, 4 fields): 4 millis CompletedMACS2peakCalling