Job ID = 1292019 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,248,442 reads read : 64,496,884 reads written : 32,248,442 reads 0-length : 32,248,442 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:57 32248442 reads; of these: 32248442 (100.00%) were unpaired; of these: 668466 (2.07%) aligned 0 times 26370615 (81.77%) aligned exactly 1 time 5209361 (16.15%) aligned >1 times 97.93% overall alignment rate Time searching: 00:07:57 Overall time: 00:07:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3948560 / 31579976 = 0.1250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:31:42: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:31:42: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:31:42: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:31:42: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:31:42: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:31:42: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:31:51: 1000000 INFO @ Sun, 02 Jun 2019 17:31:51: 1000000 INFO @ Sun, 02 Jun 2019 17:31:53: 1000000 INFO @ Sun, 02 Jun 2019 17:32:00: 2000000 INFO @ Sun, 02 Jun 2019 17:32:00: 2000000 INFO @ Sun, 02 Jun 2019 17:32:03: 2000000 INFO @ Sun, 02 Jun 2019 17:32:08: 3000000 INFO @ Sun, 02 Jun 2019 17:32:09: 3000000 INFO @ Sun, 02 Jun 2019 17:32:13: 3000000 INFO @ Sun, 02 Jun 2019 17:32:16: 4000000 INFO @ Sun, 02 Jun 2019 17:32:17: 4000000 INFO @ Sun, 02 Jun 2019 17:32:23: 4000000 INFO @ Sun, 02 Jun 2019 17:32:25: 5000000 INFO @ Sun, 02 Jun 2019 17:32:26: 5000000 INFO @ Sun, 02 Jun 2019 17:32:33: 6000000 INFO @ Sun, 02 Jun 2019 17:32:34: 5000000 INFO @ Sun, 02 Jun 2019 17:32:35: 6000000 INFO @ Sun, 02 Jun 2019 17:32:41: 7000000 INFO @ Sun, 02 Jun 2019 17:32:43: 7000000 INFO @ Sun, 02 Jun 2019 17:32:44: 6000000 INFO @ Sun, 02 Jun 2019 17:32:49: 8000000 INFO @ Sun, 02 Jun 2019 17:32:52: 8000000 INFO @ Sun, 02 Jun 2019 17:32:54: 7000000 INFO @ Sun, 02 Jun 2019 17:32:59: 9000000 INFO @ Sun, 02 Jun 2019 17:33:00: 9000000 INFO @ Sun, 02 Jun 2019 17:33:05: 8000000 INFO @ Sun, 02 Jun 2019 17:33:07: 10000000 INFO @ Sun, 02 Jun 2019 17:33:09: 10000000 INFO @ Sun, 02 Jun 2019 17:33:15: 9000000 INFO @ Sun, 02 Jun 2019 17:33:15: 11000000 INFO @ Sun, 02 Jun 2019 17:33:18: 11000000 INFO @ Sun, 02 Jun 2019 17:33:23: 12000000 INFO @ Sun, 02 Jun 2019 17:33:25: 10000000 INFO @ Sun, 02 Jun 2019 17:33:26: 12000000 INFO @ Sun, 02 Jun 2019 17:33:33: 13000000 INFO @ Sun, 02 Jun 2019 17:33:35: 11000000 INFO @ Sun, 02 Jun 2019 17:33:35: 13000000 INFO @ Sun, 02 Jun 2019 17:33:42: 14000000 INFO @ Sun, 02 Jun 2019 17:33:44: 14000000 INFO @ Sun, 02 Jun 2019 17:33:45: 12000000 INFO @ Sun, 02 Jun 2019 17:33:50: 15000000 INFO @ Sun, 02 Jun 2019 17:33:53: 15000000 INFO @ Sun, 02 Jun 2019 17:33:55: 13000000 INFO @ Sun, 02 Jun 2019 17:33:58: 16000000 INFO @ Sun, 02 Jun 2019 17:34:01: 16000000 INFO @ Sun, 02 Jun 2019 17:34:05: 14000000 INFO @ Sun, 02 Jun 2019 17:34:07: 17000000 INFO @ Sun, 02 Jun 2019 17:34:09: 17000000 INFO @ Sun, 02 Jun 2019 17:34:14: 15000000 INFO @ Sun, 02 Jun 2019 17:34:15: 18000000 INFO @ Sun, 02 Jun 2019 17:34:18: 18000000 INFO @ Sun, 02 Jun 2019 17:34:23: 19000000 INFO @ Sun, 02 Jun 2019 17:34:24: 16000000 INFO @ Sun, 02 Jun 2019 17:34:26: 19000000 INFO @ Sun, 02 Jun 2019 17:34:32: 20000000 INFO @ Sun, 02 Jun 2019 17:34:34: 17000000 INFO @ Sun, 02 Jun 2019 17:34:35: 20000000 INFO @ Sun, 02 Jun 2019 17:34:40: 21000000 INFO @ Sun, 02 Jun 2019 17:34:43: 21000000 INFO @ Sun, 02 Jun 2019 17:34:44: 18000000 INFO @ Sun, 02 Jun 2019 17:34:48: 22000000 INFO @ Sun, 02 Jun 2019 17:34:51: 22000000 INFO @ Sun, 02 Jun 2019 17:34:54: 19000000 INFO @ Sun, 02 Jun 2019 17:34:56: 23000000 INFO @ Sun, 02 Jun 2019 17:34:59: 23000000 INFO @ Sun, 02 Jun 2019 17:35:03: 20000000 INFO @ Sun, 02 Jun 2019 17:35:04: 24000000 INFO @ Sun, 02 Jun 2019 17:35:07: 24000000 INFO @ Sun, 02 Jun 2019 17:35:13: 25000000 INFO @ Sun, 02 Jun 2019 17:35:13: 21000000 INFO @ Sun, 02 Jun 2019 17:35:16: 25000000 INFO @ Sun, 02 Jun 2019 17:35:21: 26000000 INFO @ Sun, 02 Jun 2019 17:35:23: 22000000 INFO @ Sun, 02 Jun 2019 17:35:24: 26000000 INFO @ Sun, 02 Jun 2019 17:35:29: 27000000 INFO @ Sun, 02 Jun 2019 17:35:32: 27000000 INFO @ Sun, 02 Jun 2019 17:35:33: 23000000 INFO @ Sun, 02 Jun 2019 17:35:35: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:35:35: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:35:35: #1 total tags in treatment: 27631416 INFO @ Sun, 02 Jun 2019 17:35:35: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:35:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:35:35: #1 tags after filtering in treatment: 27631416 INFO @ Sun, 02 Jun 2019 17:35:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:35:35: #1 finished! INFO @ Sun, 02 Jun 2019 17:35:35: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:35:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:35:37: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:35:37: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:35:37: #1 total tags in treatment: 27631416 INFO @ Sun, 02 Jun 2019 17:35:37: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:35:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:35:38: #2 number of paired peaks: 96 WARNING @ Sun, 02 Jun 2019 17:35:38: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:35:38: Process for pairing-model is terminated! INFO @ Sun, 02 Jun 2019 17:35:38: #1 tags after filtering in treatment: 27631416 INFO @ Sun, 02 Jun 2019 17:35:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:35:38: #1 finished! INFO @ Sun, 02 Jun 2019 17:35:38: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:35:38: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:35:40: #2 number of paired peaks: 96 WARNING @ Sun, 02 Jun 2019 17:35:40: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:35:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:35:42: 24000000 INFO @ Sun, 02 Jun 2019 17:35:52: 25000000 INFO @ Sun, 02 Jun 2019 17:36:02: 26000000 INFO @ Sun, 02 Jun 2019 17:36:11: 27000000 INFO @ Sun, 02 Jun 2019 17:36:17: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:36:17: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:36:17: #1 total tags in treatment: 27631416 INFO @ Sun, 02 Jun 2019 17:36:17: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:36:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:36:17: #1 tags after filtering in treatment: 27631416 INFO @ Sun, 02 Jun 2019 17:36:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:36:17: #1 finished! INFO @ Sun, 02 Jun 2019 17:36:17: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:36:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:36:20: #2 number of paired peaks: 96 WARNING @ Sun, 02 Jun 2019 17:36:20: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:36:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104609/SRX3104609.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。